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2022 Vol.40, Issue 5 Preview Page

Research Article

Role of Phenolic Compounds in Root Induction of Virus-free Apple Rootstocks and Scions
Jong-Eun Han1†
,
Bo-Min Kwon1†
,
Hosakatte Niranjana Murthy12
,
Cheol-Seung Jeong13
,
So-Young Park1*
1Department of Horticulture, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28466, Korea
2Department of Botany, Karnatak University, Dharwad 580003, India
3BIO-FD&C Co., Ltd., Incheon 21990, Korea
*Corresponding Author
†These authors contributed equally to this work.
31 October 2022. pp. 561-570
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Abstract

The objective of the current study was to identify and quantify endogenous phenolic compounds in easy-to-root M.9 and M.26 rootstocks and difficult-to-root cultivars ‘Hongro’ (HR) and ‘Fuji’ (FJ) (scions) of apple (Malus domestica Borkh.) during in vitro rooting of shoots for 40 days in culture. Phloretic acid, coumaric acid, salicylic acid, chlorogenic acid, catechin hydrate, hesperetin, rutin, quercetin, and biochanin were the major endogenous phenolic compounds detected in the apple shoots by high-performance liquid chromatography (HPLC). Among the various phenolic compounds, chlorogenic acid was most abundant in shoots, favoring the induction and growth of adventitious roots from in vitro shoots. In contrast, salicylic acid was another phenolic acid that was reduced during the culture of shoots in the easy-to-root rootstocks (M.9 and M.26). However, salicylic acid levels remained constant or increased throughout the culture in the difficult-to-root cultivars (HR and FJ). This study indicates the role of endogenous phenolic compounds on the rooting of shoots and lays the foundation for further studies investigating the mechanism of rooting in these apple rootstocks and cultivars.

Keywords
chlorogenic acid
in vitro rooting
indole-3-butyric acid
Malus domestica
salicylic acid
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 40
  • No :5
  • Pages :561-570
  • Received Date : 2022-06-23
  • Revised Date : 2022-07-13
  • Accepted Date : 2022-07-28
  • DOI :https://doi.org/10.7235/HORT.20220050
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