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Effects of Shading Levels on the Growth, Flowering, Photosynthetic Characteristics, and Spectral Reflectance of Coreanomecon hylomeconoides Nakai
Yong Seung Roh1
,
Yong Kweon Yoo2*
1Gangjin-gun Agricultural Technology & Extension Center, Gangjin 59223, Korea
2School of Horticulture and Forestry, College of Life and Medical Science, Mokpo National University, Muan 58554, Korea
*Corresponding Author
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Abstract

This study investigated the effects of different shading levels (0, 45, 70, and 90% shade) on the growth, flowering, photosynthetic characteristics, and spectral reflectance to determine the optimal light environment in which to cultivate Coreanomecon hylomeconoides. Plant height, leaf length, and leaf width increased when increasing the shading intensity, whereas the number of leaves, shoot fresh weight, and shoot dry weight were greatest under the 70% shade treatment. Plants grown under the 0% shade treatment exhibited poor growth, leaf chlorosis, and significantly lower chlorophyll content levels than shaded plants. Flower and flower stalk numbers were significantly higher under the 45% and 70% shade treatments than under the 90% shade treatment. The maximum net photosynthetic rate (Amax) and light saturation point (LSP) were significantly lower in the non-shaded treatment compared to all of the shaded treatments. Under the 0% shade treatment, chlorophyll fluorescence parameters associated with photosystem II efficiency, in this case Fv/Fo, Fv/Fm, ETo/TRo, ETo/ABS, ETo/RC, and PIABS, decreased, indicating excessive irradiance that caused photoinhibition. A spectral reflectance analysis also showed increased MCARI and NPCI values and decreased SRPI and PRI values under the 0% shade treatment, suggesting enhanced physiological stress and a pigment imbalance. Therefore, 45% and 70% shade treatments are recommended as the optimal light environment to cultivate and produce ornamental C. hylomeconoides, with a 70% shade treatment providing the most favorable overall performance.

Keywords
chlorophyll fluorescence
endemic plant
photoinhibition
photosynthetic rate
photosystem II
References
1

Baker NR (2008) Chlorophyll fluorescence: A probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89-113. https://doi.org/10.1146/annurev.arplant.59.032607.092759

10.1146/annurev.arplant.59.032607.092759
2

Barták M, Hájek J, Amarillo AC, Hazdrová J, Carreras H (2016) Changes in spectral reflectance of selected Antarctic and South American lichens caused by dehydration and artificially-induced absence of secondary compounds. Czech Polar Rep 6:221-230. https://doi.org/10.5817/CPR2016-2-20

10.5817/CPR2016-2-20
3

Beak JJ (2021) Ecophysiological implications of morphophysiological dormancy (MPD) with different seed maturation time of Coreanomecon hylomeconoides Nakai, an endemic perennial herb in the Korean peninsula. MS thesis, Andong National University

4

Boardman NK (1977) Comparative photosynthesis of sun and shade plants. Ann Rev Plant Physiol 28:355-377. https://doi.org/10.1146/annurev.pp.28.060177.002035

10.1146/annurev.pp.28.060177.002035
5

Boureima S, Oukarroum A, Diouf M, Cisse N, Van Dammr P (2012) Screening for drought tolerance in mutant germplasm of sesame (Sesamum indicum) probing by chlorophyll a fluorescence. Environ Exp Bot 81:37-43. https://doi.org/10.1016/j.envexpbot.2012.02.015

10.1016/j.envexpbot.2012.02.015
6

Cahill AE, Aiello-Lammens ME, Fisher-Reid MC, Hua X, Karanewsky CJ, Ryu HY, Sbeglia GC, Spagnolo F, Waldron JB, et al. (2013) How does climate change cause extinction? Proc R Soc B 280:20121890. http://doi.org/10.1098/rspb.2012.1890

10.1098/rspb.2012.189023075836PMC3574421
7

Casal JJ (2012) Shade avoidance. The arabidopsis book 2012:e0157. https://doi.org/10.1199/tab.0157

10.1199/tab.015722582029PMC3350169
8

Casal JJ (2013) Photoreceptor signaling networks in plant responses to shade. Annu Rev Plant Biol 64:403–427. https://doi.org/10.1146/annurev-arplant-050312-120221

10.1146/annurev-arplant-050312-120221
9

Choi HG, Jeong HJ (2020) Comparison of chlorophyll fluorescence and photosynthesis of two strawberry cultivars in response to relative humidity. Hortic Sci Technol 38:66-77. https://doi.org/10.7235/HORT.20200007

10.7235/HORT.20200007
10

Chung GY, Jang HD, Chang KS, Choi HJ, Kim YS, Kim HJ, Son DC (2023) A checklist of endemic plants on the Korean peninsula II. Korean J Plant Taxo 53:79-101. https://doi.org/10.11110/kjpt.2023.53.2.79

10.11110/kjpt.2023.53.2.79
11

Coelho N, Gonçalves S, Romano A (2020) Endemic plant species conservation: Biotechnological approaches. Plants 9:345. https://doi.org/10.3390/plants9030345

10.3390/plants903034532182892PMC7154900
12

Demotes-Mainard S, Péron T, Corot A, Bertheloot J, Gourrierec JL, Pelleschi-Travier S, Crespel L, Morel P, Huché-Thélier L, et al. (2016) Plant responses to red and far-red lights, applications in horticulture. Environ Exp Bot 121:4-21. http://doi.org/10.1016/j.envexpbot.2015.05.010

10.1016/j.envexpbot.2015.05.010
13

Dey P, Pattanaik D, Dash D, Singhal RK, Gaikwad DJ, Baig MJ, Kumar R, Lone AA, Rahimi M (2025) Mechanisms of low light stress in rice: current insights and future directions. Plant Growth Regul 105:1949-1968. https://doi.org/10.1007/s10725-025-01396-2

10.1007/s10725-025-01396-2
14

Evans JR, Poorter H (2001) Photosynthetic acclimation of plants to growth irradiance. Plant Cell Environ 24:755–767. https://doi.org/10.1046/j.1365-3040.2001.00724.x

10.1046/j.1365-3040.2001.00724.x
15

Fu W, Li P, Wu Y (2012) Effects of different light intensities on chlorophyll fluorescence characteristics and yield in lettuce. Sci Hortic 135:45-51. https://doi.org/10.1016/j.scienta.2011.12.004

10.1016/j.scienta.2011.12.004
16

Hazrati S, Tahmasebi-Sarvestani Z, Modarres-Sanavy AM, Mokhtassi-Bidgoli A, Nicola S (2016) Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L. Plant Physiol Biochem 106:141-148. https://doi.org/10.1016/j.plaphy.2016.04.046

10.1016/j.plaphy.2016.04.046
17

Kim HJ, Lee JS, Choi K, Kim J (2012) Growth and flowering responses of Calanthe discolor native to Korea under different light regimes. Flower Res J 20:71-74

18

Kim TJ, Kim JH, Lee JW, Lee CH, Paek KY (2001) Effect of different shading on CO2 uptake and growth and development of Dendrobium phalaenopsis. J Korean Soc Hortic Sci 42:111-115

19

Korea National Arboretum (2021) The national red list of vascular plants in Korea. Korea National Arboretum, Pocheon

20

Kozlowski TT, Kramer PJ, Pallardy SG (1991) The physiological ecology of woody plants. Academic Press, New York

10.1016/B978-0-12-424160-2.50005-7
21

Krishna SN, van Iersel MW (2004) Acclimation of wax begonia to light intensity: Changes in photosynthesis, respiration, and chlorophyll concentration. J Amer Soc Hortic Sci 129:745-751. https://doi.org/10.21273/JASHS.129.5.745

10.21273/JASHS.129.5.745
22

Lee DS, Yae BW, Lee YB, Lee YR (2011) Effect of shading level on the induction of inflorescence and growth of Phalaenopsis hybrid. Flower Res J 19:37-41

23

Lee JH, Nam SY (2024) Growth and photosynthetic responses of Taraxacum coreanum Nakai seedlings according to the shading levels. Hortic Sci Technol 42:214-224. https://doi.org/10.7235/HORT.20240018

10.7235/HORT.20240018
24

Lee JH, Yoo HJ, Nam SY (2022) Chlorophyll fluorescence response of indoor foliage plants as affected by light intensity levels under high temperature and continuous lighting conditions. J Agric Life Sci 56:19-26. https://doi.org/10.14397/jals.2022.56.1.19

10.14397/jals.2022.56.1.19
25

Lee SJ, Kim TW, Eom TS, Yoon TJ, Hwang YB, Yoo SY (2025) Identification of chlorophyll fluorescence parameters for evaluating drought stress in garden plants. J People Plants Environ 28:601-613. https://doi.org/10.11628/ksppe.2025.28.5.601

10.11628/ksppe.2025.28.5.601
26

Lee SK, Choi CS, Lee JG, Jang YA, Lee HJ, Chae WB, Do KR (2014) Influence of shading and irrigation on the growth and development of leaves tissue in hot pepper. Kor J Hort Sci Technol 32:448-453. http://doi.org/10.7235/hort.2014.14015

10.7235/hort.2014.14015
27

Mathur S, Jajoo H, Mehta P, Bharti S (2011) Analysis of elevated temperature-induced inhibition of photosystem II using chlorophyll a fluorescence induction kinetics in wheat leaves (Triticum aestivum). Plant Biol 13:1-6. https://doi.org/10.1111/j.1438-8677.2009.00319.x

10.1111/j.1438-8677.2009.00319.x
28

Maxwell K, Johnson GN (2000) Chlorophyll fluorescence-a practical guide. J Exp Bot 51:659-668. https://doi.org/10.1093/jexbot/51.345.659

10.1093/jexbot/51.345.659
29

Nakai T (1935) Coreanomecon hylomeconoides Nakai. J Jap Bot 11:151

30

Niinemets Ü (2010) Responses of forest trees to single and multiple environmental stresses from seedlings to mature plants: Past stress history, stress interactions, tolerance and acclimation. Forest Ecol Manage 260:1623–1639. https://doi.org/10.1016/j.foreco.2010.07.054

10.1016/j.foreco.2010.07.054
31

Oh S, Moon KH, Son IC, Song EY, Moon YE, Koh SC (2014) Growth, photosynthesis and chlorophyll fluorescence of Chinese cabbage in response to high temperature. Kor J Hortic Sci Technol 32:318-329. http://doi.org/10.7235/hort.2014.13174

10.7235/hort.2014.13174
32

Osmond CB (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In: Baker NR and Bowyer JR (Eds) Photoinhibition of photosynthesis, BIOS Scientific Publishers, Oxford, pp 1-24

33

Oukarroum A, Madidi SE, Schansker G, Strasser RJ (2007) Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. Environ Exp Bot 60:438-446

10.1016/j.envexpbot.2007.01.002
34

Parihar S, Soni V (2016) Biophysical characterization of drought tolerance in wheat (Triticum aestivum L.) through polyphasic chlorophyll fluorescence OJIP analysis. Biotechnol Res 2:48-52. https://doi.org/10.1016/j.envexpbot.2007.01.002

10.1016/j.envexpbot.2007.01.002
35

Peñuelas J, Baret F, Filella I (1995) Semi-empirical indices to assess carotenoids/chlorophyll a ratio from leaf spectral reflectance. Photosynthetica 31:221-230

36

Peñuelas J, Gamon JA, Fredeen AL, Merino J, Field CB (1994) Reflectance indices associated with physiological changes in nitrogen- and water-limited sunflower leaves. Remote Sens Environ 48:135-146. https://doi.org/10.1016/0034-4257(94)90136-8

10.1016/0034-4257(94)90136-8
37

Rhie YH, Lee SY, Jung HH, Kim KS (2014) Light intensity influences photosynthesis and crop characteristics of Jeffersonia dubia. Korean J Hortic Sci Technol 32:584-589. http://doi.org/10.7235/hort.2014.14028

10.7235/hort.2014.14028
38

Roh YS, Kim IK, Yoo YK (2020) Effects of dry and wet shipping conditions on quality, vase life, and physiological responses of Chrysanthemum morifolium ‘Baekma’ cut flowers. Hortic Sci Technol 38:218-227. https://doi.org/10.7235/HORT.20200021

10.7235/HORT.20200021
39

Roh YS, Yoo YK (2023) Light quality of light emitting diodes affects growth, chlorophyll fluorescence and phytohormones of tulip ‘Lasergame’. Hortic Environ Biotechnol 64: 245-255. https://doi.org/10.1007/s13580-022-00481-z

10.1007/s13580-022-00481-z
40

Semchenko M, Lepik M, Gotzenberger L, Zobel K (2012) Positive effect of shade on plant growth: amelioration of stress or active regulation of growth rate? J Ecol 100:459-466. https://doi.org/10.1111/j.1365-2745.2011.01936.x

10.1111/j.1365-2745.2011.01936.x
41

Seo SY, Kim CS, Kim HJ, Kim JM, Choi DC, Choi YG (2002) Characteristics of growth and flowering by shading and growth regulators in the lily of the valley (Convallaria keiskei M.). J Korean Soc Hortic Sci 43:644-648

42

Sessa G, Carabelli M, Possenti M, Morelli G, Ruberti I (2018) Multiple pathways in the control of the shade avoidance response. Plants 7:102. https://doi.org/10.3390/plants7040102

10.3390/plants704010230453622PMC6313891
43

Shin YK, Bhandari1 SR, Cho MC, Lee JG (2020) Evaluation of chlorophyll fluorescence parameters and proline content in tomato seedlings grown under different salt stress conditions. Hortic Environ Biotechnol 61:433-443. https://doi.org/10.1007/s13580-020-00231-z

10.1007/s13580-020-00231-z
44

Shin YK, Lee JG (2025) Differential physiological and growth responses of lettuce seedlings to extreme thermal stress analyzed through chlorophyll fluorescence. Hortic Sci Technol 43:604-622. https://doi.org/10.7235/HORT.20250055

10.7235/HORT.20250055
45

Son SW, Chung JM, Kim EH, Choi KS, Park SJ (2013) Genetic diversity and structure of the Korean endemic species, Coreanomecon hylomeconoides Nakai, as revealed by ISSR markers. Korean J Plant Res 26:310-319. http://doi.org/10.7732/kjpr.2013.26.2.310

10.7732/kjpr.2013.26.2.310
46

Son SW, Chung JM, Shin JK, Lee BC, Park KW, Park SJ (2012) Distribution, vegetation characteristics and assessment of the conservation status of a rare and endemic plant, Coreanomecon hylomeconoides Nakai. Korean J Plant Taxon 42:116-125

10.11110/kjpt.2012.42.2.116
47

Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Sharkey, Eaton-Rye JJ (Eds.) Advances in photosynthesis and respiration, Springer, New York, pp 321–362

10.1007/978-1-4020-3218-9_12
48

Valladares F, Niinemets U (2008) Shade tolerance, a key plant feature of complex nature and consequences. Annu Rev Ecol Evol Syst 39:237-57. https://doi.org/10.1146/annurev.ecolsys.39.110707.173506

10.1146/annurev.ecolsys.39.110707.173506
49

Wahid A, Gelani S, Ashraf M, Foolad MR (2007) Heat tolerance in plants: An overview. Environ Exp Bot 61:199-223. http://doi.org/10.1016/j.envexpbot.2007.05.011

10.1016/j.envexpbot.2007.05.011
50

Ye ZP (2007) A new model for relationship between irradiance and the rate of photosynthesis in Oryza sativa. Photosynthetica 45:637-640. http://doi.org/10.1007/s11099-007-0110-5

10.1007/s11099-007-0110-5
51

Yoo YK (2026) Growth, chlorophyll fluorescence, and mineral nutrient content of Pseudolysimachion nakaianum under different nitrogen fertilization levels. J People Plants Environ 29:59-67. https://doi.org/10.11628/ksppe.2026.29.1.59

10.11628/ksppe.2026.29.1.59
52

Zeiger E, Møller IM, Murphy A (2015) Plant physiology and development. Sinauer Associates, Inc, Massachusetts

53

Zushi K, Kajiwara S, Matsuzoe N (2012) Chlorophyll a fluorescence OJIP transient as a tool to characterize and evaluate response to heat and chilling stress in tomato leaf and fruit. Sci Hortic 148:39-46. http://doi.org/10.1016/j.scienta.2012.09.022

10.1016/j.scienta.2012.09.022
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Received Date : 2026-06-29
  • Revised Date : 2026-07-26
  • Accepted Date : 2026-08-13
  • DOI :https://doi.org/10.7235/HORT.20260032
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