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2026 Vol.44, Issue 2 Preview Page

Review

Optimization of Induction and Culture Strategies for Calli with Stem Cell-like Features for the Production of Functional Natural Products from Korean Native Plants: Case Studies Involving Artemisia, Ixeridium, and Perilla
Seon Hwa Lee1
,
Kang Mo Ku2
,
Ju Bin Cho2*
,
Woo Suk Cho1*
1Department of Agriculture Biotechnology, National Institute of Agricultural Science, RDA, Jeonju 54874, Korea
2Department of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea
*Corresponding Author
30 April 2026. pp. 161-178
PDF XML Citation
Abstract

Plant stem cells and calli with stem cell-like features provide scalable, sustainable platforms with superior genetic stability, reduced aggregation, and improved yield consistency compared to dedifferentiated cells (DDCs). We implemented a four-step workflow—induction, biochemical and ultrastructural validation, suspension-culture optimization, and LC–MS/MS quantification—spanning three Korean native species: in A. capillaris, root cells generated from calli with stem cell-like features exhibited approximately fourfold higher artemisinin content than intact plants; in I. dentatum, calli with stem cell-like features demonstrated approximately tenfold greater chrysoeriol-7-O-glucoside accumulation and secreted more than twofold more of this metabolite into the medium than was present intracellularly; and in P. frutescens, calli with stem cell-like features yielded approximately threefold more rosmarinic acid compared to DDCs (40.4 ± 1.4 vs. 12.9 ± 0.7 mg·g-1). At present, the integration of single-cell and spatial omics, elicitation, and advancements in media and scale-up engineering with AI technologies (ML soft sensors, digital twins, hybrid models) supports real-time process monitoring and control, minimizing variability and facilitating lab-to-industry translation. Future priorities include the development of robust stemness markers, standardized sensing strategies enabled by cross-species–generalizable AI, and long-term bioreactor reliability to achieve consistent, large-scale, and eco-friendly production of natural products using systems based on CMC- and calli with stem cell–like features.

Keywords
cambial meristematic cells (CMCs)
catalase activity
large-scale production
liquid condition culture
solid culture condition
transmission electron microscopy (TEM)
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 44
  • No :2
  • Pages :161-178
  • Received Date : 2025-08-07
  • Revised Date : 2025-10-14
  • Accepted Date : 2025-12-09
  • DOI :https://doi.org/10.7235/HORT.20250081
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