Research Article

Horticultural Science and Technology. 31 December 2022. 654-662



  • Introduction

  • Materials and Methods

  •   Plant Materials and Growth Conditions

  •   Growth Characteristics

  •   Total Phenolics and Total Flavonoids

  •   Statistical Analysis

  • Results and Discussion

  • Conclusion


Salvia plebeia R. Br. is an annual or biennial plant included in the Lamiaceae family that is native to Korea, Japan, and China (Gu and Weng, 2001; Lim et al., 2007). Salvia plebeia R. Br. is known to improve allergy and airway inflammation, suppress asthma and rhinitis, and to have anti-inflammatory and hepatoprotective effects. For these reasons, it is in great demand as a medicinal crop (Yang et al., 2010; Costa et al., 2012; Talib et al., 2012; Choi et al., 2015; Liang et al., 2016; Jang et al., 2017). In addition, unlike most leafy vegetables for which only the shoots are used, both the shoots and roots of Salvia plebeian R. Br. can be used. It is commercially produced in Korea, and consumption is expected to increase in the future (Park et al., 2017).

The contents of bioactive compounds differ under different harvest periods, climates, and cultivation conditions (Zhao et al., 2008). Most medicinal plants used in Korea have non-uniform contents of bioactive compounds because they are harvested in the wild or grown in open fields and have irregular harvest periods (KREI, 2012; RDA, 2015). Therefore, the supply of many medicinal plants is intermittent with non-uniform quality of the bioactive components and yield. Therefore, it is necessary to develop a cultivation technology capable of controlling the environmental conditions for the stable supply and uniform quality of medicinal plants.

The closed-type plant production system (CPPS) achieves such optimal conditions by artificially controlling environmental conditions such as light levels, air temperatures, relative humidity levels, CO2 concentrations, and nutrient solutions (Takatsuji, 2008). CPPSs offer the advantages of year-round stability and the ability to produce crops of a high and uniform quality by providing the proper conditions. However, a disadvantage of the CPPS is that it requires a high initial investment and costly facilities (Lee and Cha, 2015). It is important to determine the economic feasibility of the commercial use of a CPPS. Recently, there have been many studies to improve the economic feasibility of CPPSs with the production of value crops (Heo et al., 2015; Heo and Baek, 2021).

Plants accumulate various bioactive compounds under stress conditions (Davies, 1995). These bioactive compounds prevent oxidative damage in the plants and prevent diseases caused by oxidative damage and cardiovascular diseases in humans (Stephen, 1999; Voutilainen et al., 2006). However, excessive stress conditions cause decreases in plant growth outcomes and yields (Boyer, 1982). Therefore, increasing the levels of bioactive compound without reducing the growth of crops can be a useful cultivation technique (Lee and Oh, 2015).

Temperature is one of the most important environmental conditions for plant growth. Growing plants under abnormal temperature conditions causes various physiological disorders and decreases their productivity (Lim et al., 2000; Hatfield and Prueger, 2015; Son et al., 2015). Sanghera et al. (2011) reported that excessively low temperatures cause stress during the cultivation period, leading to physiological disorders in plants due to the accumulation of osmolytes, the generation of reactive oxygen species, and degeneration of the cell membrane. However, other studies reported that plants under low-temperature stress show increased bioactive compound contents. Lee and Oh (2015) reported that short-term low-temperature conditions increased the phenolic compounds of kale. Other studies have reported that these short-term temperature stress can increase the bioactive compound contents of plants (Oh et al., 2009; Lee et al., 2012; Lee et al., 2014) However, research on bioactive compound contents in plants under low-temperature conditions is limited to only certain crops (Lee and Oh, 2015), meaning that there is a need for research applicable to growing various medicinal crops with CPPSs.

Therefore, this research was conducted to discover a short-term low-temperature treatment method that can be applied before harvest to increase the bioactive compound content of Salvia plebeia R. Br. When grown in a CPPS while maintaining its marketable quality.

Materials and Methods

Plant Materials and Growth Conditions

Seeds of Salvia plebeia R. Br. (Asia seed Co. Ltd., Seoul, Korea) were sown in 128-cell plug trays filled with urethane sponges (Hydroponic Sponge, Easyhydro Co. Ltd., Chuncheon, Korea) in CPPS (C1200H3, FC Poibe Co. Ltd., Seoul, Korea) at a temperature of 25 ± 1°C, a photoperiod of 12/12 h (light/dark), and a photosynthetic photon flux density (PPFD) of 180 ± 10 µmol·m-2·s-1 using RGB LEDs (red:green:blue = 7:1:2, ES LEDs Co. Ltd., Seoul, Korea). Seedlings at 45 days were transplanted into a deep floating technique system with recycling Hoagland nutrient solution at pH 6.5 and EC 1.5 dS·m-1 in a CPPS (Hoagland and Anon, 1950). The plants were cultured for 36 days under growth conditions identical to the germination conditions. The light intensity level was set using a photometer (HD2101.1, Delta OHM Co. Ltd., Padova, Italy) and light spectral distributions were measured using a spectroradiometer (ILT950, International Light Co. Ltd., MA, USA). The following low-temperature treatments were used consistently: 15 ± 1°C for 1, 3, 5, 10, or 15 days before harvest (Fig. 1).
Fig. 1.

Experiment schedule for the low-temperature treatments. (Low-temperature treatments were 15 ± 1℃ from 1, 3, 5, 10, and 15 days before harvest; D1, D3, D5, D10, and D15, respectively).

Growth Characteristics

After the low-temperature treatment, the leaf length, leaf width, fresh and dry weights of the shoots and roots, the number of leaves, the leaf area, the SPAD value, and the chlorophyll fluorescence were measured. The leaf area was measured using a leaf area meter (LI-3100, LI-COR Inc., Nebraska, USA). Fresh weights of shoots and roots were obtained using an electronic balance (EW 220-3NM, Kern and Sohn GmbH., Balingen, Germany), and the dry weights of the shoots and roots were measured after drying these components in an oven (Venticell-222, MMM Medcenter Einrichtungen GmbH., Munich, Germany) at 70°C for 72 h. The SPAD value was measured using a portable chlorophyll meter (SPAD-502, Konica Minolta Inc., Tokyo, Japan). The chlorophyll fluorescence was measured on third leaf from the top using a portable chlorophyll fluorescence meter (FluorPen FP100, Photon Systems Instruments Brno, Czech Republic). The specific leaf area (SLA) and leaf shape index (LSI) were calculated as per the following formulae:

SLA (mg·cm-2) = dry weight of leaf (mg) / total leaf area (cm2)

Leaf shape index = leaf length / leaf area

The photosynthetic rate was measured using a portable photosynthesis system (CIRAS-3, PP Systems International Inc., MA, USA) on a completely unfolded fifth leaf from the apex. The measurement conditions were controlled as follows: air flow rate 150 mL·min-1, leaf area 4.5 mm2, leaf temperature 25°C, CO2 concentration 500 µmol·mol-1 and a PPFD of 180 µmol·m-2·s-1.

Total Phenolics and Total Flavonoids

A 1 g leaf sample was immediately frozen in liquid nitrogen and then finely ground. To extract the phenolic compounds, the sample was mixed with 5 mL of 80% methanol. The mixture was shaken for 24 h at room temperature and then centrifuged at 10,509 ×g for 10 min. After centrifugation, the supernatant was used to determine the total phenolic concentration. The total phenolic concentrations in Salvia plebeia R. Br. samples were determined using the Folin–Ciocalteu reagent method of Singleton and Rossi (1965). Approximately 200 µL of extract was mixed with 300 µL distilled water and 250 µL 2N Folin–Ciocalteu reagent (Sigma-Aldrich Co., St. Louis, MO, USA). The mixture was then combined with 1.25 mL of 20% Na2CO3, vortexed for 5 s, and incubated at room temperature for 20 min. The absorbance of the supernatant was measured with a spectrophotometer (Libra S22, Biochrom Ltd., Cambridge, UK) at 735 nm to determine the total phenolic concentration, which was expressed as g gallic acid equivalent (GAE) per mg of FW.

The extraction method for the total flavonoid concentration of the sample was identical to that used for the total phenolic concentration. The total flavonoid concentration of the leaf was determined using a modified form of the method of Kumaran and Karunakaran (2007). A volume of 900 µL of 80% methanol and 1 mL of 2% AlCl3 were added to 100 µL of the extract. After vortexing for 2 s, the mixture was reacted at room temperature for 30 min. The absorbance of the samples was measured with a spectrophotometer at 415 nm to determine the total flavonoid concentration, which was expressed as g quercetin equivalent (QE) per mg of FW.
Fig. 2.

Growth of Salvia plebeia R. Br. affected by low-temperature treatments 36 days after transplanting.

Statistical Analysis

The experiments were repeated three times with ten plants per repetition for each treatment, each in a randomized complete block design. After selecting plants of a uniform size, nine plants per treatment were used to determine the plant growth parameters. The statistical analysis was carried out using the Statistical Analysis System program (SAS 9.1, SAS Institute Inc., Cary, NC, USA). Experimental results were subjected to analysis of variance (ANOVA) and Duncan’s multiple range tests. The SigmaPlot program was used for graphing (SigmaPlot 12.0, Systat Software Inc., San Jose, CA, USA).

Results and Discussion

The growth parameters of Salvia plebeia R. Br. 36 days after transplanting are shown in Table 1. The fresh weights of the roots did not differ significantly among the low-temperature treatments. However, the leaf length, leaf width, and fresh and dry weights of the shoots were decreased in D5, D10, and D15. Sanghera et al. (2011) reported that excessively low temperatures (0–15°C) cause physiological disorders in plants. Earlier studies reported that low temperatures decreased the growth of hot pepper and kale (Park et al., 2014; Hwang et al., 2017). In our study, the growth of Salvia plebeia R. Br. decreased when exposed to a low temperature for more than five days.

Table 1.

Growth Characteristics of Salvia plebeia R. Br. after Low-Temperature Treatments 36 days after Transplanting

Treatmentz Leaf length
Leaf width
Fresh weight (g/plant) Dry weight (g/plant)
Shoot Root Shoot Root
Control 12.2 ay 6.3 b 55.8 a 22.5 a 5.6 a 1.3 a
D1 12.6 a 7.1 a 52.6 a 22.9 a 5.1 a 1.4 a
D3 11.8 ab 6.6 ab 50.5 ab 22.1 a 5.2 a 1.3 a
D5 10.5 bc 6.4 ab 41.4 bc 22.0 a 4.1 b 1.2 a
D10 9.1 cd 6.2 b 36.8 c 18.8 a 4.2 b 1.3 a
D15 7.9 d 6.2 b 35.2 c 19.0 a 3.7 b 0.9 b

zThe low-temperature treatments used were 15 ± 1°C from 1, 3, 5, 10, and 15 days before harvest (D1, D3, D5, D10, and D15, respectively).

yMean separation within columns by Tukey’s multiple range test at p < 0.05 (n = 6).

The numbers of leaves, leaf area, SLA, and LSI tended to decrease as the duration of the low-temperature treatment was increased (Fig. 3). Williams and Black (1993) reported that the leaf area of Pennisetum setaceum decreased as it was exposed to a low-temperature condition. In this study, as the duration of the low temperature increased, the leaf area decreased, and it appears that the decrease in the number of leaves affected the decrease in the leaf area. The SLA is related to the thickness of the leaf, and a high SLA means a thicker leaf. Rodríguez et al. (2015) reported that cabbage and kale had thicker leaves when exposed low temperatures. In the current study, in the D10 and D15 treatments, which had long periods of exposure to a low temperature, the SLAs were lower (0.19 and 0.21, respectively). These findings suggest that the decrease of the dry weight of the shoots at the low temperature affected the SLA.
Fig. 3.

Number of leaves (A), leaf area (B), specific leaf area (C), and leaf shape index (D) of Salvia plebeia R. Br. affected by low-temperature treatments 36 days after transplanting. The low-temperature treatments were 15 ± 1°C from 1, 3, 5, 10, and 15 days before harvest (D1, D3, D5, D10, and D15, respectively). Vertical bars represent the standard deviation of the mean (n = 6). Different letters in the same column indicate significant differences based on Duncan’s multiple range test (p < 0.05).

The photosynthesis rates of Salvia plebeia R. Br. were highest in D1 and the SPADs were highest in D10 (Fig. 4A). Generally, it is known that the photosynthesis rate increases as the temperature increases within a range that does not impair the growth of plants. Jo et al. (2016) reported that the photosynthesis rate of paprika seedlings decreased under low-temperature conditions. In our results, the photosynthesis rate of Salvia plebeia R. Br. decreased as the period of exposure to a low temperature increased. Lakshmanan et al. (2007) reported that the accumulated chlorophyll per unit area increased as the leaf area decreased, and it appears that the SPAD value in our results had a similar tendency.

Chlorophyll fluorescence (Fv/Fm) decreased in the D3 treatment and then increased as the period of exposure to the low temperature increased (Fig. 4C). It is known that chlorophyll fluorescence decreases when a plant is exposed to biotic or abiotic stress (Kycko et al., 2018). Under optimal conditions, the Fv/Fm ratio of plants is known to be about 0.83 (Maxwell and Johnson, 2000); if the Fv/Fm ratio decreases to less than 0.83, the plant is under stress. In our results, the Fv/Fm of Salvia plebeia R. Br. decreased for up to three days at the low temperature and increased again after five days. It appears that the plant adapted to the low temperature after five days and that the defense mechanism of the plant was activated.
Fig. 4.

Photosynthetic rate (A), SPAD value (B), and chlorophyll fluorescence (C) of Salvia plebeia R. Br. affected by low-temperature treatments 36 days after transplanting. The low-temperature treatments were 15 ± 1°C from 1, 3, 5, 10, and 15 days before harvest (D1, D3, D5, D10, and D15, respectively). Vertical bars represent the standard deviation of the mean (n = 6). Different letters in the same column indicate significant differences based on Duncan’s multiple range test (p < 0.05).

The total phenolics and flavonoids of Salvia plebeia R. Br. exposed to low temperatures are shown in Fig. 5. Under environmental stress conditions, the plants accumulated these bioactive compound contents (Dixon and Paiva, 1995). Early studies reported that many crops show increased bioactive compound contents when exposed to low-temperature conditions (Rivero et al., 2001; Oh et al., 2009; Neugart et al., 2012). In this study, the total phenolic and flavonoid concentrations of Salvia plebeia R. Br. were highest in the D3 and D10 treatments (Fig. 5A and 5C); however, the total contents in the plants were highest in D3 (Fig. 5B and 5D). The concentrations of total phenolics and total flavonoids tended to decrease with the duration of exposure to the low temperatures, and in the D3 treatment, there was relatively little reduction in growth such that the D3 plants had high total phenolic and flavonoid contents.
Fig. 5.

Contents of bioactive compounds in Salvia plebeia R. Br. affected by low-temperature treatments 36 days after transplanting. The low-temperature treatments were 15 ± 1°C from 1, 3, 5, 10, and 15 days before harvest (D1, D3, D5, D10, and D15, respectively). Vertical bars represent the standard deviation of the mean (n = 6). Different letters in the same column indicate significant differences based on Duncan’s multiple range test (p < 0.05).


When considering the economic prospects of CPPSs, it is important to produce plants with high added value, such as medicinal plants. In this study, Salvia plebeia R. Br. was produced in a CPPS hydroponic system. Low temperatures increased the content of the bioactive compounds of Salvia plebeia R. Br.; however, longer periods of a low-temperature treatment decreased both the growth of Salvia plebeia R. Br. and the content of its bioactive compounds. We conclude that exposure to low temperatures three days before harvesting the plants offers advantages for producing high-quality Salvia plebeia R. Br. with marketable value owing to the increased bioactive contents without decreased growth.


Boyer JS (1982) Plant productivity and environment. Science 218:443-448. doi:10.1126/science.218.4571.443 10.1126/science.218.4571.44317808529
Choi BO, Yin, HH, Fang CZ, Ha HO, Kim SJ, Jeong SJ, Jang SI (2015) Synergistic anti-inflammatory effect of rosmarinic acid and luteolin in lipopolysaccharide-stimulated RAW264.7 macrophage cells. Korean J Food Sci Technol 47:119-125. doi:10.9721/KJFST.2015.47.1.119 10.9721/KJFST.2015.47.1.119
Costa RS, Carneiro TCB, Cerqueira-Lima AT, Queiroz NV, Alcântara-Neves NM, Pontes-de-Carvalho LC, Velozo Eda S, Oliveira EJ, Figueiredo CA (2012) Ocimum gratissimum Linn. and rosmarinic acid, attenuate eosinophilic airway inflammation in an experimental model of respiratory allergy to Blomia tropicalis. Int Immunopharmacol 13:126-134. doi:10.1016/j.intimp.2012.03.012 10.1016/j.intimp.2012.03.01222465960
Davies KJA (1995) Oxidative stress: The paradox of aerobic life. Biochem Soc Symp 61:1-32. doi:10.1042/bss0610001 10.1042/bss06100018660387
Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085-1097. doi:10.2307/3870059 10.2307/387005912242399PMC160915
Gu L, Weng X (2001) Antioxidant activity and component of Salvia plebeia R. Br.- a Chinese herb. Food Chem 73:299-305. doi:10.1016/S0308-8146(00)00300-9 10.1016/S0308-8146(00)00300-9
Hatfield JL, Prueger JH (2015) Temperature extremes: Effect on plant growth and development. Weather Clim Extrem 10:4-10. doi:10.1016/j.wace.2015.08.001 10.1016/j.wace.2015.08.001
Heo JW, Baek JH (2021) Effects of light-quality control on the plant growth in a plant factory system of artificial light type. Korean J Environ Agric 40:270-278. doi:10.5338/KJEA.2021.40.4.31 10.5338/KJEA.2021.40.4.31
Heo JW, Kim HH, Lee KJ, Yoon JB, Lee JK, Huh YS, Lee KY (2015) Effect of supplementary radiation on growth of greenhouse-grown kales. Korean J Environ Agric 34:38-45. doi:10.5338/KJEA.2015.34.1.02 10.5338/KJEA.2015.34.1.02
Hoagland DR, Aron DI (1950) The water-culture method for growing plants without plant. 3rd ed. Univ. Calif. Agric. Exp. Stat. Circular 347, CA, USA
Hwang SJ, Chun JH, Kim SJ (2017) Effect of cold stress on carotenoids in kale leaves (Brassica oleracea). Korean J Environ Agric 36:106-112. doi:10.5338/KJEA.2017.36.2.19 10.5338/KJEA.2017.36.2.19
Jang TY, Jung AY, Kyung TS, Kim DY, Hwang JH, Kim YH (2017) Anti-allergic effect of luteolin in mice with allergic asthma and rhinitis. Cent Eur J Immunol 42:24-29. doi:10.5114/ceji.2017.67315 10.5114/ceji.2017.6731528680328PMC5470611
Jo YH, Kim CS, Kim JM, Ku YG, Kim HC (2016) Qualities and early growth responses of paprika seedlings grown in high and low temperatures. Korean J Hortic Sci Technol 34:719-726. doi:10.12972/kjhst.20160075 10.12972/kjhst.20160075
Korea Rural Economic Institute (KREI) (2012) Medicinal plant supply and demand trend and policy issue.
Kumaran A, Karunakaran J (2007) In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Food Sci Technol 40:344-352. doi:10.1016/j.lwt.2005.09.011 10.1016/j.lwt.2005.09.011
Kycko M, Zagajewski B, Lavender S, Romanowska E, Zwijacz-Kozica M (2018) The impact of tourist traffic on the condition and cell structures of Alpine swards. Remote Sens 10:220-241. doi:10.3390/rs10020220 10.3390/rs10020220
Lakshmanan GMA, Jaleel CA, Gomathinayagam M, Panneerselvam R (2007) Changes in antioxidant potential and sink-organ dry matter with pigment accumulation induced by hexaconazole in Plectranthus forskholii Briq. C R Biol 330:814-820. doi:10.1016/j.crvi.2007.08.008 10.1016/j.crvi.2007.08.00817923375
Lee JE, Cha WC (2015) An analysis of the professional's cognition regarding the plant factory feasibility. J Digital Converg 13:89-97. doi:10.14400/JDC.2015.13.12.89 10.14400/JDC.2015.13.12.89
Lee JH, Oh MM (2015) Short-term low temperature increase phenolic antioxidant levels if kale. Hortic Environ Biote 56:588-596. doi:10.1007/s13580-015-0056-7 10.1007/s13580-015-0056-7
Lee MJ, Lim SY, Kim JK, Oh MM (2012) Heat shock treatments induce the accumulation of phytochemicals in kale sprouts. Korean J Hortic Sci Technol 30:509-518. doi:10.7235/hort.2012.12094 10.7235/hort.2012.12094
Lee MJ, Son JE, Oh MM (2014) Growth and phenolic compounds of Lactuca sativa L. grown in a closed-type plant production system with UV-A,-B, or -C lamp. J Sci Food Agric 94:197-204. doi:10.1002/jsfa.6227 10.1002/jsfa.622723670268
Liang Z, Nie H, Xu Y, Peng J, Zeng Y, Wei Y, Wen X, Qiu J, Zhong W, et al. (2016) Therapeutic effects of rosmarinic acid on airway responses in a murine model of asthma. Int Immunopharmacol 41:90-97. doi:10.1016/j.intimp.2016.10.010 10.1016/j.intimp.2016.10.01027825045
Lim JA, Yun BW, Baek SH (2007) Antioxidative activity and nitrite scavenging ability of methanol extract from Salvia plebeia R. Br. Korean J Medicinal Crop Sci 15:183-188
Lim MS, Shin KY, Woo JG, Kwon YS, Jang SW, Kim WB, Lee JN, Lee JT, Kwon HJ, et al. (2000) Vegetable cultivation technique in highland area. Kwahakwonyae press, Seoul, Korea, pp 52-26
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence: a practical guide. J Exp Bot 51:68-668. doi:10.1093/jexbot/51.345.659 10.1093/jexbot/51.345.65910938857
Neugart S, Kläring HP, Zietz M, Schreiner M, Rohn S, Kroh LW, Krumbein A (2012) The effect of temperature and radiation on flavonol aglycones and flavonol glycosides of kale (Brassica oleracea var. sabellica). Food Chem 133:1456-1465. doi:10.1016/j.foodchem.2012.02.034 10.1016/j.foodchem.2012.02.034
Oh MM, Carey EE, Rajashekar CB (2009) Environmental stresses induce health-promoting phytochemicals in lettuce. Plant Physiol Biochem 47:578-583. doi:10.1016/j.plaphy.2009.02.008 10.1016/j.plaphy.2009.02.00819297184
Park EG, Heo Y, Son BG, Choi YW, Lee YJ, Park YH, Suh JM, Cho JH, Hong CO, et al. (2014) The influence of abnormally low temperatures on growth and yield of hot Pepper (Capsicum annum L.). J Environ Sci Intl 23:781-786. doi:10.5322/JESI.2014.5.781 10.5322/JESI.2014.5.781
Park H, Yu YJ, Choi EY (2017) Effects of fluorescent light and light-emitting diodes on Leaf morphology, growth and antioxidant capacity of Salvia plebeia. Protected Hortic Plant Fac 26:208-214. doi:10.12791/KSBEC.2017.26.3.208 10.12791/KSBEC.2017.26.3.208
RDA (Rural Development Administration) (2015) Astragalus membranaceus Bunge farming skill guide. Wanju, Korea, pp 128-131
Rivero RM, Ruiz JM, Garcıa PC, Lopez-Lefebre LR, Sánchez E, Romero L (2001) Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Sci 160:315-321. doi:10.1016/S0168-9452(00)00395-2 10.1016/S0168-9452(00)00395-211164603
Rodríguez VM, Soengas P, Alonso-Villaverde V, Sotelo T, Cartea ME, Velasco P (2015) Effect of temperature stress on the early vegetative development of Brassica oleracea L. BMC Plant Biol 15:145-153. doi:10.1186/s12870-015-0535-0 10.1186/s12870-015-0535-026077340PMC4467057
Sanghera GS, Wani SH, Hussain W, Singh NB (2011) Engineering cold stress tolerance in crop plants. Current Genomics 12:30-43. doi:10.2174/138920211794520178 10.2174/13892021179452017821886453PMC3129041
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomoly-phosphotungstic acid reagents. Am J Enol Viticult 16:144-157
Son IC, Moon KH, Song EY, Oh SJ, Seo HH, Moon YE, Yang JY (2015) Effects of differentiated temperature based on growing season temperature on growth and physiological response in chinese cabbage 'Chunkwang'. Korean J Agri Forest Meteorology 17:254-260. doi:10.5532/KJAFM.2015.17.3.254 10.5532/KJAFM.2015.17.3.254
Stephen BK (1999) β-carotene, carotenoids and the prevention of coronary heart disease. J Nutr 129:5-8. doi:10.1093/jn/129.1.5 10.1093/jn/129.1.59915867
Takatsuji M (2008) Definition and meaning of the plant factory. World Science Publishment, Seoul, Korea, pp 8-13
Talib WH, Zarga MH, Mahasneh AM (2012) Antiproliferative, antimicrobial and apoptosis inducing effects of compounds isolated from Inula viscosa. Molecules 17:3291-3303. doi:10.3390/molecules17033291 10.3390/molecules1703329122418930PMC6268972
Voutilainen S, Nurmi T, Mursu J, Rissanen TH (2006) Carotenoids and cardiovascular health. The American J Clin Nutr 83:1265-1271. doi:10.1093/ajcn/83.6.1265 10.1093/ajcn/83.6.126516762935
Williams DG, Black RA (1993) Phenotypic variation in contrasting temperature environments: Growth and photosynthesis in Pennisetum setaceum from different altitudes on Hawaii. Funct Ecol 7:623-33. doi:10.2307/2390140 10.2307/2390140
Yang JM, Hung CM, Fu CN, Lee JC, Huang CH, Yang MH, Lin CL, Kao JY, Way TD (2010) Hispidulin sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by AMPK activation leading to Mcl-1 block in translation. J Agri Food Chem 58:10020-10026. doi:10.1021/jf102304g 10.1021/jf102304g20734985
Zhao Y, Li Z, Zhou X, Cai Z, Gong X, Zhou C (2008) Quality evaluation of Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography with photodiode-array detection. J Pharm Biomed Anal 48:1230-1236. doi:10.1016/j.jpba.2008.08.035 10.1016/j.jpba.2008.08.03518930617
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