Shoot Proliferation,  Rooting,  and Genetic Stability of Six Micropropagated Olive (Olea europaea L.) Genotypes

Abdou Abd Allatif; Ibrahim Hmmam

doi:10.7235/HORT.20220039

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2022 Vol.40, Issue 4 Preview Page

Research Article

Shoot Proliferation, Rooting, and Genetic Stability of Six Micropropagated Olive (Olea europaea L.) Genotypes

31 August 2022. pp. 432-445

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Abstract

This research aimed to explore the effect of zeatin (6-γ,γ-dimethylallyl-amino) concentration on the in vitro shoot proliferation of six olive (Olea europaea L.) cultivars, ‘Aggizi Shami,’ ‘Coratina,’ ‘Frantoio,’ ‘Manzanillo,’ ‘Picual,’ and ‘Toffahi.’ The six olive cultivars were reproduced via in vitro multiplication on Rugini olive medium (OM) with three zeatin concentrations (2, 4, and 6 mg L^-1) for six subcultures. The ‘Aggizi Shami’ and ‘Toffahi’ cultivars had the highest shoot numbers. Increasing zeatin concentration improved propagation. The best response was recorded for OM supplemented with zeatin at 6 mg·L^-1; this treatment resulted in high values for the numbers of shoots, leaves, and internodes. Then, the rooting ability of shoots was analyzed using two different rooting methods. The regenerated shoots were successfully rooted on ½OM supplemented with indole-3-butyric acid (IBA), with the ‘Coratina’ and ‘Picual’ cultivars having the highest rooting percentages. The response of shoots to the two rooting methods was genotype-dependent. Rooting in OM with IBA was more successful than dipping in IBA. Based on the shoot growth and rooting potential of the olive cultivars, ‘Coratina’ and ‘Picual’ were the most adaptable cultivars to the in vitro conditions, followed by ‘Frantoio’ and ‘Manzanillo.’ ‘Aggizi Shami’ and ‘Toffahi’ were recalcitrant cultivars to the in vitro conditions, as they exhibited low rooting potential. After six subcultures, the genetic stability of the six olive cultivars was determined. “Manzanillo” showed the highest genetic stability index (96%), while ‘Toffahi’ recorded the lowest value (85%), based on the start codon targeted (SCoT) data analysis.

Keywords

genetic stability

in vitro

multiplication

olive

rooting

SCoT

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Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Korean Journal of Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 40
No :4
Pages :432-445
Received Date : 2022-02-03
Revised Date : 2022-05-20
Accepted Date : 2022-06-22
DOI :https://doi.org/10.7235/HORT.20220039

Korean Journal of Horticultural Science and Technology 원예과학기술지 ISSN:1226-8763(Print) 2465-8588(Online)

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