Effect of After-Ripening,  Cold Stratification,  and GA3 Treatment on Lychnis wilfordii (Regel) Maxim Seed Germination

Sun Hee Ryu; Yong Ha Rhie; Seung Youn Lee; Chung Ho Ko; Jeong Ho Lee; Hae Joo Lee; Ki Cheol Lee

doi:10.12972/kjhst.20170057

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Effect of After-Ripening, Cold Stratification, and GA₃ Treatment on Lychnis wilfordii (Regel) Maxim Seed Germination 후숙, 저온층적 및 GA₃ 처리가 제비동자꽃 종자 발아에 미치는 영향

31 October 2017. pp. 525-533

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Abstract

Lychnis wilfordii (Regel) Maxim is an endemic plant species that occurs in the wetlands of Gangwon-do, Korea. This research was conducted to investigate the effect of after-ripening treatment (0, 3, 6, or 9 weeks at 20-25°C), cold stratification (0, 3, 6, or 9 weeks at 5°C), and GA₃ treatment (0, 10, 100, or 1000 mg·L^-1) on L. wilfordii seed germination. Seeds were transferred to germination conditions (20°C, 12 h photoperiod of florescent lamps with 26.1 μmol·m-2·s-1 PAR or darkness) following after-ripening, cold stratification, or GA₃ treatment. In after-ripening experiments, the final germination percentage (FGP) of seeds with 0, 3, 6, and 9 weeks of after-ripening was 0, 0.7, 2.3, and 33.2% in the light condition and 1.6, 1.9, 2.3, and 22.6% in darkness, respectively. In cold stratification experiments, the FGP of seeds treated with 0, 3, 6, and 9 weeks of clod stratification was 0, 80.5, 77.0, and 83.3% in light conditions and 1.6, 77.8, 58.4, and 83.1% in darkness conditions, respectively. The mean germination time of 1,000 mg·L^-1 GA₃ treatment was approximately five days in light and three days in darkness conditions, respectively. These results indicate that seed germination in L. wilfordii can be improved with 1,000 mg·L^-1 GA₃ and cold stratification (3 or 6 weeks) was effective for the mass propagation of L. wilfordii.

Keywords

Caryophyllaceae

endangered species

ex situ conservation

physiological dormancy

seed propagation

제비동자꽃은 위기종 식물로 주로 강원도 습지에 자생한다. 본 연구에서 제비동자꽃의 현지외 보존과 대량증식법을 개발하고자 종자에 후숙처리(실온에서 0, 3, 6, 9주 처리), 저온층적처리(5°C에서 0, 3, 6, 9주 처리), 그리고 GA₃처리(0, 10, 100, 1000mg·L^-1)가 종자휴면과 발아에 미치는 영향을 알아보았다. 제비동자꽃 종자에 후숙처리, 저온층적처리, 그리고 GA₃처리한 뒤 발아조건으로 옮겨 배양하였다(20°C, 명조건 또는 암조건). 0, 3, 6, 9주 후숙처리 시 각각 명조건에서 0, 0.7, 2.3, 33.2%와 암조건에서 1.6, 1.9, 2.3, 22.6%의 발아율을 보였다. 저온습윤처리(0, 3, 6, 9주) 시 각각의 처리기간에 따라 명조건에서 0, 80.5, 77.0, 83.3%와 암조건에서 1.6, 77.8, 58.4, 그리고 83.1%의 최종발아율을 나타내었다. 0, 10, 100, 그리고 1000mg·L^-1 농도의 GA₃처리 시 명조건에서 각각 0, 0, 0, 56.3%와 암조건에서 1.6, 0.8, 2.7, 73.2%의 발아율을 나타내었다. 그러나 1000mg·L^-1의 GA₃처리 시 평균발아소요일수가 명조건에서 대략 5일, 암조건에서 3일로 단축되었다. 결과적으로 24시간동안 1000mg·L^-1의 GA₃처리와 6주 이상의 저온층적처리를 통하여 제비동자꽃 종자의 발아율을 향상시킬 수 있어 대량증식에 효과적일 것으로 판단된다.

키워드

석죽과

위기종

현지외 보존

대량증식

생리적 휴면

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Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :한국원예학회
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 35
No :5
Pages :525-533
Received Date : 2016-09-19
Revised Date : 2017-02-15
Accepted Date : 2017-01-24
DOI :https://doi.org/10.12972/kjhst.20170057

Horticultural Science and Technology 원예과학기술지 ISSN:1226-8763(Print) 2465-8588(Online)

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