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Development and Horticultural Evaluation of F1 Hybrids using Microspore-Derived Doubled Haploid Lines and Male-Sterile Lines in Cabbage (Brassica oleracea L. var. capitata)
Subin Choi13
,
Suk Yeon Mo1
,
Byeongjun Park12
,
Han Yong Park1*
1Department of Bioresource Engineering, Sejong University, Seoul 05006, Korea
2Institute of Breeding Research, DASAN Co., Ltd., Pyeongtaek 17864, Korea
3Vegetable Research Division, National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Wanju 55365, Korea
*Corresponding Author
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Abstract

Doubled haploid (DH) lines are useful breeding materials for the rapid development of homozygous parental lines, but their practical application to F1 cabbage breeding depends on stable seed propagation and hybrid performance outcomes. This study evaluated seed production and the crossability of microspore-derived cabbage plants with male-sterile (MS) lines and the horticultural performance of selfed progenies and their F1 hybrids. Among nine microspore-derived plants, six diploid plants produced selfed seeds, whereas three tetraploid plants showed a markedly low seed set, with less than one seed per pod. Crosses between MS lines and diploid microspore-derived plants produced F1 seeds, while diploid × tetraploid crosses failed to produce viable triploid seeds. Field evaluations in Jeju and Yeoju, Korea, revealed that F1 performance outcomes varied depending on the cultivation environment. Based on seed productivity and horticultural traits, SJ-Ca MS 1 × CDH 5 and SJ-Ca MS 3 × CDH 8 were identified as promising F1 combinations for further evaluation. The findings here demonstrate the potential of microspore-derived DH lines as breeding materials for developing F1 cabbage combinations using MS lines.

Keywords
androgenesis
crossability
hybrid breeding
ploidy
seed production
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Received Date : 2026-07-27
  • Revised Date : 2026-10-02
  • Accepted Date : 2026-10-16
  • DOI :https://doi.org/10.7235/HORT.20260053
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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