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Research Article

Assessment of Genetic and Floral Morphological Uniformity in Tissue-Culture-Derived Clones of Hibiscus syriacus ‘Nanpa’
Ji-Hi Son1
,
Hyeon-Jin Sun2
,
Hyo-Yeon Lee12*
1Department of Biotechnology, Jeju National University, Jeju 63243, Korea
2Subtropical Horticulture Research Institute, Jeju National University, Jeju 63243, Korea
*Corresponding Author
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Abstract

In this study, we evaluated the genetic and floral morphological uniformity of clonal Hibiscus syriacus ‘Nanpa’ plants regenerated through tissue culturing using mature green leaves. Morphological traits and random amplified polymorphic DNA banding patterns were analyzed to assess variations among clones. Most clones showed highly uniform floral morphologies and genetic profiles. However, somaclonal variations were observed in Clones 25, 28, and 36, which exhibited deviations in both the molecular markers and morphological characteristics. These results highlight the dual potential of tissue culturing when used to generate novel genetic variants and the importance of verifying genetic fidelity during micropropagation. The combined use of morphological evaluations and molecular marker analysis ensures clonal stability in commercial propagation systems. This study provides insights into somaclonal variations in H. syriacus and supports the application of tissue culture techniques in plant breeding and large-scale production.

Keywords
cluster analysis
genetic fidelity in micropropagation
genetic stability
molecular marker analysis
somaclonal variation
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Received Date : 2025-11-03
  • Revised Date : 2026-01-19
  • Accepted Date : 2026-01-26
  • DOI :https://doi.org/10.7235/HORT.20260004
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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