All Issue

2025 Vol.43, Issue 5 Preview Page

Research Article

Effects of an Exogenous Trehalose Treatment on Ever-bearing Strawberry Cultivation during the High-Temperature Season

사계성 딸기 고온기 재배시 외인성 트레할로스 토양관주 처리의 효과

Jun-Hyung Lee1†
,
So Young Yi2†
,
Si-Yong Kang12*
이 준형1†
,
이 소영2†
,
강 시용12*
1Department of Horticulture, Kongju National University, Yesan 32439, Korea
2Research Center of Crop Breeding for Omics and Artificial Intelligence, Kongju National University, Yesan 32439, Korea
1국립공주대학교 원예학과
2국립공주대학교 작물오믹스지능육종연구센터
*Corresponding Author
†These authors contributed equally to this work.
31 October 2025. pp. 660-672
PDF XML Citation
Abstract

The hot and humid conditions during the summer in South Korea can hinder growth, and diseases become a factor as well with regard to reduced fruit productivity and lower quality of ever-bearing strawberry plants. This study explores trehalose, a non-reducing disaccharide known for its regulatory roles in plant stress responses, as an eco-friendly treatment to improve strawberry productivity under high-temperature conditions. Ever-bearing strawberries were irrigated with trehalose solutions at 0, 10, and 30 mM concentrations weekly for seven weeks. The results showed no significant differences in growth, fruit quality, or photosynthetic efficiency. However, trehalose affected soil conditions, with the soil electrical conductivity (EC) decreasing as the concentration of treated trehalose increased. By the fourth week, EC in the 30 mM treatment group was lower (0.94 dS·m-1) than in the control (1.56 dS·m-1). This trend suggests that trehalose improves the soil. Additionally, the trehalose treatment reduced leaf tip burn symptoms in a concentration-dependent manner. By week six, the 30 mM treatment group displayed fewer affected leaves (2.2 per plant) than the control group (4.2 leaves), showing nearly a 50% reduction. The findings here indicate that trehalose can improve the soil EC and mitigate leaf tip burn symptoms, enhancing plant resilience. This suggests the potential of trehalose as a sustainable treatment for managing stress during strawberry cultivation, warranting further explorations into broader agricultural applications of this disaccharide.

Keywords
abiotic stress
eco-friendly culture
Fragaria × ananassa
growth disorder
rhizosphere

한국의 여름철 고온 다습한 환경은 딸기 생장에 장애를 주고 병해를 유발하여 생산성 및 품질 저하를 초래할 수 있다. 본 연구는 식물의 스트레스 반응 조절에 중요한 역할을 하는 비환원성 이당류인 트레할로스 처리를 통한 고온 환경하 딸기 재배시에 스트레스 경감을 위한 친환경제제로서의 이용 가능성을 탐구하고자 하였다. 사계성 딸기 ‘W-331’ 를 여름철에 유리온실내에서 상토를 채운 포트에 재배하고, 0(대조구), 10, 30mM 농도의 트레할로스 용액을 매주 2L씩 7주간 관주하여 처리하였다. 연구 결과, 트레할로스 처리에 따른 딸기의 생장, 과일 품질 및 광합성 효율 등에는 유의미한 차이가 없었다. 그러나 트레할로스는 토양 상태에 영향을 미쳤으며, 트레할로스 농도가 증가할수록 토양 전기전도도(EC)가 감소하는 경향을 보였다. 4주차에는 30mM 농도의 처리군에서 EC 값이 대조구(1.56dS·m-1)에 비해 낮은 0.94dS·m-1을 기록했다. 이는 트레할로스가 토양 물리적 특성 개선에 기여할 수 있음이 시사되었다. 또한, 트레할로스 처리에 의해 딸기 잎끝마름 증상이 농도 의존적으로 감소하였으며, 6주차에는 30mM 처리군에서 잎끝마름 증상을 보인 잎 수가 대조구(4.2개)에 비해 약 50% 감소한 2.2개로 나타났다. 이러한 결과는 트레할로스 30mM를 관주처리하면, 토양 EC를 개선하고 잎끝마름 증상을 완화시켜 식물의 내성 향상에 기여할 수 있고, 시들음병 증상을 완화하는 것을 나타낸다. 따라서 트레할로스 처리가 딸기 재배에서 고온 스트레스 경감을 위해 활용될 수 있는 것으로 보여져, 향후 구체적인 작용 기작 구명과 활용 방안 연구가 필요하다고 생각된다.

키워드
비생물적스트레스
친환경재배
재배딸기
생장장애
근권환경
References
1

Ali Q, Ashraf M, Anwar F, Al-Qurainy F (2012) Trehalose-induced changes in seed oil composition and antioxidant potential of maize grown under drought stress. J Am Oil Chem Soc 89:1485-1493. https://doi.org/10.1007/s11746-012-2032-z

10.1007/s11746-012-2032-z
2

Britto SD, Joshi SM, Jogaiah S (2021) Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator. Biotechnol Rep 32:e00690. https://doi.org/10.1016/j.btre.2021.e00690

10.1016/j.btre.2021.e0069034987982PMC8711064
3

Choi HG, Moon BY, Kang NJ (2016) Correlation between strawberry (Fragaria ananassa, Duch.) productivity and photosynthesis-related parameters under various growth conditions. Front Plant Sci 7. https://doi.org/10.3389/fpls.2016.01607

10.3389/fpls.2016.01607
4

Choi JH (2023) Agricultural area survey in 2023. In. Statistics Korea

5

Choi SH, Choi K, Jeong HJ, Kim SH, Lee SC, Choi HG (2017) Optimum nutrient concentration to improve growth and quality of strawberry cultivars 'Berrystar' and 'Jukhyang' in Hydroponics. J Bio-Env Con 26:424-431. https://doi.org/10.12791/KSBEC.2017.26.4.424

10.12791/KSBEC.2017.26.4.424
6

Fernandez O, Bethencourt L, Quero A, Sangwan RS, Clement C (2010) Trehalose and plant stress responses: friend or foe?. Trends Plant Sci 15:409-417. https://doi.org/10.1016/j.tplants.2010.04.004

10.1016/j.tplants.2010.04.00420494608
7

Govind SR, Jogaiah S, Abdelrahman M, Shetty HS, Tran LS (2016) Exogenous trehalose treatment enhances the activities of defense-related enzymes and triggers resistance against downy mildew disease of pearl millet. Front Plant Sci 7:1593. https://doi.org/10.3389/fpls.2016.01593

10.3389/fpls.2016.0159327895647PMC5109038
8

Hu Y, Han YT, Wei W, Li YJ, Zhang K, Gao YR, Zhao FL, Feng JY (2015) Identification, isolation, and expression analysis of heat shock transcription factors in the diploid woodland strawberry Fragaria vesca. Front Plant Sci 6:736. https://doi.org/10.3389/fpls.2015.00736

10.3389/fpls.2015.00736
9

Kadir S, Sidhu G, Al-Khatib K (2006) Strawberry (Fragaria ×ananassa Duch.) growth and productivity as affected by temperature. HortScience 41:1423-1430. https://doi.org/10.21273/HORTSCI.41.6.1423

10.21273/HORTSCI.41.6.1423
10

Kandler O, Hopf H (1980) 7 - Occurrence, metabolism, and function of oligosaccharides. Elsevier 221-270. https://doi.org/10.1016/B978-0-12-675403-2.50013-2

10.1016/B978-0-12-675403-2.50013-2
11

Kang SB, Lee IB, Park JM, Lim TJ (2010) Effect of waterlogging conditions on the growth, root activities and nutrient content of 'Campbell Early' grapevine. Hortic Sci Technol 28:172-179

12

Kitajima M, Butler WL (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim Biophys Acta Bioenerg 376:105-115. https://doi.org/10.1016/0005-2728(75)90209-1

10.1016/0005-2728(75)90209-1
13

Kosar F, Akram NA, Sadiq M, Al-Qurainy F, Ashraf M (2019) Trehalose: A key organic osmolyte effectively involved in plant abiotic stress tolerance. J Plant Growth Regul 38:606-618. https://doi.org/10.1007/s00344-018-9876-x

10.1007/s00344-018-9876-x
14

Krasensky J, Jonak C (2012) Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. J Exp Bot 63:1593-1608. https://doi.org/10.1093/jxb/err460

10.1093/jxb/err46022291134PMC4359903
15

Lee GB, Choe YU, Park YH, Choi YW, Kang JS (2018) Effects of groundwater cooling treatment on growth, yield, and quality of strawberries under high temperature conditions. J Environ Sci Int 27:631-639. https://doi.org/10.5322/JESI.2018.27.8.631

10.5322/JESI.2018.27.8.631
16

Lee JN, Kim HJ, Kim KD, Kwen KB, Suh JT (2017) Characteristics of New Ever-bearing Strawberry 'Jangha' Bred for High Soluble Solids Contents. Korean J Hortic Sci Technol 35:381-386. https://doi.org/10.12972/kjhst.20170040

10.12972/kjhst.20170040
17

Lee JN, Kim HJ, Kim KD, Yoo DL, Suh JT (2014) Characteristics of the new ever-bearing strawberry 'Yeolha' for high yield. Hortic Sci Technol 32:739-743. https://doi.org/10.7235/hort.2014.14069

10.7235/hort.2014.14069
18

Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Current Protocols in Food Analytical Chemistry 1:F4.3.1-F4.3.8. https://doi.org/10.1002/0471142913.faf0403s01

10.1002/0471142913.faf0403s01
19

Liu TA, Lin JJ, Wu RY (2006) The effects of using trehalose as a carbon source on the proliferation of Phalaenopsis and Doritaenopsis protocorm-like-bodies. Plant Cell Tissue Organ Cult 86:125-129. https://doi.org/10.1007/s11240-006-9092-4

10.1007/s11240-006-9092-4
20

Lu XY, Xu H, Song W, Yang ZT, Yu J, Tian YE, Jiang M, Shen DY, Dou DL (2021) Rapid and simple detection of in strawberry using a coupled recombinase polymerase amplification-lateral flow strip assay. Phytopathol Res 3. https://doi.org/10.1186/s42483-021-00089-8

10.1186/s42483-021-00089-834127941PMC8189726
21

Lunn JE, Delorge I, Figueroa CM, Van Dijck P, Stitt M (2014) Trehalose metabolism in plants. Plant J 79:544-567. https://doi.org/10.1111/tpj.12509

10.1111/tpj.1250924645920
22

Luo Y, Wang W, Fan YZ, Gao YM, Wang D (2018) Exogenously-supplied trehalose provides better protection for d1 protein in winter wheat under heat stress. Russ J Plant Physiol 65:115-122. https://doi.org/10.1134/S1021443718010168

10.1134/S1021443718010168
23

Luo Y, Wang Y, Xie YY, Gao YM, Li WQ, Lang SP (2022) Transcriptomic and metabolomic analyses of the effects of exogenous trehalose on heat tolerance in wheat. Int J Mol Sci 23. https://doi.org/10.3390/ijms23095194

10.3390/ijms2309519435563585PMC9103215
24

Luo Y, Xie Y, He D, Wang W, Yuan S (2021) Exogenous trehalose protects photosystem II by promoting cyclic electron flow under heat and drought stresses in winter wheat. Plant Biol (Stuttg) 23:770-776. https://doi.org/10.1111/plb.13277

10.1111/plb.1327733914400
25

MacIntyre AM, Meline V, Gorman Z, Augustine SP, Dye CJ, Hamilton CD, Iyer-Pascuzzi AS, Kolomiets MV, McCulloh KA, et al. (2022) Trehalose increases tomato drought tolerance, induces defenses, and increases resistance to bacterial wilt disease. PLoS One 17. https://doi.org/10.1371/journal.pone.0266254

10.1371/journal.pone.026625435476629PMC9045674
26

Mori IC, Matsuura T, Otao M, Ooi L, Nishimura Y, Hirayama T (2023) Application of trehalose mitigates short-styled flowers in solanaceous crops. J Agric Food Chem 71:5476-5482. https://doi.org/10.1021/acs.jafc.2c08479

10.1021/acs.jafc.2c0847937011406PMC10103160
27

Na YW, Jeong H, Lee SY, Choi HG, Kim SH, Rho IR (2014) Chlorophyll fluorescence as a diagnostic tool for abiotic stress tolerance in wild and cultivated strawberry species. Hortic Environ Biotechnol 55:280-286. https://doi.org/10.1007/s13580-014-0006-9

10.1007/s13580-014-0006-9
28

Nam MH, Kim HS, Kim TI, Lee EM (2015) Comparison of environmental-friendly and chemical spray calendar for controlling diseases and insect pests of strawberry during nursery seasons. Res Plant Dis 21:273-279. https://doi.org/10.5423/RPD.2015.21.4.273

10.5423/RPD.2015.21.4.273
29

Nam MH, Kim HS, Park MS, Min JY, Kim HT (2020) Genetic diversity, pathogenicity, and fungicide response of Fusarium oxysporum f. sp. fragariae isolated from strawberry plants in Korea. Res Plant Dis 26:79-87. https://doi.org/10.5423/RPD.2020.26.2.79

10.5423/RPD.2020.26.2.79
30

Ohtake S, Wang YJ (2011) Trehalose: current use and future applications. J Pharm Sci 100:2020-2053. https://doi.org/10.1002/jps.22458

10.1002/jps.2245821337544
31

Paul MJ, Primavesi LF, Jhurreea D, Zhang Y (2008) Trehalose metabolism and signaling. Annu Rev Plant Biol 59:417-441. https://doi.org/10.1146/annurev.arplant.59.032607.092945

10.1146/annurev.arplant.59.032607.09294518257709
32

Paul S, Paul S (2014) Trehalose induced modifications in the solvation pattern of N-Methylacetamide. J Phys Chem B 118:1052-1063. https://doi.org/10.1021/jp407782x

10.1021/jp407782x24423002
33

Reignault PH, Cogan A, Muchembled J, Lounes-Hadj Sahraoui A, Durand R, Sancholle M (2001) Trehalose induces resistance to powdery mildew in wheat. New Phytol 149:519-529. https://doi.org/10.1046/j.1469-8137.2001.00035.x

10.1046/j.1469-8137.2001.00035.x33873340
34

Ruan J, Lee YH, Hong SJ, Yeoung YR (2013) Sugar and organic acid contents of day-neutral and ever-bearing strawberry cultivars in high-elevation for summer and autumn fruit production in Korea. Hortic Environ Biotechnol 54:214-222. https://doi.org/10.1007/s13580-013-0186-8

10.1007/s13580-013-0186-8
35

Ryou Y, Kang Y, Kim Y, Kang K (2008) Heating and cooling effect of portected horticulture by geothermal heat pump system with horizontal heat exchanger. In forum on renewable energy, Vol 2008.05a. Korean Society for New and Renewable Energy, pp 630-633

36

Samadi S, Habibi G, Vaziri A (2019) Exogenous trehalose alleviates the inhibitory effects of salt stress in strawberry plants. Acta Physiol Plant 41. https://doi.org/10.1007/s11738-019-2905-y

10.1007/s11738-019-2905-y
37

Schluepmann H, van Dijken A, Aghdasi M, Wobbes B, Paul M, Smeekens S (2004) Trehalose mediated growth inhibition of Arabidopsis seedlings is due to trehalose-6-phosphate accumulation. Plant Physiol 135:879-890. https://doi.org/10.1104/pp.104.039503

10.1104/pp.104.03950315181209PMC514123
38

Singh V, Louis J, Ayre BG, Reese JC, Pegadaraju V, Shah J (2011) TREHALOSE PHOSPHATE SYNTHASE11-dependent trehalose metabolism promotes Arabidopsis thaliana defense against the phloem-feeding insect Myzus persicae. Plant J 67:94-104. https://doi.org/10.1111/j.1365-313X.2011.04583.x

10.1111/j.1365-313X.2011.04583.x21426427
39

Wahid A, Gelani S, Ashraf M, Foolad MR (2007) Heat tolerance in plants: An overview. Environ Exp Bot 61:199-223. https://doi.org/10.1016/j.envexpbot.2007.05.011

10.1016/j.envexpbot.2007.05.011
40

Yoo JH, Park MS, Yi DH, Nam MH (2024) First report of anthracnose caused by colletotrichum nymphaeae on strawberry fruits in Korea. Kor J Mycol 52:109-114

41

Zhao DQ, Li TT, Hao ZJ, Cheng ML, Tao J (2019) Exogenous trehalose confers high temperature stress tolerance to herbaceous peony by enhancing antioxidant systems, activating photosynthesis, and protecting cell structure. Cell Stress Chaperones 24:247-257. https://doi.org/10.1007/s12192-018-00961-1

10.1007/s12192-018-00961-130632065PMC6363623
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :5
  • Pages :660-672
  • Received Date : 2024-07-30
  • Revised Date : 2024-12-03
  • Accepted Date : 2024-12-31
  • DOI :https://doi.org/10.7235/HORT.20250034
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
Online Submission
  • scopus
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close