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2025 Vol.43, Issue 4 Preview Page

Research Article

Research on the Establishment of Strawberry Stem Tip Tissue Cultivation and In-vitro Rapid Micropropagation System
Guoye Guo1*
,
Wenxue Li2
,
Yichen Liu1
,
Xiaoyi Jing1
,
Manyu Su1
,
Yuxuan Ding1
,
Jiaying Wu1
1College of Water Resources and Modern Agriculture, Nanyang Normal University, Nanyang 473061, China
2College of Life Science, Zhengzhou University, Zhengzhou 450001, China
*Corresponding Author
31 August 2025. pp. 411-420
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Abstract

The creeping shoot tips of strawberry varieties “Shengdanhong” and “Hongyan” were cultivated in vitro to develop protocols for expeditious and reliable micropropagation. The morphogenetic response of varying concentrations and combinations of different hormones on shoot tip germination, proliferation and rooting were studied. The results showed that the most suitable shoot tip induction medium for “Shengdanhong” was MS media containing 0.5 mg·L-1 6-BA and 0.2 mg·L-1 IBA, while for “Hongyan” it was MS media containing 1.0 mg·L-1 6-BA and 0.1 mg·L-1 IBA, with corresponding germination rates of 84.21% and 88.10%. The optimal subculture adventitious bud proliferation outcome had been cultured in MS media supplemented with 0.2 mg·L-1 6-BA and 0.1 mg·L-1 NAA, with proliferation coefficients were 6.11 and 7.13, respectively. The highest corresponding rooting rates were 72.41% and 91.67% for “Shengdanhong” and “Hongyan”, as cultured in 1/2MS medium containing 0.5 mg·L-1 IBA and 2 mg·L-1 AC, and 1/2MS containing 0.5 mg·L-1 IBA, respectively. Virus detection of regenerated seedlings by RT-PCR showed that the SMoV and SCV detoxification rates were 92.5% and 100%, respectively. These examined findings contribute valuable insights into the mass production of virus-free strawberry plantlets using in vitro culture techniques.

키워드
multiplication culture
rooting culture
RT-PCR
shoot tip
strawberry
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :4
  • Pages :411-420
  • Received Date : 2024-10-02
  • Revised Date : 2025-01-24
  • Accepted Date : 2025-02-12
  • DOI :https://doi.org/10.7235/HORT.20250037
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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