Variability of Chrysanthemum Cultivars Induced by Gamma Irradiation

Saika Anne; Jin Hee Lim

doi:10.7235/HORT.20210059

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2021 Vol.39, Issue 5 Preview Page Next Page

Research Article

Variability of Chrysanthemum Cultivars Induced by Gamma Irradiation

31 October 2021. pp. 660-672

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Abstract

This study aimed to determine the exposure dose of gamma radiation on stem cuttings of chrysanthemum cultivars. Explants of 11 cultivars (‘Eldora’, ‘Rania’, ‘Aileen’, ‘Olga pink’, ‘Sevda’, ‘Heimish’, ‘Rania’, ‘Attirance’, ‘White Plume’, ‘Coral King’, and ‘Leporem’) were subjected to different levels of gamma irradiation (0, 10, 30, 50, 70, 100, and 150 Gy). The results revealed that variation in vegetative growth parameters, viz., plant height, stem diameter, number of leaves, leaf length, leaf width, total number of flowers, flower diameter, number of petals, petal length and width, and number of buds occurred in the M1V1 generation compared to the control (0 Gy). The survival rates of the gamma-irradiated cultivars changed dramatically. The number of surviving plantlets based on 50% lethal dose (LD50) was found in 100 and 150 Gy treated plants 60 days after radiation. In addition, the highest percentage of plant survival was observed in the control, while the lowest percentage was observed in plants irradiated with 100 Gy and 150 Gy, along with delayed bud initiation and flowering. The number of days from planting to flowering was low in all of the control cultivars, even though the flowering percentage was high in the control compared to the treatments. The highest number of branches was observed in plants that were subjected to 100 and 150 Gy treatment, while 10, 30, and 50 Gy gamma irradiation revealed similar vegetative characteristics to all the control cultivars. Maximum leaf abnormality and leaf color variation were observed in the 70, 100, and 150 Gy treatments. Mutant varieties were produced from those treated with 10, 30, 50, 70, and 150 Gy, in which a higher number of mutants and mutation frequency were found in the 30 and 70 Gy treatments. The results showed that more flower color mutations occurred due to higher exposure to irradiation with minimum alteration in the vegetative growth in all cultivars. These results establish a standard to identify the gamma radiation dose that can induce mutations in chrysanthemum.

Keywords

flower color

growth parameter

lethal dose

mutation frequency

stem cutting

vegetative growth

References

Ambavane AR, Sawardekar SV, Sawantdesai SA, Gokhale NB (2015) Studies on mutagenic effectiveness and efficiency of gamma rays and its effect on quantitative traits in finger millet (Eleusine coracana L. Gaertn). J Radiat Res Appl Sci 8:120-12. doi:10.1016/j.jrras.2014.12.004 10.1016/j.jrras.2014.12.004

Anne S, Lim JH (2020) Mutation Breeding Using Gamma Irradiation in the Development of Ornamental Plants: A Review. Flower Res J 28:102-115. doi:10.11623/frj.2020.28.3.01 10.11623/frj.2020.28.3.01

Banerji BK, Datta SK (1992) Gamma rays induced flower shape mutation in Chrysanthemum cv 'Jaya'. J Nucl Agric Biol 21:73-9

Banerji BK, Datta SK (2002) Induction and analysis of gamma ray induced flower head shape mutation in 'Lalima' chrysanthemum (Chrysanthemum morifolium). Indian J Agric Sci 72:6-10

Beyaz R, Yildiz M (2017) The use of gamma irradiation in plant mutation breeding. Plant Eng 33-46. doi:10.5772/intechopen.69974 10.5772/intechopen.69974

Broertijes C, Roest S, Bokelmann GS (1976) Mutation breeding of Chrysanthemum morifolium Ram. using in vivo and in vitro adventitious bud techniques. Euphytica 25:11-19. doi:10.5772/intechopen.69974 10.5772/intechopen.69974

Broertjes C, van Harten AM (1988) Applied Mutation Breeding for Vegetatively Propagated Crops. Elsevier, Amsterdam

Brunner H (1995) Radiation induced mutations for plant selection. 4ppL Radiat 589-594. doi:10.1016/0969-8043(95)00096-8 10.1016/0969-8043(95)00096-8

Datta SK (1988) Chrysanthemum cultivars evolved by induced mutations at National Botanical Research Institute, Lucknow. Chrysanthemum 44:72-75

Datta SK (1990) Induction and analysis of somatic mutations in garden chrysanthemum. Adv Hortic Sci 1:241-254

Datta SK, Banerji BK, Gupta MN (1985) 'Tutika'-A new chrysanthemum cultivar evolved by gamma irradiation. J Nucl Agric Biol 14:160

Datta SK, Chakrabarty D, Mandal AKA (2001) Gamma ray‐induced genetic manipulations in flower colour and shape in Dendranthema grandiflorum and their management through tissue culture. Plant Breed 120:91-92. doi:10.1046/j.1439-0523.2001.00553.x 10.1046/j.1439-0523.2001.00553.x

Datta SK, Gupta MN (1981) Cytomorphological, palynological and biochemical studies on control and gamma induced mutants of Chrysanthemum. Sabrao J 13:136-148

Dilta BS, Sharma YD, Gupta YC, Bhalla R (2003) Effect of gamma rays on vegetative and flowering parameters of chrysanthemum. J Ornam Hort 6:328-334

Hoang TK, Wang Y, Hwang YJ, Lim JH (2020) Analysis of the morphological characteristics and karyomorphology of wild Chrysanthemum species in Korea. Hortic Environ Biotechnol 61:359-369. doi:10.1007/s13580-019-00222-9 10.1007/s13580-019-00222-9

Ibrahim R, Ahmad Z, Salleh S, Hassan AA, Ariffin S (2018) Ornamental Crops. Springer Vol.11 Ch.11

Kapoor M, Kumar P, Lal S (2014) Gamma radiation induced variations in corn marigold (Glebionis segetum) and their RAPD-based genetic relationship. Indian J Agric Sci 84:796-801

Killion DD, Constantin MJ (1971) Acute gamma irradiation of the wheat plant: effects of exposure, exposure rate, and developmental stage on survival, height, and grain yield. Radiation Botany 11:367-373. doi:10.1016/S0033-7560(71)90850-7 10.1016/S0033-7560(71)90850-7

Killion DD, Constantin MJ, Siemer EG (1971) Acute gamma irradiation of the soybean plant: effects of exposure, exposure rate and developmental stage on growth and yield. Radiation Botany 11:225-232. doi:10.1016/S0033-7560(71)90381-4 10.1016/S0033-7560(71)90381-4

Lee JW, Jo IH, Kim JU, Hong CE, Bang KH, Park YD (2019) Determination of mutagenic sensitivity to gamma rays in ginseng (Panax ginseng) dehiscent seeds, roots, and somatic embryos. Hortic Environ Biotechnol 60:721-731. doi:10.1007/s13580-019-00164-2 10.1007/s13580-019-00164-2

Matsumura A, Nomizu T, Furutani N, Hayashi K, Minamiyama Y, Hase Y (2010) Ray florets color and shape mutants induced by 12C5+ ion beam irradiation in chrysanthemum. Sci Hortic 123:558-661. doi:10.1016/j.scienta.2009.11.004 10.1016/j.scienta.2009.11.004

Momin KC, Gonge VS, Dalal SR, Bharad SG (2012) Radiation induced variability studies in chrysanthemum under net house. Asian Journal of Horticulture 7:524-527

Neary GJ, Evans HJ, Tonkinson SM (1957) Mitotic Delay Induced by Gamma Radiation in Broad Bean Root Meristems. Nature 179:917-918. doi:10.1038/179917a0 10.1038/179917a013430735

Nishiyama I, Ikushima T, Ichikawa S (1966) Radiobiological studies in plants XI: Further studies on somatic mutations induced by X-rays at the al locus of diploid oats. Radiation Botany 6:211-218. doi:10.1016/S0033-7560(66)80057-1 10.1016/S0033-7560(66)80057-1

Park YG, Jeong BR (2019) Night interruption light quality changes morphogenesis, flowering, and gene expression in Dendranthema grandiflorum. Hortic Environ Biotechnol 60:167-173. doi:10.1007/s13580-018-0114-z 10.1007/s13580-018-0114-z

Patil SD, Patil HE (2009) Improvement of major ornamental crops through mutation breeding. Int J Agric Sci 5:628-632

Patil UH, Karale AR, Katwate SM, Patil MS (2017) Mutation breeding in chrysanthemum (Dendrathema grandiflora T.). J Pharmacogn and Phytochem 6:230-232

Patil UH, Parhe SD, Pulate SC (2015) Induction of Somatic Mutation in Chrysanthemum Cultivar 'Local Golden'

Poole RT (1962) Effect of gamma rays on Chrysanthemum morifolium 'Bluechip'grown at various nutritional levels. J Florida State Hortic Soc 1962:443-447

Sadhukhan R, Swathi K, Sarmah D, Mandal T (2015) Effect of different doses of gamma rays on survivability and rooting ability in chrysanthemum (Chrysanthemum morifolim Ramat.). J Crop Weed 11:62-65

Sangeeta K, SR Dhiman, Gupta YC (2019) Advances in Breeding of Chrysanthemum: A Review. Int J Curr Microbiol App Sci 8:1631-1643. doi:10.20546/ijcmas.2019.808.193 10.20546/ijcmas.2019.808.193

Soliman TM, Lv S, Yang H, Hong B, Ma N, Zhao L (2014) Isolation of flower color and shape mutations by gamma radiation Chrysanthemum morifolium Ramat cv. Youka. Euphytica 199:317-324. doi:10.1007/s10681-014-1127-z 10.1007/s10681-014-1127-z

Su J, Jiang J, Zhang F, Liu Y, Ding L, Chen S, Chen F (2019) Current achievements and future prospects in the genetic breeding of chrysanthemum: a review. Hortic Res (Japan) 6:1-19. doi:10.1038/s41438-019-0193-8 10.1038/s41438-019-0193-831666962PMC6804895

van der Krol AR, Brunelle A, Tsuchimoto S, Chua NH (1993) Functional analysis of petunia floral homeotic MADS box gene pMADS1. Genes Dev 7:1214-1228. doi:10.1101/gad.7.7a.1214 10.1101/gad.7.7a.12148100547

Yamaguchi H, Shimizu A, Degi K, Morishita T (2008) Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum. Breed Sci 58:331-335. doi:10.1270/jsbbs.58.331 10.1270/jsbbs.58.331

Yamashita A (1964) Some aspects of radiosensitivity of crop plants under chronic exposure. In Gamma Field Symp 91-110

Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 39
No :5
Pages :660-672
Received Date : 2021-04-06
Revised Date : 2021-06-29
Accepted Date : 2021-07-13
DOI :https://doi.org/10.7235/HORT.20210059

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

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