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Genome-Wide Comparative Analysis of MYB Transcription Factors in Vitaceae and Expression Profiling Following Powdery Mildew Infection

포도과 MYB 전사인자의 유전체 수준 비교 분석 및 흰가루병 감염에 따른 발현 분석

Hye Jeong Cho1†
,
Dogyun Lee1†
,
Dahyun Yun1
,
Seo-Jin Kim1
,
Seungill Kim1*
조 혜정1†
,
이 도균1†
,
윤 다현1
,
김 서진1
,
김 승일1*
1Department of Environmental Horticulture, University of Seoul, Seoul 02504, Korea
1서울시립대학교 환경원예학과
*Corresponding Author
†These authors contributed equally to this work.
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Abstract

MYB transcription factors regulate a wide range of developmental, metabolic, and stress-responsive processes in plants. However, the genomic diversity and evolutionary patterns of these factors remain poorly characterized across Vitaceae species. We re-annotated MYB genes in five Vitaceae species (Cissus quadrangularis, Vitis amurensis, V. rotundifolia, V. vinifera, and V. piasezkii) and in Arabidopsis thaliana. A total of 1,891 MYB genes were identified, including 191 newly annotated genes that were missing from existing annotations. Among the identified genes, 1R-MYB and 2R-MYB were the two predominant repeat types, accounting for 51.5% and 46.4% of the total, respectively. A sequence conservation analysis revealed characteristic features of the MYB domain, including three predicted α-helices and highly conserved tryptophan residues, while a motif analysis showed distinct motif compositions between the first and second MYB repeats. A phylogenetic analysis classified the MYB genes into ten subgroups, with lineage-specific differences observed in gene expansion. Expression profiling of V. piasezkii following powdery mildew infection revealed distinct expression patterns among MYB genes. A co-expression network analysis further identified VPIA_67 and VPIA_312 as candidate MYB genes co-expressed with pathogen-response-related genes. These findings provide an updated characterization of MYB transcription factors in Vitaceae and identify candidate genes potentially involved in the powdery mildew response, offering a useful basis for both future functional studies and grape breeding.

Keywords
expression analysis
grapevine
pathogen response
re-annotation
repeat domain architecture

MYB 전사인자는 식물의 생장과 발달, 대사 과정 및 스트레스 반응을 조절하는 중요한 전사인자군이다. 그러나 포도과(Vitaceae)에서 MYB 유전자의 유전체적 다양성과 진화 양상은 아직 충분히 밝혀지지 않았다. 본 연구에서는 포도과 5종(Cissus quadrangularis, Vitis amurensis, V. rotundifolia, V. vinifera, V. piasezkii)과 애기장대(Arabidopsis thaliana)를 대상으로 MYB 유전자를 재동정하였다. 그 결과 총 1,891개의 MYB 유전자가 확인되었으며 이 중 191개는 기존 주석에서 누락되어 새롭게 동정된 유전자였다. 동정된 유전자 중 1R-MYB와 2R-MYB는 각각 전체의 51.5%와 46.4%를 차지하여 두 주요 반복 유형으로 나타났다. MYB 도메인의 서열 보존성 분석에서는 세 개의 예측된 α-helix와 높은 보존도를 보이는 트립토판 잔기 등 특징적인 MYB 도메인 특성이 확인되었으며 모티프 분석에서는 첫 번째와 두 번째 MYB 반복 사이에 서로 다른 모티프 구성이 나타났다. 계통 분석을 통해 MYB 유전자를 10개의 서브그룹으로 분류하였으며 유전자 확장 양상은 계통에 따라 차이를 보였다. V. piasezkii를 대상으로 흰가루병 감염 후 발현 분석을 수행한 결과 MYB 유전자들은 서로 다른 발현 양상을 나타냈다. 공동 발현 네트워크 분석에서는 병원균 반응 관련 유전자들과 공동 발현하는 VPIA_67과 VPIA_312가 MYB 후보 유전자로 확인되었다. 이러한 결과는 포도과 MYB 전사인자에 대한 정보를 보완하고 흰가루병 반응에 관여할 가능성이 있는 후보 유전자를 제시함으로써 향후 기능 연구와 포도 육종을 위한 기초 자료를 제공한다.

키워드
반복 도메인
병원균 반응
유전자 발현 분석
유전자 재동정
포도
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Received Date : 2026-08-27
  • Revised Date : 2026-09-02
  • Accepted Date : 2026-09-03
  • DOI :https://doi.org/10.7235/HORT.20260034
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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