ROS-mediated Lignin Biosynthesis is Associated with Cell Wall Expansion in Asian Pear (Pyrus pyrifolia Nakai) During Fruit Development and Ripening

Bok-Rye Lee; Yun-Jun Baek; Seung Gon Wi; Ung Yang; Sang-Hyun Lee

doi:10.7235/HORT.20220032

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2022 Vol.40, Issue 4 Next Page

Research Article

ROS-mediated Lignin Biosynthesis is Associated with Cell Wall Expansion in Asian Pear (Pyrus pyrifolia Nakai) During Fruit Development and Ripening

31 August 2022. pp. 349-360

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Abstract

Fruit growth and development is regulated by cell expansion, which is a culmination of balanced cell stiffening and loosening. Reactive oxygen species (ROS) are crucial factors regulating cell expansion. In the present study, we investigated how cell wall expansion is regulated by ROS homeostasis in two Asian pear (Pyrus pyrifolia Nakai) cultivars with different maturity periods. ROS production, lignin contents, peroxidase activity, and gene expression levels were measured during pear fruit development and ripening. An increase in fruit fresh weight was observed 72 and 87 days after full bloom (DAFB) in early-maturing cultivar ‘Wonhwang’ and late-maturing cultivar ‘Niitaka’, respectively; the former exhibited higher weights than the latter until 132 DAFB. In both cultivars, ROS (O₂^•– and H₂O₂) content gradually decreased up to 132 DAFB; higher levels were detected in ‘Niitaka’ than in ‘Wonhwang’, which was consistent with the superoxide dismutase (SOD) activity. H₂O₂-induced lignin content decreased after maintaining a high level up to 87 DAFB in ‘Niitaka’, whereas it continued to decrease during fruit development and ripening after 57 DAFB in ‘Wonhwang’. In ‘Niitaka’, activities of lignin biosynthesis-related enzymes, guaiacol peroxidase (GPOX) and coniferyl alcohol peroxidase (CPOX), rapidly decreased from 102 to 132 DAFB, but remained higher than those in ‘Wonhwang’; this result was consistent with the expression patterns of peroxidase genes (PpPOX1 and PpPOX4). The expression level of the cell wall loosening-related gene, expansin 1 (PpEXP1), was elevated only in the middle stage of fruit development and ripening, while the expression level of xyloglucan endotransglucosylases 1 (PpXET1) rapidly increased after 57 and 87 DAFB in ‘Wonhwang’ and ‘Niitaka’, respectively. These results indicate that ROS-mediated lignin synthesis through peroxidase is associated with cell wall expansion in Pyrus pyrifolia.

Keywords

cell wall remodeling-related gene

cell wall rigidity

early-maturing cultivar

hydrogen peroxide

late-maturing cultivar

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Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 40
No :4
Pages :349-360
Received Date : 2022-02-03
Revised Date : 2022-05-26
Accepted Date : 2022-06-22
DOI :https://doi.org/10.7235/HORT.20220032

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

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