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Research Article

Genetic Relationship Analysis by using FT-IR Spectrum of Genomic DNA from Peach Cultivars Related to ‘Yumyeong’

Genomic DNA의 FT-IR 스펙트럼을 이용한 복숭아 ‘유명’ 관련 품종의 유연관계 분석

Seung-Yeob Song1
,
Ju-Hyun Lee1
,
Kidong Hwang1
,
Namhee Jeong1
,
In Myung Choi1
,
Jung Hyun Kwon1†
송 승엽1
,
이 주현1
,
황 기동1
,
정 남희1
,
최 인명1
,
권 정현1†
1Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea
1농촌진흥청 국립원예특작과학원 과수과
*kwon1101@korea.kr
28 February 2021. pp. 122-131
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Abstract

The genetic relationships of offspring cultivars from ‘Yumeyong’ peach were evaluated by using Fourier transform infrared (FT-IR) spectroscopy. Four offspring cultivars were bred by controlled hybridization between ‘Yumyeong’ and ‘Chiyomaru’, 2 from open pollination, and 1 from bud sport mutation. The FT-IR spectrum of genomic DNA were analyzed by using principal component analysis (PCA), partial least square discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA). FT-IR spectra showed that typical spectral differences were existed in the frequency regions of guanine ring (C6 = C6), thymine ring (C4 = O4), cytosine, N-H stretching (amide I), C = O stretching vibrations (amide II), and PO2- ionized asymmetric and symmetric stretching, respectively. Among the offspring cultivars between ‘Yumyeong’ and ‘Chiyomaru’, ‘Mihong’ and ‘Misshong’ showed higher genetic similarity to ‘Yumyeong’ than ‘Chiyomaru’. On the other hand, ‘Yumi’ and ‘Soomee’ showed higher genetic similarity to ‘Chiyomaru’ than ‘Yumyeong’. ‘Wolmi’, a mutant cultivar from ‘Yumyeong’, showed close relationship with ‘Yumyeong’, however, the genetic similarity was lower compared to other crossbreeding cultivars, ‘Misshong’, and ‘Mihong’.

Keywords
discrimination system
hierarchical clustering analysis
multivariate analysis
partial least square discriminant analysis (PLS-DA)
principal component analysis (PCA)

본 연구는 FT-IR 스펙트럼 데이터를 기반으로 다변량통계 기법을 이용하여 ‘유명’ 복숭아 자손 품종의 유전적 관계를 분석하였다. ‘유명’과 ‘치요마루’의 교배를 통해 육성된 4품종, ‘유명’ 품종의 자연교잡실생 2품종 그리고 아조변이 1품종을 사용하였다. Genomic DNA의 FT-IR 스펙트럼 데이터로부터 principal component analysis(PCA), partial least square discriminant analysis (PLS-DA) 그리고 hierarchical clustering analysis(HCA) 분석을 실시하였다. IR 스펙트럼은 구아닌 고리(C6 = C6), 티민 고리(C4 = O4), 사이토신, N-H 스트레칭(amide I), C = O 스트레칭 진동(amide II) 및 PO2- 이온화 대칭과 비대칭 스트레칭의 주파수 영역에서 전형적인 스펙트럼 차이가 존재함을 나타낸다. ‘유명’과 ‘치요마루’의 교배 품종 중 ‘미홍’과 ‘미스홍’은 ‘치요마루’보다 ‘유명’에 유전적 유사성이 더 높았다. 반면 ‘유미’와 ‘수미’는 ‘유명’보다 ‘치요마루’와 유전적 유사성이 더 높았다. ‘유명’의 변이 품종 ‘월미’는 ‘유명’과 밀접한 관계를 보였지만, 다른 교배 품종인 ‘미홍’과 ‘미스홍’에 비해 유전적 유사성이 낮았다.

키워드
식별시스템
위계적군집분석
다변량통계분석
부분최소제곱회귀
주성분분석
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :1
  • Pages :122-131
  • Received Date : 2020-07-01
  • Revised Date : 2020-09-18
  • Accepted Date : 2020-11-06
  • DOI :https://doi.org/10.7235/HORT.20210011
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