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2025 Vol.43, Issue 6 Preview Page

Research Article

Physiological and Biochemical Responses of Allium mongolicum Regel Seeds to Drying Treatments at Various Temperatures
Zhenyu Cao1
,
Pengchao Wei1
,
Fenglan Zhang1
,
Xiaoyan Zhang1
,
Zhongren Yang1*
1School of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010000, China
*Corresponding Author
31 December 2025. pp. 705-721
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Abstract

This study investigated the mechanism and feasibility of drying Allium mongolicum Regel seeds to different moisture content (MC) levels for storage. The seeds were dehydrated to five MC levels ranging from 1.5% to 6.0% and stored at −20, 20, 35, and 50°C for up to 420 days. Seed vigor and various physiological and biochemical indicators were subsequently evaluated. Both germination and vigor were significantly influenced by the storage temperature and MC. Seeds dried to a moderate MC level (4.5–6.0%) and stored at 20–35°C showed higher germination percentages compared to non-dehydrated or over-dehydrated seeds. Moreover, extreme storage temperatures (−20°C or 50°C) suppressed germination vigor and antioxidant enzyme activity while increasing the accumulation of harmful metabolites, thereby accelerating seed deterioration. Germination percentage was positively correlated with soluble protein and proline contents, as well as polyphenol oxidase (PPO) and catalase (CAT) activities; it was negatively correlated with glutathione peroxidase (GPX) activity, the malondialdehyde (MDA) content, and the superoxide anion (O2‒) production rate. These correlations were especially evident in seeds with 4.5–6.0% MC. Therefore, drying seeds to 4.5–6.0% MC and storing them at 20–35°C for 180–360 days is recommended for the optimal preservation of A. mongolicum germplasm resources.

Keywords
correlation analysis
dehydration level
germination performance
osmotic regulation
storage conditions
References
1

Afzal I, Khalid E, Maqsood S, Basra A, Afzal A, Mahmood K (2019) Full length article maintaining seed quality of maize and wheat through dry chain technology in pakistan. Intl J Agric Biol 22:1363-1368. https://doi.org/10.17957/IJAB/15.1209

10.17957/IJAB/15.1209
2

Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual review of plant biology 55:373-399. https://doi.org/10.1146/annurev.arplant.55.031903.141701

10.1146/annurev.arplant.55.031903.141701
3

Baker J, Van dennSteele C, Dure L (1988) Sequence and characterization of 6 Lea proteins and their genes from cotton. Plant Molecular Biology 11:277-291. https://doi.org/10.1007/BF00027385

10.1007/BF00027385
4

Bakhtavar MA, Afzal I (2020) Preserving wheat grain quality and preventing aflatoxin accumulation during storage without pesticides using dry chain technology. Environ Sci Pollut Res 27:42064-42071. https://doi.org/10.1007/s11356-020-10212-5

10.1007/s11356-020-10212-5
5

Ballesteros D, Fanega-Sleziak N, Davies RM (2020) Cryopreservation of seeds and seed embryos in Orthodox-, Intermediate-, and recalcitrant-seeded species. Methods Mol Biol 663-682. https://doi.org/10.1007/978-1-0716-0783-1_36

10.1007/978-1-0716-0783-1_36
6

Ballesteros D, Walters C (2011) Detailed characterization of mechanical properties and molecular mobility within dry seed glasses: relevance to the physiology of dry biological systems. The Plant J 68:607-619. https://doi.org/10.1111/j.1365-313X.2011.04711.x

10.1111/j.1365-313X.2011.04711.x
7

Bewley JD, Marcus A (1990) Gene expression in seed development and germination. Prog Nucleic Acid Res Mol Biol 38:165-193. https://doi.org/10.1016/S0079-6603(08)60711-4

10.1016/S0079-6603(08)60711-4
8

Blackman SA, Wettlaufer SH, Obendorf RL, Leopold AC (1991) Maturation proteins associated with desiccation tolerance in soybean. Plant Physiol 96:868-874. https://doi.org/10.1104/pp.96.3.868

10.1104/pp.96.3.86816668267PMC1080857
9

Buitink J, Olivier L (2004) Glass formation in plant anhydrobiotes: survival in the dry state. Cryobiology 48:215-228. https://doi.org/10.1016/j.cryobiol.2004.02.011

10.1016/j.cryobiol.2004.02.011
10

Choi Y, Jeong CW, Ohr H, Song SK, Choi YD, Lee JS (2009) Developmental and environmental regulation of soybean SE60 gene expression during embryogenesis and germination. Planta 230:959-971. https://doi.org/10.1007/s00425-009-0999-y

10.1007/s00425-009-0999-y
11

Ellis RH, Hong TD (2006) Temperature sensitivity of the low-moisture-content limit to negative seed longevity–moisture content relationships in hermetic storage. Ann Bot 97:785-791. https://doi.org/10.1093/aob/mcl035

10.1093/aob/mcl03516495314PMC2803418
12

Endoh K, Itahana N, Matsushita M, Yamada H, Ubukata M (2021) Seed production and storage for endangered Morus boninensis using an ex‐situ living collection. Plant Biol 23:956-961. https://doi.org/10.1111/plb.13325

10.1111/plb.13325
13

Europe PMC (2016) Improvement of measurement method for superoxide anion radical in plant. Europepmcorg

14

Gao Y (2017) International seed testing association. China Standardization 01:150-155

15

Guo S, Wang Y, Wang W (2012) Effects of priming treatments on germination and biochemical characteristics of Pinus bungeana seeds. For Stud China 14:200-204. https://doi.org/10.1007/s11632-012-0302-3

10.1007/s11632-012-0302-3
16

Han B, Fernandez V, Pritchard HW, Colville L (2021) Gaseous environment modulates volatile emission and viability loss during seed artificial ageing. Planta 253. https://doi.org/10.1007/s00425-021-03620-5

10.1007/s00425-021-03620-533864524PMC8053187
17

Huo PH, Li JF, Liu JX, Zhang SQ, Shi SL (2015) Cross adaptation of medicago sativa seedlings germinated from ultra-dried seeds to saline and alkaline stresses. Int J Agric Biol 17:860-868. https://doi.org/10.17957/IJAB/15.0015

10.17957/IJAB/15.0015
18

Jin Y, Zhang T, Liu B, Zheng C, Huo H, Zhang J (2022) Comparative and phylogenetic analysis of the complete chloroplast genome sequences of Allium mongolicum. Sci Rep 12:21676. https://doi.org/10.1038/s41598-022-26354-0

10.1038/s41598-022-26354-036522492PMC9755143
19

Keeble F, Rayner MC (2021) Practical Plant Physiology. Read Books Ltd

20

Kermode AR (1990) Regulatory mechanisms involved in the transition from seed development to germination. Crit Rev Plant Sci 9:155-195. https://doi.org/10.1080/07352689009382286

10.1080/07352689009382286
21

Møller IM (2001) Plantmitochondria andoxidativestress: Electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annu Rev Plant Physiol Plant Mol Biol 52:561-591. https://doi.org/10.1146/annurev.arplant.52.1.561

10.1146/annurev.arplant.52.1.561
22

Rajjou L, Debeaujon I (2008) Seed longevity: Survival and maintenance of high germination ability of dry seeds. C R Biol 331:796-805. https://doi.org/10.1016/j.crvi.2008.07.021

10.1016/j.crvi.2008.07.021
23

Song X, Yang Z, Zhang D, Zhang X, Zhang F, Liu J, Yu C (2023) Proteomic analysis of the effect of accelerated ageing on Allium mongolicum seeds. Horticulturae 9:1155-1155. https://doi.org/10.3390/horticulturae9101155

10.3390/horticulturae9101155
24

Telewski FW, Jan Rijn Z (2002) The 120-yr period for Dr. Beal's seed viability experiment. Am J Bot 89:1285-1288. https://doi.org/10.3732/ajb.89.8.1285

10.3732/ajb.89.8.1285
25

Visscher AM, Seal CE, Newton RJ, Frances AL, Pritchard HW (2016) Dry seeds and environmental extremes: consequences for seed lifespan and germination. Funct Plant Biol 43:656. https://doi.org/10.1071/FP15275

10.1071/FP15275
26

Walters C (2015) Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss. Planta 242:397-406. https://doi.org/10.1007/s00425-015-2312-6

10.1007/s00425-015-2312-6
27

Walters C, Ballesteros D, Vertucci VA (2010) Structural mechanics of seed deterioration: Standing the test of time. Plant Sci 179:565-573. https://doi.org/10.1016/j.plantsci.2010.06.016

10.1016/j.plantsci.2010.06.016
28

Xie C, Liu T, Guo S, Peng J, Li Z (2021) Effects of ultra-dry storage on seed germination and seedling growth of Handeliondendron bodinieri. Silva Fenn 55:10509. https://doi.org/10.14214/sf.10509

10.14214/sf.10509
29

Yan M (2016) The preliminary study on the optimum moisture content of ultra-dry storage and its related chemicals in seeds from six crop species. Plant Genet Res 15:506-514. https://doi.org/10.1017/S1479262116000216

10.1017/S1479262116000216
30

Zhang J, Wang H, Liao S, Cui K (2018) Appropriate ultra-low seed moisture content stabilizes the seed longevity of Calocedrus macrolepis, associated with changes in endogenous hormones, antioxidant enzymes, soluble sugars and unsaturated fatty acids. New Forest 50:455-468. https://doi.org/10.1007/s11056-018-9670-4

10.1007/s11056-018-9670-4
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :6
  • Pages :705-721
  • Received Date : 2025-07-03
  • Revised Date : 2025-06-18
  • Accepted Date : 2025-08-25
  • DOI :https://doi.org/10.7235/HORT.20250062
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