About This Journal

About This Journal

Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provide scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.

 

2022 Impact Factor 1

 

  • Research Article

    Response of Photosynthetic Characteristics to Different Salicylic Acid Concentrations in Relation to Waterlogging Resistance in Zanthoxylum armatum
    Peiyun Wang, Chengyi Su, Jiaojiao Wu, Yuxin Xie, Jiangtao Fan, Jingyan Wang, Wenkai Hui, Hua Yang, Wei Gong
    Zanthoxylum armatum (Rutaceae) is an important spice and a traditional medicinal plant in southwest China. Owing to its shallow root system, it … + READ MORE
    Zanthoxylum armatum (Rutaceae) is an important spice and a traditional medicinal plant in southwest China. Owing to its shallow root system, it is highly susceptible to waterlogging stress. To evaluate how an exogenous salicylic acid (SA) application reduces photosynthetic damage in Z. armatum under waterlogging stress conditions, the content of photosynthetic pigments and gas exchange and chlorophyll fluorescence parameters were studied under different SA concentrations (0, 1 and 2 mM) and different numbers of waterlogging days (1, 2 and 3 d) to identify the mechanisms underlying the seedlings’ waterlogging response. The results showed that waterlogging significantly affects the photosynthetic characteristics in leaves. An exogenous SA application increased the contents of photosynthetic pigments as well as the transpiration rate (Tr), net leaf photosynthetic rate (Pn), stomatal conductance (Gs), water use efficiency (WUE), actual quantum efficiency (ΦPSII), photosystem II (PSII) maximum quantum efficiency (Fv/Fm), PSII photochemical efficiency in light (Fv'/Fm'), and electron transport rate (ETR), whereas the treatment decreased the intercellular carbon dioxide concentration (Ci), non-photochemical quenching coefficient (NPQ) and minimum fluorescence (Fo). Therefore, the SA application helps improve the photosynthetic efficiency and reduce photodamage to Z. armatum in the event of waterlogging stress. - COLLAPSE
    31 August 2023
  • Research Article

    Effects of 1-Naphthaleneacetic Acid on the Panicle and Fruit Characteristics of ‘Irwin’ Mango Trees
    Sun Woo Chung, Yeon Jin Jang, Chan Kyu Lim, Seolah Kim, Seong Choel Kim
    Excessive vegetative growth of mangoes inhibits reproductive growth during greenhouse cultivation, resulting in a loss of fruit production. To investigate the effect … + READ MORE
    Excessive vegetative growth of mangoes inhibits reproductive growth during greenhouse cultivation, resulting in a loss of fruit production. To investigate the effect of a 1-naphthaleneacetic acid (NAA) treatment, two concentrations (30 and 70 mg·L-1) were applied to the shoot apices of ‘Irwin’ mango cultivars. With changes in greenhouse temperatures, the characteristics of the reproductive organs were determined at BBCH (Biologische, Bundesanstalt, Bundessortenamt, and Chemische Industrie) scales of 615 and 809. During flower bud initiation, the maximum temperature reached 35°C, and the minimum temperature dropped to 5°C; the daily difference ranged from 1.3 to 23.9°C. The NAA treatment induced increased panicle emergence rates and reduced panicle malformation. In the flowering pattern, the flowering time was reduced, especially with 70 mg·L-1 NAA. The relative panicle quality index indicated that trees treated with 30 mg·L-1 NAA had the highest value, followed by the control and those treated with 70 mg·L-1 NAA. Additionally, the NAA treatment significantly increased fruit diameters and weights compared to the control. However, firmness decreased after the NAA treatment. Overall, this study highlights the potential of a NAA treatment as a tool for improving the yield and quality of mango crops, particularly under challenging environmental conditions. - COLLAPSE
    31 August 2023
  • Research Article

    Effects of Starfish and Lacquer Tree By-product Fertilizer on Growth of Tomato Plants

    불가사리와 옻나무 비료의 시용이 토마토 생장에 미치는 영향 변화

    Jung Eun Bae, Hae Keun Yun, Kyung il Park, Sang Gon Suh

    배정은, 윤해근, 박경일, 서상곤

    This study was conducted for investigate the effect of Starfish and Lacquer Tree by-product Fertilizer on Growth of Tomato Plants. When starfish …

    본 연구에서는 불가사리 분쇄물과 옻나무 부산물 비료의 토마토 생육 증진에 관한 효과를 구명하고자 하였다. 토마토 정식 후 옻나무 10% 단일처리구와 불가사리 5%와 …

    + READ MORE
    This study was conducted for investigate the effect of Starfish and Lacquer Tree by-product Fertilizer on Growth of Tomato Plants. When starfish and lacquer fertilizers were applied to tomatoes, the results of the root survival rate after transplanting tomatoes showed 93% root survival rate both in the control group and 10% starfish treatment, whereas 100% survival rate from 10% lacquer treatment, and 5% starfish with 5% lacquer mix. After transplanting, plant height was 173.0cm in10% lacquer treatment, and 191.13cm in 5% starfish with 5% lacquer mix were significantly higher than those of 144.5cm in 10% starfish single treatment. Chlorophyll content was significantly higher at 1.90 mg/g in the 5% mixed treatment group of starfish and lacquer compared to those of 1.47 mg/g in control. Among dry matter, 10% of starfish and 10% of lacquer treatments showed 13.69 g, which was 1.73 times higher compared to 7.92 g in control. Calcium content was significantly higher at 14.68 mg/L in the 5% mixture of starfish and lacquer compared to 11.41 mg/L in control. The higher the calcium content and hardness during storage, the lower the blossom-end rot incidence. The hardness of the fruit was the best in the mixed treatment of 5% starfish and 5% lacquer, and the shelf life was maintained over 60% even on December 8th. The highest tomato weight and marketable yield showed in the mixed fertilizer of 5% starfish and 5% lacquer. Therefore, the mixed 5% starfish with 5% lacquer tree by-product fertilizer can be used to promote the production and to improve the quality of tomato fruits preferred in markets


    본 연구에서는 불가사리 분쇄물과 옻나무 부산물 비료의 토마토 생육 증진에 관한 효과를 구명하고자 하였다. 토마토 정식 후 옻나무 10% 단일처리구와 불가사리 5%와 옻나무 5% 혼합처리구에서는 100%의 활착율을 나타내었으며, 대조구와 불가사리 10% 단일처리구에서는 93%의 활착율을 나타내었다. 토마토의 초장은 옻나무 부산물 10% 처리구에는 173.0cm, 불가사리 분쇄물 5%와 옻나무 부산물 5% 혼합처리구에서 191.13cm로 불가사리 분쇄물 10% 단일처리구의 144.5cm보다 유의성 있게 길었다. 토마토 식물체의 엽록소 함량은 무처리구의 1.47mg/g에 비해 불가사리 분쇄물 5%와 옻나무 부산물 5%의 혼합처리구의 1.99mg/g으로 유의성 있게 높았다. 건물중에서도 대조구의 7.92g에 비해 불가사리 분쇄물 10%와 옻나무 부산물 10% 혼합처리구에서 13.69g으로 1.73배 높았다. 칼슘 함량은 대조구의 11.41mg/L에 비해 불가사리 분쇄물 5%와 옻나무 부산물 5%의 혼합처리구에서 14.68mg/L로 유의성 있게 높았다. 칼슘 함량과 경도가 높을수록 배꼽썩음병과 발생률이 유의성 있게 줄어들었다. 불가사리 분쇄물 5%와 옻나무 부산물 5%의 혼합처리구에서 과실의 경도가 가장 높았는데 12월 8일이 되어도 저장성이 60% 이상 유지되었다. 불가사리 분쇄물 5%와 옻나무 부산물 5%의 혼합처리구에서 토마토의 무게와 시장상품수량이 가장 높게 나타났다. 그러므로 5% 불가사리 분쇄물과 5% 옻나무 부산물의 혼합처리를 함으로써 토마토의 생산성을 증대하고 품질이 향상된 토마토를 생산할 수 있을 것이다.

    - COLLAPSE
    31 August 2023
  • Research Article

    Model-based Calculations of Ventilation Rate, Carbon Dioxide (CO2) Absorption Rate by Plants, and Amounts of Ventilated and Supplied CO2 for Tomato Hydroponic Greenhouse during a Winter Season

    모델식에 의한 겨울철 토마토 수경재배 온실의 환기율, 작물의 이산화탄소(CO2) 흡수율, 환기에 의한 CO2 손실량 및 공급량 계산

    Su-Hyun Choi, Young-Hoe Woo, Dong-Cheol Jang, Young-Ae Jeong, Dae-Hyun Kim, Ho-Seok Seo, Eun-Young Choi

    최수현, 우영회, 장동철, 정영애, 김대현, 서호석, 최은영

    This study aimed to calculate ventilated carbon dioxide (CO2) and CO2 absorption by plants in a carbon dioxide (CO …

    본 연구는 모델식에 의한 겨울철 토마토 수경재배 온실의 환기율, 작물의 CO2 흡수율, 환기에 의한 CO2 손실량 및 공급량을 계산하고 …

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    This study aimed to calculate ventilated carbon dioxide (CO2) and CO2 absorption by plants in a carbon dioxide (CO2) supplemental greenhouse for tomato cultivation during the winter season using a model equation in order to predict the current CO2 concentration and next CO2 supply. A targeted CO2 concentration (600 μmol·mol-1) was supplied from 10 a.m. to 13 p.m. from January 18, 2022 to March 12, 2022 using a CO2 type of fertilizer operated with LPG (liquefied petroleum gas). The ventilation rate ranged from 9.66 to 26.49 m3·m-2·h-1 at greenhouse temperature of 16 to 34°C and relative humidity (RH) of 65 to 93%. The external temperature was in the range of 3 to 20°C and external RH was 42 to 95%. The calculated net assimilation rate was in the range of 5.6 to 11.1 g·m-2·h-1, and the amount of ventilated CO2 ranged from 1.35 to 3.88 g·m-2·h-1. When the CO2 concentration (Ci) was lower than the target CO2 concentration (Cg) at the starting time of CO2 fertilization, the calculated first CO2 supply was in the range of 17.79 to 28.18 g·m-2·h-1. When the CO2 concentration at a certain time (Ct) was lower than the targeted CO2 concentration (Cg) after t hours of supply, the next CO2 supply was between 3.0 and 32.6 g·m-2·h-1. The photosynthesis rate measured at the 83 DAT showed the maximum increase with the 600 µmol·mol-1 of CO2 supplement under the 400 µmol·m-2·s-1 of PPFD, whereas the 800 µmol·mol-1 of CO2 supplement results into its maximum increase under all the PPFD levels higher than 600 µmol·m-2·s-1. Integrating all of the results suggests that during the winter season given the low light intensity, a CO2 supply exceeding 800μmol·mol-1 does not proportionally increase the net assimilation rate; thus, the CO2 supply efficiency appears to be affected more by the ventilated CO2 loss than by the net assimilation rate. These model equations can be used to control the CO2 fertilization in order to improve the yields of fruit vegetables in greenhouses.


    본 연구는 모델식에 의한 겨울철 토마토 수경재배 온실의 환기율, 작물의 CO2 흡수율, 환기에 의한 CO2 손실량 및 공급량을 계산하고 현재 CO2 농도와 다음 CO2 공급량을 예측하고자 수행하였다. 폴리올레핀 필름으로 피복된 스마트 온실(면적 168m2)에서 2021년 11월 21일부터 2022년 3월 26일까지 실시하였다. 탄산시비는 LPG 연소형 탄산시비기로 CO2 600µmol·mol-1을 2022년 1월 18일부터 2022년 3월12일까지 오전 10시부터 13시까지 공급하였다. 온실 온도가 16–34°C, 상대습도(RH)가 65–93%, 온실 외부 온도가 3–20°C, 외부 RH가 42–95% 수준에서 순동화율은 5.64–11.09g·m-2·h-1, CO2 환기 손실량은 1.35–3.88g·m-2·h-1범위를 보였다. 탄산시비 개시 시점에서 온실 CO2 농도(Ci)가 목표 CO2 농도(Cg)보다 낮을 때 이를 고려하여 계산된 첫 CO2 공급량은 17.79–28.18 g·m-2·h-1 범위를 보였고 공급 t시간 후 온실 CO2 농도(Ct)를 계산하고 t시간 후 온실 CO2농도(Ct)가 목표 CO2농도(Cg)보다 낮을 때 계산된 다음 CO2 공급량은 3.0–32.6g·m-2·h-1범위를 보였다. 정식 후 83DAT에 PPFD 400µmol·m-2·s-1에서는 CO2 600µmol·mol-1일 때 광합성율이 최대 증가폭을 보였으며, PPFD 600 이상의 모든 광량에서는 CO2 800에서 최대 증가폭을 보였다. 본 연구 결과를 종합하여 볼 때 광량이 낮은 겨울 재배기에 CO2 800µmol·mol-1 이상을 투여해도 순동화율이 증가하지 않으며 CO2 공급 효율은 순동화율보다 환기에 의한 CO2 손실에 더 큰 영향을 받는 것으로 판단된다. 탄산시비를 위한 모델식은 CO2 공급량 조절에 활용될 수 있을 것으로 판단된다.

    - COLLAPSE
    31 August 2023
  • Research Article

    Effects of the Air Temperature and Root Zone Temperature in Greenhouses on the Early Growth of Tomatoes

    온실 내 대기온도와 근권부 온도가 토마토 초기생육에 미치는 영향

    Se Hun Ju, Beom Seon Lee, Eun Ji Kim, Yoon Go, Jin Sub Park, Haeyoung Na

    주세훈, 이범선, 김은지, 고윤, 박진섭, 나해영

    In this study, experiments were conducted to develop an efficient method of the early cultivation of tomatoes by implementing temperature control in …

    본 연구는 근권부 온도 조절을 통한 효율적인 토마토 초기재배법을 개발하기 위해 실험을 수행하였다. 온실 대기온도는 주간 20, 25, 30 및 35°C 야간 …

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    In this study, experiments were conducted to develop an efficient method of the early cultivation of tomatoes by implementing temperature control in the root zone. The greenhouse air temperature was maintained at 20, 25, 30, and 35°C during the daytime (12 hours) and at 18°C during the nighttime (12 hours). The experiment was conducted by setting the root zone temperature to 15, 20, 25, and 30°C for each air temperature. The growth of seedlings was found to be optimal when the root zone temperature was adjusted to 25 and 30°C for an air temperature of 30°C and the root zone temperature was adjusted to 20 and 25°C for an air temperature of 35°C. Regarding the root length, overall growth was favorable when the root zone temperature was adjusted to 20°C across all air temperature conditions. The leaf area and number of leaves showed the best growth when treated with a combination of an air temperature of 35°C and a root zone temperature of 25°C. The T/R ratio reached its high point at a root zone temperature 15°C due to the inhibited root growth. The compactness of tomato seedlings was high in the treatment with a root zone temperature of 20 in all air temperature treatment. The CGR (crop growth rate) and RGR (relative growth rate) were favorable at the root zone temperature of 20°C at all air temperature treatments. Based on the overall experimental results, it can be concluded that setting the root zone temperature to 20 or 25°C is effective for the early growth of tomatoes at air temperatures of 25, 30, and 35°C. The results of this study can help when regulating the early growth of crops in high and low air temperature conditions.


    본 연구는 근권부 온도 조절을 통한 효율적인 토마토 초기재배법을 개발하기 위해 실험을 수행하였다. 온실 대기온도는 주간 20, 25, 30 및 35°C 야간 18°C로 조절하였고, 근권부 온도는 각각의 대기온도에 15, 20, 25 및 30°C로 설정하여 실험을 진행하였다. 초장은 대기온도 30°C에 근권부 온도 25, 30°C, 그리고 대기온도 35°C에 근권부 온도 20, 25°C로 조절하였을 생육이 가장 좋았다. 근장은 모든 대기온도에서 근권부 온도를 20°C로 조절하였을 때 전체적으로 생육이 좋았다. 엽면적과 엽수는 대기온도 35°C와 근권부 온도 25°C를 조합처리 하였을 때 가장 높은 수치를 나타내었다. T/R율은 근권부 온도가 저온일 때 높았고, 묘 충실도는 근권부 온도 20°C 처리가 모든 대기온도 처리구에서 높게 나타났다. 작물생장율과 상대생장율도 모든 대기온도 처리구에서 근권부 온도 20°C 처리한 것이 가장 생장속도가 빠른 것으로 나타났다. 이상의 결과를 종합적으로 보았을 때 대기온도 25, 30, 35°C에서 근권부 온도를 20, 25°C로 설정하였을 때 토마토 초기 생육에 효과적이었다. 본 연구 결과는 고온기 및 저온기에 단기간 작물의 초기생육을 조절하는데 도움을 줄 뿐 아니라 식물공장과 같은 밀폐된 공간에서 에너지 절감형 재배 기술을 개발하는데 기초자료로 활용할 수 있을 것이다.

    - COLLAPSE
    31 August 2023
  • Research Article

    Seasonal Variations in Bioactive Compounds and Antioxidant Activity in the Leaves of Sasa quelpaertensis
    Min Hye Kang, Yu Mi Han, Yun Ji Lee, Yun-Jeong Ji, Hyun-A Seong, Hyung Don Kim
    Sasa quelpaertensis Nakai (SQ; Jeju-Joritdae) is an endemic species in Korea distributed on Jeju Island. This plant, which is typically used as … + READ MORE
    Sasa quelpaertensis Nakai (SQ; Jeju-Joritdae) is an endemic species in Korea distributed on Jeju Island. This plant, which is typically used as a type of herbal tea, has anti-inflammatory, anti-cancer, antioxidant, and anti-melanogenic effects. We investigated variations in the chemical compositions of the phenolic compounds and antioxidant activity of SQ leaves based on the harvest time and assessed its radical scavenging abilities using 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, while also assessing its ability to inhibit cellular reactive oxygen species (ROS). The levels of bioactive compounds and the antioxidant activity of Sasa quelpaertensis Nakai leaf water extract (SQW) differed by season. SQW collected in the spring, in which the total phenolic and flavonoid contents were highest, also contained the highest levels of vanillic acid and isoorientin (6-C-glycosylflavone) among the different extracts collected. This extract exhibited the second highest radical scavenging activity and the highest cellular ROS inhibitory ability in PC12 neuronal cells. Thus, spring is the most appropriate season for the collection of SQ leaves from the wild and for the harvesting of SQ leaves in the field for industrial use. SQ leaves harvested in the spring, with high levels of neuroprotective bioactive compounds, including vanillic acid and isoorientin, may be a promising material for the prevention of neurodegenerative diseases. - COLLAPSE
    31 August 2023
  • Research Article

    Seasonal Difference of Fruit Quality Attributes and Physiological Disorders in Paprika Cultivars under a Simulated Export System
    Si-Eun Byeon, Sangyo Jeong, Hnin Phyu Lwin, Jinhee Lee, Theint Thandar Latt, Hyowon Park, Yeo Eun Yun, Jung-Soo Lee, Jinwook Lee
    This study evaluated seasonal changes in various fruit quality attributes and the incidence of physiological disorders in the blocky and conical types … + READ MORE
    This study evaluated seasonal changes in various fruit quality attributes and the incidence of physiological disorders in the blocky and conical types of paprika cultivars using a simulated export system. The effects of three different pericarp colors were also investigated. ‘Scirocco’, ‘DSP 7054’, and ‘Volante’ cultivars were used as a blocky type, while ‘Raon red’, ‘Raon orange’, and ‘Raon yellow’ cultivars were used as a conical type with red, orange, and yellow pericarp color, respectively. The fruit was shipped at 10°C for two weeks, stored at 10°C for one week, and then transferred at 22°C for one week to simulate export by shipping. Storage temperatures and relative humidity levels greatly fluctuated in the winter season as compared to the summer season. Thus, fruit weight loss was higher in the winter season than in the summer season regardless of the cultivar type. However, the pericarp firmness was lower in the winter season. The soluble solids content and titratable acidity were higher in conical type than in the blocky type of paprika cultivar and highest in the ‘Raon red’ cultivar. The pericarp lightness and hue angle responded differently during the export season, depending on the pericarp color and fruit type. The incidence and severity of fruit shriveling were higher in the winter season than in the summer season, irrespective of the cultivar. Fruit shriveling was less severe in the conical type than in the blocky type, regardless of the pericarp color. Moreover, the incidence and severity of fruit softening, pedicel wilting, and pedicel browning were higher in the winter season than in the summer season. Therefore, the results indicate that the storage humidity levels likely play a pivotal role in the postharvest handling of paprika fruit quality during the seasonal export period. Thus, careful consideration of various techniques that are contingent upon the season of export and the specific type of fruit is required. - COLLAPSE
    31 August 2023
  • Research Article

    Optimization of the TRV-based Gene Silencing Efficiency and Growth Condition on Solanum lycopersicum var. ‘Hawaii7996’ Resistant to Bacterial Wilt Disease

    풋마름병 저항성 토마토의 TRV 기반 유전자 침묵 효율 검증 및 생육 조건 최적화

    Nayoung Kim, Won-Hee Kang, Ji-Su Kwon, Hyo-Jin Kim, Boseul Park, Seon-In Yeom

    김나영, 강원희, 권지수, 김효진, 박보슬, 염선인

    Ralstonia solanacearum (R. sol), a pathogen that causes wilt symptoms, is widely distributed in many regions. Breeding plants resistant against …

    Ralstonia solanacearum는 식물의 물관을 막아 시들음 증상을 유발하며 다양한 지역에 광범위하게 분포되어있어 전세계 토마토 생산량에 심각한 영향을 주는 병원균이다. 이를 해결하기 …

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    Ralstonia solanacearum (R. sol), a pathogen that causes wilt symptoms, is widely distributed in many regions. Breeding plants resistant against this pathogen is well known as the most effective control method. To this end, it is necessary to reveal the function of resistance genes, and virus- induced gene-silencing (VIGS) technology is one of the most widely used methods for analyzing gene functions. However, the problem with functional analysis of bacterial wilt resistance genes based on VIGS is the conflicting temperature environments of inoculated strains. Therefore, the objective of this study is to identify optimal plant size and temperature conditions for experiments using two srains considering a less complicated functional analysis of R. sol resistant candidate genes. We applied five different temperatures and then assessed the disease progress of R. sol inoculated tomatoes. The plants with the highest VIGS efficiency were those treated at 22°C for four weeks (4L), three weeks at a low temperature and one week at 30°C (3L1H), and two weeks at 22°C and two weeks at 30°C (2L2H). Also, we confirmed the most suitable temperature for identifying candidate genes against R. sol was found to be 22°C for plants grown for three weeks, followed by 30°C for one week. The results of this study can provide useful data to those seeking to establish a foundation for functional analysis research focusing on genes related to tomato bacterial wilt resistance.


    Ralstonia solanacearum는 식물의 물관을 막아 시들음 증상을 유발하며 다양한 지역에 광범위하게 분포되어있어 전세계 토마토 생산량에 심각한 영향을 주는 병원균이다. 이를 해결하기 위해 알려진 가장 효율적인 방법은 저항성 품종 육성으로 다양한 방법 중 하나인 바이러스 기반 유전자 침묵(Virus-Induced Gene Silencing, VIGS)기술을 이용하여 풋마름병에 대한 저항성 후보유전자의 발현을 억제 혹은 감소시켜 기능을 분석할 수 있다. VIGS를 기반으로 한 풋마름병 저항성 유전자 기능 분석 과정에서는 접종 균주의 온도 환경 차이가 상충된다는 문제점이 있다. 따라서 VIGS 적용 후 풋마름병원균 접종을 위한 최적 온도 조건과 이에 따른 식물 생장 조건을 검증하였다. 이를 위해 5가지 생육조건에 따른 VIGS 효율을 확인하고 이후 풋마름병원균 접종을 통해 토마토의 병 진전을 평가하였다. 그 결과, VIGS 효율이 높은 식물체는 4주간 저온(4L), 3주 저온 후 고온 1주(3L1H), 그리고 2주 저온 후 2주 고온(2L2H)을 처리한 식물체였으며 풋마름병을 접종하였을 때 저항성 대조구인 ‘Hawaii 7996’과 유사한 양상을 보이는 3주간 저온 후 1주간 고온 처리한 3L1H 개체가 풋마름병 저항성 후보유전자 발굴에 가장 효과적인 식물체임을 확인하였다. 이 연구 결과는 향후 토마토 풋마름병 저항성 관련 유전자의 기능 분석 연구 기반 구축에 유용한 데이터로 사용될 것이다.

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    31 August 2023
  • Research Article

    Analysis of Predictions of Garlic Bulb Weight using LASSO Regression Model
    Junki Kim, Hyungyong Lee, Tongjoo Suh
    As part of a preemptive response to ensure a stable supply and demand policy for garlic (Allium sativum L.), the Korean … + READ MORE
    As part of a preemptive response to ensure a stable supply and demand policy for garlic (Allium sativum L.), the Korean government strives to determine the final yield of garlic by observing its growth status by season. In particular, estimation of the bulb weight of garlic is one of the main steps in estimating garlic production, conducting observational work on spherical weight prediction is getting more important is getting more important. The observation data on garlic can be viewed as data for predicting the final weight, but since root growth occurs intensively at a specific time, there is a limit to predicting the final bulb weight only by the bulb weight corresponding to each observation time point. In addition, since the factors affecting the final bulb weight of garlic may be different for each growing stage, an estimation is required for each stage to predict the final bulb weight at each time point. In this study, garlic bulb weight estimation analysis, which reflects the characteristics of Korean garlic grown in an outfield, is performed using growth survey data. A stage-by-stage model for predicting the bulb weight of garlic at each time point is established, based on the fact that the factors influencing the bulb weight of garlic may differ according to the growth stage. In the process, LASSO regression is used for variable selection and coefficient estimation of the bulb weight of garlic. As a result of the estimation, significant factors regarding growth, lot and environment were identified stage by stage, providing indicators for forecasting final bulb weight of garlic at each stage. - COLLAPSE
    31 August 2023
  • Research Article

    Effect of Different Varieties, Processing Methods, Harvesting Times, and Storage Periods on the Quality of Maca
    Tingting Xu, Xiaoya Chen, Xiuming Cui, Chengxiao Wang, Lisha Qiu
    Maca, Lepidium meyenii Walpers (Brassicaceae), has been the subject of much research due to its unique biological activity. Maca is widely distributed … + READ MORE
    Maca, Lepidium meyenii Walpers (Brassicaceae), has been the subject of much research due to its unique biological activity. Maca is widely distributed in Yunnan Province, China, but is not well recognized in the market. In this study, through a chemical analysis, high-performance liquid chromatography (HPLC), and principal component analysis (PCA), the nutritional and bioactive components of different varieties, processing methods, harvesting times, and storage periods of maca were determined and analyzed. The results indicated that whole maca when dried at 35°C, harvested around the winter solstice and planted at an altitude of about 3,000 meters was favorable for the production of nutrients and bioactive components. Compared to black maca, yellow maca had higher levels of glucosinolates, flavonoids, alkaloids and macamides. In addition, a shorter storage period (less than one year) of maca leads to a higher content of bioactive components. The findings here provide a scientific basis for the cultivation and origin processing of maca. - COLLAPSE
    31 August 2023
  • Research Article

    Comparative Analysis between the ITS TaqMan SNP Genotyping Assay and CAPS for the Rapid Molecular Identification of Zoysia japonica and Zoysia sinica, Related Hybrid Lines, and the Habitat Distribution of Each Species

    한국에 자생하는 들잔디와 갯잔디, 교잡종의 신속한 식별을 위한 ITS TaqMan SNP genotyping assay와 CAPS의 비교 분석 및 각 종의 서식환경별 분포

    Dae-Hwa Yang, Ok-Cheol Jeong, Yu-Ryang Kim, Mi-Jeong Kang, Yang-Ji Kim, Ji-Hi Son, Seong-Seop Han, Mi-Young Park, Il-Doo Jin, In-Ja Song, Min-Ji Hong, Hyeon-Jin Sun, Hong-Gyu Kang, Hyo-Yeon Lee

    양대화, 정옥철, 김유량, 강미정, 김양지, 손지희, 한승섭, 박미영, 진일두, 송인자, 홍민지, 선현진, 강홍규, 이효연

    In this study, zoysiagrasses found in a major province of the Korean peninsula were collected and identified by molecular techniques. The zoysiagrasses …

    본 연구에서는 한국의 국립공원(산) 및 오름, 서해안 및 동해안, 남해안 부근의 해상국립공원을 포함한 해안 및 섬 등에서 한국잔디류 620점을 수집하였다. 이식, 보존 …

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    In this study, zoysiagrasses found in a major province of the Korean peninsula were collected and identified by molecular techniques. The zoysiagrasses used in this study were collected from mountainous national parks; ‘oreum’ areas; and from inland, coastal and island areas, including marine and coastal national parks on the west, east, and south coasts. Molecular identification was done by means of a cleaved amplified polymorphic sequence (CAPS) marker analysis based on the nrDNA-internal transcribed spacer (ITS) method established by our research team. Additionally, in this study the ITS-based TaqMan single-nucleotide polymorphism (SNP) genotyping assay was developed and used to perform molecular identification more rapidly and accurately. In total, 450 collected zoysiagrass plants were classified into three lines (234 Zoysia japonica plants, 62 Zoysia sinica plants, and 154 Z. japonica and Z. sinica hybrid plants) by molecular identification based on the ITS-based CAPS marker analysis and ITS-based TaqMan SNP genotyping assays. The results of the ITS-based TaqMan SNP genotyping assay were consistent with those of the ITS-based CAPS marker analysis. Both the ITS-based CAPS marker analysis and the ITS-based TaqMan SNP genotyping assay were found to be efficient molecular methods to identify the three species of zoysiagrasses. Specifically, the ITS-based TaqMan SNP genotyping assay method developed in this study produced results more rapidly compared to the ITS-based CAPS marker analysis method. The habitat distributions of each of the molecularly identified zoysiagrass plants investigated in this study were also assessed. The Z. japonica plants were mainly distributed in mountainous national parks and oreum areas as well as inland and coastal areas. The Z. sinica plants were distributed along the coast and on rocks and stones frequently submerged in seawater. The Z. japonica and Z. sinica hybrid plants were distributed more in the inland, coastal, and island areas than in the mountainous national parks and oreum areas. Findings related to the leaf blade width as a main external morphological classification characteristic of the molecularly identified 450 zoysiagrass plants are described below. The leaf blade width ranged from 4 to 5 mm for the Z. japonica plants, from 2 to 4 mm for the Z. sinica plants, and from 3 to 4 mm for the Z. japonica and Z. sinica hybrid plants. Additionally, some Z. japonica and Z. sinica plants and Z. japonica and Z. sinica hybrid plants with leaf blade widths of 2 to 3, 4 to 5, and from 2 to 3 and 4 to 5 mm, respectively, were also present. Because the ranges of the leaf blade width of each species overlapped, it was difficult to identify the hybrid lines using this metric. The feasibility of the developed molecular identification method for these three species of zoysiagrasses was confirmed in this study.


    본 연구에서는 한국의 국립공원(산) 및 오름, 서해안 및 동해안, 남해안 부근의 해상국립공원을 포함한 해안 및 섬 등에서 한국잔디류 620점을 수집하였다. 이식, 보존 및 증식 중인 다량의 잔디 450개체는 분자생물학적 분류동정을 수행하였으며, 들잔디(Zj) 234개체, 갯잔디(Zs) 62개체, 들잔디와 갯잔디의 교잡종(H) 154개체로 식별되었다. 본 연구에서 종의 분류 동정 결과는 새로 개발된 ITS 기반 TaqMan SNP genotyping assay 분석과 기존 방법인 ITS 기반 CAPS 분석에서 그 결과가 일치하였으며, ITS 기반의 TaqMan SNP genotyping assay 분석법은 ITS 기반 CAPS 마커 분석법보다 더 신속하게 종 식별 가능함이 확인되었다. 분류 동정된 450개체의 들잔디, 갯잔디, 교잡종의 서식환경별 분포를 조사한 결과, 들잔디의 경우 주로 국립공원(산) 및 오름, 내륙, 해안가 등에 분포되었고, 갯잔디의 경우 바닷물에 자주 잠기는 바위나 돌, 해안가의 바닷물이 인접한 부근에서 분포되었으며, 들잔디와 갯잔디의 교잡종은 국립공원 산 및 오름보다 내륙 및 해안가, 섬지역에 걸쳐 다양한 장소에 분포되었다. 기존 외부형태학적으로 주요한 분류 동정의 형질인 잎의 너비(엽폭)가 들잔디와 갯잔디 교잡종의 분류동정에 이용할 수 있는지 분자생물학적으로 분류 동정된 450 개체의 한국잔디류의 주요 외부형태적 분류 특성인 엽폭을 조사한 결과, 엽폭의 크기는 대체로 들잔디 4–5mm, 갯잔디 2–4mm, 들잔디와 갯잔디의 교잡종 3–4mm로 분석되었다. 일부 엽폭 크기는 들잔디(2–3mm)와 갯잔디(4–5mm), 들잔디와 갯잔디의 교잡종(2–3mm과 4–5mm)으로 확인되어 엽폭 크기에 의하여 교잡종을 확실히 분류 동정하기에 쉽지 않았기 때문에 본 연구에서 개발된 신속정확한 분자생물학적 분류 동정법의 필요성을 재확인하였다.

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    31 August 2023