All Issue

2021 Vol.39, Issue 4 Preview Page

Research Article

Detection of Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis in Korea

국내 호접란의 Cymbidium mosaic virus 및 Odontoglossum ringspot virus 감염 현황

Da-Seul Jeong13
,
Kwang Hee Hong2
,
Kyeung-Il Park1*
정 다슬13
,
홍 광희2
,
박 경일1*
1Department of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Korea
2Department of English Language Education, Yeungnam University, Gyeongsan 38541, Korea
3Present affiliations: Exhibition Center Management Office, Sejong National Arboretum, Sejong 30106, Korea
1영남대학교 원예생명과학과
2영남대학교 영어교육학과
3국립세종수목원 전시원관리실
*Corresponding Author
31 August 2021. pp. 514-520
PDF XML Citation
Abstract

The primary purpose of this study was to examine the effects of virus infection in Phalaenopsis cultivated across the country. RT-PCR analysis was used to detect two major viruses in orchid: CymMV and ORSV. The virus infection rate of in vitro plantlets from 45 cultivars was 83.6%, and the rate of potted plants from 21 cultivars was 92.5%. For CymMV, single infection in in vitro plantlets was 34.3%, and for ORSV, it was 11.4%. In the case of potted plants, single infection with CymMV was 57.0%, and ORSV alone was not detected. For double infection with both viruses, in vitro plants accounted for 37.9% and potted plants for 35.5%. No infection with both viruses was found in in vitro plantlets with ‘V3’ imported from Taiwan. Plants infected with CymMV showed chlorotic and necrotic spots on leaves and color break in petals and sepals of flowers, and those infected with both CymMV and ORSV featured yellow striped or mosaic symptoms.

Keywords
CymMV
DN method
in vitro plantlets
ORSV
potted plants
RT-PCR

이 연구는 국내에서 재배하는 호접란의 바이러스 감염정도를 조사하기 위해 수행되었다. CymMV, ORSV 두 바이러스를 검정하기 위해 RT-PCR 검사를 진행하였다. 45품종의 기내배양묘의 바이러스 감염률은 83.6%였으며 21품종의 분화묘의 감염률은 92.5%였다. 기내배양묘의 CymMV 단독감염률은 34.3%이고 ORSV 단독감염률은 11.4%이다. 분화묘의 경우, CymMV의 단독감염률은 57.0%였으나 ORSV 단독감염은 확인되지 않았다. 두 바이러스에 대한 복합감염률은 기내배양묘와 분화묘에서 각각 37.9%, 35.5%였다. 대만 수입 ‘V3’ 품종 기내배양묘에서는 두 바이러스에 대한 감염이 확인되지 않았다. CymMV 감염주는 잎의 백화와 괴저반점의 증상을 나타냈고 꽃잎과 꽃받침의 화색변이를 보였다. 그리고 CymMV와 ORSV의 중복감염주는 퇴록 줄무늬 또는 얼룩무늬 증상을 보였다.

키워드
CymMV
DN method
기내배양묘
ORSV
분화묘
RT-PCR
References
1
Animal and Plant Quarantine Agency (APQA) (2017) Notice No.2017-64 http://www.qia.go.kr/bbs/lawAnn/viewLawWebAction.do?id=151532&type=0. Accessed 9 May 2020
2
Chang MU (1998) Viral diseases occurring in orchid plants and the control methods. Korean J Plant Pathol 4:1-18
3
Chang MU, Chun HH, Baek DH, Chanung JD (1991a) Studies on the viruses in orchids in Korea, 1; Bean yellow mosaic virus, cucumber mosaic virus, cymbidium mild mosaic virus, and cymbidium mosaic virus. Korean J Plant Pathol 7:108-117
4
Chang MU, Chun HH, Baek DH, Chanung JD (1991b) Studies on the viruses in orchids in Korea, 2; Dendrobium mosaic virus, Odontoglossum ringspot virus, Orchid fleck virus, and Unidentified potyvirus. Korean J Plant Pathol 7:118-129
5
Chung BN, Yoon JY, Kim MS (2010) Viral infection of tissue cultured orchids and evaluation of damages. Plant Pathol J 26:194-197. doi:10.5423/PPJ.2010.26.2.194 10.5423/PPJ.2010.26.2.194
6
Fulton RW (1986) Practices and precautions in the use of cross protection for plant virus disease control. Ann Rev Phytopathol 24:67-81. doi:10.1146/annurev.py.24.090186.000435 10.1146/annurev.py.24.090186.000435
7
Hollings M (1965) Disease control through virus-free stock. Ann Rev Phytopathol 3:367-396. doi:10.1146/annurev.py.03.090165.002055 10.1146/annurev.py.03.090165.002055
8
Koh KW, Lu HC, Chan MT (2014) Virus resistance in orchids. Plant Sci 228:26-38. doi:10.1016/j.plantsci.2014.04.015 10.1016/j.plantsci.2014.04.015
9
Korea Agro-Fisheries & Food Trade Corporation (aT) (2015-2017) aT monthly market reviews. http://www.at.or.kr/article/fmko333000/list.action. Accessed 5 June 2019
10
Kwon AR, Oh MM, Paek KY, Park SY (2018) The effect of light quality on growth and endopolyploidy occurrence of in vitro-grown Phalaenopsis 'Spring Dancer'. Hortic Environ Biotechnol 59:179-188. doi:10.1007/s13580-018-0018-y 10.1007/s13580-018-0018-y
11
Lee HB, Lee JH, An SK, Park JH, Kim KS (2019) Growth characteristics and flowering initiation of Phalaenopsis Queen Beer 'Mantefon' as affected by the daily light integral. Hortic Environ Biotechnol 60:637-645. doi:10.1007/s13580-019-00156-2 10.1007/s13580-019-00156-2
12
Lee HB, Lim SH, Lim NH, An SK, Kim KS (2018) Growth and CO2 exchange in young Phalaenopsis orchids grown under different levels of humidity during the vegetative period. Hortic Environ Biotechnol 59:37-43. doi:10.1007/s13580-018-0005-3 10.1007/s13580-018-0005-3
13
Ministry of Agriculture, Food and Rural Affairs (MAFRA) (2019) Cultivation status of flowering plants in Korea 2018. http://lib.mafra.go.kr/Search/Detail/45582. Accessed 9 May 2020
14
Navalinskienë M, Raugalas J, Samuitienë M (2005) Viral diseases of flower plants 16. Identification of viruses affecting orchids (Cymbidium SW.). Biologija, pp 29-34
15
Park WM, Shim KB, Kim SJ, Ryu KH (1998) Detection of Cymbidium mosaic virus and Odontoglossum ringspot virus by ELISA and RT-PCR from cultivated orchids in Korea. Korean J Plant Pathol 14:130-135
16
Ryu KH, Kim SJ, Park WM (1998) Incidence of Cymbidium mosaic virus and Odontoglossum ringspot virus in Cymbidium and other orchids in Korea. J Kor Soc Hortic Sci 39:213-217
17
Yonhap News Agency (2019) Exports the potted phalaenopsis of Taean to the U.S. "First time in Korea". https://www.yna.co.kr/view/AKR20190310022800063. Accessed 10 March 2019
18
Yun JS, Hong ET, Kim IH, Yun T, Kim TS, Paek KY (2000) Detection of Cymbidium mosaic virus and Odontoglossum ringspot virus in seed-derived plantlets of Phalaenopsis imported by one-step RT-PCR. Korean J Hortic Sci Technol 18:513-517
19
Zettler FW, Ko NJ, Wisler GC, Elliott MS, Wong SM (1990) Viruses of orchids and their control. Plant Dis 74:621-626. doi:10.1094/PD-74-0621 10.1094/PD-74-0621
20
Zheng YX, Shen BN, Chen CC, Jan FJ (2010) Odontoglossum ringspot virus causing flower crinkle in Phalaenopsis hybrids. Eur J Plant Pathol 128:1-5. doi:10.1007/s10658-010-9630-0 10.1007/s10658-010-9630-0
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :4
  • Pages :514-520
  • Received Date : 2020-10-12
  • Revised Date : 2021-02-01
  • Accepted Date : 2021-03-09
  • DOI :https://doi.org/10.7235/HORT.20210046
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
Online Submission
  • scopus
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close