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2016 Vol.34, Issue 2 Preview Page
Development of an Efficient Screening Method for Resistance of Chili Pepper Plants to Meloidogyne incognita

뿌리혹선충 Meloidogyne incognita에 대한 저항성 고추를 선발하기 위한 효율적인 검정법 확립

Sung Min Hwang1
,
Kyoung Soo Jang1
,
Yong Ho Choi1
,
Gyung Ja Choi1*
황 성민1
,
장 경수1
,
최 용호1
,
최 경자1*
1Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology
1한국화학연구원 친환경신물질연구센터
*교신저자. *Corresponding Author. 
30 April 2016. pp. 282-293
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Abstract

Root-knot nematodes (Meloidogyne spp.) are major plant pathogens that cause reductions in yield and quality of several solanaceous crops, including pepper (Capsicum spp.). These losses can be averted through planting of resistant cultivars. Plants are defined as resistant when they suppress nematode reproduction. In this study, the resistance degrees of 102 commercial cultivars of chili pepper (Capsicum annuum) to a root-knot nematode, Meloidogyne incognita, were evaluated by comparing the number of egg masses on their roots to those of ‘PR huimangchan’, a highly susceptible cultivar that exhibited the most egg masses of the chili pepper cultivars evaluated. Among these cultivars, forty-four (43.1%) showed resistance to M. incognita and eighteen (17.6%) were moderately resistant. The other cultivars (39.3%) were determined to be susceptible. For further study, six chili pepper cultivars (i.e., Gangryeokjosenggeon, Shinsegae, Muhanjilju, PR Bulrocho, PR Huimangchan, and Jjang) with different levels of resistance to the nematode were selected. Changes in resistance of the six cultivars under several conditions, such as inoculum concentration, plant growth stage, and cultivation period after transplanting were investigated. We found that an efficient screening method for resistance of chili pepper to M. incognita is to transplant the chili pepper seedlings 7 days before inoculation, to inoculate 28-day-old plants with M. incognita by loading 5,000 eggs per plant into the pot of soil, to cultivate the plants in a greenhouse (25 ± 5°C) for 45-60 days, to measure the number of egg masses on roots of the seedlings, and then to determine the resistance response of the plants by comparing the number of egg masses on the roots with a reference-susceptible cultivar ‘PR huimangchan’.

Keywords
breeding
egg mass
reproduction
resistant cultivar
root-knot nematode

뿌리혹선충(Meloidogyne spp.)은 고추(Capsicum spp.)를 포함한 여러 가지과 작물에서 경제적인 손실을 일으키는 주요 식 물 병원균 중 하나이다. 이러한 뿌리혹선충에 의한 작물의 피해는 친환경적인 방제법인 저항성 품종의 재배로 줄일 수 있 다. 선충병에서는 식물에서 선충의 후대생산이 억제되었을 때 그 식물을 저항성으로 간주한다. 시판 중인 고추(Capsicum annuum) 품종 102개의 뿌리혹선충(Meloidogyne incognita)에 대한 저항성 정도를 감수성 대조 품종인 ‘PR 희망찬’에 형성된 난낭의 수와 비교하여 평가하였다. 실험한 고추 품종 중 44개 품종(43.1%)은 저항성을 그리고 18개 품종(17.6%)은 중도저 항성을 보였다. 그리고 나머지 40개 품종(39.3%)은 감수성을 나타냈다. 그리고 뿌리혹선충(M. incognita)에 의해 발생하는 고추 뿌리혹선충병에 대한 효율적인 저항성 검정법을 확립하기 위하여 실험한 품종 중 저항성 정도가 다른 6개 품종을 선 발하였다. 그리고 이들 6개 고추 품종의 접종원 농도, 고추 생육 시기 및 이식 후 배양 기간 등의 발병 조건에 따른 뿌리혹 선충에 대한 저항성 차이를 조사하였다. 발병 조건에 따른 품종들의 저항성 차이 결과들을 바탕으로 M. incognita에 대한 고추의 저항성 정도를 검정하기 위한 효율적인 방법으로 온실(25 ± 5℃)에서 21일 동안 재배한 고추 유묘를 원예용 상토 와 모래의 혼합토(1:1, v/v)에 이식하고 7일 동안 재배한 고추 유묘에 뿌리혹선충 M. incognita 알 5,000개를 접종하고 온실 에서 45-60일 동안 재배하고, 뿌리에 형성된 난낭의 수를 조사하여 감수성 대조 품종인 ‘PR 희망찬’에 형성된 난낭 수와 비교하여 저항성을 판정하는 방법을 제안한다.

키워드
난낭
뿌리혹선충
육종
저항성품종
후대생산
References
1
Barker KR, Schmitt DP, Imbriani JL (1985) Nematode population dynamics with emphasis on determining damage potential to crops.In KR Barker, CC Carter, JN Sasser, eds, An Advanced Treatise on Meloidogyne, Vol. II. North Carolina State University, Raleigh, North Carolina, USA, pp 135-148
2
Boina, ANE, Lewis EE, Bloomquist JR (2008) Nematicidal activity of anion transport blockers against Meloidogyne incognita,Caenorhabditis elegans and Heterorhabditis bacteriophora. Pest Manag Sci 64:646-653. doi:10.1002/ps.1591
3
Bosland, PW (1996) Capsicums: Innovative uses of an ancient crop. In J Janick, ed, Progress in New Crops. ASHS Press, Arlington, VA,USA, pp 479-487
4
Bridge J, Page SLJ (1980) Estimation of root-knot nematode infestation levels on roots using a rating chart. Trop Pest Manag 26:296-298. doi:10.1080/09670878009414416
5
Chen ZX, Dickson DW, McSorley R, Mitchell DJ, Hewlett TE (1996) Suppression of Meloidogyne arenaria race 1 by soil application of endospores of Pasteuria penetrans . J Nematol 28:159-168
6
Cho HJ, Han SC, Choi DR (1986) Screening peanut, pepper, cucumber, and tomato varieties for resistance to root-knot nematode,Meloidogyne hapla. Res Rept RDA (P M&U) 28:94-97
7
Choi DR, Lee JK, Park BY, Chung MN (2006) Occurrence of root-knot nematodes in sweet potato fields and resistance screening of sweet potato cultivars. Korean J Appl Entomol 45:211-216 (In Korean)
8
Di Vito M, Saccardo F (1979) Resistance of Capsicum species to Meloidogyne incognita. In F Lamberti, CE Taylor, eds, Root-knot Nematodes (Meloidogyne spp.). Systemics, Biology and Control. Academic Press, London, pp 455-456
9
Djian-Caporalino C, Pijarowski L, Januel A, Lefebvre V, Daubeze A, Palloix A, Dalmasso A, Abad P (1999) Spectrum of resistance to root-knot nematodes and inheritance of heat-stable resistance in pepper (Capsicum annuum L.). Theor Appl Genet 99:496-502. doi:10.1007/s001220051262
10
Djian-Caporalino C, Pijarowski L, Fazari A, Samson M., Gaveau L, O’Byrne C, Lefebvre V, Caranta C, Palloix A, Abad P (2001) Highresolution genetic mapping of the pepper (Capsicum annuum L.) resistance loci Me3 and Me4 conferring heat-stable resistance to root-knot nematodes (Meloidogyne spp.) Theor Appl Genet 103:592-600. doi:10.1007/PL00002914
11
Djian-Caporalino C, Fazari A, Arguel MJ, Vernie T, VandeC-asteele C, Faure I, Brunoud G, Pijarowski L, Palloix A, Lefebvre V, Abad P(2007) Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome. Theor Appl Genet 114:473-486. doi:10.1007/s00122-006-0447-3
12
Fassuliotis G (1985) The role of nematologist in the development of resistance cultivars. In JN Sasser, CC Cater, eds, An Advanced Treaties on Meloidogyne. Vol. I: Biology and Control. North Carolina State University, Raleigh, North Carolina, USA, pp 233-240
13
Gisbert C, Trujillo-Moya C, Sanchez-Torres P, Sifres A, Sanchez-Castro E, Nuez F (2013) Resistance of pepper germplasm to Meloidogyne incognita. Ann Appl Biol 162:110-118. doi:10.1111/aab.12006
14
Han SC, Kim YG (1997) Screening resistant red repper varieties to Meloidogyne hapla and their resistance mechanisms. Korean J ApplEntomol 36:185-191 (In Korean)
15
Hare WW (1956) Resistance to root-knot nematodes in pepper. Phytopathology 46:98-104
16
Hare WW (1957) Inheritance of resistance to root-knot nematodes in pepper. Phytopathology 47:455-459
17
Heald CM, Robinson AF (1987) Effects of soil solarization of Rotylenchus reniformis in the lower Rio Grande Valley of Texas. J Nematol 19:93-103
18
Hwang BK (2002) Studies of resistance of pepper to Phytophthora blight and its control. Res Plant Dis 8:131-145 (In Korean).doi:10.5423/RPD.2002.8.3.131
19
Hwang SM, Park MS, Kim J-C, Jang KS, Choi YH, Choi GJ (2014) Occurrence of Meloidogyne incognita infecting tomato Mi cultivars and development of an efficient screening method for resistant tomato to the Mi-virulent nematode. Korean J Hortic Sci Technol 32:217-226 (In Korean)
20
Kim DG (2001) Occurrence of root-knot nematodes on fruit vegetables under greenhouse conditions in Korea. Res Plant Dis 7:69-79(In Korean)
21
Kim DG, Lee JH (2008) Economic threshold of Meloidogyne incognita for greenhouse grown cucumber in Korea. Res Plant Dis 14:117-121 (In Korean). doi:10.5423/RPD.2008.14.2.117
22
Kim DG, Kwon TY, Ryu YH, Yeon IK, Huh CS (2012) Resistance of commercial pepper cultivars to root-knot nematodes. Res Plant Dis 18:370-375 (In Korean). doi:10.5423/RPD.2012.18.1.029
23
Kim JI, Han SC (1988) Effect of solarization for control of root-knot nematode (Meloidogyne spp.) in vinyl house. Korean J ApplEntomol 27:1-5 (In Korean)
24
Kim S, Kim KT, Chae Y, Jamal A, Oh DG (2008) A survey of genes differently expressed in response to Colletotrichum infection on chilipepper fruit. Korean J Hortic Sci Technol 26:1-8 (In Korean)
25
Lefebvre V, Pflieger S, Thabuis A, Caranta C, Blattes A, Chauvet JC, Daubeze AM, Palloix A (2002) Towards the saturation of the pepper linkage map by alignment of three intraspecific maps including known-function genes. Genome 45:839-854. doi:10.1139/g02-053
26
Meng QP, Long H, Xu JH (2004) PCR assays for rapid and sensitive identification of three major root-knot nematodes, Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria. Acta Phytopathol Sinica 34:204-210
27
Oka Y, Offenbach R, Pivonia, S (2004) Pepper rootstock graft compatibility and response to Meloidogyne javanica and M. incognita. JNematol 36:137-141
28
Pae DH, Chae Y, Wang TC, Engle LM, Shanmugasundaram S (2005) Selection of new breeding materials with resistance to anthracnose in Capsicum annuum. J Kor Soc Hort Sci 46:10-12 (In Korean)
29
Park SD, Kwon TY, Choi BS, Lee WS, Choi YE (1995a) Studies on integrated control against root-knot nematode of fruit vegetable(oriental melon and cucumber) in vinyl house. Korean J Appl Entomol 34:75-81 (In Korean)
30
Park SD, Kwon TY, Jun HS, Choi BS (1995b) The occurrence and severity of damage by root-knot nematode (Meloidogyne incognita)in controlled fruit vegetable field. RDA J Agric Sci 37:318-323 (In Korean)
31
Pegard A, Brizzard G, Fazari A, Soucaze O, Abad P, Djian-Caporalino C (2005) Histological characterization of resistance to different root-knot nematode species related to phenolics accumulation in Capsicum annuum L. Phytopathology 95:158-165. doi:10.1094/ PHYTO-95-0158
32
Rhoades HL (1976) Effects of Indigofera hirsuta on Belonolaimus longicaudatus, Meloidogyne incognita , and M. javanica and subsequent crop yield. Plant Dis Rep 60:384-386
33
Tesarova B, Zouhar M, Rysanek P (2003) Development of PCR for specific determination of root-knot nematode Meloidogyne incognita . Plant Prot Sci 39:23-28
34
The Korean Society of Plant Pathology (KSPP) (2009) Chili pepper. In W-G Kim, HM Koo, eds, List of Plant Disease in Korea. Ed 5,KSPP, Suwon, Korea, pp 73-80
35
Thies JA, Ariss J (2009) Comparison between the N and Me3 genes conferring resistance to the root-knot nematode (Meloidogyne incognita ) in genetically different pepper lines (Capsicum annuum ). Eur J Plant Pathol 125:545-550.doi:10.1007/s10658-009-9502-7
36
Trudgill DL (1991) Resistance to and tolerance of plant parasitic nematodes in plants. Annu Rev Phytopathol 29:167-193. doi:10.1146/annurev.py.29.090191.001123
37
Umesh KC, Ferris H, Bayer DE (1994) Competition between the plant-parasitic nematodes Pratylenchus neglectus and Meloidogyne chitwoodi. J Nematol 26:286-295
38
Yoon JB, Do JW, Yang DC, Park HG (2004) Interspecific cross compatibility among five domesticated species of Capsicum genus. J Kor Soc Hort Sci 45:324-329 (In Korean)
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 34
  • No :2
  • Pages :282-293
  • Received Date : 2015-06-15
  • Revised Date : 2015-08-21
  • Accepted Date : 2016-03-23
  • DOI :https://doi.org/10.12972/kjhst.20160029
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