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2014 Vol.32, Issue 5 Preview Page
Construction of a Network Model to Reveal Genes Related to Salt Tolerance in Chinese Cabbage

배추 염 저항성 관련 유전자의 네트워크 모델 구축

Gi-Ho Lee1
,
Jae-Gyeong Yu1
,
Ji-Hyun Park1
,
Young-Doo Park1*
이 기호1
,
유 재경1
,
박 지현1
,
박 영두1*
1Department of Horticultural Biotechnology, Kyunghee University
1경희대학교 생명과학대학 원예생명공학과
*교신저자. *Corresponding Author. 
31 October 2014. pp. 684-693
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Abstract

Abiotic stress conditions such as cold, drought, and salinity trigger physiological and morphological changes and yield loss in plants. Hence, plants adapt to adverse environments by developing tolerance through complex regulation of genes related to various metabolic processes. This study was conducted to construct a coexpression network for multidirectional analysis of salt-stress response genes in Brassica rapa (Chinese cabbage). To construct the coexpression network, we collected KBGP-24K microarray data from the B. rapa EST and microarray database (BrEMD) and performed time-based expression analyses of B. rapa plants. The constructed coexpression network model showed 1,853 nodes, 5,740 edges, and 142 connected components (correlation coefficient > 0.85). On the basis of the significantly expressed genes in the network, we concluded that the development of salt tolerance is closely related to the activation of Na+ transport by reactive oxygen species signaling and the accumulation of proline in Chinese cabbage.

Keywords
abiotic stress
microarray
jasmonic acid
reactive oxygen species

저온, 건조, 염과 같은 비생물적 스트레스는 식물의 생리적 형태적 변화와 수확량 감소를 초래한다. 이러한 이유로 식물체는 불리한 환경을 극복하기 위해 다양한 대사과정에 관련된 유전자들간의 복잡한 상호 관계를 조절함으로써 저항성을 획득한다. 본 연구는 배추에서 염 스트레스에 반응하는 유전자를 다각적으로 분석하기 위해 상호발현 네트워크를 구축하였다. 네트워크를 구축하기 위하여 배추를 염 스트레스 조건 하에서 시간 경과에 따라 KBGP-24K 마이크로어레이 분석을 실시한 [BrEMD (Brassica rapa EST and Microarray Database)] 실험 결과를 수집하여 분석하였다. 구축된 네트워크 모델은 1,853개 node, 5,740개 edge, 및 142개 connected component(상관계수 > 0.85)로 구성되었다. 구축된 네트워크 분석 결과, ROS 신호 전달을 통한 Na+ 수송 활성화와 proline 축적이 배추의 염 저항성 획득과 밀접한 연관이 있는 것으로 판단하였다.

키워드
비생물적 스트레스
마이크로어레이
자스몬산
활성산소
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 32
  • No :5
  • Pages :684-693
  • Received Date : 2014-02-27
  • Revised Date : 2014-04-12
  • Accepted Date : 2014-04-29
  • DOI :https://doi.org/10.7235/hort.2014.14034
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