About This Journal

Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provide scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.

 

2020 Impact Factor 0.869

 

  • Review

    Floral Morphology and Pollination Process of Red-fleshed Dragon Fruit (Hylocereus polyrhizus) Grown in an Open Field
    Joanna Lee Ying Cho and Phebe Ding
    Hylocereus polyrhizus, commonly known as red-fleshed dragon fruit, is an exotic fruit crop whose commercial fruit production depends to a great extent ... + READ MORE
    Hylocereus polyrhizus, commonly known as red-fleshed dragon fruit, is an exotic fruit crop whose commercial fruit production depends to a great extent on fruit weight, often with the grower’s intervention for optimal fruit production. The purpose of this study was to characterize the floral structure, the morphology of pollen and stigma, and the reproductive process involving pollen-stigma interaction and pollen tube growth in red-fleshed dragon fruit plant grown in an open field using light, scanning electron, and fluorescence microscopy. Flowers of H. polyrhizus typified the floral traits of Cactaceae, in which large and white-coloured flowers with nocturnal anthesis are accompanied by strong floral emission. The extension of floral anthesis also indicated mixed pollination syndromes of nocturnal and diurnal pollinators. Self-incompatibility of the plant is evidenced by spatial segregation of the sexual organs with approach herkogamy and dry-type stigma. Numerous stigma lobes positioned above the anthers create a large area that enhances a large amount of pollen deposition. The highly ornamented, echinate sculpture of the pollen plays crucial roles in the attachment and adhesion of pollen grains on the stigma, and the presence of pellicles on papillae surfaces are specialized adaptations for pollen tube growth in dry-type stigma. The pollen germinated two hours after pollination with pollen tube elongation underneath the papillae tissues. Infertile pollen grains or incompatible pollination can be observed at 1 day after pollination (DAP), as indicated by the collapsed or dehydrated pollen grains, whereas compatible pollen grains travelling downward through the style transmitting tissues were observed at 2 DAP. After pollination, most of the pollen tube took approximately 4 days to reach the ovary cavity and fertilized the ovules leading to eventual fruit set. - COLLAPSE
    June 2021
  • Research Article

    Assessment of Growing Media and Fertigation for Production of Root Pruning Splice-Grafted Cucumber Seedlings
    Hyeon Woo Jeong, Hye Ri Lee, Ju Yeon Kim, Gam Gon Kim, Chae In Na, and Seung Jae Hwang
    In the Republic of Korea, the root pruning splice-grafting method is commonly used on watermelon (Citrullus lanatus) and cucumber (Cucumis sativus) seedlings ... + READ MORE
    In the Republic of Korea, the root pruning splice-grafting method is commonly used on watermelon (Citrullus lanatus) and cucumber (Cucumis sativus) seedlings to produce grafted plants. The growing medium is an important factor for rooting a transplant. In this study, we investigated the shoot growth and root development of cucumber seedlings in different growing media. ‘Shindong’ cucumber seedlings as scions were grafted onto ‘Shingiwon’ bottle gourd (Lagenaria siceraria) seedlings as rootstocks using the root-pruning one-cotyledon splice-grafting method. The grafted seedlings were transplanted into five different media: commercial growing medium (CGM), rockwool (RW), LC grow foam (LC), RC grow foam (RC), and terra-plug (TP). We also investigated the effect of fertigation on plant growth in the different media. Root morphology was significantly influenced by all five media. The total root length, root volume, and root surface area were greatest in the CGM. However, inorganic media (RW, LC, and RC) resulted in low root growth. Fertigation increased root growth, especially in the CGM. Root growth affected the shoot growth of cucumber seedlings. The shoot growth of cucumber seedlings was greatest in the CGM and TP medium, which also had good root development. In conclusion, CGM with fertigation was advantageous for cucumber root development and growth, and TP showed promise for use as a growing medium in the seedling stage. - COLLAPSE
    June 2021
  • Research Article

    Seasonal Fluctuation of Freezing Tolerance and Soluble Sugar Content in Three Sweet Persimmon Cultivars
    Joohee Ok, Seung Heui Kim, Kyeong-Bok Ma, Daeil Kim, Heon Sang Jeong, and Hyunsuk Shin
    Sweet persimmon (Diospyros kaki Thunb.) exhibits insufficient freezing tolerance (FT) in comparison with other temperate fruit trees. Information about FT of sweet ... + READ MORE
    Sweet persimmon (Diospyros kaki Thunb.) exhibits insufficient freezing tolerance (FT) in comparison with other temperate fruit trees. Information about FT of sweet persimmon is scarce. In this study, FT was analyzed in three sweet persimmons cultivars, ‘Fuyu’, ‘Taishuu’, and ‘Romang’, using an electrolyte leakage method for two successive years (Year 1, November 2017 to April 2018; Year 2, November 2018 to April 2019). Current-year shoot samples from 8-year-old sweet persimmon trees of each cultivar were collected to analyze FT in an experimental orchard (Pear Research Station, Naju, Korea). Average daily air temperatures increasingly declined from November 2017, reaching the minimum in mid-January 2018. They then steadily increased until April 2018 (Year 1). Patterns of average daily air temperatures of Year 2 were similar to those of Year 1 except that they reached the minimum at the end of December 2018. FT of the three cultivars constantly increased during cold acclimation (from November to January), whereas FT decreased during deacclimation (from February to April) for both years. Shoot samples (from Year 2) of the most freezing-tolerant ‘Romang’ and the most freezing-susceptible ‘Taishuu’ were used for soluble sugar analysis with HPLC. In both ‘Taishuu’ and ‘Romang’, total soluble sugar content was the highest in December 2018 and started to decrease from January 2019 until April 2019. Fructose and glucose contents showed the same pattern as total soluble sugar. Meanwhile, sucrose and maltose of ‘Taishuu’ and ‘Romang’ were detected minimally throughout the whole experimental period. These results indicated that fluctuations of total soluble sugar, fructose, and glucose contents paralleled FT of the three cultivars (p < 0.001). - COLLAPSE
    June 2021
  • Research Article

    Accumulation of Cadmium and Lead in Four Cultivars of Radish (Raphanus sativus L.) during the Seedling Period
    Yang Gyu Ku, Sung-Ju Ahn, and Yeon-Ok Kim
    Cadmium (Cd) and lead (Pb) are toxic even in small quantities and decrease crop yields. They are easily absorbed by plant roots ... + READ MORE
    Cadmium (Cd) and lead (Pb) are toxic even in small quantities and decrease crop yields. They are easily absorbed by plant roots, and thus enter the food chain, risking human health. Radish (Raphanus sativus L.) is a widely consumed root vegetable in Korea. Therefore, it is important to screen for a radish cultivar that exhibits low Cd and Pb accumulation and elucidate the Cd and Pb tolerance mechanism. In this study, we determined the effect of Cd and Pb stress on germination and growth in four radish cultivars, ‘Gaulgeojang’ (AG), ‘Iseogaul’ (IG), ‘Chongryong’(CR), and ‘Supertogwang’ (ST). Furthermore, Cd and Pb concentrations, production of nonprotein thiols (NPTs), and hydrogen peroxide (H2O2) levels were investigated in four cultivars of radish seedlings under Cd and Pb treatments. The cultivars showed tolerance to Cd and Pb stress during germination and seedling growth in the following order: CR > ST > IG > AG. CR, a tolerant cultivar, accumulated less Cd in roots and less Pb in both roots and shoots than the other cultivars. In contrast, AG, a sensitive cultivar, exhibited greater Cd and Pb accumulation in roots than the other cultivars. Cd and Pb treatments significantly increased the NPTs in all four cultivars; the highest level was found in CR. Cd and Pb treatments also increased H2O2 levels in all cultivars; the highest and lowest levels were observed in AG and CR, respectively. These results indicate that the greater Cd and Pb tolerance of CR may be attributable to its higher potential to limit Cd and Pb accumulation and to form complexes of metal-binding ligands with Cd and Pb. The results of this study provide information for the selection of the safest of the four common radish cultivars for growth in Cd- and Pb-contaminated soils. - COLLAPSE
    June 2021
  • Research Article

    Reflective Film Mulching Before Harvest Promotes Coloration and Expression of Ripening-Related Genes in Peach Fruits

    수확 전 수관 하부 반사필름 처리에 의한 복숭아 과실의 착색 및 관련 유전자의 발현 촉진

    Dan Bi Lee, Gook Jin Lee, Young Jun You, Soon Young Ahn, and Hae Keun Yun

    이단비, 이국진, 유영준, 안순영, 윤해근

    Fruit appearance is the most important factor of fruit quality in peach (Prunus persica). In order to determine the promotional effects of ...

    복숭아의 외관은 과실의 품질을 결정하는데 중요한 요인이다. 반사필름에 의한 복숭아 과피의 착색 촉진 효과를 구명하기 위하여, 복숭아(Prunus persica, 품종: Taiko) 과실을 ...

    + READ MORE
    Fruit appearance is the most important factor of fruit quality in peach (Prunus persica). In order to determine the promotional effects of increased light in the canopy on peach fruit coloration, reflective film was applied under trees in the field 7 days before the harvest of ‘Taiko’ peach. We then investigated pigmentation of the peel, fruit traits like total soluble solids, titratable acidity, and hardness; the expression of genes related to fruit coloration and ripening in a peach fruits; and photosynthetically active radiation (PAR) inside the tree canopy treated with reflective film mulching. The color of fruit skins treated with reflective film mulching was improved significantly according to their Hunter value compared to untreated fruit. There was no significant change in total soluble solids, acid content, and hardness in fruits treated with reflective film mulching compared to control. Quantitative real-time PCR analysis revealed that transcript levels of anthocyanin biosynthetic related genes (F3H and CHS) were up-regulated; however, the expression of ethylene synthesis-related genes (ACO and ERS1) and ripening-related genes (PL and PME) was not enhanced by reflective film mulching. These results suggest that reflective film mulching improved light irradiance in the tree canopy, which promoted fruit skin coloration via the the induction of anthocyanin biosynthesis in the peel of peach.


    복숭아의 외관은 과실의 품질을 결정하는데 중요한 요인이다. 반사필름에 의한 복숭아 과피의 착색 촉진 효과를 구명하기 위하여, 복숭아(Prunus persica, 품종: Taiko) 과실을 수확 7일전에 과원의 수관 하부에 반사필름을 피복한 후, 수확한 과실의 착색, 가용성 고형물 함량(SSC), 적정산도(TA), 경도(N), 과실의 성숙 및 착색과 관련된 유전자의 발현량을 조사하였다. 반사필름이 처리된 수관 내부의 광투과량(광합성유효방사)이 증가하고, Hunter a값이 현저하게 상승되었다. 반사필름이 처리된 과실의 가용성 고형물 함량, 적정산도 및 경도에는 큰 변화가 없었다. 반사필름을 처리한 과실에서 안토시아닌 생합성과 관련된 유전자(CHS 및 F3H)의 발현은 유도되었으나, 에틸렌 합성에 관여하는 유전자(ACO 및 ERS1) 및 세포벽 연화에 연관된 유전자(PL 및 PME)의 발현은 증가하지 않았다. 이러한 결과는 복숭아 수체 하부의 반사필름 멀칭으로 수관 내부의 광환경을 개선하여 복숭아 과피에서 색소합성 관련 유전자의 발현을 유도하여 착색이 촉진되었음을 의미하는 것이다.

    - COLLAPSE
    June 2021
  • Research Article

    Air Anions Promote the Growth and Mineral Accumulation of Spinach (Spinacia oleracea) Cultivated in Greenhouses
    Joung-Il An, Sora Lee, and Myung-Min Oh
    Recently, the positive effects of air anions on the growth of leafy vegetables cultivated in closed plant production systems have been widely ... + READ MORE
    Recently, the positive effects of air anions on the growth of leafy vegetables cultivated in closed plant production systems have been widely reported, but there is limited research on the influence of air anions on plants in greenhouses. Therefore, this study was conducted to determine whether the application of air anions can stimulate various physiological processes, including the growth, photosynthesis, and mineral uptake of spinach grown in different seasons (spring and fall) in greenhouses. Spinach was treated daily with three concentrations of air anions (low [LA], 3.4 × 105 ion/cm3; moderate [MA], 5.2 × 105 ion/cm3; and high air anion [HA], 8.3 × 105 ion/cm3). After 27 days of HA treatment during spring, the fresh weights of treated shoots and roots were at least 26% higher than of control shoots and roots. In fall, the shoot and root fresh and dry weights and the leaf area were significantly higher by at least 50% under all air anion treatments than those under the control treatment. The analyses of the photosynthetic rate, transpiration rate, and stomatal conductance supported the growth results of spinach. Although air anions did not affect the mineral uptake by plants in the spring, the accumulation of all minerals was increased by at least 130% in the fall; Fe ions particularly increased by up to 198% compared with those in the control. The results of this study were consistent with the results of a previous study on a closed production system and demonstrated the possibility of promoting plant growth and increasing production by air anion treatment in a greenhouse. - COLLAPSE
    June 2021
  • Research Article

    Shelf Life Prediction of ‘Royal Gala’ Apples Based on Quality Attributes and Storage Temperature
    Mengke Cao, Dong Wang, Lingyu Qiu, Xiaolin Ren, and Huiling Ma
    Phenotypic changes caused by postharvest deterioration of the quality attributes of apples cause substantial economic losses. Thus, strategies for accurate prediction of ... + READ MORE
    Phenotypic changes caused by postharvest deterioration of the quality attributes of apples cause substantial economic losses. Thus, strategies for accurate prediction of the shelf life of apples is urgently needed. In each of the three consecutive years from 2016 to 2018, freshly harvested ‘Royal Gala’ apples were stored at 0, 5, 15, and 25°C, respectively. Subsequently, 11 quality attributes were measured at periodic intervals until the end of storage. To screen fewer and more useful indexes, three input datasets were considered: temperature, color value (L*, a*, b*, △E, and C*), weight loss, firmness, titratable acidity, soluble solids content, starch, and reducing ascorbic acid (D1). The key quality attributes were screened by sparse principal component analysis (SPCA) (D2) and correlation analysis (CA) (D3), using shelf life as the output layer of the artificial neural network based on the back propagation (BP ANN) model. The results showed that the correlation coefficients (r) of the predicted and measured shelf life for D1, D2, and D3 were 0.996, 0.997, and 0.993, respectively, while the mean relative errors were 0.071, 0.074, and 0.074, respectively. Meanwhile, the relative percent root mean square (RMS) values were 0.088, 0.092, and 0.112, respectively. The application of SPCA reduced the quality attributes for the input dataset from 12 to 6. Therefore, SPCA-BP ANN was shown to be a useful model for accurate prediction of the postharvest shelf life of ‘Royal Gala’ apples. - COLLAPSE
    June 2021
  • Research Article

    The Effect of Carbon Nanomaterials on Senescence of Cut Flowers in Carnation (Dianthus caryophyllus L.)
    Di Zhang, Xiaotong Chen, Jiangyuan Sheng, Yafei Zhang, Xiaohui Shen, and Li Ren
    Carbon nanomaterials (CNMs) have remarkable chemical, physical, electrical, and structural properties and favorable biocompatibility. This study used carnation (Dianthus caryophyllus L.) cut ... + READ MORE
    Carbon nanomaterials (CNMs) have remarkable chemical, physical, electrical, and structural properties and favorable biocompatibility. This study used carnation (Dianthus caryophyllus L.) cut flowers as a model to evaluate the protective effects of 3 kinds of CNMs (single-walled carbon nanotubes [SWCNT], graphene quantum dots [GQD], and fullerenes [C60]) on the antioxidant activity and senescence of plant cells. We found that 1 mg·L-1 C60 and 25 mg·L-1 GQD extended the vase life (VL) of carnation by approximately 10%. SWCNT cannot be absorbed and transported by plant vascular tissue, and higher concentrations of SWCNT can block vascular tissue, leading to decreased VL. Physiological tests have shown that theThe malondialdehyde (MDA) and hydroxyl radical (OH·) levels significantly decreased after the GQD and C60 treatments, and the main factors that cause cell damage changed from H2O2 to OH·. The in vitro Fenton reaction and 2,2-diphenyl- 1-picrylhydrazyl (DPPH) free radical scavenging assay indicated that both C60 and GQD may inhibit OH· generation by approximately 10% to 15%, and GQD had higher DPPH free radical scavenging activity. Accordingly, a suitable concentration of C60 and GQD can influence reactive oxygen species metabolism and downstream biological events, including the cell redox state, the antioxidant system, and membrane lipid peroxidation, effectively delaying senescence and abscission of plant tissue. - COLLAPSE
    June 2021
  • Research Article

    Inhibition of Ethylene Binding and Biosynthesis Maintains Fruit Quality of ‘Formosa’ Plums during Postharvest Storage
    Yong-Tae Kim, Suong Tuyet Thi Ha, Ikjo Chun, and Byung-Chun In
    This study was conducted to develop an ethylene inhibition method using aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) to improve postharvest quality and extend ... + READ MORE
    This study was conducted to develop an ethylene inhibition method using aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) to improve postharvest quality and extend the shelf life of plums (Prunus subg. Prunus ‘Formosa’). Plums were sprayed preharvest with 150 mg·L-1 AVG and postharvest with 1 µL·L-1 1-MCP for 24 h. The results revealed that the combined treatment with AVG and 1-MCP (AVG+1-MCP) effectively suppressed skin color change, flesh firmness reduction, decay, and weight loss of plum fruit during postharvest storage. The acidity ratio was also significantly maintained during storage in AVG+1-MCP treated groups. The transcript levels of ethylene biosynthesis genes (PsACS3, PsACS4, and PsACO1) showed the same pattern as the amount of ethylene produced in plums. AVG+1-MCP treatment significantly inhibited transcript levels of PsACS3, PsACS4, and PsACO1 and the reduction in expression of signaling genes (PsETR1, PsERS1, and PsCTR1), resulting in a longer shelf life compared to the untreated control plums. Inhibition of ethylene biosynthesis and binding effectively suppressed senescence and ripening of plum fruit that show a climacteric rise of ethylene synthesis and respiration. - COLLAPSE
    June 2021
  • Research Article

    Comparative Analysis of Pigment Biosynthesis Gene Expression Related to Enhanced Skin Color in ‘Mishima’ Apples during Fruit Development
    Hye Jeong Cho, A Reum Han, and Cheol Choi
    In apple (Malus domestica), red coloration is an attractive trait for consumers. The color of fruit skin is determined by the distribution ... + READ MORE
    In apple (Malus domestica), red coloration is an attractive trait for consumers. The color of fruit skin is determined by the distribution of carotenoids and anthocyanins in the skin tissue. ‘Fuji’ apples have red stripes over a yellow-green background, and ‘Mishima’, a ‘Fuji’ bud sport variety, has superior coloration compared to its parent. To investigate the genetic mechanism underlying the enhanced pigmentation in ‘Mishima’ fruit skin, we characterized the expression of pigment biosynthesis genes in ‘Fuji’ and ‘Mishima’ apples during fruit development. CIELAB data showed lower L* (relative lightness) and higher a*/b* ratio (relative redness) values in ‘Mishima’ apples, suggesting increased red pigmentation, which correlated with the levels of pigment biosynthesis gene expression. In ‘Mishima’, two carotenoid biosynthesis genes (MdZISO and MdLCY-ε) were upregulated at many stages of fruit development. Furthermore, enhanced red pigmentation was resulted from higher expression levels of three anthocyanin regulatory (MdMYB10, MdWD40, and MdGST) and three structural (MdCHS, MdCHI, and MdF3’H) genes, suggesting their positive interaction. These data further our understanding of the molecular basis of the enhanced skin color of ‘Fuji’ apple varieties. - COLLAPSE
    June 2021
  • Research Article

    Traits and Gene Expression Analysis of Early and Late Ripening Pollination Constant Non-Astringent Persimmon Fruits at Different Developmental Phases

    조・만생종 완전단감의 과실발달 단계별 형질특성 및 유전자 발현 분석

    Eun-Gyeong Kim, Gwang-Hwan Ahn, Girim Park, Beunggu Son, Youngwhan Choi, Jumsoon Kang, Youngjae Lee, Byeong Il Je, and Younghoon Park

    김은경, 안광환, 박기림, 손병구, 최영환, 강점순, 이용재, 제병일, 박영훈

    We analyzed the major fruit traits and transcriptomes between a late-ripening cultivar ‘Fuyu’ and an early-ripening cultivar ‘Soshu’ of pollination constant non-astringent ...

    완전단감 품종 중 만생종인 ‘부유’와 조생종인 ‘조추’의 과실 발육 단계 별 주요 형질과 과실 내 발현되는 전사체의 비교 분석을 통해 ...

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    We analyzed the major fruit traits and transcriptomes between a late-ripening cultivar ‘Fuyu’ and an early-ripening cultivar ‘Soshu’ of pollination constant non-astringent persimmon (Diospyros kaki Thunb.) at different developmental stages. Sugar concentration and fruit peel color were measured every 3 weeks, until 18 weeks after full bloom (WAB) for ‘Soshu’ and 24 WAB for ‘Fuyu’. For the sugar concentration in fruit, sucrose levels began to decline 9 weeks earlier in ‘Soshu’. Fruit peel coloring progressed faster in ‘Soshu’, and differences in color between the cultivars were most notable at 18 WAB. Transcriptome analysis was carried out for fruit flesh every 3 weeks from 9-18 WAB. Gene ontology term analysis of the transcriptome indicated that catalytic and transport genes involved in metabolism and growth in cells and cellular organelles were mainly expressed, while in KOG and KEGG analysis, the majority of expressed genes were related to signal transduction, nuclear structure, and starch and sucrose metabolism. Differentially expressed genes were analyzed from two sets of samples: set I, in which fruits sampled at 12, 15, and 18 WAB were compared as the treatment group to the sample collected at 9 WAB as the control for each cultivar; and set II, in which fruit samples of ‘Soshu’ were compared as the treatment group to ‘Fuyu’ as the control at each WAB. For the DEGs of set I, containing three comparison combinations, the number of up-regulated genes in the treatment sample was highest at 18 WAB in ‘Fuyu’ and at 15 WAB in ‘Soshu’. For the DEGs of set II, the number of down-regulated genes and the number of up-regulated genes were higher in ‘Soshu’ compared to ‘Fuyu’ at 12 WAB and 15 WAB, respectively. We then compared the expression patterns of fruit trait-related genes with the phenotypic changes in traits. Our results indicated that UGPase-1 and AGPase-1, involved in carbohydrate metabolism, and CRTISO and PDS, involved in carotenoid biosynthesis, may induce early fruit ripening in ‘Soshu’. In addition, the transcription factors (TFs) ERF3, which binds to the promoter of ethylene-responsive genes; the MADS-box TFs RIN, AGL, and FUL; and the NAC TF controlling climacteric-type fruit ripening were considered to play a key role in controlling fruit ripening in ‘Fuyu’ and ‘Soshu’.


    완전단감 품종 중 만생종인 ‘부유’와 조생종인 ‘조추’의 과실 발육 단계 별 주요 형질과 과실 내 발현되는 전사체의 비교 분석을 통해, 만생종과 조생종 간 숙기 조절 관련 유전자를 탐색하였다. 개화 후 6주부터 ‘조추’는 개화 후 18주까지, ‘부유’는 24주까지 과피색과 유리당 함량을 3주 간격으로 조사한 결과 ‘조추’의 과실 성숙이 ‘부유’보다 이른 시기에 시작됨을 보여 주었다. 색도는 ‘부유’에 비해 ‘조추’에서 과피 착색이 빠르게 진행되었고, 개화 후 18주에 두 품종 간 차이가 현저하게 드러났다. 과실 내 유리당 함량을 조사한 결과, ‘조추’의 서당 감소 시점이 ‘부유’에 비해 약 9주 빨랐다. 두 품종의 과육 전사체 분석은 개화 후 9주부터 18주까지 3주 간격으로 수행하였다. 전사체의 GO term 분석에서는 세포와 세포소기관에서 대사과정과 발육을 조절하는 촉매작용과 수송 관련 유전자, 그리고 KOG 분석과 KEGG pathway 분석에서는 신호전달과 핵산구조, 전분과 당 대사에 관한 pathway 유전자가 주를 이루었다. DEGs 분석은 각 품종의 개화 후 9주의 과육 샘플을 대조구로 하여 이후 18주까지 3주 간격의 샘플을 처리구로 비교한 DEGs set Ⅰ과 각 단계별로 ‘부유’를 대조구로 하고 ‘조추’를 처리구로 비교한 DEGs set Ⅱ로 분석하였다. DEGs set Ⅰ의 발현량을 분석한 결과, 3가지 비교조합 중에서 ‘부유’는 개화 후 18주에, ‘조추’는 15주에 대조구에 비해 처리구에서 up-regulation된 유전자의 개수가 가장 많았다. DEGs set Ⅱ에서는 ‘부유’에 비해 ‘조추’에서 개화 후 12주에 down-regulation되는 유전자가, 15주에는 up-regulation되는 유전자가 많았다. 과실 형질 관련 후보유전자의 발현량을 형질변화와 비교 분석한 결과, 탄수화물 대사에 관련된 UGPase-1AGPase-1, 카로티노이드 생합성 경로의 CRTISOPDS는 ‘조추’의 조기 과실 성숙을 유도하는 유전자로 추정되었다. Transcription factor(TF)로는 ethylene responsive gene의 promoter에 결합하는 ERF3ACS의 promoter에 결합하는 MADS-box TF인 RIN, AGL, FUL, 그리고 climacteric type의 성숙을 조절하는 NAC가 ‘부유’, ‘조추’ 단감의 성숙 조절에 핵심적 역할을 하는 것으로 추정되었다.

    - COLLAPSE
    June 2021
  • Research Article

    Genetic Mapping of green-stripe in Tomato
    Woon Cho, Eun Sol Kang, and Je Min Lee
    Tomato (Solanum lycopersicum) has a high level of genetic variation in fruit color, which is an important fruit quality trait considered by ... + READ MORE
    Tomato (Solanum lycopersicum) has a high level of genetic variation in fruit color, which is an important fruit quality trait considered by breeders and consumers. The fruit stripe color pattern is determined by the green stripe (gs) locus and is observed only in the peel tissue in tomato. Using two different F2 populations (351 and 97 individuals), we mapped gs to the long arm of chromosome 7 and found that it cosegregated with TOMATO AGAMOUS-LIKE 1 (TAGL1). Association analysis using a TAGL1-specific derived cleaved amplified polymorphic sequences (dCAPS) marker distinguished 118 non-green-striped and 11 green-striped tomato cultivars. The chlorophyll content of green-striped peels (GSPs) was higher than that of light-green peels (LGPs), and the carotenoid content of red peels (RPs) was higher than that of yellow-striped peels (YSPs). Although we did not find sequence variation in the promoter and coding regions of TAGL1 between non-striped and striped tomatoes, TAGL1 expression in the YSPs was lower than that in the RPs. Therefore, the differential expression of TAGL1 may contribute to fruit stripe patterning. Marker-assisted selection using the TAGL1- specific marker identified here will facilitate breeding of fruit stripe patterning in tomato. - COLLAPSE
    June 2021