Effect of Light Intensity on Leaf Morphology,  Photosynthetic Capacity,  and Chlorophyll Content inSage (Salvia officinalis L.)

Sedigheh Rezai, Nematollah Etemadi, Ali Nikbakht, Mostafa Yousefi, and Mohamad Mahdi Majidi

doi:10.12972/kjhst.20180006

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2018 Vol.36, Issue 1 Preview Page Next Page

Effect of Light Intensity on Leaf Morphology, Photosynthetic Capacity, and Chlorophyll Content inSage (Salvia officinalis L.)

28 February 2018. pp. 46-57

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Abstract

Sage (Salvia officinalis) has been an important medicinal plant since antiquity. Here, we aimed to determine the optimum light intensity for S. officinalis growth by quantifying the effects of different shade treatments on plant morphology, chlorophyll and carotenoid content, and photosynthetic capacity in a semi-arid region of Iran. Plants were grown for 5 months under full sunlight (0% shade) as well as 30%, 50%, or 70% shade levels by applying different shade treatments. While leaf size was greatest under 50% shade level, leaves of seedlings grown under full sunlight were smallest. Leaf color under both 50% and 70% shade levels was yellowish-green. The highest photosynthesis activity was achieved under full sunlight. Decreasing light intensity led to an increase in chlorophyll and carotenoid content. Shade levels above 30% limited carbon assimilation and led to decreased essential oil content and plant growth. In conclusion, 30% shade is suggested to be an optimum light irradiance for the cultivation of S. officinalis.

Keywords

essential oil

leaf color

photoinhibition

shade

solar radiation

References

Ahlgrimm ED (1956) Beitrage zur frage der biogenese sekumdarer stoffwechselprodukte dargestellt Mentha piperita L. and Fago pyrumarten. Planta 47:255-298. doi:10.1007/BF0191515410.1007/BF01915154

Aro EM, T Hundal T, Carlberg I, Anderson B (1990) In vitro studies on light-induced inhibition of photosystem II and D1-protein degradation at low temperatures. Biochim Biophys Acta 1019:269-275. doi:10.1016/0005-2728(90)90204-H10.1016/0005-2728(90)90204-H

Bertaminia M, Muthuchelianb K, Rubinigga M, Zorera R, Velascoa R, Nedunchezhiana N (2006) Low-night temperature increased the photoinhibition of photosynthesis in grapevine (Vitis vinifera L. cv. Riesling) leaves. Environ Exp Bot 57:25-31. doi:10.1016/j.envexpbot.2005.04.00210.1016/j.envexpbot.2005.04.002

Bozin B, Mimica-Dukic N, Samojlik I, Jovin E (2007) Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L.) essential oils. J Agron Food Chem 55:7879-7885. doi: 10.1021/jf071532310.1021/jf0715323

Brand MH (1997) Shade influences plant growth, leaf color, and chlorophyll content of Kalmia latifolia L. cultivars. HortScience 32:206-208

Cantoria M, Gacutan VTC (1974) Studies on the physiology of Philippine mint (Mentha cordifolia Opiz.). II. Effect of two different light intensities on the vegetative growth and oil yield. Philippine J Sci 103:13-19

Castrillo M, Vizcaíno D, Moreno E, Latorraca Z (2005) Specific leaf mass, fresh: dry weight ratio, sugar and protein contents in species of Lamiaceae from different light environments. Rev Biol Trop 53:23-28

Chang X, Alderson PG, Wright CJ (2008) Solar irradiance level alters the growth of basil (Ocimum basilicum L.) and its content of volatile oils. Environ Exp Bot 63:216-223. doi:10.1016/j.envexpbot.2007.10.01710.1016/j.envexpbot.2007.10.017

Corre WJ (1983) Growth and morphogenesis of sun and shade plants. II. The influence of light quality. Acta Bot Neerl 32:185-202. doi:10.1111/j.1438-8677.1983.tb01700.x10.1111/j.1438-8677.1983.tb01700.x

Czeczuga B (1987) Carotenoid contents in leaves grown under various light intensities. Biochem Sys Ecol 15:523-527. doi:10.1016/0305-1978(87)90098-610.1016/0305-1978(87)90098-6

Dai Y, Shen Z, Liua Y, Wang L, Hannaway D, Lu H (2009) Effects of shade treatments on the photosynthetic capacity, chlorophyll fl uorescence, and chlorophyll content of Tetrastigma hemsleyanum Diels et Gilg. Environ Exp Bot 65:177-182. doi:10.1016/j.envexpbot.2008.12.00810.1016/j.envexpbot.2008.12.008

De Carvalho Gonçalves JF, De Sousa Barreto DC, Dos Santos JUM, Fernandes AV, Barbosa Sampaio PDT, Buckeridge MS (2005) Growth, photosynthesis and stress indicators in young rosewood plants (Aniba rosaeodora Ducke) under different light intensities. Braz J Plant Physiol 17:325-334. doi: 10.1590/S1677-0420200500030000710.1590/S1677-04202005000300007

Demming-Adams B, Adams WW (1996) Xanthophyll cycle and light stress in nature: uniform response to excess direct sunlight among higher plant species. Planta 198:460-470. doi:10.1007/BF0062006410.1007/BF00620064

Devecchi M (2006) The use of Labiatae of ornamental interest in the design of parks and gardens. Acta Hortic 723:51-57. doi:10.17660/ActaHortic.2006.723.310.17660/ActaHortic.2006.723.3

Dole JM, Wilkins HF (2004) Floriculture: Principles and species, 2nd ed., Pearson Education, Inc., Upper Saddle River, NJ, USA

Dutta PK (1971) Cultivation of Mentha arvensis in India. Flavour Ind 2:233-240

Galmes J, Medrano H, Flexas J (2007) Photosynthesis and photoinhibition in response to drought in a pubescent (var. minor) and a glabrous (var. palaui) variety of Digitalis minor. Environ Exp Bot 60:105-111. doi:10.1016/j.envexpbot.2006.08.00110.1016/j.envexpbot.2006.08.001

Ghosh PK, Ajay, Bandyopadhyay KK, Manna MC, Mandal KG, Misra AK, Hati KM (2004) Comparative effectiveness of cattle manure, poultry manure, phosphocompost and fertilizer-NPK on three cropping systems in vertisols of semi-arid tropics. II. Dry matter yield, nodulation, chlorophyll content and enzyme activity. Bioresour Technol 95:85-93. doi:10.1016/j.biortech.2004.02.01210.1016/j.biortech.2004.02.012

Gregoriou K, Pontikis K, Vemmos S (2007) Effects of reduced irradiance on leaf morphology, photosynthetic capacity, and fruit yield in olive (Olea europaea L.). Photosynthetica 45:172-181. doi: 10.1007/s11099-007-0029-x10.1007/s11099-007-0029-x

Griffin JJ, Ranney TG, Pharr DM (2004) Photosynthesis, chlorophyll fluorescence, and carbohydrate content of Illicium Texa grown under varied irradiance. J Am Soc Hortic Sci 129:46-53

Halva S, Craker LE, Simon JE, Charles DJ (1992) Light levels, growth and essential oil in dill (Anethum graveolens L.). J Herbs Spices Med Plants 1:47-58. doi:10.1300/J044v01n01_0610.1300/J044v01n01_06

Hou J, Li W, Zheng Q, Wang W, Xiao B, Xing D (2010) Effect of low light intensity on growth and accumulation of secondary metabolites in roots of Glycyrrhiza uralensis Fisch. Biochem Syst Ecol 38:160-168. doi:10.1016/j.bse.2009.12.02610.1016/j.bse.2009.12.026

Hunter RS (1975) The measurement of appearance. Wiley-Interscience, New York, USA

Keller P, Luettge U (2005) Photosynthetic light-use by three bromeliads originating from shaded sites (Ananas ananassoides, Ananas comosus cv. Panare) and exposed sites (Pitcairnia pruinosa) in the medium Orinoco basin, Venezuela. Biol Plant 49:73-79. doi:10.1007/s10535-005-3079-610.1007/s10535-005-3079-6

Kumar R, Sharma S, Pathania V (2013) Effect of shading and plant density on growth, yield and oil composition of clary sage (Salvia sclarea L. ) in north western Himalaya. J Essential Oil Res 25:23-32. doi: 10.1080/10412905.2012.74246710.1080/10412905.2012.742467

Kumar R, Sharma S, Singh B (2011) Influence of transplanting time on growth, essential oil yield and composition in clary sage (Salvia sclarea L.) plants grown under mid hills of north-western Himalayas. J Essen Oil Bearing Plants 14:260-265. doi:10.1080/0972060X.2011.1064393110.1080/0972060X.2011.10643931

Kura-Hotta R, Satoh K, Kato S (1987) Chlorophyll concentration and its changes in leaves of spinach raised under different light levels. Plant Cell Physiol 87:12-19

Lambers H, Stuart Chapin F, Pons TL (1998) Plant physiological ecology. Springer Verlag, New York. doi: 10.1007/978-1-4757-2855-210.1007/978-1-4757-2855-2

Lei TT, Tabuchi R, Kitao M, Koike T (1996) The functional relationship between chlorophyll content, leaf reflectance, and light capturing efficiency of Japanese forest species under natural shade and open light regimes. Physiol Plant 96:411-418. doi:10.1111/j.1399-3054.1996.tb00452.x10.1111/j.1399-3054.1996.tb00452.x

Li Y, Craker LE, Potter T (1996) Effect of light level on essential oil of sage (Salvia officinalis) and thyme (Thymus vulgaris). Acta Hortic 426:419-426. doi:10.17660/ActaHortic.1996.426.4610.17660/ActaHortic.1996.426.46

Lichtenhaler HK (1987) Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. In R Douce, L Packer, eds., Methods Enzymology. Academic Press Inc., New York, USA, pp 350-382

Lima CF, Andrade PB, Seabra RM, Fernandes-Ferreira M, Pereira-Wilson C (2005) The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats. J Ethnopharmacol 97:383-389. doi: 10.1016/j.jep.2004.11.02910.1016/j.jep.2004.11.029

Lima, CF, Valentao PCR, Andrade PB, Seabra RM, Fernandes-Ferreira M, Pereira-Wilson C (2007) Water and methanolic extracts of Salvia officinalis protect HepG2 cells from t-BHP induced oxidative damage. Chem Biol Interact 167:107-115. doi:10.1016/j.cbi.2007.01.02010.1016/j.cbi.2007.01.020

Mao LZ, Lu HF, Wang Q, Cai MM (2007) Comparative photosynthesis characteristics of Calycanthus chinensis and Chimonanthus praecox. Photosynthetica 45:601-605. doi:10.1007/s11099-007-0103-410.1007/s11099-007-0103-4

Mapes Ch, Xu Y (2014) Photosynthesis, vegetative habit and culinary properties of sage (Salvia officinalis) in response to low-light conditions. Canad J Plant Sci 94:881-889. doi:10.4141/cjps-2014-01010.4141/cjps-2014-010

Mattana RS, Vieira MAR, Marchese JA, Ming LCh, Marques MOM (2010) Shade level effects on yield and chemical composition of the leaf essential oil of Pothomorphe umbellata (L.) Miquel. Sci Agric 67:414-418. doi:10.1590/S0103-9016201000040000610.1590/S0103-90162010000400006

Mayer B, Baggio CH, Freitas CS, Dos Santos AC, Twardowschy A, Horst H (2009) Gastroprotective constituents of Salvia officinalis L. Fitoterapia 80:421-426. doi:10.1016/j.fitote.2009.05.01510.1016/j.fitote.2009.05.015

Mendes MM, Gazarine LC, Rodrigues ML (2001) Acclimation of Myrtus communis to contrasting Mediterranean light environments-on structure and chemical composition of foliage and plant water relations. Environ Exp Bot 45:165-178. doi:10.1016/S0098-8472(01)00073-910.1016/S0098-8472(01)00073-9

Moniruzzaman M, Islam MS, Hossain MM, Hossain T, Miah MG (2009) Effects of shade and nitrogen levels on quality Bangladhonia production. Bangladesh J Agric Res 34:205-213

Mozaffarian V (1996) A dictionary of Iranian plant names. Farhang Moaser, Tehran, Iran

Naidu RA, Krishnan M, Nayudu MV, Gnanam A (1984) Studies on peanut green mosaic virus infected peanut (Arachis hypogaea L.)leaves. II. Chlorophyll-protein complexes and polypeptide composition of thylakoid membranes. Physiol Plant Pathol 25:191-198. doi:10.1016/0048-4059(84)90057-210.1016/0048-4059(84)90057-2

Osmond CB (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In NR Baker, JR Bowyer, eds., Photoinhibition of Photosynthesis, from the Molecular Mechanisms to the Field. BIOS Scientific Publisher, Oxford, UK, pp 1-24

Pegoraro RL, De Falkenberg MB, Voltolini CH, Santos M, Paulilo MTS (2010) Production of essential oils in plants of Mentha x piperita L. var. piperita (Lamiaceae) submitted to different light levels and nutrition of the substratum. Rev Bras Bot 33:631-637. doi:10.1590/S0100-8404201000040001110.1590/S0100-84042010000400011

Pizzale L, Bortolomeazzi R, Vichi S, Überegger E, Conte LS (2002) Antioxidant activity of sage (Salvia officinalis and S. fruticosa) and oregano (Origanum onites and O. indercedens) extracts related to their phenolic compound content. J Sci Food Agric 82:1645-1651. doi:10.1002/jsfa.124010.1002/jsfa.1240

Ramalho JC, Pons TL, Groeneveld HW, Azinheira HG, Nunes MA (2000) Photosynthetic acclimation to high light conditions in mature leaves of Coffea arabica L.: role of xanthophylls, quenching mechanisms and nitrogen nutrition. Aust J Plant Physiol 27:43-51. doi:10.1071/PP9901310.1071/PP99013

Saleh M (1973) Effects of light upon quantity and quality of Matricaria chamomilla L. oil. Planta Med 24:337-340. doi:10.1055/s-0028-109950710.1055/s-0028-1099507

Santos-Gomes PC, Seabra RM, Andrade PB, Fernandes-Ferreira M (2002) Phenolic antioxidant compounds produced by in vitro shoots of sage (Salvia officinalis L.). Plant Sci 162:981-987. doi: 10.1016/S0168-9452(02)00052-310.1016/S0168-9452(02)00052-3

Schiefthaler U, Russell AW, Bolhàr-Nordenkampf HR, Critchley C (1997) Photoregulation and photodamage in Schefflera arboricola leaves adapted to different light environments. Aust J Plant Physiol 26:485-494. doi:10.1071/PP9810210.1071/PP98102

Sestak Z (1996) Liminations for finding linear relationship between chlorophyll content and photosynthetic activity. Biol Plant 8:336-346.doi:10.1007/BF0293067010.1007/BF02930670

Singh V, Sood R, Ramesh K, Singh B (2008) Effects of growth regulator application on growth, flower, oil yield, and quality of clary sage (Salvia sclarea L.). J Herbs Spices Med Plants 14:29-36. doi:10.1080/1049647080234118510.1080/10496470802341185

Sorrentino G, Cerio L, Alvino A (1997) Effect of shading and air temperature on leaf photosynthesis, fluorescence and growth in lily plants. Sci Hortic 69:259-273. doi:10.1016/S0304-4238(97)00016-210.1016/S0304-4238(97)00016-2

Taiz L, Zeiger E (2006) Plant Physiology. 4th ed., Sinauer Associates Inc., Sunderland, Massachusetts, USA, p 764

Takemiya A, Inouea S, Doi M, Kinoshita T, Shimazaki K (2005) Phototropins promote plant growth in response to blue light in low light environments. Plant Cell 17:120-127. doi:10.1105/tpc.104.03004910.1105/tpc.104.030049

Topcu G (2006) Bioactive triterpenoids from Salvia species. J Nat Prod 69:482-487. doi:10.1021/np060040210.1021/np0600402

Wang H, Wang FL, Wang G, Majourhat K (2007) The responses of photosynthetic capacity, chlorophyll fluorescence and chlorophyll content of nectarine (Prunus persica var. nectarina Maxim) to greenhouse and field grown conditions. Sci Hortic 112:66-72. doi:10.1016/j.scienta.2006.12.00710.1016/j.scienta.2006.12.007

Wang Y, Guo Q, Jin M (2009) Effects of light intensity on growth and photosynthetic characteristics of Chrysanthemum morifolium. Zhongguo Zhongyao Zazhi 34:1633-1635

Wei SL, Wang WQ, Chen XH, Qin SY, Chen XT (2005) Studies on the shade-endurance capacity of Glycyrrhiza uralensis. China J Chin Mater Med 30:100-104

Wittmann C, Aschan G, Pfanz H (2001) Leaf and twig photosynthesis of young beech (Fagus sylvatica) and aspen (Populus tremula) trees grown under different light regime. Basic Appl Ecol 2:145-154. doi: 10.1078/1439-1791-0004710.1078/1439-1791-00047

Xu DQ, Shen YK (1998) Light stress: Photoinhibition of photosynthesis in plants under natural conditions. In M Pessarakli, ed, Handbook of Plant and Crop Stress, 2nd ed, Marcel Dekker, Inc., New York, USA, pp 438-497

Yang XY, Ye XF, Liu GS, Wei HQ, Wang Y (2007) Effects of light intensity on morphological and physiological characteristics of tobacco seedlings. Chin J Appl Ecol 18:2642-2645

Zervoudakis G, Salahas G, Kaspiris G, Konstantopoulou E (2012) Influence of light intensity on growth and physiological characteristics of common sage (Salvia officinalis L.), Braz Arch Biol Technol 55:89-95. doi:10.1590/S1516-8913201200010001110.1590/S1516-89132012000100011

Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 36
No :1
Pages :46-57
Received Date :2017. 01. 12
Revised Date :2017. 10. 23
Accepted Date : 2017. 08. 29
DOI :https://doi.org/10.12972/kjhst.20180006

Horticultural Science and Technology 원예과학기술지 ISSN:1226-8763(Print) 2465-8588(Online)

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