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2015 Vol.33, Issue 1 Preview Page
Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

멜론 덩굴쪼김병에 대한 효율적인 저항성 검정법 개발

Won Jeong Lee1,2
,
Ji Hyun Lee1
,
Kyoung Soo Jang1
,
Yong Ho Choi1
,
Heung Tae Kim2
,
Gyung Ja Choi1*
이 원정1,2
,
이 지현1
,
장 경수1
,
최 용호1
,
김 흥태2
,
최 경자1*
1Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
2Department of Plant Medicine, Chungbuk National University
1한국화학연구원 바이오화학연구센터
2충북대학교 식물의학과
*교신저자. *Corresponding Author. 
28 February 2015. pp. 70-82
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Abstract

This study was conducted to establish an efficient screening system to identify melon resistant to Fusarium oxysporum f. sp. melonis. F. oyxsporum f. sp. melonis GR was isolated from infected melon plants collected at Goryeong and identified as F. oxysporum f. sp. melonis based on morphological characteristics, molecular analyses, and host-specificity tests on cucurbits including melon, oriental melon, cucumber, and watermelon. In addition, the GR isolate was determined as race 1 based on resistance responses of melon differentials to the fungus. To select optimized medium for mass production of inoculum of F. oxysporum f. sp. melonis GR, six media were tested. The fungus produced the most spores (microconidia) in V8-juice broth. Resistance degrees to the GR isolate of 22 commercial melon cultivars and 6 rootstocks for melon plants were investigated. All tested rootstocks showed no symptoms of Fusarium wilt. Among the tested melon cultivars, only three cultivars were susceptible and the other cultivars displayed moderate to high resistance to the GR isolate. For further study, six melon cultivars (Redqueen, Summercool, Superseji, Asiapapaya, Eolukpapaya, and Asiahwanggeum) showing different degrees of resistance to the fungus were selected. The development of Fusarium wilt on the cultivars was tested according to several conditions such as plant growth stage, root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease. On the basis of the test results, we suggest that an efficient screening method for melon plants resistant to F. oxysporum f. sp. melonis is to remove soil from roots of seven-day-old melon seedlings, to dip the seedlings without cutting in spore suspension of 3 × 105 conidia/mL for 30 min, to transplant the inoculated seedlings to plastic pots with horticulture nursery media, and then to cultivate the plants in a growth room at 25 to 28°C for about 3 weeks with 12-hour light per day.

Keywords
bioassay
breeding
cucurbit
disease resistance
Fusarium wilt

본 연구는 덩굴쪼김병균(Fusarium oxysporum f. sp. melonis)에 대한 저항성 멜론의 효율적인 검정법을 확립하기 위하여 수행하였다. 고령에서 채집한 덩굴쪼김병이 발생한 멜론으로부터 GR 균주를 분리하였으며, 형태학적 및 분자생물학적 동정 방법에 의해 그리고 오이, 멜론, 참외, 수박의 박과 작물에 대한 기주 특이성 조사를 통하여 GR 균주는 F. oxysporum f. sp. melonis로 동정되었다. 그리고 4종 덩굴쪼김병 race 판별품종들의 저항성 반응에 따라 GR 균주는 race 1임을 알 수 있었다. GR 균주의 접종원(소형분생포자) 대량생산을 위해서는 실험한 6종 액체배지 중 V8-juice broth에서 가장 많은 포자가 형성되었다. 시판 중인 22개의 멜론 품종과 6개 멜론 재배용 대목 품종의 GR 균주에 대한 저항성의 정도를 실험하였다. 실험한 멜론 품종 중 3개 품종을 제외한 모든 품종은 다양한 정도의 저항성을 보였다. 그리고 실험한 대목 품종들 모두에서는 덩굴쪼김병이 전혀 발생하지 않았다. 실험한 멜론 품종 중 GR 균주에 대한 저항성 반응에 차이를 보이는 6개 품종(‘레드퀸’, ‘썸머쿨’, ‘슈퍼세지’, ‘아시아파파야’, ‘얼룩파파야’, ‘아시아황금’)을 선발하여 멜론 생육시기, 뿌리 상처, 침지 시간, 접종원 농도 및 재배 온도 등의 발병 조건에 따른 덩굴쪼김병 발생을 조사하였다. 이들 실험의 결과로부터 멜론 품종들의 덩굴쪼김병에 대한 저항성을 검정하기 위해서는 멜론 종자를 파종하고 온실(25 ± 5°C)에서 7일 동안 재배한 유묘(떡잎 시기)를 뽑아 흙을 제거하고 뿌리 자르기와 같은 상처를 내지 않고 멜론 유묘의 뿌리를 3 × 105conidia/mL 농도의 F. oxysporum f. sp. melonis 포자현탁액에 30분 정도 침지하여 접종하고, 이를 새로운 토양에 이식하고 25-28°C에서 약 3주일 동안 재배하는 것이 가장 효율적인 방법임을 알 수 있었다.

키워드
병리검정
육종
박과 작물
내병성
Fusarium 시들음병
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 33
  • No :1
  • Pages :70-82
  • Received Date : 2014-06-08
  • Revised Date : 2014-07-25
  • Accepted Date : 2014-09-17
  • DOI :https://doi.org/10.7235/hort.2015.14101
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