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2024 Vol.42, Issue 5 Preview Page

Research Article

Selection of Differentially Expressed Genes Responding to High Temperatures in ‘Fuji’ Apple Tree Leaves by a Transcriptome Analysis

‘후지’ 사과나무 잎의 전사체 분석을 통한 고온에 특이적으로 반응하는 유전자 선발

Soon Young Ahn1
,
Zar Le Myint1
,
Seon Ae Kim2
,
Seung Heui Kim3
,
Hae Keun Yun1*
안 순영1
,
자 리민1
,
김 선애2
,
김 승희3
,
윤 해근1*
1Department of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Korea
2Apple Research Institute, National Institute of Horticultural & Herbal Science, RDA, Gunwi 39000, Korea
3Department of Fruits, Korea National University of Agriculture and Fisheries, Jeonju 54874, Korea
1영남대학교 원예생명과학과
2농촌진흥청 국립원예특작과학원 사과연구소
3한국농수산대학교 과수과
*Corresponding Author
31 October 2024. pp. 654-666
PDF XML Citation
Abstract

A transcriptome analysis was conducted to select differentially expressed genes (DEGs) from ‘Fuji’ apple tree leaves exposed to high temperatures (35°C) for 24 hours. A total of 34,158,132 and 41,475,948 paired-end reads for 25°C and 35°C samples of ‘Fuji’ apple tree leaves were obtained, respectively. The average read-length ranged from 90.0 to 90.2 bp and the analyzed reads were found to cover 82.8-83.1% of the raw data. DEGs were divided into 15, 11, and 30 main functional categories of molecular functions, cellular components, and biological processes with different degrees at level 3, respectively. The major up-regulated genes included a GDSL-like lipase/acylhydrolase superfamily protein, a cell wall protein precursor, MLP-like protein 423, heat shock protein 18.2, a HSP20-like chaperones superfamily protein, phytosulfokine receptor 1, a protein phosphatase 2C family protein, and microsomal glutathione s-transferase, while the major down-regulated genes included 3-ketoacyl-CoA synthase 19, a cysteine proteinase superfamily protein, the plant invertase/ pectin methylesterase inhibitor heat shock protein 70, ferretin 1, nitrate transporter 1.7, and heat shock protein 81-2 in apple leaves exposed to high temperatures. The selected DEGs were confirmed to be differentially expressed by high temperatures in a quantitative real time-PCR analysis with gene-specific primers. Transcriptome data can provide meaningful information about the discovery of specific gene and can elucidate the mechanism in apple trees responding to high temperatures, and selected DEGs can be used as genetic resources in the molecular breeding of apples tolerant to foreign stress sources such as high temperatures in the future.

Keywords
anthocyanin
global warming
heat response
real-time PCR
RNA-seq

전사체 분석을 이용하여 고온처리 한 사과나무 후지 잎에 차등발현하는 유전자를 확인하였다. 품질이 낮은 시퀀스를 제거한 후 25°C와 35°C 처리구에서 각각 총 34,158,132와 41,475,948의 transcripts를 확보하였다. 평균 길이는 25°C와 35°C 처리구에서 각각 90.0bp와 90.2bp이며, 평균 82.9%의 data가 분석에 활용되었다. 전사체 분석을 이용하여 24시간 동안 고온에 처리된 후지 사과나무 잎에서 차등발현(Differentially expressed genes)되는 유전자를 확인하였다. DEG는 3개의 주요 category인 biological processes, cellular components, molecular functions으로 나눠졌다. 고온에 노출된 사과나무 잎에서 발현이 유도되는 주요 유전자들은 GDSL-like lipase/acylhydrolase superfamily protein, cell wall protein precursor, MLP-like protein 423, heat shock protein 18.2, HSP20-like chaperone superfamily protein, phytosulfokin receptor 1, protein phosphatase 2C family protein, and microsomal glutathione s-transferase 등 이였으며, 반면 발현이 억제되는 유전자들은 3-ketoacyl-CoA synthase 19, cysteine proteinases superfamily protein, plant invertase/pectin methylesterase inhibitor, heat shock protein 70, ferretin 1, nitrate transporter 1.7, and heat shock protein 81-2 등 이였다. 모든 DEG는 gene-specific primer를 이용하여 quantitative real time-PCR로 검증하였다. 고온 처리 사과나무 잎의 전사체 분석 결과는 향후 연구를 위한 특이 유전자 발굴이나 고온 처리에 대한 사과나무 잎의 반응 기작을 설명하는데 있어 의미 있는 정보를 제공할 것이다.

키워드
안토시아닌
지구온난화
열 반응
real-time PCR
RNA-seq
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  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 42
  • No :5
  • Pages :654-666
  • Received Date : 2023-11-09
  • Revised Date : 2024-01-02
  • Accepted Date : 2024-01-02
  • DOI :https://doi.org/10.7235/HORT.20240050
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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