About This Journal

Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provide scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.

 

2020 Impact Factor 0.869

 

  • Research Article

    Beneficial Elements Improve the Growth, Biomass Production, and Photosynthetic Pigments of Calendula officinalis L. Seedlings
    José Armando Hernández-Díaz, Jorge Jonathan Oswaldo Garza-García, Víctor García-Gaytán, Janet María León-Morales, Adalberto Zamudio-Ojeda, and Soledad García-Morales
    In this study, the effects of different concentrations of vanadium (V), selenium (Se), cerium (Ce), and iodine (I) on the growth parameters ... + READ MORE
    In this study, the effects of different concentrations of vanadium (V), selenium (Se), cerium (Ce), and iodine (I) on the growth parameters, biomass accumulation, and content of photosynthetic pigments during the seedling stage of marigold (Calendula officinalis L.) were evaluated. The beneficial elements V (3, 5, or 10 µM), Se (5, 10, or 20 µM), Ce (25, 50, or 100 µM), and I (5 or 10 µM) were applied once a week along with 50% Hoagland nutritive solution. Four applications were applied to the roots. Doses of 5 µM V and 50 µM Ce improved plant height, while 5 µM I promoted root growth. Leaf area and number of leaves increased with the application of 20 µM Se and 5 µM I, respectively. High concentrations of Se and Ce improved leaf dry weight, while 10 µM V increased the photosynthetic pigment content. The application of these elements contributed to the development and production of marigold seedlings during nursery growth. - COLLAPSE
    August 2021
  • Research Article

    Flowering Control of Elsholtzia angustifolia (Loes.) Kitag., a Short-Day Plant
    Nam Hyun Im, Hyeonjeong Kang, Jun Seo Mun, Hyo Beom Lee, Seong Kwang An, and Ki Sun Kim
    This study was conducted to investigate the photoperiodic flowering response of Elsholtzia angustifolia. Plants were grown under 9/15 h (day/night ... + READ MORE
    This study was conducted to investigate the photoperiodic flowering response of Elsholtzia angustifolia. Plants were grown under 9/15 h (day/night) (short day, SD), 16/8 h (long day, LD), and night interruption (NI, 4 h around midnight) conditions. Supplemental lighting with a light intensity of 4 µmol·m-2·s-1 was applied for the LD and NI treatments. Plants under continuous SD conditions developed the first floral bud at 5 weeks after sowing during the seedling stage and showed 100% flowering at 9 weeks. Under continuous LD and NI conditions, there was no flowering, and plants continued vegetative growth only. Early flowering under continuous SD conditions showed a small number of flowers. It was not desirable for marketable potted plant production, indicating that there is a need for a certain period of vegetative growth before flowering. Among plants under LD and NI conditions, transfer to SD conditions was performed at 3 or 6 weeks after transplanting (WAT) to manipulate the flowering time. The percent flowering was 100% when plants were transferred to SDs. On average, there were approximately 36 days to flowering from transfer to SD conditions at 3 and 6 WAT, and there was no significant difference between the photoperiod and transfer treatments. The number of inflorescences significantly increased as the transfer date was delayed. The number of leaves and branches was also significantly increased by the delayed transfer date. These results indicate that E. angustifolia is an obligate SD plant and that non-forcing conditions, such as LD or NI, effectively inhibit early flowering. - COLLAPSE
    August 2021
  • Research Article

    Identification of Dominant Microbial Community and Diversity in Continuously Cropped Pepper Fields
    Hui Wang, Li Liu, Shuyi Yu, Tianshu Guan, Baihong Li, Qi Wang, and Changyuan Liu
    Pepper blight is the most significant soil-borne disease affecting the continuous cropping of peppers. To identify the effect of Phytophthora capsici infection ... + READ MORE
    Pepper blight is the most significant soil-borne disease affecting the continuous cropping of peppers. To identify the effect of Phytophthora capsici infection on microbial flora, we isolated and counted the microorganisms collected from the rhizosphere soil of P. capsici-affected farms that continuously cropped pepper for 3, 6, and 9 years in Liaoning Province, China. The colony and cell morphology, physiological and biochemical characteristics, and 16S rDNA sequence of bacteria and actinomycetes were documented. In addition, colony and microscopic morphology of fungi and the rDNA-ITS sequence were analysed for classification. We observed that healthy and diseased peppers had the largest number of bacteria in the rhizosphere followed by actinomycetes and fungi. After infection, the number of bacteria and actinomycetes decreased with a corresponding increase in the number of fungi, leading to a reduction in the ratio of bacteria/fungi to actinomycetes/fungi. We identified 15 dominant bacterial strains, of which Bacillus represented the most abundant genus consisting of 7 strains followed by Flavobacterium and Staphylococcus. Furthermore, 15 of the 17 actinomycetes strains belonged to the genus Streptomyces. Among the six fungal strains, we found P. infestans, Fusarium, and Penicillium consisting of two strains each. This study elucidated the impact of pathogenic P. capsici on the composition of soil microbes over time and characterized several cultivatable dominant bacterial groups, which can provide a basis for practical intervention strategies to improve soil conditions for continuous cropping. - COLLAPSE
    August 2021
  • Research Article

    Effects of Irrigation Amount on Fruiting Period and EC Level by Growth Period on Growth and Quality of Melon (Cucumis melo L.) Using Coir Substrate Hydroponics During Autumn Cultivation

    멜론(Cucumis melo L.) 가을 재배 시 코이어 배지경에서 착과기 급액량과 생육 시기별 급액 EC수준이 생육과 품질에 미치는 영향

    Mi Young Lim, Su Hyun Choi, Gyeong Lee Choi, So Hui Kim, and Ho Jeong Jeong

    임미영, 최수현, 최경이, 김소희, 정호정

    This study was performed to identify the effects of changes in irrigation amounts on the ‘fruiting period’ and of irrigation electrical conductivity ...

    코이어 배지를 이용한 가을재배 멜론 수경재배 시 ‘착과 시기’ 급액량과 생육 시기 별 급액 EC 수준의 변화가 생육과 과실 특성에 미치는 영향을 ...

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    This study was performed to identify the effects of changes in irrigation amounts on the ‘fruiting period’ and of irrigation electrical conductivity (EC) level by the growth period on the growth and fruit properties of melon in hydroponics, using coir substrate. The irrigation amount was regulated based on the drainage rate (%), which was 30% after transplanting. The irrigation amount during the ‘fruiting period’ was regulated to the drainage rate of 10% and 30% for the two consecutive treatments of the irrigation level, and after 40 days of fruiting, the drainage rate was maintained at an equal level of 10%. The irrigation EC level was set to three treatments; low level (1.0-1.5-1.8 dS·m-1), middle level (1.5-1.8-2.0 dS·m-1), and high level (1.8-1.8-2.3 dS·m-1), according to the three growth periods: flowering period, fruiting period, and 40 days after fruiting. For the changes in drainage EC, a low irrigation EC level indicated a low drainage EC level, while a lower value was shown by the 30% drainage treatment with a higher irrigation amount than by the 10% drainage treatment. The results show that leaf length, leaf width, and leaf area were the highest at 30% drainage and the irrigation EC level on the fruiting period was high, but the middle level EC showed a similar pattern without a significant difference. Thus, for the EC level, the middle level (1.5-1.8-2.0 dS·m-1) appears to be most suitable. The irrigation amount had more influence on fruit weight, fruit height, fruit width, and fruit shape than the EC level. Meanwhile, the 30% drainage rate group showed higher values. An increase in the irrigation EC level by growth period was found to have increased the fresh and dry weights of the plant, although the fruit sugar content showed no significant difference. The findings of this study suggest that for the hydroponics of the net melon ‘PMR Worldstar’ using coir substrate, a 30% drainage rate was more favorable for melon fruit growth and quality for the irrigation level of the ‘fruiting period’ and with the irrigation EC level of 1.5-1.8-2.0 dS·m-1 during each growth period.


    코이어 배지를 이용한 가을재배 멜론 수경재배 시 ‘착과 시기’ 급액량과 생육 시기 별 급액 EC 수준의 변화가 생육과 과실 특성에 미치는 영향을 알아보기 위하여 본 실험을 수행하였다. 배액률(%)을 기준으로 급액량을 조절하였다. 정식 후 배액률 30% 수준으로 동일하게 급액을 하였고, ‘착과 시기’ 급액량을 배액률 10%, 30%로 조절하여 관수량을 2처리 하였다. 과실 착과 40일 이후 배액률은 10%로 동일하게 유지하였다. 급액 EC 수준은 생육 단계를 ‘개화기 - 착과기 - 착과 후 40일’ 3단계로 나누어 저수준(1.0-1.5-1.8dS·m-1), 중간 수준(1.5-1.8-2.0dS·m-1), 고수준(1.8-1.8-2.3dS·m-1)으로 3처리 하였다. 배액 EC 변화는 급액 EC 수준이 낮을수록 배액 EC 수준이 낮았고, 배액률 10% 처리구보다 급액량이 많은 배액률 30% 처리구에서 배액 EC 수준이 낮은 값을 나타내었다. 생육 조사 결과 엽장, 엽폭, 엽면적은 ‘착과 시기’ 배액률 30%, 급액 EC 고수준(1.8-1.8-2.3dS·m-1)에서 가장 높은 값을 보였으나, 중간 수준 EC도 유의적인 차이가 없이 높은 값을 보였다. 따라서 EC 농도는 중간 수준(1.5-1.8-2.0dS·m-1)이 적합한 것으로 판단된다. 과중, 과고, 과폭, 및 과형 지수 등은 EC 처리보다 배액률 처리의 영향이 더 높았으며 배액률 30% 처리구에서 높은 값을 나타내었다. 생육 시기 별 급액 EC 수준이 높을수록 식물체의 생체중과 건물중이 높았으나 과실 당도는 급액 EC 수준보다 배액률에 따른 공급량이 영향을 더 많이 받았으며 급액 EC 수준에 따른 유의한 차이가 나타나지 않았다. 본 연구 결과, 코이어 배지를 이용한 네트 멜론 품종 ‘피엠알월드스타’ 수경재배 시 ‘착과 시기’ 관수량은 배액률 30%로 설정하고, 생육 시기에 따른 급액 EC 수준은 ‘개화기 - 착과기 - 착과 후 40일’에서 1.5-1.8-2.0dS·m-1 공급 시 멜론의 생육과 과실 품질에 유리할 것으로 판단된다.

    - COLLAPSE
    August 2021
  • Research Article

    Development of Antimicrobial Paper with Ag Nanoparticles Synthesized Using Rubus coreanus Leaf Extract to Extend the Storage Period for Strawberry
    Jong Hyuk Kim, Ju Hee Nam, Min Ju Lee, Ho Jeong Jeong, and Il Rae Rho
    This study developed an antimicrobial paper against major putrefactive pathogens in strawberry through integrating Rubus coreanus leaf extract and silver nitrate (AgNO ... + READ MORE
    This study developed an antimicrobial paper against major putrefactive pathogens in strawberry through integrating Rubus coreanus leaf extract and silver nitrate (AgNO3). The R. coreanus extract and silver nitrate were synthesized and then assayed by spectrophotometry. A peak was found around 400 nm, the wavelength of silver particle absorption. Synthesis of R. coreanus extract-silver nitrate was confirmed by transmission electron microscopy (TEM). It was found that R. coreanus extract was combined with round nanosilver particles 100 nm or smaller, along with some triangular, polygon, and stick forms. Nanosilver synthesis was developed using the R. coreanus extract as a reducing agent and then the developed antimicrobial paper was investigated by scanning electron microscope (SEM). Nanosilver particles of nanometer level were also found in the antimicrobial paper. Ag was found to be evenly distributed through energy dispersive x-ray spectroscopy (EDS) analysis, and R. coreanus extract was also contained in the antimicrobial paper. The developed antimicrobial paper showed antimicrobial effects against all three putrefactive pathogens: Botrytis cinerea, Alternaria tenuissima, and Rhizopus stolonifer. Compared with general paper, the antimicrobial paper also minimally changed postharvest strawberry weight and hardness, helping to maintain fruit quality and lengthen the storage period. - COLLAPSE
    August 2021
  • Research Article

    Detailed Morphological and Molecular Characterizations of Melon (Cucumis melo L.) Accessions Collected from Northern Cyprus and Turkey
    Nihat Yılmaz, Nadide Kaya, Hasan Pınar, Fatih Hancı, and Aydın Uzun
    Information on the genetic diversity of local melon (Cucumis melo L.) genotypes is important for breeding and germplasm conservation efforts. This study ... + READ MORE
    Information on the genetic diversity of local melon (Cucumis melo L.) genotypes is important for breeding and germplasm conservation efforts. This study was to characterize the morphological and molecular features of local melon (Cucumis melo L.) accessions collected from different regions of northern Cyprus and Turkey. We studied 32 melon accessions, comprising 19 accessions collected from different areas in northern Cyprus, 4 hybrid cultivars, and 9 local accessions grown in Turkey. Their molecular characteristics were determined using sequence-related amplified polymorphism (SRAP) markers, and their morphological characteristics were determined according to criteria from the International Union for the Protection of New Varieties of Plants and Institute of Plant Genetic Resources. Our morphology data classified the 32 melon accessions into two distinct groups, and our molecular data identified 56 total bands were obtained using 11 SRAP primer combinations, and 11 of them were polymorphic at molecular characterization. The polymorphism rate was 0.69-0.96%. - COLLAPSE
    August 2021
  • Research Article

    Identification and Characterization of MADS-box Genes Involved in Floral Organ Development in Chinese Chestnut (Castanea mollissima Blume)
    Yang Liu, Guosong Chen, Yuerong Gao, Kefeng Fang, Qing Zhang, Qingqin Cao, Ling Qin, Yu Xing, and Shuchai Su
    Chinese chestnut (Castanea mollissima) is a woody plant species with important economic and ecological value and is widely cultivated for nut production ... + READ MORE
    Chinese chestnut (Castanea mollissima) is a woody plant species with important economic and ecological value and is widely cultivated for nut production. Due to its unique flowering structure, the identification of genes related to flower development is extremely important in Chinese chestnut, and MADS-box genes are directly involved in floral organ morphogenesis. In this study, we report a MADS-box gene family in Chinese chestnut. According to their gene structure and phylogenetic trees, 53 CmMADS proteins were identified and clustered into five groups in Chinese chestnut. The MIKCC group was further divided into 12 subgroups, and the BS subgroup was not found in Chinese chestnut. The classification and motif composition of Chinese chestnut MADS proteins were also analyzed. Additionally, we evaluated the expression of all the MADS-box genes in floral organs by quantitative real-time PCR. Overall, most of the type II genes had higher expression levels in the floral organs compared with leaves and cotyledons. These results provide valuable information for future studies of the regulation of MADS-box genes in Chinese chestnut during flower development. - COLLAPSE
    August 2021
  • Research Article

    High-frequency Microtuberization by One-step Culture without Medium Renewal from Leaf Explants of Pinellia ternata, a Medicinal Plant
    Jeong Ho Ahn and Seung Yeob Lee
    Pinellia ternata is an important medicinal herb in Northeast Asia, but its propagation rate is quite low in nature. This study was ... + READ MORE
    Pinellia ternata is an important medicinal herb in Northeast Asia, but its propagation rate is quite low in nature. This study was performed to develop a simple and rapid micropropagation method for applying to a plant factory system. In the regeneration process for the sustainable production of in vitro leaf explants, callus induction and propagation from leaf explants of P. ternata were excellent in MS medium containing 2.0 mg·L-1 2,4-D and 0.2 mg·L-1 BA. The highest number of shoots (8.8 per callus) was regenerated from calluses cultured on a medium containing 2.0 mg·L-1 NAA and 0.2 mg·L-1 KIN. The callus proliferation with subsequent plant regeneration was achieved at the same time in regeneration medium, and the year-round production of in vitro leaf explants was possible in the regeneration cycle. The direct microtuberization from in vitro whole leaf explants was also achieved through the one-step culture without medium renewal. The highest frequency of microtuber formation (93.0%) as well as the highest number of microtubers per explant (6.1) was observed by one-step culture in MS medium containing 2.0 mg·L-1 NAA and 0.2 mg·L-1 BA. Thus, the direct microtuberization from in vitro leaf explants was successfully achieved by the one-step culture protocol without medium renewal from microtuber induction to harvest. The adequately dried microtubers were well grown like an artificial seed in the pot culture containing a commercial soil. Accordingly, the in vitro microtubers through one-step culture could be commercially used as seed tubers for a plant factory system. - COLLAPSE
    August 2021
  • Research Article

    Nerine Latent Virus Diagnosed in Hippeastrum Bulbs in Korea
    Eun Song Heo and Sun Hee Choi
    Amaryllis (Hippeastrum hybridum) bulbs at the dormant stage were used to investigate contagious virus retention. A DNA fragment containing 612 bp was ... + READ MORE
    Amaryllis (Hippeastrum hybridum) bulbs at the dormant stage were used to investigate contagious virus retention. A DNA fragment containing 612 bp was amplified by reverse-transcription polymerase chain reaction with nerine latent virus (NeLV)-specific primers from amaryllis bulbs and characterized by nucleotide sequencing. Nucleotide sequences of the partial region of the NeLV coat protein gene were determined, and the sequences shared 93.9% to 98.5% identity with other NeLV isolates derived from various Amaryllidaceae species. In the phylogenetic analysis, amaryllis isolates from ‘Alfresco’ and ‘Red Lion’ cultivars used in this study were grouped with other NeLV isolates and distinctively separated from other carlavirus species. In this study, NeLV was found in amaryllis bulbs from the two cultivars. - COLLAPSE
    August 2021
  • Research Article

    Detection of Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis in Korea

    국내 호접란의 Cymbidium mosaic virusOdontoglossum ringspot virus 감염 현황

    Da-Seul Jeong, Kwang Hee Hong, and Kyeung-Il Park

    정다슬, 홍광희, 박경일

    The primary purpose of this study was to examine the effects of virus infection in Phalaenopsis cultivated across the country. RT-PCR analysis ...

    이 연구는 국내에서 재배하는 호접란의 바이러스 감염정도를 조사하기 위해 수행되었다. CymMV, ORSV 두 바이러스를 검정하기 위해 RT-PCR 검사를 진행하였다. 45품종의 기내배양묘의 바이러스 ...

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    The primary purpose of this study was to examine the effects of virus infection in Phalaenopsis cultivated across the country. RT-PCR analysis was used to detect two major viruses in orchid: CymMV and ORSV. The virus infection rate of in vitro plantlets from 45 cultivars was 83.6%, and the rate of potted plants from 21 cultivars was 92.5%. For CymMV, single infection in in vitro plantlets was 34.3%, and for ORSV, it was 11.4%. In the case of potted plants, single infection with CymMV was 57.0%, and ORSV alone was not detected. For double infection with both viruses, in vitro plants accounted for 37.9% and potted plants for 35.5%. No infection with both viruses was found in in vitro plantlets with ‘V3’ imported from Taiwan. Plants infected with CymMV showed chlorotic and necrotic spots on leaves and color break in petals and sepals of flowers, and those infected with both CymMV and ORSV featured yellow striped or mosaic symptoms.


    이 연구는 국내에서 재배하는 호접란의 바이러스 감염정도를 조사하기 위해 수행되었다. CymMV, ORSV 두 바이러스를 검정하기 위해 RT-PCR 검사를 진행하였다. 45품종의 기내배양묘의 바이러스 감염률은 83.6%였으며 21품종의 분화묘의 감염률은 92.5%였다. 기내배양묘의 CymMV 단독감염률은 34.3%이고 ORSV 단독감염률은 11.4%이다. 분화묘의 경우, CymMV의 단독감염률은 57.0%였으나 ORSV 단독감염은 확인되지 않았다. 두 바이러스에 대한 복합감염률은 기내배양묘와 분화묘에서 각각 37.9%, 35.5%였다. 대만 수입 ‘V3’ 품종 기내배양묘에서는 두 바이러스에 대한 감염이 확인되지 않았다. CymMV 감염주는 잎의 백화와 괴저반점의 증상을 나타냈고 꽃잎과 꽃받침의 화색변이를 보였다. 그리고 CymMV와 ORSV의 중복감염주는 퇴록 줄무늬 또는 얼룩무늬 증상을 보였다.

    - COLLAPSE
    August 2021
  • Research Article

    Estimation of Garlic’s Bulb Weight at Harvest Using a Multi-level Model Based on Growth and Meteorological Data

    마늘의 생육자료와 기상자료 기반으로 다층모형을 이용한 수확기 구중 추정

    Seong Cheon Choi, Jun Ki Kim, and Hong Seok Seo

    최성천, 김준기, 서홍석

    There is a large fluctuation in the production and price of garlic (Allium sativum L.) because it is grown mainly in the ...

    마늘은 노지 작물로 기상에 따라 작황 변동성이 크기 때문에 생산량과 가격의 불안정성이 크다. 안정된 수급관리를 위하여 정확한 생산량 예측이 선제적으로 이루어져야 한다 ...

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    There is a large fluctuation in the production and price of garlic (Allium sativum L.) because it is grown mainly in the open field and is vulnerable to weather conditions. Accurate forecasting is needed to manage production. The purpose of this study was to improve the accuracy of production forecasting by developing a model that estimates the weight of garlic bulbs at harvest based on growth and meteorological data. For this study, a two-level multilevel model was used to reflect the hierarchical data characteristics consisting of individual unit data that represented the above-ground growth conditions at the end of April and the lot unit data that represented the meteorological conditions from the end of April to harvest time. It was determined that the weight of the garlic bulb increased as the growth of the sheath diameter and the plant height increased at the end of April. As for the meteorological factors, it was estimated that precipitation had negative effects on the garlic bulb weight, while the pre-harvest precipitation, duration of sunshine, evapotranspiration, and the optimal temperature days had positive effects on the weight.


    마늘은 노지 작물로 기상에 따라 작황 변동성이 크기 때문에 생산량과 가격의 불안정성이 크다. 안정된 수급관리를 위하여 정확한 생산량 예측이 선제적으로 이루어져야 한다. 본 연구는 구 비대 초기의 마늘 생육과 기상자료를 기반으로 구중 추정모형을 개발함으로써 생산량 예측의 정확도 개선을 도모하였다. 분석은 개체단위인 4월 하순 지상부 생육자료와 필지단위인 4월 하순에서 수확기까지 기상 추정자료로 구성된 위계적자료 특성을 반영하여 2-수준 다층모형을 활용하였다. 분석결과 4월 하순 엽초경, 초장의 생육이 좋을수록 생구 무게가 증가하는 것으로 나타났다. 기상은 강수량, 강우일수는 구중에 감소효과를, 수확직전 강수량, 일조시간, 증발산량, 최적기온일수는 증가효과가 나타나 마늘 최종 구중에 유의한 요인으로 확인되었다.

    - COLLAPSE
    August 2021
  • Research Article

    Functional Properties of Newly Bred ‘Summer Prince’ Apples

    신육성품종 썸머프린스 사과의 기능적 특성

    Eun-Ho Lee, Hye-Jin Park, In-Kyu Kang, and Young-Je Cho

    이은호, 박혜진, 강인규, 조영제

    Apples are one of the most commonly eaten fruits in the world. Therefore, we studied the functionality of apple, a natural raw ...

    본 연구에서는 국내에서 새롭게 육성한 썸머프린스 사과 품종을 소재로 선정하고, 유용성분인 페놀 함량을 측정하여 항산화 효과와 기능성을 확인하였다. 썸머프린스 사과의 껍질과 whole ...

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    Apples are one of the most commonly eaten fruits in the world. Therefore, we studied the functionality of apple, a natural raw material that does not have toxicity or sensitivity problems. In this study, ‘Summer Prince’ apple cultivar, newly bred in Korea, was selected as a material, and the experiment was conducted by controlling the total phenolic content (TPC) at various concentrations. The experiment was confirmed by measuring TPC, anti-oxidant activity, and physiological-related enzyme inhibition activity. The peel and whole flesh of ‘Summer Prince’ apples were extracted using water and ethanol, and each extract demonstrated relatively high levels of TPC at 6.83 and 11.11 g·kg-1, and 3.41 and 4.65 g·kg-1, respectively. In the peel and whole extracts, 60 and 50% ethanol was measured. The anti-oxidant activity was measured in 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), anti-oxidant protection factor (PF), and thiobarbituric acid reactive substance (TBARs) assays. Both ‘Summer Prince’ peel and whole extract showed significant effects, but DPPH and ABTS, which showed water-soluble anti-oxidant effects, had better effects than positive control. Therefore, ‘Summer Prince’ apple extracts possess substantial anti-oxidant properties, and physiological- related enzyme inhibition activity was expected. Since inhibitors of enzymes involved in diseases are important factors in terms of drug availability, the ‘Summer Prince’ peel and whole extracts at 50-200 µg·mL-1 concentration were used to investigate the inhibitory effect. The expression levels of enzyme inhibitions activities were decreased in a concentration-dependent manner when ‘Summer Prince’ extracts were treated. These results support the high potential for using these extracts in functional food and cosmetics applications, as the ‘Summer Prince’ apples were determined to have various anti-oxidation, anti-inflammation, anti-wrinkle, and anti-diabetic functions.


    본 연구에서는 국내에서 새롭게 육성한 썸머프린스 사과 품종을 소재로 선정하고, 유용성분인 페놀 함량을 측정하여 항산화 효과와 기능성을 확인하였다. 썸머프린스 사과의 껍질과 whole 사과는 물과 에탄올을 이용하여 추출하였으며, 추출물은 각각 6.83, 11.11g·kg-1, 3.41, 4.65g·kg-1에서 상대적으로 높은 수준의 페놀 함량을 나타내었다. 껍질과 whole의 에탄올 농도별 추출물에서는 각각 60, 50% 농도에서 가장 높은 함량을 나타내었으며, 항산화 활성은 DPPH, ABTS, PF와 TBARs로 측정하였다. 썸머프린스 껍질과 whole 추출물 모두 우수한 효과를 나타내었으며, 수용성 항산화 효과를 나타내는 실험인 DPPH와 ABTS는 대조군인 BHT보다 우수한 효과를 보였다. 따라서 ‘썸머프린스’ 사과 추출물은 노화 방지 효과를 가진다는 것을 확인하였다. Astringent 효과, 그리고 TYR, elastase, collagenase의 저해 효과를 통해 썸머프린스 사과가 피부 노화 방지에 대한 효능이 있다고 판단되었다. α-Glucosidase와 α-amylase 억제제는 당뇨병 환자의 식후 고혈당증을 조절하기 때문에 썸머프린스 사과의 효능을 확인하기 위해 측정하였으며, HAase와 XOase 억제제는 피부를 보호하고 노화를 방지하는 중요한 요소이기 때문에 페놀 함량을 50-200µg·mL-1로 조절하여 측정한 결과, 농도의존적으로 생리활성에 관여하는 효소들을 억제하였다. 위의 결과에 따라 썸머프린스 사과가 항산화 효과, 항염증, 주름개선, 항당뇨 등 다양한 기능성을 가지는 것을 확인하여 기능성 식품 및 화장품 소재로 사용가능성을 확인하였다.

    - COLLAPSE
    August 2021