Effect of Hydropriming and Light Quality Treatments on Sprout Growth and Antioxidant Activity in Brassica oleracea var. capitate Seeds

Hamin Lee; Boran Ji; Bo-Kook Jang; Kyungtae Park; Sang Yeob Lee; Kyunggu An; Ju-Sung Cho

doi:10.7235/HORT.20220023

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2022 Vol.40, Issue 2 Preview Page

Research Article

Effect of Hydropriming and Light Quality Treatments on Sprout Growth and Antioxidant Activity in Brassica oleracea var. capitate Seeds

30 April 2022. pp. 242-252

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Abstract

In this study, we assessed the effect of hydropriming and light quality treatments on the antioxidant activity and bioactive compounds in Brassica oleracea var. capitate sprouts. B. oleracea seeds were soaked in distilled water and treated with different light wavelengths or in complete darkness for 24 h at 20 ± 1°C. Subsequently, the treated seeds were dried for 24 h, sown on sprouting trays, and then incubated for seven days at 25 ± 2°C under a 16/8 h light/dark photoperiod with white LEDs. The final percent germination of B. oleracea sprouts was 82.3–95.7%. The fresh weight and shoot length increased significantly in all hydropriming and light quality treatments when compared to the control (soaked in water for 24 h in darkness with no drying). We tested different solvents for extracting bioactive compounds and determined their radical scavenging activity and found that 80% ethanol was the best solvent. Hydro-primed sprouts exhibited significant differences in bioactive compounds and antioxidant activity between the different light treatments. In particular, the blue light treatment resulted in a total flavonoid content and DPPH radical scavenging activity (RC₅₀) of 157.2 mg naringin equivalent (NE)·g^-1 and 1.27 mg·mL^-1, respectively, and was the most effective treatment for increasing bioactive compounds and antioxidant activity. Therefore, the combined germination pretreatments of hydropriming and light quality induced significant changes in bioactive compounds and antioxidant activity of B. oleracea sprouts.

Keywords

bioactive

extraction

flavonoid

free-radical

polyphenol,

scavenging

References

Acharya J, Rechner O, Neugart S, Schreiner M, Poehling HM (2016) Effects of light-emitting diode treatments on Brevicoryne brassicae performance mediated by secondary metabolites in Brussels sprouts. J Plant Dis Prot 123:321-330. doi:10.1007/s41348-016-0029-9 10.1007/s41348-016-0029-9

Adhikari B, Dhital PR, Ranabhat S, Poudel H (2021) Effect of seed hydro-priming durations on germination and seedling growth of bitter gourd (Momordica charantia). PLoS ONE 16:e0255258. doi:10.1371/journal.pone.0255258 10.1371/journal.pone.025525834351950PMC8341585

Ajouri A, Asgedom H, Becker M (2004) Seed priming enhances germination and seedling growth of barley under conditions of P and Zn deficiency. J Plant Nutr Soil Sci1 67:630-636. doi:10.1002/jpln.200420425 10.1002/jpln.200420425

Bendary E, Francis RR, Ali HMG, Sarwat MI, Hady El (2013) Antioxidant and structure-activity relationships (SARs) of some phenolic and anilines compounds. Ann Agric Sci 58:173-181. doi:10.1016/j.aoas.2013.07.002 10.1016/j.aoas.2013.07.002

Bian Z, Cheng R, Wang Y, Yang Q, Lu C (2018) Effect of green light on nitrate reduction and edible quality of hydroponically grown lettuce (Lactuca sativa L.) under short-term continuous light from red and blue light-emitting diodes. Environ Exp Bot 153:63-71. doi:10.1016/j.envexpbot.2018.05.010 10.1016/j.envexpbot.2018.05.010

Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200. doi:10.1038/1811199a0 10.1038/1811199a0

Choi J, Kim J, Yoon HI, Son JE (2022) Effect of far-red and UV-B light on the growth and ginsenoside content of ginseng (Panax ginseng CA Meyer) sprouts aeroponically grown in plant factories. Hortic Environ Biotechnol 63:77-87 doi:10.1007/s13580-021-00380-9 10.1007/s13580-021-00380-9

Ciska E, Honke J, Kozłowska H (2008) Effect of light conditions on the contents of glucosinolates in germinating seeds of white mustard, red radish, white radish, and rapeseed. J Agric Food Chem 56:9087-9093. doi:10.1021/jf801206g 10.1021/jf801206g18771273

Di Girolamo G, Barbanti L (2012) Treatment conditions and biochemical processes influencing seed priming effectiveness. Ital J Agron 7:e25. doi:10.4081/ija.2012.e25 10.4081/ija.2012.e25

Farooq M, Basra SM, Wahid A, Ahmad N (2010) Changes in nutrient-homeostasis and reserves metabolism during rice seed priming: consequences for seedling emergence and growth. Agric Sci China 9:191-198. doi:10.1016/S1671-2927(09)60083-3 10.1016/S1671-2927(09)60083-3

Goli AH, Barzegar M, Sahari MA (2005) Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chem 92:521-525. doi:10.1016/j.foodchem.2004.08.020 10.1016/j.foodchem.2004.08.020

Gragaham HD (1992) Modified prussian blue assay for total phenolic compound. J Agric Food Chem 40:801-805. doi:10.1021/jf00017a018 10.1021/jf00017a018

Haghighi M, Saadat S, Abbey L (2020) Effect of exogenous amino acids application on growth and nutritional value of cabbage under drought stress. Sci Hortic 272:109561. doi:10.1016/j.scienta.2020.109561 10.1016/j.scienta.2020.109561

International Seed Testing Association (ISTA) (2018) International rules for seed testing. ISTA, Switzerland, Zürich

Kadomura-Ishikawa Y, Miyawaki K, Noji S, Takahashi A (2013) Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria × ananassa fruits. J Plant Res 126:847-857. doi:10.1007/s10265-013-0582-2 10.1007/s10265-013-0582-223982948

Kim KC, Kim JS (2020) Effect of varying ethanol concentrations on the extraction properties and physiological activity of Artemisia annua L. Korean J Food Sci Technol 52:130-137. doi:10.9721/KJFST.2020.52.2.130

Kim SL, Kim SK, Park CH (2004) Introduction and nutritional evaluation of buckwheat sprouts as a new vegetable. Food Res Int 37:319-327. doi:10.1016/j.foodres.2003.12.008 10.1016/j.foodres.2003.12.008

Lee JH, Oh MM (2021) Growth and bioactive compound contents of various sprouts cultivated under dark and light conditions. J Bio-Env Con 30:218-229. doi:10.12791/KSBEC.2021.30.3.218 10.12791/KSBEC.2021.30.3.218

Liu H, Chen Y, Hu T, Zhang S, Zhang Y, Zhao T, Kang Y (2016) The influence of light-emitting diodes on the phenolic compounds and antioxidant activities in pea sprouts. J Funct Food 25:459-465. doi:10.1016/j.jff.2016.06.028 10.1016/j.jff.2016.06.028

Nam TG, Kim DO, Eom SH (2018) Effects of light sources on major flavonoids and antioxidant activity in common buckwheat sprouts. Food Sci Biotechnol 27:169-176. doi:10.1007/s10068-017-0204-1 10.1007/s10068-017-0204-130263737PMC6049769

Nawaz H, Shad MA, Rauf A (2018) Optimization of extraction yield and antioxidant properties of Brassica oleracea convar. capitata var L. leaf extracts. Food Chem 242:182-187. doi:10.1016/j.foodchem.2017.09.041 10.1016/j.foodchem.2017.09.04129037676

Neamatollahi E, Bannayan M, Darban AS, Ghanbari A (2009) Hydropriming and osmopriming effects on cumin (Cuminum cyminum L.) seeds germination. World Acad Eng 57:526-529

Novotny C, Schulzova V, Krmela A, Hajslova J, Svobodova K, Koudela M (2018) Ascorbic acid and glucosinolate levels in new Czech cabbage cultivars: effect of production system and fungal infection. Molecules 23:1855. doi:10.3390/molecules23081855 10.3390/molecules2308185530046026PMC6222616

Park CH, Kim NS, Park JS, Lee SY, Lee JW, Park SU (2019) Effects of light-emitting diodes on the accumulation of glucosinolates and phenolic compounds in sprouting canola (Brassica napus L.). Foods 8:76. doi:10.3390/foods8020076 10.3390/foods802007630791403PMC6406741

Peng L, Zou L, Su Y, Fan Y, Zhao G (2015) Effects of light on growth, levels of anthocyanin, concentration of metabolites in Fagopyrum tataricum sprout cultures. Int J Food Sci 50:1382-1389. doi:10.1111/ijfs.12780 10.1111/ijfs.12780

Pérez-Balibrea S, Moreno DA, García-Viguera C (2008) Influence of light on health-promoting phytochemicals of broccoli sprouts. J Sci Food Agric 88:904-910. doi:10.1002/jsfa.3169 10.1002/jsfa.3169

Podsędek A (2007) Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. Lebenson Wiss Technol 40:1-11. doi:10.1016/j.lwt.2005.07.023 10.1016/j.lwt.2005.07.023

Pukacka S, Ratajczak E (2005) Production and scavenging of reactive oxygen species in Fagus sylvatica seeds during storage at varied temperature and humidity. J Plant Physiol 162:873-885. doi:10.1016/j.jplph.2004.10.012 10.1016/j.jplph.2004.10.01216146313

Qian H, Liu T, Deng M, Miao H, Cai C, Shen W, Wang Q (2016) Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts. Food Chem 196:1232-1238. doi:10.1016/j.foodchem.2015.10.055 10.1016/j.foodchem.2015.10.05526593611

Razali N, Mat-Junit S, Abdul-Muthalib AF, Subramaniam S, Abdul-Aziz A (2012) Effects of various solvents on the extraction of antioxidant phenolics from the leaves, seeds, veins and skins of Tamarindus indica L. Food Chem 131:441-448. doi:10.1016/j.foodchem.2011.09.001 10.1016/j.foodchem.2011.09.001

Re R, Pellegrini N, Proteggente A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237. doi:10.1016/S0891-5849(98)00315-3 10.1016/S0891-5849(98)00315-3

Ryu JH, Kim RJ, Lee SJ, Kim IS, Lee HJ, Sung NJ (2011) Nutritional properties and biological activities of Artemisia annua L. J. Korean Soc Food Sci Nutr 40:163-170. doi:10.3746/jkfn.2011.40.2.163 10.3746/jkfn.2011.40.2.163

Samuolienė G, Viršilė A, Brazaitytė A, Jankauskienė J, Sakalauskienė S, Vaštakaitė V, Novi čkovas A, Sasnauskas A, Duchovskis P (2017) Blue light dosage affects carotenoids and tocopherols in microgreens. Food Chem 228:50-56. doi:10.1016/j.foodchem.2012.03.061 10.1016/j.foodchem.2012.03.06125005972

Sher A, Sarwar T, Nawaz A, Ijaz M, Sattar A, Ahmad S (2019) Methods of seed priming. In Hasanuzzaman, M, Fotopoulous V, eds, Priming and Pretreatment of Seeds and Seedlings. Springer, Singapore, pp 1-10. doi:10.1007/978-981-13-8625-1_1 10.1007/978-981-13-8625-1_130835513

Shi L, Cao S, Chen W, Yang Z (2014) Blue light induced anthocyanin accumulation and expression of associated genes in Chinese bayberry fruit. Sci Hortic 179:98-102. doi:10.1016/j.scienta.2014.09.022 10.1016/j.scienta.2014.09.022

Shin SL, Lee CH (2011) Antioxidant activities of ostrich fern by different extraction methods and solvents. J Life Sci 21:56-61. doi:10.5352/JLS.2011.21.1.56 10.5352/JLS.2011.21.1.56

Smith HL, McAusland L, Murchie EH (2017) Don't ignore the green light: exploring diverse roles in plant processes. J Exp Bot 68:2099-2110. doi:10.1093/jxb/erx098 10.1093/jxb/erx09828575474

Taulavuori K, Hyöky V, Oksanen J, Taulavuori E, Julkunen-Tiitto R (2016) Species-specific differences in synthesis of flavonoids and phenolic acids under increasing periods of enhanced blue light. Environ Exp Bot 121:145-150. doi:10.1016/j.envexpbot.2015.04.002 10.1016/j.envexpbot.2015.04.002

Thwe AA, Kim YB, Li X, Seo JM, Kim SJ, Suzuki T, Chung S-O, Park SU (2014) Effects of light-emitting diodes on expression of phenylpropanoid biosynthetic genes and accumulation of phenylpropanoids in Fagopyrum tataricum sprouts. J Agric Food Chem 62:4839-4845. doi:10.1021/jf501335q 10.1021/jf501335q24793050

Tsurunaga Y, Takahashi T, Katsube T, Kudo A, Kuramitsu O, Ishiwata M, Matsumoto S (2013) Effects of UV-B irradiation on the levels of anthocyanin, rutin and radical scavenging activity of buckwheat sprouts. Food Chem 141:552-556. doi:10.1016/j.foodchem.2013.03.032 10.1016/j.foodchem.2013.03.03223768393

Turkmen N, Sari F, Velioglu YS (2006) Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem 99:835-841. doi:10.1016/j.foodchem.2005.08.034 10.1016/j.foodchem.2005.08.034

Velioglu Y, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113-4117. doi:10.1021/jf9801973 10.1021/jf9801973

Waqas M, Korres NE, Khan MD, Nizami AS, Deeba F, Ali I, Hussain H (2019) Advances in the concept and methods of seed priming. In Hasanuzzaman, M, Fotopoulous V, eds, Priming and Pretreatment of Seeds and Seedlings. Springer, Singapore, pp 11-41. doi:10.1007/978-981-13-8625-1_2 10.1007/978-981-13-8625-1_2

Wu MC, Hou CY, Jiang CM, Wang YT, Wang CY, Chen HH, Chang HM (2007) A novel approach of LED light radiation improves the antioxidant activity of pea seedlings. Food Chem 101:1753-1758. doi:10.1016/j.foodchem.2006.02.010 10.1016/j.foodchem.2006.02.010

Xiao J, Capanoglu E, Jassbi AR, Miron A (2016) Advance on the flavonoid C-glycosides and health benefits. Crit Rev Food Sci Nutr 56:S29-S45. doi:10.1080/10408398.2015.1067595 10.1080/10408398.2015.106759526462718

Xu Y, Xiao Y, Lagnika C, Li D, Liu C, Jiang N, Song J, Zhang M (2020) A comparative evaluation of nutritional properties, antioxidant capacity and physical characteristics of cabbage (Brassica oleracea var. capitate var L.) subjected to different drying methods. Food Chem 309:124935. doi:10.1016/j.foodchem.2019.06.002 10.1016/j.foodchem.2019.06.00231732250

Zhang X, Bian Z, Yuan X, Chen X, Lu C (2020) A review on the effects of light-emitting diode (LED) light on the nutrients of sprouts and microgreens. Trends Food Sci Technol 99:203-216. doi:10.1016/j.tifs.2020.02.031 10.1016/j.tifs.2020.02.031

Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 40
No :2
Pages :242-252
Received Date : 2021-12-06
Revised Date : 2022-03-15
Accepted Date : 2022-03-21
DOI :https://doi.org/10.7235/HORT.20220023

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

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