About This Journal

Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provide scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.

 

2019 Impact Factor 0.695

 

  • Research Article

    Comparative Analysis of Protein Patterns and Fruit Size in Three Asian Pears with Different Fruit Maturity Periods
    Bok-Rye Lee, Yun-Ju Baek, Seung Gon Wi, Ung Yang, and Sang-Hyun Lee
    This study aimed to investigate the relationships among fruit size and protein metabolism and profiles during fruit development and ripening of three ... + READ MORE
    This study aimed to investigate the relationships among fruit size and protein metabolism and profiles during fruit development and ripening of three Asian pear (Pyrus pyrifolia Nakai) cultivars predominantly grown in Korea. The final fruit dry weight was higher in the late-maturing cultivar ‘Niitaka’ than in the early-maturing cultivars ‘Wonhwang’ and ‘Whangkeumbae’. After 87 days after full bloom (DAFB), the fruit amino acid content significantly decreased in ‘Niitaka’ and ‘Wonhwang’ pears, while they remained stable at lower levels in the ‘Whangkeumbae’ pears during fruit development and ripening. At the beginning of our sampling period (57 DAFB) the protein content was the highest in ‘Niitaka’, followed by ‘Wonhwang’ and ‘Whangkeumbae’. The fruit protein content gradually decreased to 117 DAFB for ‘Niitaka’ or 102 DAFB for ‘Wonhang’ and ‘Whangkeumbae’, and then maintained at the similar level to the last sampling date. These reductions in the protein content were consistent with the increases in the protease activity. A rapid increase in the protease activity was observed in the ‘Niitaka’ and ‘Wonhwang’ pears during the early stage of fruit development, whereas a gradual increase was observed in the ‘Whangkeumbae’ pears during the entire period analyzed. The protein pattern varied across the various fruit developmental stages, revealing up- and down-regulated proteins during fruit development as well as highly expressed proteins at the middle phase of the period evaluated. Our results indicate that protein degradation by proteases in the early stage of fruit development, which in turn determines the fruit amino acid content, may affect the final size of Asian pears. - COLLAPSE
    February 2021
  • Research Article

    Effects of Root Pruning on Adventitious Root Formation, Enzyme Activities, and Hormone Levels in Paeonia suffruticosa ‘Fengdanbai’ Seedlings
    Zheng Wang, Gege Su, Songlin He, Liyun Shi, Dan He, Wenqian Shang, and Dajuan Yang
    Root pruning during early root development can optimize the root structure of woody plants. To determine the effects of root pruning on ... + READ MORE
    Root pruning during early root development can optimize the root structure of woody plants. To determine the effects of root pruning on tree peony (Paeonia suffruticosa), seedlings of the tree peony cultivar ‘Fengdanbai’ were subjected to varying degrees or different styles of root pruning. Various indexes were analyzed, including the anatomical structure of the root, root vitality, the activity levels of antioxidant enzymes, and the contents of endogenous hormones and their ratios. Comprehensive morphological observation showed that pruning freshly germinated roots that are 3 cm long by 1/3 (i.e., removing 1 cm) yielded the greatest rooting rate (90%), lateral root initiation (2.34 per seedling), and subsequent growth. Analysis of physiological and biochemical indicators during the critical rooting period (3-5 days after root pruning) showed that the levels of superoxide dismutase and abscisic acid (ABA) were decreased, while the oxidase activities of peroxidase, polyphenol oxidase, and indole acetate oxidase and the contents of the endogenous hormones indole acetic acid (IAA), gibberellin (GA3), and zeatin (ZT) were increased. IAA plays a key role in the process of adventitious root formation and the ratios of IAA/GA3, IAA/ABA, and IAA/ZT reflect endogenous hormone homeostasis during adventitious root formation. IAA/ABA and IAA/ZT showed higher values in the pruned roots compared to the control roots, which favor adventitious root induction and elongation. These results provide a basis for the study of adventitious root formation in tree peony. - COLLAPSE
    February 2021
  • Research Article

    Influence of Nocturnal Supplemental Lighting and Different Irrigation Regimes on Vase Life and Vase Performance of the Hybrid Rose ‘Charming Black’
    Liyun Shi, Songlin He, Zheng Wang, and Wan Soon Kim

    HST_39_01_03_F0.jpg

    Preharvest conditions, including photoperiod and irrigation regime, affect the keeping quality of long stem roses (Rosa x hybrida). Rose plants of the ... + READ MORE
    Preharvest conditions, including photoperiod and irrigation regime, affect the keeping quality of long stem roses (Rosa x hybrida). Rose plants of the cultivar ‘Charming Black’ were subjected to four different treatments to investigate how nocturnal supplemental lighting and different irrigation regimes influence post-harvest performance. Vase life, ornamental quality, water balance, petal color, vacuolar pH, soluble sugar content, and soluble protein content were investigated in this study. Treatment with nocturnal supplemental lighting extended the vase life and increased the daily ornamental value in cut roses. In addition, flowers grown under nocturnal supplemental lighting and without irrigation had significantly improved flower color and ornamental value and the highest values for soluble sugar content and soluble protein content. Withholding irrigation under supplemental lighting promoted maintenance of the water balance, which prolonged the vase life of the cut flowers. This study illustrates that supplemental lighting is helpful in extending the vase life of cut roses and that a non-irrigation regime under supplemental lighting results in the most promising physiological and biochemical responses. - COLLAPSE
    February 2021
  • Research Article

    Influence of Shade Treatment on Plant Growth Characteristics and Spear Production in Five Asparagus (Asparagus officinalis L.) Cultivars
    Rayhan Ahmed Shawon, Seo Yeon Ha, Tae Heon Lee, Thi Luyen Cao, Ho Cheol Kim, Jong Hyang Bae, and Yang Gyu Ku
    The objective of this study was to investigate the influence of a shade treatment on plant growth characteristics, bud break, and spear ... + READ MORE
    The objective of this study was to investigate the influence of a shade treatment on plant growth characteristics, bud break, and spear number of five asparagus (Asparagus officinalis L.) cultivars (Apollo, Atlas, Grande, UC157, and Walker Deluxe) and determine the most appropriate cultivar for cultivation in a shaded rain-shelter house. For the experiment, two-month-old seedlings were grown in the rain-shelter house with 30% shade using polyethylene film on the roof of the rain-shelter house, whereas the control plants were grown in a rain-shelter house without the additional layer of polyethylene. The shade treatment significantly increased the plant height, shoot number, shoot number per week, and bud number, but did not affect the mean number of days to bud break. The number of spears on asparagus grown in the shade was significantly higher than in the control. Under the shade treatment, Walker Deluxe and UC157 showed significantly higher shoot number and shoot number per week than the other cultivars. Compared to other cultivars, Walker Deluxe produced the highest bud number. In the control condition, Grande produced a higher number of shoots, shoots per week, buds, and roots than in the shade treatment. However, the shade treatment increased the ratio of spear numbers to bud numbers of Grande, resulting in a higher number of spears. Walker Deluxe with shade treatment had higher shoots, shoots per week, buds, roots, and spears than the control. These results suggest that Grande is more suited for growth in a rain-shelter house without shade treatment but that Walker Deluxe is more appropriate for cultivating in a rain-shelter house with 30% shade.문초록 - COLLAPSE
    February 2021
  • Research Article

    Effects of Supplemental Lighting with High-Pressure Sodium or Plasma Lamps on Quality and Yield of Cut Roses

    고압나트륨 및 플라즈마 램프 보광이 절화 장미의 품질 및 수량에 미치는 영향

    Min Jung Lee, Hyo Sook Seo, Sang Yoon Min, Jinhee Lee, Suhyun Park, Jeong Bin Jeon, Jiseon Kim, and Wook Oh

    이민정, 서효숙, 민상윤, 이진희, 박수현, 전정빈, 김지선, 오 욱

    Used as supplemental lighting (SL) in protected flower growing systems, newly developed sulfur plasma lamps (PLS) may confer benefits in terms of ...

    이 연구는 절화 장미의 동계 보광 재배 시 새롭게 개발된 플라즈마(PLS) 램프와 관행의고압나트륨(HPS)이 장미의 생육 특성, 수량 및 품질에 ...

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    Used as supplemental lighting (SL) in protected flower growing systems, newly developed sulfur plasma lamps (PLS) may confer benefits in terms of growth, yield, and quality. This study compared the effects of SL with PLS versus conventional high-pressure sodium lamps (HPS) on the growth characteristics, yield, and quality of cut roses cultivated in winter. Between October 10, 2016 and March 23, 2017, standard cut rose (Rosa hybrida) cultivars ‘Aqua’ and ‘Brut’ were grown under PLS and HPS with a photosynthetic photon flux density (PPFD) of 120 µmol·m-2·s-1 for 14 hours (between 17:00 and 07:00). Rose plants were cultivated in a plastic greenhouse in Gyeongsan, Gyeongsangbuk-do. A control condition used HPS with PPFD at 10 µmol·m-2·s-1 to offset the effect of the photoperiod. Cut flowers were harvested twice a week from December to March, and their growth and flowering characteristics, yield and quality of cut flowers were measured at each harvest. PLS had more blue (B) light and less red (R) light and near-infrared light than HPS; green and far-red light (FR) levels were similar. Plants under SL with PLS therefore received a higher B:R ratio and lower R:FR ratio than those under HPS. SL with PLS and HPS improved almost all of the characteristics of ‘Aqua’ and ‘Brut’ compared with the control. In particular, SL with PLS in ‘Aqua’ increased cut flower length, fresh and dry weights, vase life, and the number of higher grade products than SL with HPS. These differences could be associated with the relatively low R:FR ratio of PLS, which may have increased stem length and leaf area, thus increasing photosynthesis and resulting in higher yield and quality of cut flowers, as well as a shorter crop period. Likewise, the relatively high B:R ratio of PLS may have promoted gas exchange through stomatal opening and increased photosynthesis, resulting in higher yield and quality. However, the effect of light source for SL on morphological characteristics such as leaf area and stem length may be somewhat cultivar-dependent because the effect of SL with PLS in ‘Brut’ was smaller than that in ‘Aqua’


    이 연구는 절화 장미의 동계 보광 재배 시 새롭게 개발된 플라즈마(PLS) 램프와 관행의고압나트륨(HPS)이 장미의 생육 특성, 수량 및 품질에 미치는 효과를 비교하기 위해 실시되었다. 이를 위해 경북 경산시 소재 플라스틱하우스에서 스탠다드 장미(Rosa hybrida) ‘Aqua’와 ‘Brut’를 대상으로 10월 10일부터 이듬해 3월 23일까지 PPFD 120µmol·m-2·s-1의 HPS와 PLS로 14시간(17:00부터 익일 07:00) 동안 보광을 실시하였고, 대조구는 광주기의 효과를 상쇄하기 위해 이미 온실에 설치되어 있었던 HPS (10µmol·m-2·s-1)로 같은 시간 동안 점등하였다. 절화 수확은 12월부터 3월까지 주당 2회 수행되었고, 생장 및 개화 특성, 절화 특성, 절화 수확량 및 절화 등급별 수확량을 조사하였다. 광원 특성에 있어서는 PLS가 HPS보다 청색광(B)이 많은 반면 적색광(R)과 근적외선(NIR)이 적었으며, 녹색광(G)과 원적색광(FR)은 비슷한 수준이었다. 이로 인해 PLS의 B:R은 PLS보다 더 높고, R:FR은 더 낮았다. 보광 효과에 있어서는 PLS 및 HPS 보광처리구에서 대조구보다 ‘Aqua’ 및 ‘Brut’의 거의 모든 특성이 향상되었다. 특히, ‘Aqua’에 있어서 PLS 보광처리구에서 절화장, 화고, 생체중, 건물중, 절화수명, 상등품 수량 등이 HPS 처리구보다 높은 수치를 나타내었다. 이러한 차이는 PLS의 상대적으로 낮은 R:FR이 절화장과 엽면적을 증가시켰고 이로 인해 광합성의 증가로 수량 및 품질 향상과 작기 단축이 가능했으며, 상대적으로 높은 높은 B:R도 기공 열기를 통해 가스교환을 촉진하여 광합성을 증대시켰기 때문에 생겼을 것으로 생각된다. 다만, ‘Brut’에서는 PLS의 효과가 크게 나타나지 않아 PLS의 보광 효과에 품종 간 차이가 있을 것으로 보인다.

    - COLLAPSE
    February 2021
  • Research Article

    Production Rate of Runner Plants in a Plant Factory with Artificial Lighting as Affected by Crown Diameter of Strawberry Propagules
    Seon Woo Park, Yurina Kwack, and Changhoo Chun
    Use of the appropriate propagule size can maximize the propagation rate of strawberry (Fragaria × ananassa) transplants in an autotrophic transplant production method ... + READ MORE
    Use of the appropriate propagule size can maximize the propagation rate of strawberry (Fragaria × ananassa) transplants in an autotrophic transplant production method, a novel propagation method in a plant factory with artificial lighting for transplant production. During 90 days after planting the initial propagules, the cumulative number of transplants produced from propagules with a 5 mm crown diameter (CD5) was greater than those produced from propagules with a 4 mm crown diameter (CD4) or a 6 mm crown diameter (CD6). Timescales of propagation cycles (required timescale to produce new propagules; the sum of the time from planting propagules to the emergence of runners and the time from the emergence of runners to the separation of runner plants) of first, second, and third runner plants in CD5 were 2.1 – 20.7% shorter than those in CD4 or CD6. In second and third runner plants, the time from planting propagules to emergence of runners increased as propagule crown diameter decreased; however, the time from emergence of runners to separation of a runner plant decreased as crown diameter decreased. The fluctuation of the timescales of propagation cycles with propagation generation in CD5 was smaller than those in CD4 and CD6. After separating runner plants from their propagules, the number of leaves in CD5 fluctuated less than those in CD4 and CD6. These results indicate that propagules having a 5 mm crown diameter and two leaves are appropriate for the autotrophic transplant production method of strawberry in a plant factory. - COLLAPSE
    February 2021
  • Research Article

    Impact of the Heating Greenhouse and Root-Zone prior to Sunrise on Water Uptake, Photosynthesis, Plant Growth and Yield for Tomato Rockwool Hydroponics

    토마토 암면배지경에서 조기가온에 의한 수분흡수, 광합성, 생육 및 수량

    Eunkyung Cho, Ilhwan Cho, Juseong Park, Kiyoung Choi, and Eunyoung Choi

    조은경, 조일환, 박주성, 최기영, 최은영

    This study aims to determine effects of heating greenhouse and root-zone prior to sunrise in winter season on water uptake, photosynthesis, plant ...

    본 연구는 적합한 지상부 조기가온 시간 구명과 근권 조기가온이 토마토 수분생리와 수량에 미치는 영향을 구명하기 위하여 수행하였다. 실험I에서 지상부 조기가온은 무처리(Control ...

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    This study aims to determine effects of heating greenhouse and root-zone prior to sunrise in winter season on water uptake, photosynthesis, plant growth and yield for tomato rockwool hydroponics. Greenhouse heating was controlled at 25°C prior to sunrise with 4-day intervals [Control: None heating, PH-30: 30min-heating (07:30–08:00), PH-60: 60min-heating (07:00–08:00), PH-90: 90min- heating (06:30–08:00)] with Sap Flow- and Integrated Solar Radiation-automated irrigation systems (SF, ISR) in experiment I. Root-zone heating was controlled at 19°C prior to sunrise (RPH-90) or at 13°C (Control) with ISR system in experiment II. The substrate temperature was increased to 4°C for both PH-30 and PH-60 treatments, and to 7°C for PH-90. In the SF system, the first irrigation started average 18, 26, 30 mins earlier under the PH-30, PH-60, and PH-90, respectively compared to the ISR system. The water uptake was higher as heating duration was longer. The photosynthetic rate under the PH-90 was higher than that in the PH-30. The degree of leaf area change was greater between the PH-30 and Control, followed by between the PH-60 and PH-90. The substrate temperature ranged from 13.3 to 19.1°C under the RPH-90 treatment, whereas it ranged from 11.0 to 17.2°C under the control in the Experiment II. The water use efficiency (WUE), leaf area, specific leaf area, and marketable yield was higher about 7.0, 10.3, 23.8, and 23.0%, respectively under the RPH-90, compared to the control.


    본 연구는 적합한 지상부 조기가온 시간 구명과 근권 조기가온이 토마토 수분생리와 수량에 미치는 영향을 구명하기 위하여 수행하였다. 실험I에서 지상부 조기가온은 무처리(Control) 또는 일출 30분전(PH-30: 07:30–08:00), 60분전(PH-60:07:00–08:00), 90분전(PH-90:06:30–08:00)에 25°C로 처리하였고 급액방식은 SF와 ISR로 제어하였다. 실험II에서 근권 조기가온은 야간 난방기 온도를 13.5°C로 설정하여 지상부만 난방하는 무처리(Control) 또는 근권 난방 온도를 19°C로 설정하여 오전 6시부터 7시 30분까지 일출 전 90분간(RPH-90) 처리하였고 급액은 ISR 방식으로 제어하였다. 지상부 조기가온 실험에서 근권 온도는 PH-30과 PH-60처리구에서 Control보다 4°C 높아졌고 PH-90 처리구는 7°C 높아졌다. 급액개시 시간은 SF 급액방식에서 ISR 방식 보다 PH-30, PH-60, PH-90에서 각각 평균 18, 26, 30분 빨랐다. PH-90에서 단위 누적 일사량 당 물관수액흐름속도가 가장 높았고 수분흡수량도 조기가온을 일찍 시작할 수록 높았다. 광합성속도는 PH-90 처리구에서 PH-30보다 활발하였으며 엽면적은 4일간 PH-30 처리 후 Control 처리와 비교하였을 때 상승폭이 가장 큰 것으로 나타났고 그 다음으로 PH-60과 PH-90 처리간 차이가 컸다. 근권 조기가온 실험에서 RPH-90 처리구에서 근권온도는 13.3–19.1°C, Control은 11.0–17.2°C 범위를 나타냈다. 수분이용효율은 RPH-90에서 Control에 비해 7.0%, 엽면적과 엽면적비는 RPH-90에서 각각 10.3, 23.8% 높았으며 상품과 수량도 23% 많았다.

    - COLLAPSE
    February 2021
  • Research Article

    Quality Change During MAP Storage of Strawberry (Fragaria × ananassa Duch.) After Forced-Air Cooling with Silicone Rubber Pads

    실리콘 고무 패드에서 차압 예냉 처리된 딸기(Fragaria × ananassa Duch.)의 MAP 저장하는 동안 품질변화

    Sae Jin Hong, Seong Jun Kim, Cheon Soon Jeong, and Hyang Lan Eum

    홍세진, 김성준, 정천순, 엄향란

    Forced air cooling is used to lower the temperature of strawberries harvested at high temperatures. During forced air cooling, the skin of ...

    외부 기온이 높은 시기에 수확한 딸기의 품온을 낮추기 위해서 실시하는 차압 예냉은 처리하는 동안에 딸기 과피와 용기바닥이 밀착되어 과피가 눌리는 현상이 발생된다 ...

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    Forced air cooling is used to lower the temperature of strawberries harvested at high temperatures. During forced air cooling, the skin of the strawberry comes into contact with the bottom of the container, causing the skin to become bruised. In this study, we fitted a pre-cooled silicone rubber pad into the forced air cooling container, and placed strawberries inside. Strawberries were then treated with modified atmosphere packaging (MAP), stored at 5°C, and changes in quality parameters were recorded. Strawberries placed inside a forced air container with a 50 N shore hardness degree (Sh) A silicone rubber pad maintained a better appearance than those in containers with 40 N Sh A or 60 N Sh A pads. Strawberries with the 50 N Sh A pad also displayed more uniform skin drying during forced air cooling and the epidermal tissues of the skin did not collapse. For long-term storage of strawberries harvested in May when the outside temperature rises, MAP treatment is required to prevent weight loss, with pre-cooling to lower the product temperature. For strawberries treated with MAP after forced air cooling using a 50 N Sh A silicone rubber pad, no effects on fruit firmness or soluble solid content were observed. However, this treatment is effective in retarding aging, delaying pigmentation and suppressing mold development.


    외부 기온이 높은 시기에 수확한 딸기의 품온을 낮추기 위해서 실시하는 차압 예냉은 처리하는 동안에 딸기 과피와 용기바닥이 밀착되어 과피가 눌리는 현상이 발생된다. 본 연구에서는 차압 예냉 용기에 적합한 패드를 선정하고, 선정된 실리콘 고무 패드를 이용하여 차압 예냉을 실시한 후 예냉처리 된 딸기를 MAP 포장처리를 하여 5°C에 저장하면서 품질변화를 확인하였다. 물성이 50N Sh A인 실리콘 고무 패드는 40N Sh A과 60N Sh A의 패드에 비해서 딸기의 외관이 우수하였으며, 차압 예냉을 처리하는 동안 균일한 표피건조에 효과적이었다. 그리고 실리콘 고무 패드와 딸기 접촉면의 표피조직은 매끈하고 물러지는 현상이 없었다. 외기온도가 올라가는 5월에 수확한 딸기의 장기 저장을 위해서는 품온을 낮추는 차압 예냉과 함께 수분 손실을 막기 위해서 MAP 처리가 요구된다. 물성 50N Sh A의 실리콘 고무 패드를 용기에 깐 후 차압 예냉을 처리하고, MAP 포장을 함께 실시하면, 딸기는 경도와 당도 등의 품질에는 영향을 미치지 않으며 과피 색의 변화를 최소화하고 곰팡이 발생을 억제시키면서 노화를 지연시키는데 효과가 있다고 판단된다.

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    February 2021
  • Research Article

    Phenotypic and Genetic Characterization of Three Different Types of Dangyooza (Citrus grandis), Korean Landrace Citrus
    Kyung Uk Yi, Kook Lhim Zhin, Eun Ui Oh, Sang Suk Kim, Ho Bang Kim, and Kwan Jeong Song
    Dangyooza (DY), a Korean landrace citrus similar to pomelo (Citrus grandis L. Osbeck), has been consumed as a fruit and used in ... + READ MORE
    Dangyooza (DY), a Korean landrace citrus similar to pomelo (Citrus grandis L. Osbeck), has been consumed as a fruit and used in folk remedies to treat colds for centuries, and it is still used as a garden tree. DY has two natural variants, the buk-daengyooza (BDY) and the seol-daengyooza (SDY) based on distinguishable phenotypic fruit characteristics. However, there is little scientific analysis available not only on the morphological and chemical properties but also on the genetic basis of these natural variants. To gain a better understanding of DY, several morphological, phytochemical, chromosomal, and genetic traits were analyzed in DY and its natural variants, BDY and SDY. Morphological characteristics such as leaf and fruit shape, fruit hardness, and peel thickness were used to discriminate SDY from DY and BDY. Notably, SDY produced smaller fruit with thinner peels than those of DY and BDY. The major flavanones occurring in citrus were also markedly higher in SDY than those of DY and BDY. However, chromosomal configuration and genetic diversity analysis using random amplified polymorphic DNA, simple sequence repeat, and plastid trnL/F barcoding markers were unable to clearly discriminate the phylogenetic relationships among the DY types. This suggests that SDY might have arisen from somatic mutation, perhaps as a nucellar seedling. - COLLAPSE
    February 2021
  • Research Article

    Genetic Kinship and Discrimination between the Cultivars Consisting a Sister Cultivar Group in Spring Orchid Cymbidium goeringii

    춘란(Cymbidium goeringii)의 자매품종 그룹내 품종들 간의 유전적 친족성과 식별에 대한 연구

    Da Eun Nam, Jin Seok Yu, Seung Woo Noh, Kyung Suk Lee, Jung Hee Hwang, Dae Gun Lee, and Ki Wha Chung

    남다은, 유진석, 노승우, 이경석, 황정희, 이대건, 정기화

    Cultivars collected from similar regions and exhibiting similar phenotypes to each other are commonly called sister cultivars in spring orchid (Cymbidium goeringii ...

    춘란(Cymbidium goeringii)에서 비슷한 지역에서 채취되고 유사한 표현형을 보이는 품종들을 서로 자매품종들로 흔히 불린다. 그런데, 자매품종들의 원 채집지나 채집자에 대한 정확하지 ...

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    Cultivars collected from similar regions and exhibiting similar phenotypes to each other are commonly called sister cultivars in spring orchid (Cymbidium goeringii). However, no exact information is available for the original collection places and collectors of sister cultivars, and no study has revealed the degree of genetic relationship among them. This study was performed to determine genetic kinship among sister cultivars. Thirteen flower-variant types of cultivars consisting of four sister cultivar groups (358 samples) were collected, and then thirteen combined genotypes were identified by the determination of 12 SSR genotypes using the multiplex PCR. The phylogenetic analysis and the sibling probabilities of the combined genotypes suggested that the cultivars constituting each group were close relatives. Some combined genotypes were regarded to be a cultivar-specific, but others were observed simultaneously in two or more cultivars within a group. Therefore, it was suggested to establish a new classification criteria on the sister cultivars. This study particularly proposes to prepare a detailed evaluation criteria for cultivar pairs of ‘Hongnoeul’ and ‘Ruby’, and ‘Noeul’ and ‘Sehongso’ in group-1 through exact co-analysis of the phenotypes and genotypes. As the first study on the genetic kinship between sister cultivars, this study will help to understand the genetic nature of the sister cultivars, and to re-classify them.


    춘란(Cymbidium goeringii)에서 비슷한 지역에서 채취되고 유사한 표현형을 보이는 품종들을 서로 자매품종들로 흔히 불린다. 그런데, 자매품종들의 원 채집지나 채집자에 대한 정확하지 않은 경우가 많고, 서로 간의 유전적 근연성을 밝힌 연구는 없었다. 본 연구는 자매품종들 간의 유전적 근친성에 대해 처음으로 조사하였다. 4 자매품종 그룹의 13 품종(358 시료)을 수집한 후 12 SSR의 유전자형을 multiplex PCR 방법으로 분석하여, 13 가지 복합유전자형을 분리하였다. 각 그룹에서 유래된 복합유전자형들의 계통도 분석과 형매 확률은 각 그룹을 구성하는 품종들이 서로 가까운 친척일 가능성을 시사하였다. 복합유전자형들은 품종-특이적인 경우도 있었지만, 일부는 그룹 내 여러 품종에서 동시에 관찰되었기 때문에, 자매 그룹내 품종들의 새로운 분류 기준의 확립이 요구되었다. 특히, 유사한 꽃의 특징을 보이면서 유전적 식별이 어려운 ‘홍노을’과 ‘루비’ 및 ‘노을’과 ‘세홍소’는 세밀한 표현형과 유전자형의 병행 분석을 통한 품종 별 정확한 식별 기준의 확립 및 품종 분류의 재정립이 필요하다고 판단된다. 본 연구는 춘란의 자매품종들의 근친 정도를 처음 밝힌 연구로서, 자매품종들의 유전적 본질에 대한 이해도를 높이고, 자매품종 명명의 재정립에 유용한 유전적 식별 기준을 마련하는데 도움을 줄 수 있을 것이다.

    - COLLAPSE
    February 2021
  • Research Article

    Genetic Relationship Analysis by using FT-IR Spectrum of Genomic DNA from Peach Cultivars Related to ‘Yumyeong’

    Genomic DNA의 FT-IR 스펙트럼을 이용한 복숭아 ‘유명’ 관련 품종의 유연관계 분석

    Seung-Yeob Song, Ju-Hyun Lee, Kidong Hwang, Namhee Jeong, In Myung Choi, and Jung Hyun Kwon

    송승엽, 이주현, 황기동, 정남희, 최인명, 권정현

    The genetic relationships of offspring cultivars from ‘Yumeyong’ peach were evaluated by using Fourier transform infrared (FT-IR) spectroscopy. Four offspring cultivars were ...

    본 연구는 FT-IR 스펙트럼 데이터를 기반으로 다변량통계 기법을 이용하여 ‘유명’ 복숭아 자손 품종의 유전적 관계를 분석하였다. ‘유명’과 ‘치요마루’의 교배를 통해 ...

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    The genetic relationships of offspring cultivars from ‘Yumeyong’ peach were evaluated by using Fourier transform infrared (FT-IR) spectroscopy. Four offspring cultivars were bred by controlled hybridization between ‘Yumyeong’ and ‘Chiyomaru’, 2 from open pollination, and 1 from bud sport mutation. The FT-IR spectrum of genomic DNA were analyzed by using principal component analysis (PCA), partial least square discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA). FT-IR spectra showed that typical spectral differences were existed in the frequency regions of guanine ring (C6 = C6), thymine ring (C4 = O4), cytosine, N-H stretching (amide I), C = O stretching vibrations (amide II), and PO2- ionized asymmetric and symmetric stretching, respectively. Among the offspring cultivars between ‘Yumyeong’ and ‘Chiyomaru’, ‘Mihong’ and ‘Misshong’ showed higher genetic similarity to ‘Yumyeong’ than ‘Chiyomaru’. On the other hand, ‘Yumi’ and ‘Soomee’ showed higher genetic similarity to ‘Chiyomaru’ than ‘Yumyeong’. ‘Wolmi’, a mutant cultivar from ‘Yumyeong’, showed close relationship with ‘Yumyeong’, however, the genetic similarity was lower compared to other crossbreeding cultivars, ‘Misshong’, and ‘Mihong’.


    본 연구는 FT-IR 스펙트럼 데이터를 기반으로 다변량통계 기법을 이용하여 ‘유명’ 복숭아 자손 품종의 유전적 관계를 분석하였다. ‘유명’과 ‘치요마루’의 교배를 통해 육성된 4품종, ‘유명’ 품종의 자연교잡실생 2품종 그리고 아조변이 1품종을 사용하였다. Genomic DNA의 FT-IR 스펙트럼 데이터로부터 principal component analysis(PCA), partial least square discriminant analysis (PLS-DA) 그리고 hierarchical clustering analysis(HCA) 분석을 실시하였다. IR 스펙트럼은 구아닌 고리(C6 = C6), 티민 고리(C4 = O4), 사이토신, N-H 스트레칭(amide I), C = O 스트레칭 진동(amide II) 및 PO2- 이온화 대칭과 비대칭 스트레칭의 주파수 영역에서 전형적인 스펙트럼 차이가 존재함을 나타낸다. ‘유명’과 ‘치요마루’의 교배 품종 중 ‘미홍’과 ‘미스홍’은 ‘치요마루’보다 ‘유명’에 유전적 유사성이 더 높았다. 반면 ‘유미’와 ‘수미’는 ‘유명’보다 ‘치요마루’와 유전적 유사성이 더 높았다. ‘유명’의 변이 품종 ‘월미’는 ‘유명’과 밀접한 관계를 보였지만, 다른 교배 품종인 ‘미홍’과 ‘미스홍’에 비해 유전적 유사성이 낮았다.

    - COLLAPSE
    February 2021
  • Cultivar and Germplasm Release

    New Korean Ginseng Cultivar ‘Cheonmyeong’ with High Yield and Rusty Root Tolerance
    Jang-Uk Kim, Young-Chang Kim, Dong-Hwi Kim, Jung-Woo Lee, Kyong-Hwan Bang, Dong-Yun Hyun, Myeong-Hwan Jang, Bong-Jae Seong, Gun-Hwan Park, Ye-ji Yoon, and Ick-Hyun Jo
    In 2008, a new Panax ginseng (ginseng) cultivar, ‘Cheonmyeong’, was bred by pure-line selection for tolerance to physiological disorders using a genetic ... + READ MORE
    In 2008, a new Panax ginseng (ginseng) cultivar, ‘Cheonmyeong’, was bred by pure-line selection for tolerance to physiological disorders using a genetic resource collected from the farm race field, Buyeo, Chungnam, Korea. In 2019, ‘Cheonmyeong’ was registered as a new breed by the Korea Seed and Varieties Service (grant number 7467) and accorded plant breeders’ rights. ‘Cheonmyeong’ has a yellowish orange pericarp, which has not been previously reported. ‘Cheonmyeong’ sprouted and flowered 2 days earlier than the control cultivar ‘Chunpoong’, and its fruit-ripening period occurred 3 days earlier. ‘Cheonmyeong’ had 1.5-fold more stems per plant than ‘Chunpoong’. ‘Cheonmyeong’ plants were characterized by a lower occurrence of rusty root disease, and had approximately 28% higher 4-year-old root yields (571.8 ± 99.7 kg∙10a-1) than those of the rusty root-tolerant ‘Chunpoong’ (444.1 ± 110.4 kg∙10a-1). The total ginsenoside content of ‘Cheonmyeong’ (31.70 ± 1.83 mg∙g-1) was higher than that of ‘Chunpoong’ (26.62 ± 1.60 mg∙g-1). Concentrations of the ginsenosides Rg1 (which improves human memory and learning), Rf (which is effective for neuronal oscillation), and Rc (which has anti-inflammatory and analgesic effects) were higher in ‘Cheonmyeong’ than in ‘Chunpoong’. ‘Cheonmyeong’ is likely to have strong tolerance against physiological disorders, ensuring reliable production of ginseng, even in response to changes in the cultivation environment. - COLLAPSE
    February 2021