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2014 Vol.32, Issue 5 Preview Page
High-frequency Plant Regeneration from Cultured Flower Bud Receptacles of Allium hookeri L.
Ja Choon Koo1*
1Division of Science Education and Institute of Fusion Science, Chonbuk National University
*교신저자. *Corresponding Author. 
31 October 2014. pp. 694-701
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Abstract

Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots (93.33 ± 4.63%), roots (76.67 ± 7.85%), and calli (80.00 ± 7.43%) when cultured on Gamborg B5 (B5) medium containing 10 μM 6-benzylaminopurine (BA) + 1 μM naphthalene acetic acid (NAA), 0.5 μM BA + 5 μM NAA, and 1 μM BA + 10 μM NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to 10 μM. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.

Keywords
1-naphthalene acetic acid (NAA)
6-benzyladenine (BA)
Allium hookeri
micropropagation
receptacle

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 32
  • No :5
  • Pages :694-701
  • Received Date : 2014-02-10
  • Revised Date : 2014-05-19
  • Accepted Date : 2014-06-08
  • DOI :https://doi.org/10.7235/hort.2014.14023
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