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Effects of Light-emitting Diodes on In Vitro Growth of Virus-free Sweet Potato Plantlets

LED가 고구마 바이러스 무병묘의 기내 생장에 미치는 영향

Kyoung-Ran Yoo1
,
Seung-Yeob Lee1*
유 정란1
,
이 승엽1*
1Institute of Life Science and Natural Resources, Wonkwang University
1원광대학교 생명자원과학연구소
*교신저자. *Corresponding Author. 
31 August 2017. pp. 465-479
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Abstract

The in vitro growth of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets was investigated under different light sources: fluorescent lamp (control); red (660 nm), blue (460 nm), white light-emitting diodes (LED), and two mixtures of blue and red LED (R:B = 8:2, and 7:3). Single node explants (10 mm) of three cultivars (‘Matnami’, ‘Shincheonmi’, and ‘Yeonhwangmi’) were cultured on Murashige and Skoog medium supplemented with 0.2mg·L-1 6-benzyladenine for 4 weeks. Explants were exposed to 150 ± 5 μmol·m-2·s-1photosynthetic photon flux at a distance of 20 cm, constant temperature of 25°C, and under 16/8-h (day/ night) photoperiod. Using the same method, the in vitro growth of 10 cultivars under red LED was also compared. After 3 weeks, vine length was highest in plantlets cultured under red LED, and lowest in plantlets cultured under blue LED. Fresh and dry weights were also greatest in plantlets cultured under red LED. Compared to the control, vine thickness was significantly higher in plantlets grown under white LED and the 7:3 R:B LED mixture. Significant differences were observed among the 10 cultivars grown under red LED. ‘Matnami’, ‘Shincheonmi’, and ‘Shinhwangmi’ all had excellent vine lengths, and fresh and dry weights. Compared to the control, vine elongation of sweet potato plantlets was most effective under red LED, and culture duration was about 1 week shorter.

Keywords
dry weight
fresh weight
Ipomoea batatas
node culture
vine growth

고구마 바이러스 무병주의 기내 소식물체 생장에 미치는 LED의 영향을 조사하기 위하여, ‘맛나미’, ‘신천미’, ‘연황미’의 소식 물체에서 10mm 크기의 마디를 잘라 0.2mg·L-1 BA를 첨가한 MS배지에 배양하였다. 광질은 형광등을 대조구로 하여, 적색 (660nm), 청색(460nm), 백색, 적:청(8:2), 적:청(7:3) LED를 20cm 거리에서 150±5μmol·m-2·s-1 광합성유효광량자속(PPF)을 유지하였으며, 일장은 16/8(day/night)시간, 배양온도는 25°C로 조절하였다. LED 광질에 대한 품종간 반응은 적색 LED에서 같은 방법으로 10품종의 마디배양을 하여 소식물체 생장을 조사하였다. 배양 3주 후에 줄기신장은 적색 LED에서 형광등보다 유의하게 증가하였으며, 청색광에서 가장 낮았다. 줄기두께는 적:청(7:3)과 백색 LED에서 증가하였다. 생체중 및 건물중도 적색광에서 가장 높았다. 또한 적색 LED에서 신초생육은 유의한 품종간 차이를 보였다. 줄기길이, 생체중 및 건물중 등은 ‘신천 미’, ‘맛나미’, ‘신황미’ 등에서 컸다. 따라서 LED 광원을 이용한 고구마 마디배양은 적색 LED에서 3주간 배양하여 5cm 이상 키 운 소식물체를 토양이식하는 것이 대조구인 형광등보다 배양기간을1 주 이상 단축할 수 있었다 .

키워드
건물중
생체중
Ipomoea batatas
마디배양
줄기생장
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 35
  • No :4
  • Pages :465-479
  • DOI :https://doi.org/10.12972/kjhst.20170052
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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