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2025 Vol.43, Issue 5 Preview Page

Research Article

Effects of Different Temperature Conditions on the Growth and Photosynthesis of Korean Angelica Plug Seedlings in Plant Factories with Artificial Lighting Systems
Eun Won Park1
,
Jeong Hun Hwang1
,
Hee Sung Hwang23
,
Hyeong Eun Choi1
,
Jeong Kil Koo1
,
Ji Hye Yun1
,
So Yeong Hwang1
,
Seung Jae Hwang12345*
1Department of Applied Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Korea
2Division of Crop Science, Graduate School of Gyeongsang National University, Jinju 52828, Korea
3Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Korea
4Division of Horticultural Science, College of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea
5Reserch Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea
*Corresponding Author
31 October 2025. pp. 582-592
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Abstract

In this study, we aimed to evaluate the growth characteristics of Korean angelica (Angelica gigas Nakai) seedlings grown under different temperatures in plant factories with artificial lighting systems (PFALs). The temperatures in the PFALs were maintained at 22/19°C (light/dark), 25/22°C, and 28/25°C, with an alternating 12-hour photoperiod used in each case. Light-emitting diodes (red : green : blue = 7 : 1 : 2) were used as the light sources. Before sowing, Korean angelica seeds underwent two weeks of a cold treatment to break their morphophysiological dormancy. Seeds were sown in 72-cell plug trays, and the resultant seedlings were grown for 60 days to evaluate their growth and development. Additionally, principal component analysis (PCA) was employed to examine the relationships between shoot and root development. It was found that the shoot growth parameters of the crown diameter, leaf area, and leaf dry weight increased at 22/19°C and 25/22°C compared to 28/25°C. Seedlings grown at 22/19°C showed a larger root volume and a significantly higher root : shoot ratio, which was more effective for root development. The net photosynthesis rate tended to increase with a decrease in the temperature. The PCA results confirmed a strong correlation among the petiole length, crown and root diameters, leaf area, leaf and root dry weights, and root volume. These results indicate that Korean angelica seedlings exhibit enhanced root development and net photosynthesis at relatively low temperatures in PFALs.

Keywords
Angelica gigas Nakai
leaf gas exchange
principal component analysis
root volume
transpiration
References
1

Ali MM, Al-Ani A, Eamus D, Tan DKY (2017) Leaf nitrogen determination using non-destructive techniques-A review. J Plant Nutr 40:928-953. https://doi.org/10.1080/01904167.2016.1143954

10.1080/01904167.2016.1143954
2

Baskin CC, Baskin JM (2014) Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination, 2nd edn. Elsevier/Academic Press, San Diego, CA, USA, pp 10-303

3

Beadle CL (1993) Growth analysis. In DO Hall, ed, Photosynthesis and Production in a Changing Environment: A Field and Laboratory Manual. Chapman & Hall, London, UK, pp 36-46

10.1007/978-94-011-1566-7_3
4

Carlson TN, Ripley DA (1997) On the relation between NDVI, fractional vegetation cover, and leaf area index. Remote Sens Environ 62:241-252. https://doi.org/10.1016/S0034-4257(97)00104-1

10.1016/S0034-4257(97)00104-1
5

Choi HE, Hwang SY, Yun JH, Yu J, Hwang JH, Park EW, Koo JK, Hwang HS, Hwang SJ (2023) Growth and seedling quality of grafted cucumber seedlings by different cultivars and supplemental light sources of low radiation period and early yield of cucumber after transplanting. J Bio-Env Con 32:319-327. https://doi.org/10.12791/KSBEC.2023.32.4.319

10.12791/KSBEC.2023.32.4.319
6

Dutta S, Mohanty S, Tripathy B (2009) Role of temperature stress on chloroplast biogenesis and protein import in pea. Plant Physiol 150:1050-1061. https://doi.org/10.1104/pp.109.137265

10.1104/pp.109.13726519403728PMC2689951
7

Giri A, Heckathorn S, Mishra S, Krause C (2017) Heat stress decreases levels of nutrient-uptake and-assimilation proteins in tomato roots. Plants 6:6. https://doi.org/10.3390/plants6010006

10.3390/plants601000628106834PMC5371765
8

Hasanuzzaman M, Nahar K, Alam MM, Roychowdhury R, Fujita M (2013) Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J Mol Sci 14:9643-9684. https://doi.org/10.3390/ijms14059643

10.3390/ijms1405964323644891PMC3676804
9

Hatfield JL, Boote KJ, Kimball BA, Ziska LH, Izaurralde RC, Ort D, Thomson AM, Wolfe D (2011) Climate impacts on agriculture: implications for crop production. Agron J 103:351-370. https://doi.org/10.2134/agronj2010.0303

10.2134/agronj2010.0303
10

Hatfield JL, Prueger JH (2015) Temperature extremes: Effect on plant growth and development. Weather Clim Extrem 10:4-10. https://doi.org/10.1016/J.WACE.2015.08.001

10.1016/j.wace.2015.08.001
11

He Z, Wang Y, Chen Y, Geng F, Jiang Z, Li X (2023) Angelica gigas Nakai: An overview on its chemical composition and pharmacological activity. Biochem Syst Ecol 111:104717. https://doi.org/10.1016/J.BSE.2023.104717

10.1016/j.bse.2023.104717
12

Hwang SY, Yun JH, Yu J, Hang JH, Park EW, Jeong HW, Hwang HS, Hwang SJ (2024) Proper day and night temperatures for different cultivars of cucumber seedlings after the grafting stage. Hortic Sci Technol 42:492-503. https://doi.org/10.7235/HORT.20240031

10.7235/HORT.20240031
13

Jackson TJ, Chen D, Cosh M, Li F, Anderson M, Walthall C, Doriaswamy P, Hunt ER (2004) Vegetation water content mapping using Landsat data derived normalized difference water index for corn and soybeans. Remote Sens Environ 92:475-482. https://doi.org/10.1016/j.rse.2003.10.021

10.1016/j.rse.2003.10.021
14

Jang I, Lee B, Kim J, Hwang H, Cho H, Ryu H, Chun C (2024) Thermophysiological responses of ginseng to abnormal season-long high temperature. Hortic Environ Biotechnol 65:761-770. https://doi.org/10.1007/s13580-024-00603-9

10.1007/s13580-024-00603-9
15

Jeong DH, Kim KY, Park SH, Jung CR, Jeon KS, Park HW (2021) Growth and useful component of Angelica gigas Nakai under high temperature stress. Korean J Plant Res 34:287-296. https://doi.org/10.7732/kjpr.2021.34.4.287

10.7732/kjpr.2021.34.4.287
16

Jeong SY, Kim HM, Lee KH, Kim KY, Huang DS, Kim JH, Seong RS (2015) Quantitative analysis of marker compounds in Angelica gigas, Angelica sinensis, and Angelica acutiloba by HPLC/DAD. Chem Pharm Bull 63:504-511. https://doi.org/10.1248/cpb.c15-00081

10.1248/cpb.c15-0008125946978
17

Ju J, Zhang S, Hu Y, Zhang M, He R, Li Y, Liu X, Liu H (2023) Effects of supplemental red and far-red light at different growth stages on the growth and nutritional properties of lettuce. Agronomy 14:55. https://doi.org/10.3390/agronomy14010055

10.3390/agronomy14010055
18

Kim J, Savin R, Slafer GA (2024a) Quantifying pre-and post-anthesis heat waves on grain number and grain weight of contrasting wheat cultivars. Field Crops Res 307:109264. https://doi.org/10.1016/j.fcr.2024.109264

10.1016/j.fcr.2024.109264
19

Kim JH, Doh EJ, Kim HY, Lee G (2024b) Chemical relationship among genetically authenticated medicinal species of Genus Angelica. Plants 13:1252. https://doi.org/10.3390/plants13091252

10.3390/plants1309125238732467PMC11085054
20

Kozai T (2013) Resource use efficiency of closed plant production system with artificial light: Concept, estimation and application to plant factory. Proc Jpn Acad Ser B 89:447-461. https://doi.org/10.2183/pjab.89.447

10.2183/pjab.89.44724334509PMC3881955
21

Kwak MJ, Kim Y, Lee J, Kim J, Kim E, Kang D, Choi W, Woo SY (2024) Temperature-mediated alterations in the growth, physiology, morphology, and pharmacology of Astragalus membranaceus Bunge: implications for medicinal herb cultivation and therapeutic efficacy. Hortic Sci Technol 42:433-451. https://doi.org/10.7235/HORT.20240036

10.7235/HORT.20240036
22

Lee HS, Han JE, Bae EK, Jie EY, Kim SW, Kwon HJ, Lee HS, Yeon SH, Murthy HN, et al. (2024) Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica gigas Nakai. BMC Plant Biol 24. https://doi.org/10.1186/s12870-024-05243-w

10.1186/s12870-024-05243-w38858674PMC11165744
23

Lee JY, Hiyama M, Hikosaka S, Goto E (2020) Effects of concentration and temperature of nutrient solution on growth and camptothecin accumulation of Ophiorrhiza pumila. Plants 9:793. https://doi.org/10.3390/plants9060793

10.3390/plants906079332630386PMC7355462
24

Liu B, Zhao D, Zhang P, Liu F, Jia M, Liang J (2020) Seedling evaluation of six walnut rootstock species originated in China based on principal component analysis and cluster analysis. Sci Hortic 265:109212. https://doi.org/10.1016/J.SCIENTA.2020.109212

10.1016/j.scienta.2020.109212
25

Macías-Rodríguez L, Quero E, López MG (2002) Carbohydrate differences in strawberry crowns and fruit (Fragaria × ananassa) during plant development. J Agric Food Chem 50:3317-3321. https://doi.org/10.1021/jf011491p

10.1021/jf011491p12010004
26

Ministry of Agriculture, Food and Rural Affairs (MAFRA) (2024) 2023 Special Crop Production Statistics. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea, pp 36-37

27

Onwuka B, Mang B (2018) Effects of soil temperature on some soil properties and plant growth. Adv Plants Agric Res 8:34-37. https://doi.org/10.15406/apar.2018.08.00288

10.15406/apar.2018.08.00288
28

Park EW, Hwang JH, Hwang HS, Hwang SY, Yu J, Lee ES, Hwang SJ (2023) Germination characteristics of Angelica gigas and Angelica acutiloba as affected by cold stratification and hot water treatment before sowing. In IV Asian Horticultural Congress-AHC2023 1404. pp 505-512. https://doi.org/10.17660/ActaHortic.2024.1404.68

10.17660/ActaHortic.2024.1404.68
29

Park EW, Hwang JH, Hwang HS, Yun JH, Hwang SY, Yu J, Choi HE, Koo JK, Lee ES, et al. (2025) Effect of temperature and seedling cultivation period on the quality of plug seedlings of four medicinal plant. Hortic Environ Biotechnol 1-12. https://doi.org/10.1007/s13580-024-00659-7

10.1007/s13580-024-00659-7
30

Rural Development Administration (RDA) (2018) Korean Angelica Root. RDA, Jeonju, Korea, pp 18-77

31

Wang L, Dang QL (2023) CO2 demand-supply coordination in photosynthesis reflecting the plant-environment interaction: extension and parameterization of demand function and supply function. Am J Plant Sci 14:220-245. https://doi.org/10.4236/ajps.2023.142017

10.4236/ajps.2023.142017
32

Yamaura H, Kanno K, Iwasaki Y, Nakano A, Isozaki M (2023) Controlling growth and carbohydrate utilization of tomato seedlings through day-night temperature difference and high light intensity under elevated CO2 conditions. Sci Hortic 322:112427. https://doi.org/10.1016/J.SCIENTA.2023.112427

10.1016/j.scienta.2023.112427
33

Yeom MS, Oh MM (2023) Air and root zone temperature for growth of Coastal Glehnia seedlings. Hortic Sci Technol 41:177-187. https://doi.org/10.7235/HORT.20230017

10.7235/HORT.20230017
34

Yun HB, Yang HC, Kim YH, Hyeon SJ, Choi MS, Vu N, Jang DC (2025) Seedling quality and early growth after the transplanting of tomato seedlings grown using different production methods. Hortic Sci Technol 43:1-11. https://doi.org/10.7235/HORT.20250025

10.7235/HORT.20250025
35

Yun YB, Koo HJ, Kwon HY, Song Y, Lee KC, Um Y (2024) Effects of CO2 concentration and temperature on growth characteristics, physiological activities, and antioxidant activities of Angelica gigas Nakai. J Plant Biol 67:1-20. https://doi.org/10.1007/s12374-024-09437-5

10.1007/s12374-024-09437-5
36

Zhang H, Zhu J, Gong Z, Zhu J (2022) Abiotic stress responses in plants. Nat Rev Genet 23:104-119. https://doi.org/10.1038/s41576-021-00413-0

10.1038/s41576-021-00413-034561623
37

Zhang L, Xu C, Liu H, Tao J, Zhang K (2023) Seed dormancy and germination requirements of Torilis scabra (Apiaceae). Agronomy 13:1250. https://doi.org/10.3390/agronomy13051250

10.3390/agronomy13051250
38

Zhao G, Pei Y, Yang R, Xiang L, Fang Z, Wang Y, Yin D, Wu J, Gao D, et al. (2022) A non-destructive testing method for early detection of ginseng root diseases using machine learning technologies based on leaf hyperspectral reflectance. Front Plant Sci 13:1031030. https://doi.org/10.3389/fpls.2022.1031030

10.3389/fpls.2022.103103036466253PMC9714554
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :5
  • Pages :582-592
  • Received Date : 2025-02-18
  • Revised Date : 2025-05-07
  • Accepted Date : 2025-05-16
  • DOI :https://doi.org/10.7235/HORT.20250053
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