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2024 Vol.42, Issue 6 Preview Page

Research Article

Effects of Vermicompost Combined with Exogenous Silicon or Salicylic Acid on the Physiological and Photosynthetic Characteristics of Muskmelon Seedlings in Moderately Saline Soil
Cheng-Cheng Zeng1
,
Li-Rong Su1*
,
Fang Qin1
,
Qin Li1
,
Jin-Ping Chen1
,
Tian-Ming Su1
,
Yu-Mo Tan1
,
Tie-Guang He1
1Agricultural Resources and Environmental Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Arable Land Conservation, Nanning 530007, China
*Corresponding Author
31 December 2024. pp. 667-677
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Abstract

Soil salinization has become a major obstacle to sustainable melon production, and single-measure approaches have failed to improve crop yields and quality outcomes. In this study, we explore the effects and underlying mechanisms of a vermicompost (VC) treatment combined with silicon (Si) or salicylic acid (SA) on salt damage to muskmelon seedlings growing in moderately saline soil collected from a greenhouse. We evaluated the effects of four treatments: a control treatment (CK), 10% vermicompost (VC), 10% vermicompost combined with 0.04% Si (VC+Si), and 10% vermicompost combined with 100 µmol/L salicylic acid (VC+SA). We found that vermicompost could alleviate salt damage and that the combination of vermicompost with exogenous Si or SA could amplify this effect. Compared to CK, plant height and shoot biomass were both greater when vermicompost was applied in combination with SA or Si. A chlorophyll fluorescence analysis indicated that a VC treatment can reduce damage to the PSII reaction center caused by salt stress, whereas it did not have a significant effect on Fv/Fm, Fv'/Fm' or qP. The PSII activity increased significantly under VC+SA and VC+Si compared to CK. We did not measure significant differences in the stomatal conductance, intercellular CO2, or transpiration rate under VC relative to CK, but the VC+Si and VC+SA treatments increased the net photosynthetic rate and intercellular CO2. Superoxide anion and malondialdehyde (MDA) levels were lower across all treatments relative to CK. Moreover, compared to VC, superoxide anion and MDA contents decreased significantly when VC was combined with Si or SA. Superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities increased significantly under the VC+SA treatment compared to the VC treatment group. Proline, soluble sugar and protein contents were higher in all treatment groups than in CK, with the highest values observed in the VC+SA treatment group. Combined with Si or SA, a VC treatment can profoundly strengthen salt tolerance and improve plant growth compared to the application of VC alone.

Keywords
antioxidant enzyme
chlorophyll fluorescence
malondialdehyde
salt stress
superoxide dismutase
References
1

Afzal I, Basra SMA, Farooq M, Nawaz A (2006) Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid. Int J Agric Biol 8:23-28. doi:1560-8530/2006/08-1-23-28

2

Ali M, Afzal S, Parveen AKM, Javed M, Rizwan A, Ghulam H, Malik Z, Riaz M, Salman A, et al. (2021) Silicon mediated improvement in the growth and ion homeostasis by decreasing Na+ uptake in maize (Zea mays L.) cultivars exposed to salinity stress. Plant Physiol Bioch 158:208-218. doi:10.1016/j.plaphy.2020.10.040

10.1016/j.plaphy.2020.10.04033281032
3

Atiyeh RM, Subler S, Edwards CA, Bachman G, Metzger JD, Shuster W (2000) Effects of vermicomposts and composts on plant growth in horticultural container media and soil. Pedobiologia 44:579-590. doi:10.1078/S0031-4056(04)70073-6

10.1078/S0031-4056(04)70073-6
4

Bao SD (2000) Soil and agricultural chemidtry analysis. Beijing, China, China Agricultural University press, pp187

5

Bosnic P, Bosnic D, Jasnic J, Nikolic M (2018) Silicon mediates sodium transport and partitioning in maize under moderate salt stress. Environ Exp Bot 155:681-687. doi:10.1016/j.envexpbot.2018.08.018

10.1016/j.envexpbot.2018.08.018
6

Chao YY, Chen CY, Huang WD, Kao CH (2010) Salicylic acid-mediated hydrogen peroxide accumulation and protection against Cd toxicity in rice leaves. Plant Soil 329:327-337. doi:10.1007/s11104-009-0161-4

10.1007/s11104-009-0161-4
7

Edwards CA, Burrows I (1988) The potential of earthworm composts as plant growth media. Earthworms in waste and environment management. The Hague, The Netherlands, SPB Academic Press, pp 21-32

8

Fornes F, Mendoza-Hernández D, García-de-la-Fuente R, Abad M, Belda RM (2012) Composting versus vermicomposting: a comparative study of organic matter evolution through straight and combined processes. Bioresour Technol 118:296-305. doi:10.1016/j.biortech.2012.05.028

10.1016/j.biortech.2012.05.02822705537
9

Gadallah MAA, Sayed SA (2014) Impacts of different water pollution sources on antioxidant defense ability in three aquatic macrophytes in assiut province, Egypt. J Stress Physiol Biochem 10:47-50

10

Gou C, Huang Q, Rady MM, Wang L, Ihtisham M, El-Awady HH, Seif M, Alazizi EMY, Eid RS, et al. (2023) Integrative application of silicon and/or proline improves Sweet corn (Zea mays L. saccharata) production and antioxidant defense system under salt stress condition. Sci Rep 13:18315. doi:10.1038/s41598-023-45003-8

10.1038/s41598-023-45003-837880216PMC10600099
11

Gupta S, Gupta M (2016) Alleviation of selenium toxicity in Brassica juncea L.: salicylic acid-mediated modulation in toxicity indicators, stress modulators, and sulfur-related gene transcripts. Protoplasma 253:1515-1528. doi:10.1007/s00709-015-0908-0

10.1007/s00709-015-0908-026573535
12

Gurmani AR, Bano A, Najeeb U, Zhang J, Khan SU, Flowers TJ (2013) Exogenously applied silicate and abscisic acid ameliorates the growth of salinity stressed wheat ('Triticum aestivum' L) seedlings through Na+ exclusion. Aust J Crop Sci 7:1123-1130

13

Haghighi TM, Saharkhiz MJ, Ramezanian A, Zarei M (2023) The use of silicon and mycorrhizal fungi to mitigate changes in licorice leaf micromorphology, chlorophyll fluorescence, and rutin content under water-deficit conditions. Plant Physiol Biochem 197:107662. doi:10.1016/j.plaphy.2023.107662

10.1016/j.plaphy.2023.10766236989994
14

Hatzig S, Zaharia LI, Abrams S, Hohmann M, Legoahec L, Bouchereau A, Nesi N, Snowdon RJ (2014) Early osmotic adjustm ent responses in drought-resistant and drought-sensitive oilseed rape. J Integr Plant Biol 56:797-809. doi:10.1111/jipb.12199

10.1111/jipb.1219924667002
15

Horvath E, Brunner S, Bela K, Papdi C, Szabados L, Tari I, Csiszar J (2015) Exogenous salicylic acid-triggered changes in the glutathione transferases and peroxidases are key factors in the successful salt stress acclimation of Arabidopsis thaliana. Funct Plant Biol 42:1129-1140. doi:10.1071/FP15119

10.1071/FP1511932480751
16

Jini D, Joseph B (2017) Physiological mechanism of salicylic acid for alleviation of salt stress in rice. Rice Sci 24:97-108. doi:10.1016/j.rsci.2016.07.007

10.1016/j.rsci.2016.07.007
17

Kalaji HM, Jajoo A, Oukarroum A, Brestic M, Zivcak M, Samborska IA, Cetner MD, Lukasik I, Goltsev V (2016) Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions. Acta Physiol Plant 38:1-11. doi:10.1007/s11738-016-2113-y

10.1007/s11738-016-2113-y
18

Laifa I, Hajji M, Farhat N, Elkhouni A, Smaoui A, M'nif A, Hamzaoui AH, Savoure A, Abdelly C, et al. (2021) Beneficial effects of silicon (Si) on sea barley (Hordeum marinum Huds.) under salt stress. Silicon 13:4501-4517. doi:10.1007/s12633-020-00770-1

10.1007/s12633-020-00770-1
19

Li YF, Huang LL, Liu XL, Li XS, Tan HH (2020) Exogenous salicylic acid alleviates halosulfuron-methyl toxicity by coordinating the antioxidant system and improving photosynthesis in soybean (Glycine max Merr.). Acta Physiol Plant 42. doi:10.1007/s11738-020-03075-3

10.1007/s11738-020-03075-3
20

Li YP, Wang J, Shao MA (2021) Application of earthworm cast improves soil aggregation and aggregate-associated carbon stability in typical soils from loess plateau. J Environ Manage 15:278. doi:10.1016/j.jenvman.2020.111504

10.1016/j.jenvman.2020.11150433120095
21

Liang Y, Zhang W, Qin C, Liu Y, Ding R (2006) Effect of exogenous silicon (Si) on H+-ATPase activity, phospholipids and fluidity of plasma membrane in leaves of salt-stressed barley (Hordeum vulgare L.). Environ Exp Bot 57:212-219. doi:10.1016/j.envexpbot.2005.05.012

10.1016/j.envexpbot.2005.05.012
22

Liu B, Soundararajan P, Manivannan A (2019) Mechanisms of silicon-mediated amelioration of salt stress in plants. Plants 8:307. doi:10.3390/plants8090307

10.3390/plants809030731461994PMC6784176
23

Mohammed KA, Abd El-Rheem KHM, Elsawy AM, Essa EM (2018) Effect of vermicompost supplemented by foliar application of silicate on marjoram plants grown in saline soil. Int J Adv Sci Eng Inf Technol 8:1029-1035. doi:10.18517/ijaseit.8.4.5654

10.18517/ijaseit.8.4.5654
24

Nazar R, Iqbal N, Syeed S, Khan NA (2011) Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars. J Plant Physiol 168:807-815. doi:10.1016/j.jplph.2010.11.001

10.1016/j.jplph.2010.11.00121112120
25

Nazari Z, Sharifi RS, Narimani H (2022) Effect of Mycorrhiza, vermicompost and nano silicon on agronomic and physiological traits of triticale under different intensities of drought stress. Crop Prod J 14:21-46. doi:10.22069/EJCP.2022.18925.2413

10.22069/EJCP.2022.18925.2413
26

Norman C, Howell KA, Millar AH, Whelan JM, Day DA (2004) Salicylic acid is an uncoupler and inhibitor of mitochondrial electron transport. Plant Physiol 134:492-501. doi:10.1104/pp.103.031039

10.1104/pp.103.03103914684840PMC316328
27

Pan T, Dong Q, Cai Y, Cai K (2023) Silicon-mediated regulation of cadmium transport and activation of antioxidant defense system enhances Pennisetum glaucum resistance to cadmium stress. Plant Physiol Biochem, 195:206-213. doi:10.1016/j.plaphy.2023.01.021

10.1016/j.plaphy.2023.01.02136641944
28

Prabhakaran S, Abinaya M, Ho KC, Sowbiya M, Ryong JB (2017) Leaf physiological and proteomic analysis to elucidate silicon induced adaptive response under salt stress in rosa hybrida 'Rock Fire'. Int J Mol Sci 18:1768. doi:10.3390/ijms18081768

10.3390/ijms1808176828805727PMC5578157
29

Rizwan M, Ali S, Ibrahim M, Farid M, Adrees M, Bharwana SA, Abbas F (2015) Mechanisms of silicon-mediated alleviation of drought and salt stress in plants: a review. Environ Sci Pollut Res 22:15416-15431. doi:10.1007/s11356-015-5305-x

10.1007/s11356-015-5305-x26335528
30

Sahni S, Kumar S, Prasad BD (2021) Integration of salicylic acid, vermicompost and bioagent for effective management of chickpea wilt disease. J Environ Biol 42:1274-1280. doi:10.22438/jeb/42/5/MRN-1745

10.22438/jeb/42/5/MRN-1745
31

Sako K, Nguyen HM, Seki M (2020) Advances in chemical priming to enhance abiotic stress tolerance in plants. Plant Cell Physiol 61:1995-2003. doi:10.1093/pcp/pcaa119

10.1093/pcp/pcaa11932966567
32

Shemi R, Wang R, Gheith E, Hussain HA, Cholidah L, Zhang KP, Zhang A, Wang LC (2021) Role of exogenous-applied salicylic acid, zinc and glycine betaine to improve drought-tolerance in wheat during reproductive growth stages. BMC Plant Biol 21:574. doi:10.1186/s12870-021-03367-x

10.1186/s12870-021-03367-x34872519PMC8647490
33

Song XF, Liu SS, Xia LQ, Gao P, Luan FS, Liu Z (2022) Advances of melon breeding technology and method. Chin Cucurbit Vegeta 35:1-8. doi:10.16861/j.cnki.zggc.2022.0151

10.16861/j.cnki.zggc.2022.0151
34

Souana K, Taïbi K, Abderrahim LA, Amirat M, Achir M, Boussaid M, Mulet JM (2020) Salt-tolerance in Vicia faba L. is mitigated by the capacity of salicylic acid to improve photosynthesis and antioxidant response. Sci Hortic 273:109641. doi:10.1016/j.scienta.2020.109641

10.1016/j.scienta.2020.109641
35

Souza Junior JP, Prado RM, Silva Campos CN, Sousa Junior GDS, Costa MG, de Pádua Teixeira S, Gratão PL (2023) Silicon modulate the non-enzymatic antioxidant defence system and oxidative stress in a similar way as boron in boron-deficient cotton flowers. Plant Physiol Biochem: PPB, 197:107594. doi:10.1016/j.plaphy.2023.02.024

10.1016/j.plaphy.2023.02.02437001302
36

Srivastava M, Ma LQ, Singh N, Singh S (2005) Antioxidant responses of hyper-accumulator and sensitive fern species to arsenic. J Exp Bot 56:1335-1342. doi:10.1093/jxb/eri134

10.1093/jxb/eri13415781440
37

Tasgin E, Atici O, Nalbantoglu B, Popova LP (2006) Effects of salicylicacid and cold treatments on protein levels and on the activities of antioxidant enzymes in the apoplast of winter wheat leaves. Phytochem 67:710-715. doi:10.1016/j.phytochem.2006.01.022

10.1016/j.phytochem.2006.01.02216519911
38

Uma B, Malathi M (2009) Vermicompost as a soil supplement to improve growth and yield of Amaranthus species. Res J Agric Biol Sci 5:1054-1060

39

Wang HM, Xiao XR, Yang MY, Gao ZL, Zang J, Fu XM, Chen Y (2014) Effects of salt stress on antioxidant defense system in the root of kandelia candel, Bot Stud 55:57. doi:10.1186/s40529-014-0057-3

10.1186/s40529-014-0057-328510976PMC5430347
40

Wang LJ, Fan L, Loescher W, Duan W, Guo JL, Cheng JS, Luo HB, Li SH (2010) Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves. BMC Plant Biol 10:34-40. doi:10.1186/1471-2229-10-34

10.1186/1471-2229-10-3420178597PMC2848757
41

Yin JL, Jia JH, Lian ZY, Guo YH, Huo HQ, Zhu YX, Gong HJ (2019) Silicon enhances the salt tolerance of cucumber through increasing polyamine accumulation and decreasing oxidative damage. Ecotox Environ Safe 169:8-17. doi:10.1016/j.ecoenv.2018.10.105

10.1016/j.ecoenv.2018.10.10530412897
42

Zelm EV, Zhang Y, Testerink C (2020) Salt tolerance mechanisms of plants. Annu Rev Plant Biol 71:403-433. doi:10.1146/annurev-arplant-050718-100005

10.1146/annurev-arplant-050718-10000532167791
43

Zhang M, Li Y, Ning P, Cheng XM, Huang XX (2022) Effects of applying exogenous NO with foliar and rhizosphere methods on physiological characteristics of Hedera nepalensis var. sinensis under salt stress. Acta Agric Univer Jiangxi 44:583-592

44

Zhu Y, Guo J, Feng R, Jia JH, Han WH, Gong HJ (2016) The regulatory role of silicon on carbohydrate metabolism in Cucumis sativus L. under salt stress. Plant Soil 406:231-249. doi:10.1007/s11104-016-2877-2

10.1007/s11104-016-2877-2
45

Zhu YX, Gong HJ, Yin JL (2019) Role of silicon in mediating salt tolerance in plants: a review. Plants 8:147. doi:10.3390/plants8060147

10.3390/plants806014731159197PMC6630593
46

Zhu YX, Jiang XC, Zhang J, Yang H, Zhu XM, Zhou XM, Zhou XK, Gong HJ, Yin HL, et al. (2020) Silicon confers cucumber resistance to salinity stress through regulation of proline and cytokinins. Plant Physiol Biochem 156:209-220. doi:10.1016/j.plaphy.2020.09.014

10.1016/j.plaphy.2020.09.01432977177
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 42
  • No :6
  • Pages :667-677
  • Received Date : 2024-01-11
  • Revised Date : 2024-04-13
  • Accepted Date : 2024-04-29
  • DOI :https://doi.org/10.7235/HORT.20240054
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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