Accumulation of Cadmium and Lead in Four Cultivars of Radish (Raphanus sativus L.) during the Seedling Period

Yang Gyu Ku; Sung-Ju Ahn; Yeon-Ok Kim

doi:10.7235/HORT.20210028

All Issue

2021 Vol.39, Issue 3 Preview Page Next Page

Research Article

Accumulation of Cadmium and Lead in Four Cultivars of Radish (Raphanus sativus L.) during the Seedling Period

30 June 2021. pp. 314-323

PDF XML

Abstract

Cadmium (Cd) and lead (Pb) are toxic even in small quantities and decrease crop yields. They are easily absorbed by plant roots, and thus enter the food chain, risking human health. Radish (Raphanus sativus L.) is a widely consumed root vegetable in Korea. Therefore, it is important to screen for a radish cultivar that exhibits low Cd and Pb accumulation and elucidate the Cd and Pb tolerance mechanism. In this study, we determined the effect of Cd and Pb stress on germination and growth in four radish cultivars, ‘Gaulgeojang’ (AG), ‘Iseogaul’ (IG), ‘Chongryong’(CR), and ‘Supertogwang’ (ST). Furthermore, Cd and Pb concentrations, production of nonprotein thiols (NPTs), and hydrogen peroxide (H₂O₂) levels were investigated in four cultivars of radish seedlings under Cd and Pb treatments. The cultivars showed tolerance to Cd and Pb stress during germination and seedling growth in the following order: CR > ST > IG > AG. CR, a tolerant cultivar, accumulated less Cd in roots and less Pb in both roots and shoots than the other cultivars. In contrast, AG, a sensitive cultivar, exhibited greater Cd and Pb accumulation in roots than the other cultivars. Cd and Pb treatments significantly increased the NPTs in all four cultivars; the highest level was found in CR. Cd and Pb treatments also increased H₂O₂ levels in all cultivars; the highest and lowest levels were observed in AG and CR, respectively. These results indicate that the greater Cd and Pb tolerance of CR may be attributable to its higher potential to limit Cd and Pb accumulation and to form complexes of metal-binding ligands with Cd and Pb. The results of this study provide information for the selection of the safest of the four common radish cultivars for growth in Cd- and Pb-contaminated soils.

Keywords

contaminated soil

heavy metal accumulation

hydrogen peroxide

nonprotein thiols

sensitive cultivar

tolerant cultivar

References

Aggarwal A, Sharma I, Tripati BN, Munjal AK, Baunthiyal M, Sharma V (2012) Metal toxicity and photosynthesis. In: Photosynthesis: overviews on recent progress & future perspectives. 1st ed. New Delhi: IK International Publishing House Pvt. Ltd, pp 229-236

Ali H, Khan E, Sajad MA (2013) Phytoremediation of heavy metals-Concepts and applications. Chemosphere 91:869-881. doi:10.1016/j.chemosphere.2013.01.075 10.1016/j.chemosphere.2013.01.07523466085

Balkhair KS, Ashraf MA (2016) Field accumulation risks of heavy metals in soil and vegetable crop irrigated with sewage water in western region of Saudi Arabia. Saudi J Biol Sci 23:S32-S44. doi:10.1016/j.sjbs.2015.09.023 10.1016/j.sjbs.2015.09.02326858563PMC4705247

Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825-832. doi:10.1104/pp.123.3.825 10.1104/pp.123.3.82510889232PMC1539264

Dembitsky V, Rezanka T (2003) Natural occurrence of arseno compounds in plants, lichens, fungi, algal species, and microorganisms. Plant Sci 165:1177-1192. doi:10.1016/j.plantsci.2003.08.007 10.1016/j.plantsci.2003.08.007

Fargasova A (2001) Phytotoxic effects of Cd, Zn, Pb, Cu and Fe on Sinapis alba L. seedlings and their accumulation in roots and shoots. Biol Plant 44:471-473. doi:10.1023/A:1012456507827 10.1023/A:1012456507827

Flora SJS, Mittal M, Mehta A (2008) Heavy metal induced oxidative stress & its possible reversal by chelation therapy. Indian J Med Res 128:501-523

Gallego SM, Pena LB, Barcia RA, Azpilicueta CE, Iannone MF, Rosales EP, Zawoznik MS, Groppa MD, Benavides MP (2012) Unravelling cadmium toxicity and tolerance in plants: Insight into regulatory mechanisms. Environ Exp Bot 83:33-46. doi:10.1016/j.envexpbot.2012,04.006 10.1016/j.envexpbot.2012.04.006

Hasan MK, Cheng Y, Kanwar MK, Chu XY, Ahammed GJ, Qi ZY (2017) Responses of plant proteins to heavy metal stress-A review. Front Plant Sci 8:1492. doi:10.3389/fpls.2017.01492 10.3389/fpls.2017.0149228928754PMC5591867

Hossain MA, Piyatida P, Teixeira da Silva JA, Fujita M (2012) Molecular mechanism of heavy metal toxicity and tolerance in plants: Central role of glutathione in detoxification of reactive oxygen species and methylglyoxal and in heavy metal chelation. Hindawi Publishing Corporation J Bot 2012:1-37. doi:10.1155/2012/872875 10.1155/2012/872875

Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN (2014) Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol 7:60-72. doi:10.2478/intox-2014-0009 10.2478/intox-2014-000926109881PMC4427717

Kim YO, Jung S, Kim K, Bae HJ (2013) Role of pCeMT, a putative metallothionein from Colocasia esculenta, in response to metal stress. Plant Physiol Biochem 64:25-32. doi:10.1016/j.plaphy.2012.12.009 10.1016/j.plaphy.2012.12.00923344478

Lee YH, Lee JH, Kang HR, Ha JH, Lee BH, Kim SH (2015) A case of anaphylaxis induced by contact with young radish (Raphanus sativus L). Allergy Asthma Immunol Res 7:95-97. doi:10.4168/aair.2015.7.1.95 10.4168/aair.2015.7.1.9525553270PMC4274477

MacFarlane GR, Pulkownik A, Burchett MD (2003) Accumulation and distribution of heavy metals in the grey mangrove, Avicennia marina (Forsk.) Vierh.: biological indication potential. Environ Pollut 123:139-151. doi:10.1016/S0269-7491(02)00342-1 10.1016/S0269-7491(02)00342-1

Maksymiec W (2007) Signaling responses in plants to heavy metal stress. Acta Physiol Plant 29:177-187. doi:10.1007/s11738-007-0036-3 10.1007/s11738-007-0036-3

Malayeri BE, Chehregani A, Yousefi N, Lorestani B (2008) Identification of the hyper accumulator plants in copper and iron mine in Iran. Pak J Biol Sci 11:490-492. doi:10.3923/pjbs.2008.490.492 10.3923/pjbs.2008.490.49218817181

Mishra S, Dubey RS (2006) Heavy metal uptake and detoxification mechanisms in plants. Int J Agric Research 1:122-141. doi:10.3923/ijar.2006.122.141 10.3923/ijar.2006.122.141

Ro HM, Choi HJ, Yun SI, Park JS (2018) Organic amendment-driven removal and speciation of metals using wormwood in two contrasting soils near an abandoned copper mine. Hortic Environ Biotechnol 59:775-786. doi:10.1007/s13580-018-0073-4 10.1007/s13580-018-0073-4

Schmidt U (2003) Enhancing Phytoextraction: The effect of chemical soil manipulation on mobility, plant accumulation, and leaching of heavy metals. J Environ Qual 32:1939-1954. doi:10.2134/jeq2003.1939 10.2134/jeq2003.193914674516

Semhi K, Clauer N, Chaudhuri S (2014) Changing elemental uptake of radish seedlings grown in Cd and Pb polluted smectite substrates. Appl Clay Sci 99:171-177. doi:10.1016/j.clay.2014.06.029 10.1016/j.clay.2014.06.029

Seregin IV, Ivanov VB (2001) Physiological aspects of cadmium and lead toxic effects on higher plants. Russ J Plant Physiol 48:523-544. doi:10.1023/A:1016719901147 10.1023/A:1016719901147

Seregin IV, Shpigun LK, Ivanov VB (2004) Distribution and toxic effects of cadmium and lead on maize roots. Russ J Plant Physiol 51:525-533. doi:10.1023/B:RUPP.0000035747.42399.84 10.1023/B:RUPP.0000035747.42399.84

Sethy SK, Ghosh S (2013) Effect of heavy metals on germination of seeds. J Nat Sci Biol Med 4:272-275. doi:10.4103/0976-9668.116964 10.4103/0976-9668.11696424082715PMC3783763

Sneller FEC, van Heerwaarden LM, Koevoets PLM, Vooijs R, Schat H, Verkleij JAC (2000) Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: comparison of derivatization with Ellman's reagent and monobromobimane. J Agric Food Chem 48:4014-4019. doi:10.1021/jf9903105 10.1021/jf990310510995306

Storelli MM (2008) Potential human health risks from metals (Hg, Cd, and Pb) and polychlorinated biphenyls (PCBs) via seafood consumption: Estimation of target hazard quotients (THQs) and toxic equivalents (TEQs). Food Chem Toxicol 46:2782-2788. doi:10.1016/j.fct.2008.05.011 10.1016/j.fct.2008.05.01118584931

Tamás MJ, Sharma SK, Ibstedt S, Jacobson T, Christen P (2014) Heavy metals and metalloids as a cause for protein misfolding and aggregation. Biomolecules 4:252-267. doi:10.3390/biom4010252 10.3390/biom401025224970215PMC4030994

Xiao R, Wang S, Li R, Wang JJ, Zhang Z (2017) Soil heavy metal contamination and health risks associated with artisanal gold mining in Tongguan, Shaanxi, China. Ecotoxicol Environ Saf 141:17-24. doi:10.1016/j.ecoenv.2017.03.002 10.1016/j.ecoenv.2017.03.00228285205

Yadav SK (2010) Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. S Afr J Bot 76:167-179. doi:10.1016/j.sajb.2009.10.007 10.1016/j.sajb.2009.10.007

You J, Chan Z (2015) ROS Regulation during abiotic stress responses in crop plants. Front Plant Sci 6:1092. doi:10.3389/fpls.2015.01092 10.3389/fpls.2015.01092

Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 39
No :3
Pages :314-323
Received Date : 2020-10-15
Revised Date : 2020-12-23
Accepted Date : 2021-01-01
DOI :https://doi.org/10.7235/HORT.20210028

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

All Issue