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2022 Vol.40, Issue 1 Preview Page

Research Article

Differential Responses of Capsicum Accessions to Foliar Blight and Root Rot by Phytophthora capsici
Won-Hee Kang1†
,
Jae-Young Nam1†
,
Ji-Su Kwon2
,
Seon-In Yeom12*
1Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Korea
2Department of Horticulture, Division of Applied Life Science (BK21 Four Program), Gyeongsang National University, Jinju 52828, Korea
*Corresponding Author
†These authors contributed equally to this work.
28 February 2022. pp. 85-93
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Abstract

The oomycete Phytophthora capsici is one of the most destructive pepper pathogens, reducing product quality and resulting in major yield losses in Capsicum spp. To date, little is known about the defense responses of the host pepper against different P. capsici isolates. Although foliar blight and root rot are caused by the same pathogen, the host resistance responses may differ among disease symptoms and must be studied independently. In this study, 30 Capsicum accessions were inoculated with two P. capsici isolates by root drenching, and the severity of root rot was evaluated. In addition, one P. capsici strain was injected into the leaves of the same 30 accessions to evaluate the difference in host defense responses to foliar blight and root rot caused by the same strain. Among 30 accessions, 6 showed different resistance levels to the two P. capsici isolates. When comparing the resistance levels to root rot and foliar blight, 14 pepper accessions showed different resistance responses based on the inoculation site. These results suggest that the resistance to foliar blight in peppers differs according to the isolate of P. capsici and the inoculation site, even within the same pepper cultivar. The results of this study will provide useful information for the breeding of Phytophthora root rot and foliar blight-resistant Capsicum peppers.

Keywords
defense response
leaf infiltration
Phytophthora blight
resistant screening
root drench
References
1
Barchenger DW, Lamour KH, Bosland PW (2018) Challenges and strategies for breeding resistance in Capsicum annuum to the multifarious pathogen, Phytophthora capsici. Front Plant Sci 9:628. doi:10.3389/fpls.2018.00628 10.3389/fpls.2018.0062829868083PMC5962783
2
Bouwmeester K, Govers F (2009) A novel method for efficient and abundant production of Phytophthora brassicae zoospores on Brussels sprout leaf discs. BMC Plant Biol 9:111. doi:10.1186/1471-2229-9-111 10.1186/1471-2229-9-11119698127PMC2752460
3
Candole BL, Conner PJ, Ji PS (2010) Screening Capsicum annuum accessions for resistance to six isolates of Phytophthora capsici. Hortscience 45:254-259. doi:10.21273/HORTSCI.45.2.254 10.21273/HORTSCI.45.2.254
4
Chunthawodtiporn J, Hill T, Stoffel K, Van Deynze A (2018) Quantitative trait loci controlling fruit size and other horticultural traits in bell pepper (Capsicum annuum). Plant Genome 11:160125. doi:10.3835/plantgenome2016.12.0125 10.3835/plantgenome2016.12.012529505638
5
Food and Agriculture Organization of the United Nations (FAOSTAT) (2018) FAOSTAT. http://www.fao.org/faostat/en/#data/QC. Accessed 14 September 2020
6
Foster J, Hausbeck M (2010) Resistance of pepper to Phytophthora crown, root, and fruit rot is affected by isolate virulence. Plant Disease 94:24-30. doi:10.1094/PDIS-94-1-0024 10.1094/PDIS-94-1-002430754398
7
Glosier BR, Ogundiwin EA, Sidhu GS, Sischo DR, Prince JP (2007) A differential series of pepper (Capsicum annuum) lines delineates fourteen physiological races of Phytophthora capsici. Euphytica 162:23-30. doi:10.1007/s10681-007-9532-1 10.1007/s10681-007-9532-1
8
Granke LL, Quesada-Ocampo L, Lamour K, Hausbeck MK (2012) Advances in research on Phytophthora capsici on vegetable crops in the United States. Plant Dis 96:1588-1600. doi:10.1094/PDIS-02-12-0211-FE 10.1094/PDIS-02-12-0211-FE30727465
9
Hausbeck MK, Lamour KH (2004) Phytophthora capsici on vegetable crops: research progress and management challenges. Plant Dis 88:1292-1303. doi:10.1094/PDIS.2004.88.12.1292 10.1094/PDIS.2004.88.12.129230795189
10
Howard LR, Wildman RE (2007) Antioxidant vitamin and phytochemical content of fresh and processed pepper fruit (Capsicum annuum). In RC Wildman, ed, Handbook of Nutraceuticals and Functional Foods, Ed 2, CRC Press, Baca Raton, FL, USA, pp 209-233. doi:10.1201/9781420006186.ch9 10.1201/9781420006186.ch9
11
Jo SJ, Shim SA, Jang KS, Choi YH, Kim J-C, Choi GJ (2014) Resistance of chili pepper cultivars to isolates of Phytophthora capsici. Korean J Hortic Sci Technol 32:66-76. doi:10.7235/hort.2014.13079 10.7235/hort.2014.13079
12
Kang WH, Lee J, Koo N, Kwon JS, Park B, Kim YM, Yeom SI (2022) Universal gene co-expression network reveals receptor-like protein genes involved in broad-spectrum resistance in pepper (Capsicum annuum L.). Hortic Res uhab003. doi:10.1093/hr/uhab003 10.1093/hr/uhab00335043174
13
Kang WH, Sim YM, Koo N, Nam JY, Lee J, Kim N, Jang H, Kim YM, Yeom SI (2020) Transcriptome profiling of abiotic responses to heat, cold, salt, and osmotic stress of Capsicum annuum L. Sci Data 7:17. doi:10.1038/s41597-020-0352-7 10.1038/s41597-020-0352-731932587PMC6957515
14
Kang WH, Yeom SI (2018) Genome-wide identification, classification, and expression analysis of the receptor-like protein family in tomato. Plant Pathol J 34:435-444. doi:10.5423/PPJ.OA.02.2018.0032 10.5423/PPJ.OA.02.2018.003230369853PMC6200040
15
Kim DH, Kang WH, Yeom SI, Kim BD (2019a) Isolation of putative pepper defense-related genes against the pathogen Phytophthora capsici using suppression subtractive hybridization/macroarray and RNA-sequencing analyses. Hortic Environ Biotechnol 60:685-699. doi:10.1007/s13580-019-00157-1 10.1007/s13580-019-00157-1
16
Kim N, Kang WH, Lee J, Yeom SI (2019b) Development of clustered resistance gene analogs-based markers of resistance to Phytophthora capsici in chili pepper. Biomed Res Int 2019:1093186. doi:10.1155/2019/1093186 10.1155/2019/109318630719438PMC6335758
17
Kim S, Park J, Yeom SI, Kim YM, Seo E, Kim KT, Kim MS, Lee JM, Cheong K, et al (2017b) New reference genome sequences of hot pepper reveal the massive evolution of plant disease-resistance genes by retroduplication. Genome Biol 18:210. doi:10.1186/s13059-017-1341-9 10.1186/s13059-017-1341-929089032PMC5664825
18
Kim SB, Kang WH, Huy HN, Yeom SI, An JT, Kim S, Kang MY, Kim HJ, Jo YD, et al (2017a) Divergent evolution of multiple virus-resistance genes from a progenitor in Capsicum spp. New Phytol 213:886-899. doi:10.1111/nph.14177 10.1111/nph.1417727612097
19
Kwon JS, Nam JY, Yeom SI, Kang WH (2021) Leaf-to-whole plant spread bioassay for pepper and Ralstonia solanacearum interaction determines inheritance of resistance to bacterial wilt for further breeding. Int J Mol Sci 22:2279. doi:10.3390/ijms22052279 10.3390/ijms2205227933668965PMC7956186
20
Lamour KH, Stam R, Jupe J, Huitema E (2012) The oomycete broad-host-range pathogen Phytophthora capsici. Mol Plant Pathol 13:329-337. doi:10.1111/j.1364-3703.2011.00754.x 10.1111/j.1364-3703.2011.00754.x22013895PMC6638677
21
Lee HA, Yeom SI (2015) Plant NB-LRR proteins: tightly regulated sensors in a complex manner. Brief Funct Genomics 14:233-242. doi:10.1093/bfgp/elv012 10.1093/bfgp/elv01225825425
22
Lee J, Nam JY, Jang H, Kim N, Kim YM, Kang WH, Yeom SI (2020) Comprehensive transcriptome resource for response to phytohormone-induced signaling in Capsicum annuum L. BMC Res Notes 13:440. doi:10.1186/s13104-020-05281-1 10.1186/s13104-020-05281-132943083PMC7499990
23
Liu WY, Kang JH, Jeong HS, Choi HJ, Yang HB, Kim KT, Choi D, Choi GJ, Jahn M, et al (2014) Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper. Theor Appl Genet 127:2503-2513. doi:10.1007/s00122-014-2394-8 10.1007/s00122-014-2394-825208646
24
Loizzo MR, Pugliese A, Bonesi M, Menichini F, Tundis R (2015) Evaluation of chemical profile and antioxidant activity of twenty cultivars from Capsicum annuum, Capsicum baccatum, Capsicum chacoense and Capsicum chinense: A comparison between fresh and processed peppers. LWT-Food Sci Technol 64:623-631. doi:10.1016/j.lwt.2015.06.042 10.1016/j.lwt.2015.06.042
25
Monroy-Barbosa A, Bosland PW (2010) A rapid technique for multiple-race disease screening of Phytophthora foliar blight on single Capsicum annuum L. Plants. HortScience 45:1563-1566. doi:10.21273/HORTSCI.45.10.1563 10.21273/HORTSCI.45.10.1563
26
Oelke LM, Bosland PW, Steiner R (2003) Differentiation of race specific resistance to Phytophthora root rot and foliar blight in Capsicum annuum. J Am Soc Hortic Sci 128:213-218. doi:10.21273/JASHS.128.2.0213 10.21273/JASHS.128.2.0213
27
Ogundiwin EA, Berke TF, Massoudi M, Black LL, Huestis G, Choi D, Lee S, Prince JP (2005) Construction of 2 intraspecific linkage maps and identification of resistance QTLs for Phytophthora capsici root-rot and foliar-blight diseases of pepper (Capsicum annuum L.). Genome 48:698-711. doi:10.1139/g05-028 10.1139/g05-02816094437
28
Oh SK, Baek KH, Seong ES, Joung YH, Choi GJ, Park JM, Cho HS, Kim EA, Lee S, et al (2010) CaMsrB2, pepper methionine sulfoxide reductase B2, is a novel defense regulator against oxidative stress and pathogen attack. Plant Physiol 154:245-261. doi:10.1104/pp.110.162339 10.1104/pp.110.16233920643759PMC2938166
29
Rehrig WZ, Ashrafi H, Hill T, Prince J, Van Deynze A (2014) CaDMR1 Cosegregates with QTL Pc5.1 for resistance to Phytophthora capsici in pepper (Capsicum annuum). Plant Genome 7. doi:10.3835/plantgenome2014.03.0011 10.3835/plantgenome2014.03.0011
30
Reifschneider F, Boiteux L, Della Vecchia P, Poulos J, Kuroda N (1992) Inheritance of adult-plant resistance to Phytophthora capsici in pepper. Euphytica 62:45-49. doi:10.1007/BF00036086 10.1007/BF00036086
31
Silvar C, Merino F, Diaz J (2006) Diversity of Phytophthora capsici in northwest Spain: analysis of virulence, metalaxyl response, and molecular characterization. Plant Dis 90:1135-1142. doi:10.1094/PD-90-1135 10.1094/PD-90-113530781092
32
Thabuis A, Palloix A, Servin B, Daubeze A, Signoret P, Lefebvre V (2004) Marker-assisted introgression of 4 Phytophthora capsici resistance QTL alleles into a bell pepper line: validation of additive and epistatic effects. Mol Breed 14:9-20. doi:10.1023/B:MOLB.0000037991.38278.82 10.1023/B:MOLB.0000037991.38278.82
33
Yeom SI, Baek HK, Oh SK, Kang WH, Lee SJ, Lee JM, Seo E, Rose JK, Kim B-D, et al (2011) Use of a secretion trap screen in pepper following Phytophthora capsici infection reveals novel functions of secreted plant proteins in modulating cell death. Mol Plant Microbe Interact 24:671-684. doi:10.1094/MPMI-08-10-0183 10.1094/MPMI-08-10-018321542767
34
Yeom SI, Seo E, Oh SK, Kim KW, Choi D (2012) A common plant cell-wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens. Plant J 69:755-768. doi:10.1111/j.1365-313X.2011.04828.x 10.1111/j.1365-313X.2011.04828.x22023393
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 40
  • No :1
  • Pages :85-93
  • Received Date : 2020-12-03
  • Revised Date : 2021-03-11
  • Accepted Date : 2021-04-21
  • DOI :https://doi.org/10.7235/HORT.20220009
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