Reverse Transcription Loop-mediated Isothermal Amplification Assay for Detecting Peach Latent Mosaic Viroid in Peach Pollen

Hyo-Jeong Lee; Rae-Dong Jeong

doi:10.7235/HORT.20200075

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2020 Vol.38, Issue 6 Preview Page Next Page

Research Article

Reverse Transcription Loop-mediated Isothermal Amplification Assay for Detecting Peach Latent Mosaic Viroid in Peach Pollen

31 December 2020. pp. 830-839

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Abstract

Artificial pollination is used to increase the yield of many fruit crops. However, much of the pollen used for this process is imported from other countries, thereby increasing the risk of introducing plant pathogens, such as peach latent mosaic viroid (PLMVd). According to the Animal and Plant Quarantine Service in Korea, PLMVd is a quarantine pathogen that reduces both fruit yield and tree vigor. Therefore, the aim of the present study was to develop a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for detecting PLMVd in imported pollen. The optimum RT-LAMP conditions were incubation at 63°C for 60 min, and the assay was both highly specific and 10 times more sensitive than RT-PCR. The usefulness of the RT-LAMP assay was also confirmed by screening both imported pollen and leaf samples for PLMVd. Thus, the present study demonstrates the usefulness of RT-LAMP for the rapid screening of PLMVd in field conditions, peach certification programs, and quarantine inspections.

Keywords

diagnosis

quarantine

rapidity

sensitivity

specificity

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Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 38
No :6
Pages :830-839
Received Date : 2020-06-14
Revised Date : 2020-07-30
Accepted Date : 2020-08-26
DOI :https://doi.org/10.7235/HORT.20200075

Horticultural Science and TechnologyISSN:1226-8763(Print) 2465-8588(Online)원예과학기술지

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