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2022 Vol.40, Issue 2 Preview Page

Research Article

Development of a CAPS Marker for Discriminating Sex in Actinidia arguta
Jung Kim1†
,
Sewon Oh1†
,
Yumi Kim1
,
Mockhee Lee2
,
Yong-Bum Kwack3
,
Daeil Kim1*
1Department of Horticulture, Chungbuk National University, Cheongju 28644, Korea
2Namhae Branch, National Institute of Horticultural and Herbal Science, Namhae 52430, Korea
3Department of Fruit Science, Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea
*Corresponding Author
†These authors contributed equally to this work.
30 April 2022. pp. 233-241
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Abstract

Actinidia arguta is a Korean native kiwifruit with valuable traits for kiwifruit breeding, such as cold hardiness, disease tolerance, and high nutritional value. Early sex discrimination is a priority in kiwifruit breeding programs because Actinidia species are dioecious vines. A cleaved amplified polymorphic sequence (CAPS) marker discriminating sex was developed using genotyping-by- sequencing (GBS)-derived single nucleotide polymorphisms (SNPs). Previously obtained GBS reads were re-aligned to the kiwifruit reference genome organized at the pseudo-chromosomal level. These GBS-SNPs could divide A. arguta accessions according to sex. Candidate CAPS markers were designed using these SNPs located on sex chromosomes. Among them, only the CAPS marker designed using the SNP located at 10,236,410 bp on sex chromosome was able to discriminate the sex of A. arguta accessions. This might be because the SNP located at 10,236,410 bp had higher read depth than other candidate SNPs. This CAPS marker, named CBk25ca01, produced DNA fragments of 300 and 147/153 bp in female and male A. arguta accessions, respectively. The CBk25ca01 marker will be useful to improve kiwifruit breeding programs by reducing the time needed to discriminate sex.

Keywords
kiwifruit
marker-assisted selection
polymorphism
restriction enzyme
single nucleotide polymorphism
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 40
  • No :2
  • Pages :233-241
  • Received Date : 2022-02-11
  • Revised Date : 2022-03-07
  • Accepted Date : 2022-03-12
  • DOI :https://doi.org/10.7235/HORT.20220022
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