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2022 Vol.40, Issue 1 Preview Page

Research Article

Development of an Efficient Screening Method for Chinese Cabbage Cultivars Resistant to Alternaria brassicicola and Determination of Their Resistance Characteristics

Alternaria brassicicola에 대한 배추의 효율적인 저항성 검정법 개발 및 저항성 특성

Soo Min Lee1
,
Yong Ho Choi1
,
Jin Ju Lee1
,
Hun Kim1
,
Gyung Ja Choi1*
이 수민1
,
최 용호1
,
이 진주1
,
김 헌1
,
최 경자1*
1Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea
1한국화학연구원 친환경신물질연구센터
*Corresponding Author
28 February 2022. pp. 94-108
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Abstract

Black spot caused by Alternaria brassicicola is one of the most common and destructive diseases of brassica crops worldwide. This study was conducted to establish an efficient method for Chinese cabbage cultivars that are resistant to A. brassicicola and to define characteristics of resistant Chinese cabbage to A. brassicicola. First, we tested virulence of seven A. brassicicola isolates in three susceptible cultivars and selected KACC 40036, a moderately virulent isolate. Then, we tested the resistance degree of 87 commercial Chinese cabbage cultivars. For further study, we selected eight cultivars that showed different disease responses to the fungus. Development of black spot on the eight cultivars was investigated according to the growth stage of Chinese cabbage seedlings, inoculum concentration, and incubation temperature of inoculated plants. On the basis of these results, we suggest an efficient screening method for Chinese cabbage resistant to A. brassicicola: inoculate 16-day-old seedlings (fully expanded two-leaf stage) with a spore suspension at a concentration of 3.0 × 105 spores/mL using spray method and incubate the inoculated plant in a humidity chamber at 25°C for 24 hours and then transfer to a growth chamber at 25°C with 80% RH under 12-hour light per day. In addition, we selected 20 Chinese cabbage cultivars, resistant and weakly resistant to A. brassicicola KACC 40036, from the 87 cultivars. The occurrence of black spot caused by seven isolates of A. brassicicola on the 20 cultivars was measured. Development of black spot in each cultivar was positively correlated with the virulence of seven A. brassicicola isolates. The results suggest that resistance of Chinese cabbage to A. brassicicola isolates is likely affected by the virulence of the pathogen, but not a resulted of race differentiation.

Keywords
black spot
breeding
disease resistance
race
virulence

Alternaria brasisicola에 의한 검은무늬병은 배추과 작물에 전세계적으로 발생하여 심각한 피해를 입히는 병해 중 하나이다. 본 연구는 배추 검은무늬병의 효과적인 병리검정법을 확립하고, 저항성 특성을 구명하기 위하여 실험하였다. 감수성 배추 3개 품종에서 A. brassicicola 7개 균주들의 병원력을 조사하였고, 이들 중 중간 정도의 병원력을 나타내는 A. brassicicola KACC 40036 균주를 사용하여 시판 중인 배추 87개 품종의 검은무늬병에 대한 저항성 정도를 조사하였다. 이들 결과로부터 저항성 정도가 다른 8개의 배추 품종을 선발하여, 배추 유묘의 생장 정도, 접종원 농도, 그리고 접종한 식물체의 습실처리 온도 및 시간에 따른 검은무늬병 발생을 조사하였다. 이상의 결과로부터 배추의 검은무늬병에 대한 저항성 정도를 조사하기 위한 방법으로, 배추 종자를 파종하고 온실(25 ± 5°C)에서 16일 동안 재배하여 2엽이 완전 전개된 배추 유묘에 A. brassicicola 포자현탁액(3.0 × 105 spores/mL)을 분무하여 접종하고, 접종한 식물을 25°C 습실상에서 24시간 동안 배양한 후에, 상대습도 80%의 생육상(25°C)으로 이동하여 하루에 12시간씩 광을 조사하면서 재배하는 것을 제안하고자 한다. 또한 A. brassicicola에 대한 저항성 품종들의 저항성 특성을 조사하기 위하여, 실험한 87개 품종 중 검은무늬병 발생이 적었던 20개 품종을 선발하였다. 그리고 이들 품종에서의 A. brassicicola 7개 균주에 의한 검은무늬병 발생을 조사한 결과, 모든 품종들은 A. brassicicola 균주들의 병원력에 비례하여 검은무늬병이 발생하였다. 이들 결과는 배추의 A. brassicicola에 대한 저항성은 균주에 대하여 특이적이지 않고, 균주의 병원력에 영향을 받는다고 생각되었다.

키워드
검은무늬병
육종
병 저항성
레이스
병원력
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 40
  • No :1
  • Pages :94-108
  • Received Date : 2021-04-06
  • Revised Date : 2021-06-04
  • Accepted Date : 2021-07-13
  • DOI :https://doi.org/10.7235/HORT.20220010
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