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2016 Vol.34, Issue 4 Preview Page
Growth and Contents of Anthocyanins and Ascorbic Acid in Lettuce as Affected by Supplemental UV-A LED Irradiation with Different Light Quality and hotoperiod

상이한 광질 및 광주기 하에서 UV-A LED 부가 조사가 상추의 생장, 안토시아닌 및 아스코르빈산 함량에 미치는 영향

Yong Hyeon Kim1,2
,
Jae Su Lee3,4*
김 용현1,2
,
이 재수3,4*
Department of Bioindustrial Machinery Engineering, College of Agriculture & Life Sciences, Chonbuk National University
Institute for Agricultural Machinery & ICT Convergence, Chonbuk National University
Department of Bioindustrial Precision Machinery Engineering, Graduate School, Chonbuk National University
Farming Automation Division, Department of Agricultural Engineering, National Institute of Agricultural Sciences, RDA
전북대학교 농업생명과학대학 생물산업기계공학과
전북대학교 농업기계 ICT 융합연구소
전북대학교 대학원 생물산업정밀기계공학과
국립농업과학원 농업공학부 생산자동화기계과
*교신저자. *Corresponding Author. 
31 August 2016. pp. 596-606
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Abstract

The growth and contents of anthocyanins and ascorbic acid in lettuce(Lactuca sativa L., ‘Jeokchima’) as affected by supplemental UV-A LED irradiation under different light quality and photoperiod conditions were analyzed in this study. Five light qualities, namely B (blue LED), R (red LED), BUV (blue LED+UV-A LED), RUV (red LED+UV-A LED) and Control (white fluorescent lamps) with photoperiods of 12/12 hours (day/night), 16/8 hours, or 20/4 hours were provided to investigate the effects of light quality and photoperiod on the growth and accumulation of anthocyanins and ascorbic acid in lettuce leaves. As measured 28 days after transplanting, the number of leaves, leaf length, leaf width, leaf area, shoot fresh weight and dry weight of lettuce were significantly affected by light quality and photoperiod. The number of leaves, leaf length, leaf width, leaf area, shoot fresh weight and dry weight of lettuce grown under R treatment increased with increasing light period. By contrast, leaf development was inhibited, but chlorophyll content increased, under B treatment. Supplemental UV-A irradiation significantly decreased leaf length, leaf width, leaf area and shoot fresh weight. Anthocyanins in lettuce increased significantly with decreasing dark period under B treatment. A synergistic effect of supplemental UV-A LED irradiation on anthocyanins accumulation was found for lettuce leaves grown under R treatment but not B treatment. Ascorbic acid in lettuce was greatly affected by photoperiod. Ascorbic acid content at BUV and RUV treatments increased by 20-30% compared to without UV-A LED irradiation. From these results, it was concluded that growth and contents of anthocyanins and ascorbic acid in lettuce are significantly affected by supplemental UV-A LED irradiation. The results obtained in this study will be informative for efforts to improve the nutritional value of leafy vegetables grown in plant factories.

Keywords
antioxidation
artificial lighting source
phytochemicals
plant factory
ultraviolet

본 연구의 공시품종은 적치마 상추(Lactuca sativa L., cv. ‘Jeokchima’)로서 파종 후 본엽이 4매일 때 식물공장 내에 설치된 DFT 재배시스템에 정식하였다. 본 연구에서는 피크파장이 각각 450nm, 660nm, 365nm인 청색 LED, 적색 LED, UV-A LED를 이용하여 청색 LED (B), 적색 LED (R), 청색+UV-A LED (BUV), 적색+UV-A LED (RUV) 등 4개의 처리구를 설정하였고, 대조구로서 3파장 백색형광등을 사용하였다. 또한 광주기(명기/암기)에 따른 효과를 분석하고자 3수준(12/12h, 16/8h, 20/4h)의 광주기를 설정하였다. 식물공장 내 환경 조건은 기온(명기/암기) 22/18°C, 상대습도 70%, CO2 농도 800μmol· mol-1로 조절하였다. 한편 광합성유효광양자속과 UV-A LED의 조사강도는 각각 230 ± 11μmol· m-2· s-1, 80 ± 3mW· m-2이었다. 정식 후 28일째에 측정된 상추의 엽수, 엽장, 엽폭, 엽면적, 지상부 생체중 및 건물중은 본 연구에서 적용된 광질 및 광주기에 따라 유의차가 인정될 만큼 다르게 나타났다. 명기가 증가할수록 R 처리구에서의 엽수, 엽장, 엽폭, 엽면적, 지상부 생체중 및 건물중은 증가하였다. 한편 B 처리구에서는 잎의 신장이 억제되었으나, 엽록소함량은 증가하였다. UV-A의 부가 조사로 인하여 엽장, 엽폭, 엽면적 및 지상부 생체중이 감소하였다. 그러나 상추 잎의 안토시아닌 함량은 청색광 하에서 많이 증가하였으며, 명기가 짧아질수록 높게 축적되었다. 한편 UV-A의 부가 조사에 따른 안토시아닌 함량의 증가 효과는 R 처리구에서 높게 나타났다. 상추 잎의 아스코르빈산 함량은 광주기의 영향을 크게 받았다. 청색 또는 적색 LED에서 UV-A LED의 부가 조사에 따라 아스코르빈산 함량이 20-30% 증가하였다. 결론적으로 상추의 생장, 안토시아닌 및 아스코르빈산 함량에 미치는 UV-A LED의 조사 효과가 분명하게 나타났다. 향후 폐쇄형 시스템에서 엽채류의 영양성을 향상시키는 데 필요한 UV-A LED의 운용 조건을 제시하고자 할 때 본 연구 결과가 기초 자료로서 유용하게 활용될 것이다.

키워드
항산화
인공광원
파이토케미컬
식물공장
자외선
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 34
  • No :4
  • Pages :596-606
  • Received Date : 2016-01-27
  • Revised Date : 2016-04-28
  • Accepted Date : 2016-06-22
  • DOI :https://doi.org/10.12972/kjhst.20160061
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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