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2021 Vol.39, Issue 4 Preview Page

Research Article

Identification of Dominant Microbial Community and Diversity in Continuously Cropped Pepper Fields
Hui Wang1
,
Li Liu1
,
Shuyi Yu1
,
Tianshu Guan1
,
Baihong Li1
,
Qi Wang2
,
Changyuan Liu1*
1Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China
2Department of Plant Pathology, China Agricultural University, Beijing 100193, China
*Corresponding Author
31 August 2021. pp. 431-445
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Abstract

Pepper blight is the most significant soil-borne disease affecting the continuous cropping of peppers. To identify the effect of Phytophthora capsici infection on microbial flora, we isolated and counted the microorganisms collected from the rhizosphere soil of P. capsici-affected farms that continuously cropped pepper for 3, 6, and 9 years in Liaoning Province, China. The colony and cell morphology, physiological and biochemical characteristics, and 16S rDNA sequence of bacteria and actinomycetes were documented. In addition, colony and microscopic morphology of fungi and the rDNA-ITS sequence were analysed for classification. We observed that healthy and diseased peppers had the largest number of bacteria in the rhizosphere followed by actinomycetes and fungi. After infection, the number of bacteria and actinomycetes decreased with a corresponding increase in the number of fungi, leading to a reduction in the ratio of bacteria/fungi to actinomycetes/fungi. We identified 15 dominant bacterial strains, of which Bacillus represented the most abundant genus consisting of 7 strains followed by Flavobacterium and Staphylococcus. Furthermore, 15 of the 17 actinomycetes strains belonged to the genus Streptomyces. Among the six fungal strains, we found P. infestans, Fusarium, and Penicillium consisting of two strains each. This study elucidated the impact of pathogenic P. capsici on the composition of soil microbes over time and characterized several cultivatable dominant bacterial groups, which can provide a basis for practical intervention strategies to improve soil conditions for continuous cropping.

Keywords
continuous cropping
dominant microorganism
microbial community
pepper
Phytophthora capsica
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :4
  • Pages :431-445
  • Received Date : 2021-01-26
  • Revised Date : 2021-04-01
  • Accepted Date : 2021-04-22
  • DOI :https://doi.org/10.7235/HORT.20210039
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