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Research Article

Reflective Film Mulching Before Harvest Promotes Coloration and Expression of Ripening-Related Genes in Peach Fruits

수확 전 수관 하부 반사필름 처리에 의한 복숭아 과실의 착색 및 관련 유전자의 발현 촉진

Dan Bi Lee1
,
Gook Jin Lee1
,
Young Jun You1
,
Soon Young Ahn1
,
Hae Keun Yun1*
이 단비1
,
이 국진1
,
유 영준1
,
안 순영1
,
윤 해근1*
1Department of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Korea
1영남대학교 원예생명과학과
*Corresponding Author
30 June 2021. pp. 324-331
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Abstract

Fruit appearance is the most important factor of fruit quality in peach (Prunus persica). In order to determine the promotional effects of increased light in the canopy on peach fruit coloration, reflective film was applied under trees in the field 7 days before the harvest of ‘Taiko’ peach. We then investigated pigmentation of the peel, fruit traits like total soluble solids, titratable acidity, and hardness; the expression of genes related to fruit coloration and ripening in a peach fruits; and photosynthetically active radiation (PAR) inside the tree canopy treated with reflective film mulching. The color of fruit skins treated with reflective film mulching was improved significantly according to their Hunter value compared to untreated fruit. There was no significant change in total soluble solids, acid content, and hardness in fruits treated with reflective film mulching compared to control. Quantitative real-time PCR analysis revealed that transcript levels of anthocyanin biosynthetic related genes (F3H and CHS) were up-regulated; however, the expression of ethylene synthesis-related genes (ACO and ERS1) and ripening-related genes (PL and PME) was not enhanced by reflective film mulching. These results suggest that reflective film mulching improved light irradiance in the tree canopy, which promoted fruit skin coloration via the the induction of anthocyanin biosynthesis in the peel of peach.

Keywords
coloration
gene expression
peach
photosynthetically active radiation (PAR)
quantitative real-time PCR
reflective film

복숭아의 외관은 과실의 품질을 결정하는데 중요한 요인이다. 반사필름에 의한 복숭아 과피의 착색 촉진 효과를 구명하기 위하여, 복숭아(Prunus persica, 품종: Taiko) 과실을 수확 7일전에 과원의 수관 하부에 반사필름을 피복한 후, 수확한 과실의 착색, 가용성 고형물 함량(SSC), 적정산도(TA), 경도(N), 과실의 성숙 및 착색과 관련된 유전자의 발현량을 조사하였다. 반사필름이 처리된 수관 내부의 광투과량(광합성유효방사)이 증가하고, Hunter a값이 현저하게 상승되었다. 반사필름이 처리된 과실의 가용성 고형물 함량, 적정산도 및 경도에는 큰 변화가 없었다. 반사필름을 처리한 과실에서 안토시아닌 생합성과 관련된 유전자(CHS 및 F3H)의 발현은 유도되었으나, 에틸렌 합성에 관여하는 유전자(ACO 및 ERS1) 및 세포벽 연화에 연관된 유전자(PL 및 PME)의 발현은 증가하지 않았다. 이러한 결과는 복숭아 수체 하부의 반사필름 멀칭으로 수관 내부의 광환경을 개선하여 복숭아 과피에서 색소합성 관련 유전자의 발현을 유도하여 착색이 촉진되었음을 의미하는 것이다.

키워드
광합성유효방사
반사필름
복숭아
유전자 발현,
착색
Real-Time PCR
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :3
  • Pages :324-331
  • Received Date : 2020-11-06
  • Revised Date : 2020-12-15
  • Accepted Date : 2020-12-29
  • DOI :https://doi.org/10.7235/HORT.20210029
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