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2024 Vol.42, Issue 4 Preview Page

Research Article

Collection and Morphological Analysis of Korean Wild Chrysanthemum zawadskii and C. makinoi

수집 야생 구절초 및 마키노 국화의 형태학적 세포학적 분석

Jeong So1
,
Youn Ok Jung2
,
Hak-Dong Lee34
,
Sanghyun Lee34
,
Jin Hee Lim1*
소 정1
,
정 연옥2
,
이 학동34
,
이 상현34
,
임 진희1*
1Department of Bio-Industry Resources Engineering, Sejong University, Seoul 05006, Korea
2SooY-K Biolab, Seongnam 13216, Korea
3Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea
4Natural Product Institute of Science and Technology, Anseong 17546, Korea
1세종대학교 생명시스템학부
2㈜수이케이
3중앙대학교 식물생명공학과
4한국천연물과학기술연구소
*Corresponding Author
31 August 2024. pp. 504-517
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Abstract

Over the course of seven years, different varieties of Chrysanthemum zawadskii were collected from 30 regions of Korea. All C. zawadskii varieties exhibit ray flowers that were either white or pink regardless of the collection region. However, they were classified into three groups according to the shape of the leaves, with large differences in the leaf shape (Group 1: C. zawadskii, Group 2: C. zawadskii with a slender leaf, and Group 3: C. zawadskii var. yezoense). A different species of Chrysanthemum called C. makinoi was assigned to Group 4. In terms of location, Group 1 can be found throughout Korea, while Group 2 is found primarily in highlands and confined locations such as Mt. Baekdu, Ulleung Island, and Mt. Halla on Jeju Island. Group 3 is found near the coast and has broad leaves with minimal splits, whereas Group 4 has been identified in Gangwon Province, a limestone location, with a variety of leaf shapes. One region was chosen for each group for an extensive comparison (Group 1: C. zawadskii var. latilobum from Mt. Hwangmae, Group 2: C. zawadskii var. lucidum from Ulleung Island, Group 3: C. zawadskii var. yezoense from Geoje Island, and Group 4: C. makinoi from Jeongseon). When morphological traits were compared by group, C. zawadskii var. lucidum (Group 2) had the widest flower diameter at 60.35 mm. Moreover, C. zawadskii (Group 1) and C. makinoi (Group 4) had the largest leaf length and width at the base, with no significant differences in the chlorophyll content. The cauline leaf of C. zawadskii var. yezoense (Group 3) had the greatest leaf length (38.79 mm), width (37.14 mm), and chlorophyll content (37.13 SPAD). However, the difference was not statistically significant when compared to the basal leaf. Ultimately, the results of this study showed that C. zawadskii var. latilobum (Group 1) and C. zawadskii var. yezoense (Group 3) were hexaploid, C. zawadskii var. lucidum (Group 2) was tetraploid, and C. makinoi (Group 4) was diploid. This research was carried out as part of a standardized procedure aimed at determining resources for use as basic data for Chrysanthemum genome research and functional breeding.

Keywords
chlorophyll content
chromosomal ploidy
functional breeding
industrial application
standardized procedure

7년에 걸쳐 한국의 30개 지역에 분포하고 있는 다양한 구절초들을 수집하였다. 모든 구절초들은 자생지와 무관하게 흰색 또는 분홍색 설상화 형태로 나타난다. 이와 달리, 잎 형태는 차이가 크므로 잎의 형태에 따라 3개 그룹으로 분류하였다(Group 1: 구절초, Group 2: 가는잎구절초, Group 3: 남구절초). Group 4는 마키노국화로 구절초와는 다른 국화 종이 분류되었다. 위치적으로 Group 1은 전국적으로 분포되었으며, Group 2는 주로 백두산, 울릉도, 제주도 한라산과 같이 상대적으로 고지대인 제한적인 지역에 서식한다. Group 3은 해안가에서 발견되며 잎이 넓고 갈라짐이 적은 반면, Group 4는 석회암 지대인 강원도에서 서식하며 잎 모양이 다양하다. 비교를 위해 각 group별로 한 지역을 선정하였다(Group 1: 황매산 자생 구절초, Group 2: 울릉국화, Group 3: 거제도 자생 남구절초, Group 4: 정선 자생 마키노국화). Group 별로 형태적 특성을 비교한 결과, 울릉국화(Group 2)의 꽃의 길이가 60.35mm로 가장 길었다. 또한, 구절초(Group 1)와 마키노국화(Group 4)는 기저부 잎 길이와 너비가 가장 길었으며, 엽록소 함량에는 큰 차이가 없었다. 상단부 잎의 경우 남구절초(Group 3)에서 길이(38.79mm), 너비(37.14mm), 엽록소 함량(37.13 SPAD)로 가장 컸다. 그러나 기저부 잎과 비교했을 때, 그 차이는 통계적으로 유의하지는 않았다. 마지막으로, 구절초(Group 1) 및 남구절초(Group 3)는 6배체, 울릉국화(Group 2)는 4배체, 마키노국화(Group 4)는 2배체였다. 본 연구는 국화 유전체 연구 및 기능성 육종을 위한 기초자료로 활용할 수 잇는 자원을 확보하기 위한 표준화 과정의 일환으로 수행되었다.

키워드
엽록소 함량
염색체 배수체
기능성 육종
산업 응용
표준화 과정
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 42
  • No :4
  • Pages :504-517
  • Received Date : 2023-06-19
  • Revised Date : 2023-08-07
  • Accepted Date : 2023-08-14
  • DOI :https://doi.org/10.7235/HORT.20240040
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