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2025 Vol.43, Issue 2

Research Article

Development of Theanine Molecular Markers for High-Throughput Screening (HTS) in Camellia sinensis Breeding

차나무 분자육종 대량신속검색기술 적용을 위한 테아닌 분자마커 개발

Byung-Hyuk Kim1
,
Jong-Ok Jang1
,
Yun-Suk Kwon1
,
Yong Hee Kwon1
,
So Jin Lee1
,
Eun Yong Song1
,
Hyun-Hee Han1
,
Doo-Gyung Moon1*
김 병혁1
,
장 종옥1
,
권 윤숙1
,
권 용희1
,
이 소진1
,
송 은영1
,
한 현희1
,
문 두경1*
1Research Institute for Climate Change and Agriculture, National Institute of Horticultural & Herbal Science, Jeju 63240, Korea
1국립원예특작과학원, 온난화대응농업연구소
*Corresponding Author
30 April 2025. pp. 245-254
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Abstract

Tea is one of the most popular and widely consumed beverages in the world. Theanine is a very important amino acid in tea plants that is a component of green tea and contributes to the special flavor of the tea. It is very important to the breeding of tea plants for a special and excellent tea. However, traditional tea breeding is multistage a very long breeding process of 20–25 years. Therefore, this study was conducted to develop a High Throughput Screening System (HTS system) for the early selection of tea plants with high theanine content. We have developed molecular markers that can detect theanine synthetic gene (tsa gene) on tea breeding. We compared theanine quantitation and mRNA expression in Camellia sinensis under shading conditions. As a result, it was confirmed that gene expression and theanine synthesis were very close to each other in C. sinensis leaves. This technique could be utilized as an efficient method for early screening of genetic resources that produce high theanine based on molecular markers.

Keywords
gene
mRNA expression
probe
real-time PCR
tea
theanine synthesis

차는 범세계적으로 소비되는 인기있는 음료 중 하나이다. 테아닌은 차나무의 아미노산으로 알려져 있으며, 차의 특별한 풍미를 내는 매우 중요한 성분이다. 차나무 육종은 특별하고 우수한 차 생산을 위해 매우 중요하다. 그러나 전통적인 차나무 육종은 20–25년의 매우 길고 다단계의 육종과정을 필요로 한다. 따라서, 본 연구는 테아닌 함량이 높은 차나무의 조기선발을 위한 HTS 시스템(High Throughput Screening System) 개발을 목적으로 수행되었다. 차나무 육종과정에서 테아닌 합성 유전자(tsa gene)를 검출할 수 있는 분자마커를 개발했으며, 차광조건에서 테아닌 합성유전자의 발현량과 테아닌 함량을 분석하여 비교하였다. 그 결과 테아닌 합성유전자의 발현과 테아닌 함량의 상관관계가 매우 높음을 확인하였다. 따라서, 이 기술은 분자 마커를 기반으로 높은 테아닌을 생산하는 유전자원을 조기에 선별하는 효율적인 방법으로 유용하게 활용할 수 있을 것이다.

키워드
유전자
mRNA 발현
프루브
실시간유전자분석
차
테아닌 합성
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :2
  • Pages :245-254
  • Received Date : 2023-07-18
  • Revised Date : 2023-11-28
  • Accepted Date : 2024-02-06
  • DOI :https://doi.org/10.7235/HORT.20250003
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