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2021 Vol.39, Issue 4 Preview Page

Research Article

Identification and Characterization of MADS-box Genes Involved in Floral Organ Development in Chinese Chestnut (Castanea mollissima Blume)
Yang Liu12
,
Guosong Chen2
,
Yuerong Gao2
,
Kefeng Fang3
,
Qing Zhang4
,
Qingqin Cao2
,
Ling Qin2
,
Yu Xing2
,
Shuchai Su1*
1Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China
2Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China
3College of Landscape Architecture, Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing University of Agriculture, Beijing 102206, China
4College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China
*Corresponding Author
31 August 2021. pp. 482-496
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Abstract

Chinese chestnut (Castanea mollissima) is a woody plant species with important economic and ecological value and is widely cultivated for nut production. Due to its unique flowering structure, the identification of genes related to flower development is extremely important in Chinese chestnut, and MADS-box genes are directly involved in floral organ morphogenesis. In this study, we report a MADS-box gene family in Chinese chestnut. According to their gene structure and phylogenetic trees, 53 CmMADS proteins were identified and clustered into five groups in Chinese chestnut. The MIKCC group was further divided into 12 subgroups, and the BS subgroup was not found in Chinese chestnut. The classification and motif composition of Chinese chestnut MADS proteins were also analyzed. Additionally, we evaluated the expression of all the MADS-box genes in floral organs by quantitative real-time PCR. Overall, most of the type II genes had higher expression levels in the floral organs compared with leaves and cotyledons. These results provide valuable information for future studies of the regulation of MADS-box genes in Chinese chestnut during flower development.

Keywords
expression patterns
MADS proteins
motifs
phylogenetic analysis
reproductive organs
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :4
  • Pages :482-496
  • Received Date : 2021-01-17
  • Revised Date : 2021-02-10
  • Accepted Date : 2021-04-21
  • DOI :https://doi.org/10.7235/HORT.20210043
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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