About This Journal
About This Journal
Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provide scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.
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Research Article
- Effects of an Exogenous Melatonin Treatment on the Physiological Indexes and Storage Duration of Cut Chrysanthemum Flowers
- Bing Wang, Anqi Huang, Li Liu, Yang Li, Hua Zhang, Lei Wang
- The present study investigated the role of melatonin (MT) in improving the storage duration of cut chrysanthemum flowers. The flowers were exposed …
- The present study investigated the role of melatonin (MT) in improving the storage duration of cut chrysanthemum flowers. The flowers were exposed to different concentrations of MT and stored at 20°C ± 1°C and 90% relative humidity. Cut flowers treated with 5 µM MT had longer storage durations than those kept in water. The results demonstrated that 5 µM MT significantly enhanced the water balance and decreased water loss. The 5 µM MT treatment group exhibited a significantly higher fresh weight and flower diameter than the control group. Leaves of cut flowers treated with MT showed lower malondialdehyde (MDA) contents and lipoxygenase (LOX) activity levels than the control group. Moreover, the application of MT enhanced the antioxidant capacity of leaves by upregulating the activities of the key enzymes involved in scavenging reactive oxygen species, including superoxide dismutase, peroxidase, and catalase. This suggests that MT effectively mitigated oxidative stress and cell damage in the leaves during storage, which may contribute to its ability to extend the shelf life of cut flowers. Furthermore, 5 µM MT significantly reduced the proline content of the petals and leaves of treated flowers. These findings suggest that MT improves the storage duration most likely by regulating the antioxidant system and water balance of cut chrysanthemum flowers. - COLLAPSE
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Research Article
- Effect of Organic Nutrient Sources on the Growth and Fruit Yield of Black Chokeberry Trees
- Young-Hwa Park, Hyun-Sug Choi
- There is limited information currently available on the nutritional effects of the by-products of starfish and oil cake applied to horticultural crops. …
- There is limited information currently available on the nutritional effects of the by-products of starfish and oil cake applied to horticultural crops. This study investigated the effects of oil cake and starfish fertilizers on the performance and fruit yield of organic black chokeberry (Aronia melanocarpa (Michx.) Elliot) in South Korea between 2020 (year 1) and 2021 (year 2). The treatments included the use of 0% pelletized oil cake with 100-fold diluted starfish (0OC+SF), 33% oil cake with starfish (33OC+SF), 66% oil cake with starfish (66OC+SF), and 100% oil cake (100OC). The soil electrical conductivity, organic matter, and concentrations of total nitrogen (T-N) and P2O5 were found to be not significantly different between the treatment plots in years 1 and 2. The 66OC+SF and 100OC treatments increased foliar T-N concentrations in both years. Seasonal SPAD in the leaves increased in the 66OC+SF and 100OC treated bushes in August of year 1 and in June and September of year 2. The 0OC+SF treatment lowered the canopy volume, number of canes, and the sum of cane diameters, followed by 33OC+SF, which decreased the shoot and leaf growth but received a high light intensity from the canopy in year 2. The 66OC+SF treatment increased the fruit yield in both years, followed by the 100OC treatment. The total dry weight was determined to be greatest for the 100OC treatment bushes in year 2, while the highest fruit yield efficiency was observed for the 66OC+SF bushes. The 0OC treatment reduced the fruit length and diameter, the fresh weight, and the soluble solid content but increased the fruit surface color of lightness and yellowing. The 66OC+SF treatment was determined to be the optimum application source for moderately high leaf T-N and SPAD levels, medium-sized production of bushes, and overall high fruit productivity of black chokeberry crops. - COLLAPSE
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Research Article
- Comparison of Daily Integral Supplemental LED Lighting on Photosynthesis, Growth, and Yield of Paprika
- Heewoong Goo, Kyoung Sub Park
- Paprika (Capsicum annuum L.) has recently seen increased cultivation area and yield and is known to respond well to supplemental lighting …
- Paprika (Capsicum annuum L.) has recently seen increased cultivation area and yield and is known to respond well to supplemental lighting to enhance light conditions during periods of short-day length hours. This study evaluated methods of supplemental lighting by analyzing the photosynthetic rate, growth, and yield of paprika leaves under different lighting treatments. During a six-month cultivation period, treatment groups that utilized solar radiation sensitivity under general supplemental lighting (GSL), which operated under low solar radiation, and daily integral supplemental lighting (DISL), which operated until the target amount of light was achieved, were compared to a control group without supplemental lighting. Photosynthetic rate of paprika under both supplemental lighting conditions was higher than in control, and DISL promoted a significantly higher photosynthetic rate than other treatments. Growth analysis showed the characteristic indeterminate growth of paprika where vegetative and generative growth alternated under defoliation. The maximum relative growth rate (RGR), leaf area ratio (LAR), and specific leaf area (SLA) were initially 8 mg·g-1·d-1, 200 cm2·g-1, and 310 cm2·g-1, but they decreased as growth progressed. Net assimilation rate (NAR), which was related to the main factors in plant productivity, changed according to growth stage. Leaf area index (LAI) initially increased from 1.0 to 2.5. Crop growth rate (CGR) was the maximum at 10.2 g·m-2·d-1 when LAI was 2.4. Sugar content and yield of fruits were higher in the supplemental treatment groups than the control. These findings suggest that paprika could be efficiently cultivated even in poor light conditions by providing a light level adequate to allow morphological and physiological differentiation. - COLLAPSE
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Research Article
- γ-Aminobutyric Acid Treatment Suppresses Ethylene Biosynthesis in Actinidia aruguta during Postharvest Storage
- Qi Zhu, Xu Li, Siyi Chen, John Kojo Ahiakpa, Chao Chen, Wona Ding, Haiqiang Dong
- γ-aminobutyric acid (GABA) is known to exert significant influence on plant development. Nevertheless, there is a limited study on the modulation of …
- γ-aminobutyric acid (GABA) is known to exert significant influence on plant development. Nevertheless, there is a limited study on the modulation of kiwifruit ripening through the use of GABA. In this study, we conducted an assessment of the physicochemical properties and gene expression patterns associated with ripening in hardy kiwifruit (Actinidia aruguta). Specifically, the result showed that the application of exogenous GABA had a positive impact on kiwifruit quality. Additionally, it was observed that exogenous GABA treatment led to a decrease in respiration rate and ethylene production. Furthermore, the enzyme activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase (ACO) and ACC synthase (ACS) were reduced as a result of the exogenous GABA treatment. Conversely, the overexpression of the AaGAD1 or AaGAD4 gene, which plays a role in the biosynthesis of GABA, resulted in the inhibition of ethylene production by decreasing the the expression levels of AaACO1, AaACO3, AaACS1, and AaACS2. The silencing of the AaGAD1 or AaGAD4 gene had the opposite effect. Our findings provide evidence that the application of exogenous GABA has the potential to enhance the storage quality and extend the shelf life of kiwifruit. Thus, the study confirms the involvement of GABA in molecular-level regulation of ethylene production in kiwifruit. - COLLAPSE
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Research Article
- Selection of Eucalyptus Tree Species and Soil Management Suitable for the Environment of Southern South Korea
- Jong-Hwa Gu, Byung-Jin Choi, Hyun-Sug Choi
- The study was initiated to examine suitable young Eucalyptus tree species, E. gunnii, E. parvula, and E. pulverulenta, through …
- The study was initiated to examine suitable young Eucalyptus tree species, E. gunnii, E. parvula, and E. pulverulenta, through the plantation adaptation potential and to compare a straw mulch (SM) treatment with a non-mulch (NM) treatment for early tree establishment in southern South Korea by 2022 and 2023. Rapid climate swings with freezing conditions occurred frequently in January and February of 2023, with heavy rainfall and drought in spring and summer, respectively. The SM treatment resulted in soil moisture conservation and softer soil than the NM treatment. The height of E. parvula-SM trees increased significantly to 175.3 cm from 180 to 480 days after planting (DAP), followed by the E. parvula-NM (148.5 cm), E. gunnii-SM (132.0 cm), and E. pulverulenta-SM (102.0 cm) cases, with severe dieback occurring in E. pulverulenta-NM. In addition, similar seasonal growth patterns were observed on the tree trunk and in the number of shoots and leaves. Foliar SPAD declined slightly from 240 to 300 DAP during the cold winters and gradually increased or remained constant thereafter. The highest readings were observed for E. gunnii and the lowest were found for E. pulverulenta. Total plant biomass was highest in E. parvula-SM tree (290.8 g) and lowest in parvula-NM (0.0 g) and E. gunnii-NM trees (29.3 g). Leaf browning occurred in more than 80% of E. pulverulenta and E. gunnii trees, in 58.6% of E. parvula-NM trees, and in 58.2% of E. parvula-SM trees. Seasonal tree survival worsened from 0 to 480 DAP and was highest at 480 DAT in the following order: E. parvula-SM (75%) and -NM (50%), E. gunnii-SM (27%), E. pulverulenta-SM (25%), E. gunnii-NM (23%), and E. pulverulenta-NM (0%). Populations of beet armyworms developed with all Eucalyptus species from 50 to 70 DAP, with a 10–20% higher incidence occurring in E. pulverulenta-NM than in E. gunnii or E. parvula-SM and -NM. Leaves on the stem cuttings of E. gunnii-SM retained their weight and SPAD values 15 days after harvest, minimizing postharvest deterioration of the cut foliage. Overall, E. parvula is a suitable species when cultivated with straw strips for soil management, as this species shows enhanced tree production and recovery after exposure to chilling stress during the first two years of establishment. - COLLAPSE
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Research Article
- Screening of Pepper and Tomato Grafted Seedling Cultivars for Intumescence Susceptibility
- Ji Hye Yun, So Yeong Hwang, Jin Yu, Jeong Hun Hwang, Eun Won Park, Hyeon Woo Jeong, Hee Sung Hwang, Seung Jae Hwang
- Intumescence is a physiological disorder in which epidermal cells become enlarged, thereby decreasing the commercial value of crops. This disorder occurs under …
- Intumescence is a physiological disorder in which epidermal cells become enlarged, thereby decreasing the commercial value of crops. This disorder occurs under low-UV and high-humidity conditions, similar to the environment in the LED healing chambers used for grafted plug seedling production. In addition, the incidence of intumescence can differ depending on the cultivar involved. Therefore, in this study, different pepper (Capsicum annuum L.) and tomato (Solanum lycopersicum L.) cultivars were grafted to identify those susceptible to intumescence. The pepper and tomato seedlings in this study were grafted the same day and were immediately moved into an LED healing chamber. From the results, intumescence was observed in all pepper cultivars. The pepper cultivar ‘Sunhan Gilsang’ was found to be highly susceptible to intumescence given its highest incidence of intumescence in leaf. However, tomatoes only occurred in ‘TY Haruakki’ and ‘TTM-130’. The results of this study can serve as primary data for those in charge of selecting experimental cultivars to prevent intumescence in pepper and tomato-grafted seedlings. - COLLAPSE
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Research Article
- Analysis of Evapotranspiration using a Load Cell and Precise Irrigation Amounts for Strawberries in a Coir Slab Hydroponic System
- Kyeong Ho Kim, Minkyung Kim, MG Rabbani, Yong-Beom Lee, Ki Young Choi
- Strawberries in Korea hold significant economic value, and their cultivation in hydroponics is progressively expanding. This study explored the weighted evapotranspiration (WETc) …
- Strawberries in Korea hold significant economic value, and their cultivation in hydroponics is progressively expanding. This study explored the weighted evapotranspiration (WETc) of strawberries grown on coir substrates, with the goal of optimizing irrigation volumes for improved water management. ‘Sulhyang’ strawberries were transplanted at a plant density of 7.5 plants per m2 on September 15, 2022 into three different substrate types: V40D10 (40 L, dust: chip (v/v) = 10:0), V30D10 (30 L, dust: chip (v/v) = 10:0), and V20D5 (20 L, dust: chip (v/v) = 5:5). The nutrient solution was administered through an ISR-automated irrigation system. The schedule concentrated irrigation from sunrise to 2 p.m. (accounting for 78–95% of the total daily irrigation volume), resulting in higher drainage rates from 11 a.m. to 2 p.m. (50–70% of the day’s total drained volume). The drainage rate for V20D5 was higher, at 40–50%. Total fruit weight per plant varied significantly across the substrates, with V30D10 producing the highest weight (428.4 g) and V40D10 the lowest (315.5 g). Despite lower dust ratios, V20D5 achieved performance comparable to that of V30D10, with a total fruit weight of 350 g. An analysis of WETc on sunny days showed a pattern of February > January > December, with hourly WETc revealing significant differences among the substrates. On cloudy days, WETc remained below 5 g·plant-1, with cumulative values ranging from 41 to 75 g·plant-1. Overall, the results suggest that the optimal daily irrigation volumes per plant should be between 90 to 150 mL·plant-1 depending on the substrate volume and ratio. These findings underscore the potential to minimize drainage and optimize irrigation in strawberry hydroponics through precise, WETc-based scheduling, providing valuable insights for sustainable cultivation practices. - COLLAPSE
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Research Article
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Ammonia Emissions, Soil Chemical Properties and Fruit Quality According to the Soil Supply of Urea and Organic Fertilizer in a ‘Campbell Early’ Vineyard
‘캠벨얼리’ 포도원에서 요소 및 유기질비료 토양 공급에 의한 암모니아 배출량과 토양 화학성 및 과실품질
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Young-Ji Moon, Byung-Woo Moon, Hyun-Hwoi Ku
문영지, 문병우, 구현회
- This study was carried out in order to investigate ammonia emissions, soil chemical properties and fruit quality according to urea fertilizer (UR) …
포도 비가림 시설에서 ‘Campbell Early’ 재배 시 요소비료(UR) 및 유기질비료(OF) 토양 공급에 의한 암모니아 배출량 산정, 토양 화학성 및 과실품질에 미치는 영향은 …
- This study was carried out in order to investigate ammonia emissions, soil chemical properties and fruit quality according to urea fertilizer (UR) and organic fertilizer (OF) applications under a rain-proof cultivation system in an ‘Campbell Early’ vineyard. The results showed that ammonia emissions in the UR2 (200 kg N·ha-1) plot began to increase rapidly from the first day of the treatment, peaked at the maximum level (1.38 kg N·ha-1·day-1) on the seventh day, and then gradually decreased, after which the emissions increased again on the twenty-first day and then has gradually decreased. The plot treated with OF4 (454 kg N·ha-1) reached a maximum emission level of 1.22 kg N·ha-1 day-1 on the first day of application, and then gradually decreased. The cumulative ammonia emissions (NH3·ha-1 per season) for 155 days after soil application of the UR2 and OF4 fertilizers were 141.3 kg for the UR2 plot, and 161.1 kg for the OF4 plot. There were no differences in soil chemical properties such as pH, EC, OM, or T-N between the treatments. However, the P concentration in the leaf stalk of the OF4 plot was higher than those of the other plots. The K concentration of the fruit skin was also higher in the OF3 plot than in the untreated plot. Yields were increased by 15.1%, 17.9, and 18.5% in the UR2, OF3, and OF4 cases, respectively, whereas there were no differences in the fruit quality at harvest. In this study, an organic fertilizer was found to increase fruit yields, although it increased ammonia emissions as well. Therefore, we recommend that an appropriate source and appropriate amount of N should be carefully applied to increase fruit yields while reducing ammonia emissions during cultivation in ‘Campbell Early’ vineyards.
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포도 비가림 시설에서 ‘Campbell Early’ 재배 시 요소비료(UR) 및 유기질비료(OF) 토양 공급에 의한 암모니아 배출량 산정, 토양 화학성 및 과실품질에 미치는 영향은 다음과 같다. UR2(200kg N·ha-1) 공급에 의한 암모니아 배출량은 1일째부터 급격히 증가하기 시작하여 7일째 최대 배출량(1.35kg N·ha-1·day-1)을 이루다가 점차 감소하다가 21일째에 다시 증가한 후 점차 감소하였다. OF4(454kg N·ha-1) 토양 공급은 처리 후 1일째에 최대 배출량 1.22kg N·ha-1 day-1에 도달하였으며 그 이후는 점차 감소하였다. UR2와 OF4 비료를 토양 공급 후 155일 동안 누적 암모니아 발생량은 UR2 처리에서 141.3kg과 OF4 처리에서 161.1kg NH3·ha-1 per season을 나타내었다. 처리별 토양 화학성은 차이는 없었으나, 엽병에서 P 농도는 다른 처리에 비하여 OF4 처리가 높았다. 또한 과피의 K 농도는 무처리에 비하여 OF3 처리에서 높았다. 10a 수량은 UR2 토양 공급에 의해 15.1%, OF 처리는 17.9–18.5% 증가하였으나, 수확 시 과실품질은 처리간 차이는 없었다. 이와 같은 결과는 ‘Campbell Early’ 과수원에서 암모니아 배출량 저감을 위한 무기질 및 유기질비료 시용의 기초자료로 활용할 수 있을 것이다.
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Ammonia Emissions, Soil Chemical Properties and Fruit Quality According to the Soil Supply of Urea and Organic Fertilizer in a ‘Campbell Early’ Vineyard
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Research Article
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Effect of the Degassed Status on the Quality and Microbial Control of Enoki Mushrooms (Flammulina velutipes) for Export to the U.S.
미국 수출을 위한 팽이버섯의 탈기 정도가 품질 및 미생물 제어에 미치는 영향
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Han Ryul Choi, Ji Weon Choi, Yeo Eun Yun, Ji Hyun Lee, Sooyeon Lim
최한률, 최지원, 윤여은, 이지현, 임수연
- The decline in the export of Korean enoki mushrooms (Flammulina velutipes) to the United States has been linked to rising …
한국 팽이버섯의 미국 수출이 감소한 원인은 소비자들의 불만과 미생물 안전에 대한 우려와 연관되어 있다. 이는 주로 유통 과정에서 품질의 저하로 인해 발생되며, …
- The decline in the export of Korean enoki mushrooms (Flammulina velutipes) to the United States has been linked to rising dissatisfaction among consumers and concerns over microbial safety. This is primarily due to quality deterioration during the distribution process, leading to inadequate conditions for maintaining the freshness and safety standards of the mushrooms. In this study, we investigated the effects of pressed packaging (PP) and semi-pressed packaging (SPP) on the quality, sensory evaluation, and microbial control of enoki mushrooms for export to the United States during a simulated shipping period of three weeks at 4°C and a local distribution period of one week at 10°C. Common quality degradation factors such as the browning index (BI), respiration rate, off odors, browning, cap opening, stem elongation, total aerobic count, and environmental Listeria spp. count were identified for both PP and SPP during simulated shipping. There were also significant differences between PP and SPP regarding SSC, pH, and BI. The sensory evaluation showed that browning, cap opening, and stem elongation were significantly higher in SPP than in PP, making it difficult for semi-vacuum packaging to be commercialized for three weeks at 4°C and one week at 10°C. Regarding microbial safety, PP significantly suppressed the growth of harmful microorganisms such as the total aerobic count and environmental Listeria spp. count, providing an advantage with regard to preserving the quality. In conclusion, PP was more effective than SPP in suppressing the occurrence of harmful microorganisms and improving the respiration rate, BI, browning, cap opening, stem elongation, total aerobic count, and environmental Listeria spp. count outcomes, thus helping to maintain the safety and quality of enoki mushrooms during their distribution.
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한국 팽이버섯의 미국 수출이 감소한 원인은 소비자들의 불만과 미생물 안전에 대한 우려와 연관되어 있다. 이는 주로 유통 과정에서 품질의 저하로 인해 발생되며, 이로 인해 팽이버섯의 신선도와 안전 기준을 유지하기에 적합하지 않은 상태로 이어진다. 본 연구는 팽이버섯을 미국으로 수출하기 위한 진공 포장(PP)과 반 진공 포장(SPP)의 품질, 관능 평가 및 미생물 안전성에 대한 영향을 조사하였다. 이를 위해 모의 수송 기간인 3주 수송 기간(4°C)과 1주 현지 유통 기간(10°C)을 적용하여 실험을 진행하였다. BI, 호흡률, 이취, 갈변, 갓 개열, 줄기 신장, 일반 세균 및 환경 리스테리아는 모의 선박 수송 중 진공 포장(PP) 및 반 진공 포장(SPP) 모두에서 공통적인 품질 저하 인자로 선정되었다. 진공 포장(PP) 및 반 진공 포장(SPP) 사이에서 SSC, pH 및 BI에서 유의한 차이가 확인되었다. 관능 평가에서는 갈변, 갓 개열, 줄기 신장이 진공 포장(PP)에 비해 반 진공 포장(SPP)에서 유의하게 높았으며, 이로 인해 3주 수송 기간(4°C) 과 1주 현지 유통 기간(10°C)에서 반 진공 포장(SPP)이 상품화가 어려움을 확인하였다. 미생물 안전성 측면에서, 진공포장(PP)은 일반 세균 및 환경 리스테리아와 같은 유해 미생물의 성장을 유의하게 억제하여 품질 보존에 이점을 제공하였다. 결론적으로, 진공포장(PP)는 반 진공 포장(SPP)에 비해 호흡률, BI, 갈변, 갓 개열, 줄기 신장, 일반 세균 및 환경 리스테리아와 같은 유해 미생물의 발생을 억제하여 팽이버섯의 안전성과 품질 유지에 도움이 되었다.
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Effect of the Degassed Status on the Quality and Microbial Control of Enoki Mushrooms (Flammulina velutipes) for Export to the U.S.
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Research Article
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Selection of Differentially Expressed Genes Responding to High Temperatures in ‘Fuji’ Apple Tree Leaves by a Transcriptome Analysis
‘후지’ 사과나무 잎의 전사체 분석을 통한 고온에 특이적으로 반응하는 유전자 선발
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Soon Young Ahn, Zar Le Myint, Seon Ae Kim, Seung Heui Kim, Hae Keun Yun
안순영, 자리민, 김선애, 김승희, 윤해근
- A transcriptome analysis was conducted to select differentially expressed genes (DEGs) from ‘Fuji’ apple tree leaves exposed to high temperatures (35°C) for …
전사체 분석을 이용하여 고온처리 한 사과나무 후지 잎에 차등발현하는 유전자를 확인하였다. 품질이 낮은 시퀀스를 제거한 후 25°C와 35°C 처리구에서 각각 총 34,158,132와 …
- A transcriptome analysis was conducted to select differentially expressed genes (DEGs) from ‘Fuji’ apple tree leaves exposed to high temperatures (35°C) for 24 hours. A total of 34,158,132 and 41,475,948 paired-end reads for 25°C and 35°C samples of ‘Fuji’ apple tree leaves were obtained, respectively. The average read-length ranged from 90.0 to 90.2 bp and the analyzed reads were found to cover 82.8-83.1% of the raw data. DEGs were divided into 15, 11, and 30 main functional categories of molecular functions, cellular components, and biological processes with different degrees at level 3, respectively. The major up-regulated genes included a GDSL-like lipase/acylhydrolase superfamily protein, a cell wall protein precursor, MLP-like protein 423, heat shock protein 18.2, a HSP20-like chaperones superfamily protein, phytosulfokine receptor 1, a protein phosphatase 2C family protein, and microsomal glutathione s-transferase, while the major down-regulated genes included 3-ketoacyl-CoA synthase 19, a cysteine proteinase superfamily protein, the plant invertase/ pectin methylesterase inhibitor heat shock protein 70, ferretin 1, nitrate transporter 1.7, and heat shock protein 81-2 in apple leaves exposed to high temperatures. The selected DEGs were confirmed to be differentially expressed by high temperatures in a quantitative real time-PCR analysis with gene-specific primers. Transcriptome data can provide meaningful information about the discovery of specific gene and can elucidate the mechanism in apple trees responding to high temperatures, and selected DEGs can be used as genetic resources in the molecular breeding of apples tolerant to foreign stress sources such as high temperatures in the future.
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전사체 분석을 이용하여 고온처리 한 사과나무 후지 잎에 차등발현하는 유전자를 확인하였다. 품질이 낮은 시퀀스를 제거한 후 25°C와 35°C 처리구에서 각각 총 34,158,132와 41,475,948의 transcripts를 확보하였다. 평균 길이는 25°C와 35°C 처리구에서 각각 90.0bp와 90.2bp이며, 평균 82.9%의 data가 분석에 활용되었다. 전사체 분석을 이용하여 24시간 동안 고온에 처리된 후지 사과나무 잎에서 차등발현(Differentially expressed genes)되는 유전자를 확인하였다. DEG는 3개의 주요 category인 biological processes, cellular components, molecular functions으로 나눠졌다. 고온에 노출된 사과나무 잎에서 발현이 유도되는 주요 유전자들은 GDSL-like lipase/acylhydrolase superfamily protein, cell wall protein precursor, MLP-like protein 423, heat shock protein 18.2, HSP20-like chaperone superfamily protein, phytosulfokin receptor 1, protein phosphatase 2C family protein, and microsomal glutathione s-transferase 등 이였으며, 반면 발현이 억제되는 유전자들은 3-ketoacyl-CoA synthase 19, cysteine proteinases superfamily protein, plant invertase/pectin methylesterase inhibitor, heat shock protein 70, ferretin 1, nitrate transporter 1.7, and heat shock protein 81-2 등 이였다. 모든 DEG는 gene-specific primer를 이용하여 quantitative real time-PCR로 검증하였다. 고온 처리 사과나무 잎의 전사체 분석 결과는 향후 연구를 위한 특이 유전자 발굴이나 고온 처리에 대한 사과나무 잎의 반응 기작을 설명하는데 있어 의미 있는 정보를 제공할 것이다.
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Selection of Differentially Expressed Genes Responding to High Temperatures in ‘Fuji’ Apple Tree Leaves by a Transcriptome Analysis
Horticultural Science and Technology
