All Issue

2015 Vol.33, Issue 2 Preview Page
Effect of Developmental Stages on Glucosinolate Contents in Kale (Brassica oleracea var. acephala)

생장단계에 따른 케일 내 글루코시놀레이트 함량

Heon-Hak Lee1
,
Si-Chang Yang1
,
Min-Ki Lee1
,
Dong-Ki Ryu2
,
Suhyoung Park3
,
Sun-Ok Chung2
,
Sang Un Park4
,
Yong-Pyo Lim5
,
Sun-Ju Kim1*
이 헌학1
,
양 시창1
,
이 민기1
,
류 동기2
,
박 수형3
,
정 선옥2
,
박 상언4
,
임 용표5
,
김 선주1*
1Department of Bio-Environmental Chemistry, Chungnam National University,
2Department of Biosystems Machinery Engineering, Chungnam National University
3Department of Horticultural Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA)
4Department of Crop Science, Chungnam National University
5Department of Horticultural Science, Chungnam National University
1충남대학교 생물환경화학과
2충남대학교 바이오시스템기계공학과
3농촌진흥청 국립원예특작과학원
4충남대학교 식물자원학과
5충남대학교 원예학과
*교신저자. *Corresponding Author. 
30 April 2015. pp. 177-185
PDF XML Citation
Abstract

The aim of this study was to investigate the amounts of glucosinolates (GSL) in kale at various development stages. Kale varieties ‘Manchoo Collard’ and ‘TBC’ were cultivated from 20 February 2012 to 3 July 2013 in the greenhouse at Chungnam National University. During the cultivation periods, samples were harvested at 35, 63, 91, 105, 119, and 133 days after sowing (DAS) and the amount of GSL quantified by HPLC. Ten types of GSL (progoitrin, sinigrin, glucoalyssin, gluconapin, glucoiberverin, 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin) were observed in ‘TBC’, whereas nine types of GSL (the same as above, except glucoiberverin) were identified in ‘Manchoo Collard’. The amount of total GSL in ‘Manchoo Collard’ was comparatively higher at 133 DAS (mean 8.64 μmol･g-1) and lower at 35 DAS (1.16 μmol･g-1 dry weight, DW) of cultivation. In the case of ‘TBC’, the amount of GSL was higher at 91 DAS (mean 13.41 μmol･g-1) and lower at 35 DAS (0.31 μmol･g-1 dry weight, DW). Sinigrin was the most abundant GSL (57% of total GSL) in ‘Manchoo Collard’ at 133 DAS and was also highest (44%) in ‘TBC’ at 91 DAS. Together, progoitrin, sinigrin, glucobrassicin, and gluconasturtiin, the precursor of crambene, allylisothiocyanate, indol-3-cabinol, and phenethylisothiocyanate accounted for 94 and 78% of GSL in ‘Manchoo Collard’ and ‘TBC’, respectively. Our results demonstrate that the amounts of GSL, which have potential anti-carcinogenic activity, change during development in kale.

Keywords
Brassica vegetable
HPLC analysis
‘Manchoo Collard’
sinigrin
‘TBC’

케일 내 유용성분인 GSL 함량은 작물의 재배방법과 기후조건, 영양상태, 유전적 특성 등 재배 환경에 따라 영향을 받을 수 있으므로 본 연구에서는 생장단계에 따른 케일(Brassica oleracea var. acephala) 내 GSL 함량을 조사하였다. 케일 품종은 ‘Manchoo Collard’, ‘TBC’ 두 품종으로 재배기간은 2012년 2월 20일부터 동년 7월 3일까지 133일이었으며 수확시기는 파종 후 35, 63, 91, 105, 119, 133일(days after sowing, DAS)이었다. ‘TBC’에서는 총 10종의 GSL (progoitrin, sinigrin, glucoalyssin, gluconapin, glucoiberverin, 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, neoglucobrassicin)가 분리 및 동정되었으나 ‘Manchoo Collard’에서는 glucoiberverin를 제외한 9종의 GSL가 분리 및 동정되었다. ‘Manchoo Collard’의 GSL 함량은 133DAS에서(평균 6.12μmol･g-1 DW)가장 높았고, 35DAS (1.16μmol･g-1 DW)에서 가장 낮았다. ‘TBC’의 GSL 함량은 91DAS(평균 13.41μmol･g-1 DW)에서 가장 높았고, 35DAS (0.31μmol･g-1 DW)에서 가장 낮았다. 총 GSL 함량 중 sinigrin이 ‘Manchoo Collard’(133DAS)에서 57%, ‘TBC’(91DAS)에서 44%로 가장 높았다. 항암효과가 뛰어난 crambene, allyl isothiocynate, indole-3-cabinol, phenethyl isothiocyanate의 전구체인 progoitrin, sinigrin, glucobrassicin, gluconasturtiin은 ‘Manchoo Collard’와 ‘TBC’의 총 GSL 함량 중 각각 94, 78%로 나타나 두 품종 모두 항암 효과를 가질 것으로 기대된다.

키워드
배추과 채소
HPLC 분석
‘만추콜라드’
sinigrin
‘케일티비시’
References
1
Agerbirk, N. and C.E. Olsen. 2012. Glucosinolate structures in evolution. Phytochemistry 77:16-45.
2
Cartea, M.E., P. Velasco, S. Obregón, G. Padilla, and A. de Haro. 2008. Seasonal variation in glucosinolate content in Brassica oleracea crops grown in northwestern Spain. Phytochemistry 69:403-410.
3
Choi, Y.H., K.Y. Park, S.M. Lee, M.A. Yoo, and W.H. Lee. 1995. Inhibitory effect of the fresh juice of kale on the genotoxicity of aflatoxin B1. Korean J. Genetic. 17:183-190.
4
Clarke, D.B. 2010. Glucosinolates, structures and analysis in food. Anal. Methods 2:310-325.
5
Fahey, J.W., A.T. Zalcmann, and P. Talalay. 2001. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5-51.
6
Goncalves, Á.L.M., M. Lemos, R. Niero, S.F. de Andrade, and E.L. Maistro. 2012. Evaluation of the genotoxic and antigenotoxic potential of Brassica oleracea L. var. acephala D.C. in different cells of mice. J. Ethnopharmacol. 143:740-745.
7
Hagen, S.F., G.I.A Borge, K.A. Solhaug, and G.B. Bengtsson. 2009. Effect of cold storage and harvest date on bioactive compounds in curly kale (Brassica oleracea L. var. acephala). Postharvest Biol. Technol. 51:36-42.
8
Halkier, B.A. and L. Du. 1997. The biosynthesis of glucosinolates. Trends Plant Sci. 2:425-431.
9
Halvorsen, B.L., K. Holte, M.C.W. Myhrstad, I. Barikmo, E. Hvattum, S.F. Remberg, A.B. Wold, K. Haffner, H. Baugerød, L.F. Andersen, Ø. Moskaug, D.R. Jr. Jacobs DR. and R. Blomhoff. 2002. A systematic screening of total antioxidants in dietary plants. J. Nutr. 132:461-471.
10
Higdon, J.V., B. Delage, D.E. Williams, and R.H. Dashwood. 2007. Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol. Res. 55:224-236.
11
Hwang, E.S., E.Y. Hong and G.H Kim. 2012. Determination of bioactive compounds and anti-biocancer effect from extracts of Korean cabbage and cabbage. Korean J. Food Nutr. 25:259-265.
12
International Standards Organization. 1992. Rapeseed: Determination of glucosinolates. Part 1: Method using High performance liquid chromatography, p. 1-9. In: ISO 9167-1. Geneva, Switzerland.
13
Keck, A.S. and J.W. Finley. 2004. Cruciferous vegetables: cancer protective mechanisms of glucosinolate hydrolysis products and selenium. Integr. Cancer Ther. 3:5-12.
14
Kim, S.J., C. Kawaharada, S. Jin, M. Hashimoto, G. Ishii, and H. Yamauchi. 2007. Structural elucidation of 4-(cystein-S-yl)butyl glucosinolate from the leaves of Eruca sativa. Biosci. Biotechnol. Biochem. 71:114-121.
15
Kim, Y.S. and J.A. Milner. 2005. Targets for indole-3-carbinol in cancer prevention. J. Nutr. Biochem. 16:65-73.
16
Kushad, M.M., A.F. Brown, A.C. Kurilich, J.A. Juvik, B.P. Klein, M.A. Wallig, and E.H. Jeffery. 1999. Variation of glucosinolates in vegetable crops of Brassica oleracea. J. Agric. Food Chem. 47:1541-1548.
17
Kushad, M.M., R. Cloyd, and M. Babadoost. 2004. Distribution of glucosinolates in ornamental cabbage and kale cultivars. Sci. Hortic. 101:215-221.
18
Lee, S.M., S.H. Rhee, and K.Y. Park. 1997. Antimutagenic effect of various cruciferous vegetables in salmonella assaying system. J. Food Hyg. Saf. 12:321-327.
19
Lim, H.S. 2002. The study for contents of sinigrin in dolsan leaf mustard kimchi during fementation periods. Korean J. Life Sci. 12:523-527.
20
Magrath, R., F. Bano, M. Morgner, I. Parkin, A. Sharpe, C. Lister, C. Dean, J. Turner, D. Lydiate, and R. Mithen. 1994. Genetics of aliphatic glucosinolates. I. Side chain elongation in Brassica napus and Arabidopsis thaliana. Heredity 72: 290-299.
21
Podsędek, A. 2007. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Sci. Technol. 40:1-11.
22
Sun, B., N. Liu, Y. Zhao, H. Yan, and Q. Wang. 2011. Variation of glucosinolates in three edible parts of Chinese kale (Brassica alboglabra Bailey) varieties. Food Chem. 124:941-947.
23
Zhang, Y. and P. Talalay. 1994. Anticarcinogenic activities of organic isothiocyanates: Chemistry and mechanisms. Cancer Res. 54:1976-1981.
24
Zhang, Z., J.A. Ober, and D.J. Kliebenstein. 2006. The gene controlling the quantitative trait locus epithiospecifier modifier1 alters glucosinolate hydrolysis and insect resistance in Arabidopsis. Plant Cell 18:1524-1536.
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 33
  • No :2
  • Pages :177-185
  • Received Date : 2014-01-28
  • Revised Date : 2014-08-26
  • Accepted Date : 2014-11-02
  • DOI :https://doi.org/10.7235/hort.2015.14017
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
Online Submission
  • scopus
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close