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2025 Vol.43, Issue 5 Preview Page

Research Article

Differential Physiological and Growth Responses of Lettuce Seedlings to Extreme Thermal Stress Analyzed through Chlorophyll Fluorescence
Yu Kyeong Shin123
,
Jun Gu Lee345*
1Department of Horticultural Science, College of Agricultural & Life Science, Kyungpook National University, Daegu 41566, Korea
2Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Korea
3Department of Horticulture, College of Agriculture & Life Sciences, Jeonbuk National University, Jeonju 54896, Korea
4Core Research Institute of Intelligent Robots, Jeonbuk National University, Jeonju 54896, Korea
5Institute of Agricultural Science & Technology, Jeonbuk National University, Jeonju 54896, Korea
*Corresponding Author
31 October 2025. pp. 604-622
PDF XML Citation
Abstract

This study sought to monitor changes in growth parameters, chlorophyll fluorescence (CF) levels, and proline and chlorophyll contents in lettuce seedlings grown under different temperature conditions. Ten-day-old lettuce seedlings grown under a fluorescent lamp in a closed plant production system having a constant temperature (24/18°C), humidity of 60 ± 3%, and a light period of 14/10 h (day/night) were kept at 24/18°C for three days in a closed chamber under white warm LED lighting at 220 ± 10 µmol∙m-2∙s-1. The seedlings were then grown under different temperature conditions [low: 8/4°C (day/night), control: 24/20°C, and high: 40/36°C] in a closed and controlled plant production system for eight days. The CF parameters, proline amount, and chlorophyll content were measured at 0, 2, 4, 6, and 8 d after the initiation of the treatment, while the growth parameters (fresh weight, dry weight, leaf length, and hypocotyl length) were measured at the end of the experiment. The quantum yield of nonregulated energy dissipation in PSII [Y(NO)] increased on the second day and then decreased at low temperatures. Non-photochemical quenching (NPQ) and the fluorescence decrease ratio (Rfd) significantly decreased under both high- and low-temperature stress compared to those in the control. Growth parameters were retarded at low temperatures, whereas leaves became dry at high temperatures. The chlorophyll content decreased significantly, whereas the proline content increased only at low temperatures. These results indicate that Y(NO), NPQ, and Rfd can be used to detect temperature stress in lettuce seedlings.

Keywords
abiotic stress
leafy vegetables
photosynthesis
phytochemical
rapid detection
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :5
  • Pages :604-622
  • Received Date : 2025-03-22
  • Revised Date : 2025-04-21
  • Accepted Date : 2025-05-20
  • DOI :https://doi.org/10.7235/HORT.20250055
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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