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2018 Vol.36, Issue 4 Preview Page

Research Article

A Phylogenic Analysis of Citrus Cultivars Native to Jeju using Chloroplast DNA trnL-trnF and Internal Transcribed Spacer Region Sequences

엽록체 DNA trnL-trnF와 ITS 영역의 염기서열을 이용한 제주도 재래감귤 품종들의 계통학적 유연관계 분석

Seong Beom Jin1
,
Won Jeong Lee1
,
Jae Ho Park1
,
Suk Man Park2
,
Dong Hoon Lee3
,
Su Hyun Yun1*
진 성범1
,
이 원정1
,
박 재호1
,
박 석만2
,
이 동훈3
,
윤 수현1*
1Citrus Research Institute, National Institute of Horticultural and Herbal Science
2R&D Coordination Division, Rural Development Administration
3Planning and Coordination Division, National Institute of Horticultural and Herbal Science
1농촌진흥청 국립원예특작과학원 감귤연구소
2농촌진흥청 연구정책국 연구운영과
3농촌진흥청 국립원예특작과학원 기획조정과
*교신저자. *Corresponding Author. 
31 August 2018. pp. 585-597
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Abstract

This study was carried out to determine the phylogenetic relationships among 28 Citrus species, including 11 cultivars native to Jeju, Korea, based on the sequences of the trnL-trnF region in chloroplast DNA and the internal transcribed spacer (ITS) region in nuclear ribosomal DNA. The average total length of the trnL-F region in chloroplast DNA was 361.1 bp, and that of the conserved sequence site was 354 bp, that of the variable site was 7 bp, and that of the parsimonious informative site was 2 bp. The average total length of the ITS region, which contains the 5.8S rDNA region, was 637.68 bp, and that of the conserved sequence site was 570 bp, that of the variable site was 68 bp, and that of the parsimonious informative site was 25 bp. Analysis of sequence divergence and phylogenetic relationships among the citrus species revealed that most native citrus cultivars were closely related, and these cultivars were presumed to be obtained by cross-breeding with pomelo (Citrus maxima) and mandarin (C. reticulata). In particular, the ‘Byungkyool’ cultivars, presumed to be a unique cultivar genetically and morphologically selected on Jeju island, was confirmed to have been obtained by long-term hybridization with cultivars of pomelo (‘Dangyooja’, etc.) and mandarin (‘Hongkyool’, etc.). However, analysis of the phylogenetic relationships among citrus cultivars native to Jeju was difficult due to the propagation through seedling from unknown cross combinations long time ago. These results suggest that phylogenetic analysis could be used to generate basic data about native citrus cultivars on Jeju island.

Keywords
cross-breeding
hybridization
parsimonious informative
Rutaceae
sequence divergence

이 연구는 엽록체 DNA 내 trnL-F와 핵 리보솜 DNA내 ITS 영역의 염기서열을 근거로 제주도 재래감귤 11품종들을 포함한 전체 28종의 감귤에 대한 계통분류학적 연구를 수행하였다. 엽록체 DNA내 trnL-F 영역의 염기의 평균적 총 길이는 361.1bp이 며, 염기의 변이가 없이 보존되는 site는 354개, 변이를 보이는 site는 7개였고, 이 중 2개의 염기가 유효한 것으로 나타났다. ITS 전체(ITS1-5.8S-ITS2) 영역 내 염기의 평균적 총 길이는 637.68bp이며, 염기의 변이가 없이 보존되는 site는 570개, 염기의 변 이를 보이는 site는 68개였고, 이 중 25개의 염기가 유효한 것으로 나타났다. 엽록체 DNA 내 trnL-F 영역과 전체 ITS 영역의 염 기서열을 이용하여 분기값과 계통관계를 분석한 결과, 대다수의 재래감귤 품종들은 유전적으로 근연관계에 있었으며 문단과 만다린과의 잡종에 의하여 얻어진 품종이라고 추정할 수 있다. 특히, 유전적, 형태학적으로 제주도 내 유일 품종이라고 추측되 고 있는 ‘병귤’은 문단(‘당유자’ 등)과 만다린(‘홍귤’ 등) 품종들과의 오랜 기간 동안 교잡에 의하여 얻어진 품종이라는 것을 확 인할 수 있었다. 하지만 재래감귤은 재배된 시기와 주변 품종이 다르기 때문에 품종들 간의 계통분류학적 관계를 분석하는 것 은 쉽지가 않다. 따라서, 이 연구 결과는 제주도 내 재래감귤 품종들의 계통관계 분석함에 있어서 기초자료로 사용 가능하리라 생각된다.

키워드
잡종
교잡
유효 염기
운향과
분기값
References
1
Banihashemi Z, DeZeeuw DJ (1975) The behavior of Fusarium oxysporum f. sp. melonis in the presence and absence of host plants. Phytopathology 65:1212-1217. doi:10.1094/Phyto-65-1212
2
Barksdale TH, Papavizas GC, Johnston SA (1984) Resistance to foliar blight and crown rot of pepper caused by Phytophthora capsici. Plant Dis 68:506-509. doi:10.1094/PD-68-506
3
Bouhot D (1981) Some aspects of the pathogenic potential in formae speciales and races of Fusarium oxysporum on Cucurbitaceae. In PE Nelson, TA Toussoun, RJ Cook, eds, Fusarium: Disease, Biology, and Taxonomy. Pennsylvania State University Press, University Park, USA, pp 318-326
4
Cohen R, Horev C, Burger Y, Shraiber S, Hershenhorn J, Katan J, Edelstein M (2002) Horticultural and pathological aspects of Fusarium wilt management using grafted melons. HortScience 37:1069-1073
5
Crino p, Lo Bianco C, Rouphael Y, Colla G, Saccardo F, Paratore A (2007) Evaluation of rootstock resistance to Fusarium wilt and gummy stem blight and effect on yield and quality of a grafted ‘Inodorus’ melon. HortScience 42:521-525
6
Freeman S, Zveibil A, Vintal H, Maymon M (2002) Isolation of nonpathogenic mutants of Fusarium oxysporum f. sp. melonis for biological control of Fusarium wilt in cucurbits. Phytopathology 92:164-168. doi:10.1094/PHYTO.2002.92.2.164
7
Herman R, Perl-Treves R (2007) Characterization and inheritance of a new source of resistance to Fusarium oxysporum f. sp. melonis Race 1,2 in Cucumis melo. Plant Dis 91:1180-1186. doi:10.1094/PDIS-91-9-1180
8
Jo E J, Jang KS, Choi YH, Ahn KG, Choi GJ (2016) Resistance of cabbage plants to isolates of Plasmodiophora brassicae. Korean J Hortic Sci Technol 34:442-452
9
Jo S-J, Shim S-A, Jang KS, Choi YH, Kim J-C, Choi GJ (2014) Resistance of chili pepper cultivars to isolates of Phytophthora capsici. Korean J Hortic Sci Technol 32:66-76. doi:10.7235/hort.2014.13079
10
Katan T, Katan J, Gordon TR, Pozniak D (1994) Physiologic races and vegetative compatibility groups of Fusarium oxysporum f. sp. melonis in Israel. Phytopathology 84:153–153. doi:10.1094/Phyto-84-153
11
Kim DK, Seo SG, Kwon SB, Park YD (2016) Development of RAPD and SCAR markers related to watermelon mosaic virus and zucchini yellow mosaic virus resistance in Cucurbita moschata. Hortic Environ Biotechnol 57:61-68
12
Kim YJ, Hwang BK, Park KW (1989) Expression of age-related resistance in pepper plants infected with Phytophthora capsici. Plant Dis 73:745-747. doi:10.1094/PD-73-0745
13
King SR, Davis AR, Liu W, Levi A (2008) Grafting for disease resistance. HortScience 43:1673-1676
14
Lee JM (2013) Oriental melon and melon. In Vegetable Sciences Crop Details. Hyangmunsa, Seoul, pp 62-91
15
Lee WJ, Jang KS, Choi YH, Kim HT, Kim J-C, Choi GJ (2015a) Development of an efficient simple mass-screening method for resistant melon to Fusarium oxysporum f. sp. melonis. Res Plant Dis 21:201-207. doi:10.5423/RPD.2015.21.3.201
16
Lee WJ, Lee JH, Jang KS, Choi YH, Kim HT, Choi GJ (2015b) Development of efficient screening methods for melon plants resistant to Fusarium oxysporum f. sp. melonis. Korean J Hortic Sci Technol 33:70-82. doi:10.7235/hort.2015.14101
17
MAFRA (Ministry of Agriculture, Food, and Rural Affairs) (2016) Agriculture, Food, and Rural Affairs Statistic Yearbook, 2015. MAFRA, Sejong, Korea
18
Martyn RD, Gordon TR (2017) Fusarium wilt of melon. In AP Keinath, WM Wintermantel, TA Zitter, eds, Compendium of Cucurbit Diseases, 2nd Ed, American Phytopathological Society Press, St. Paul, MN, USA, pp 36-38
19
Matsumoto Y, Ogawara T, Miyagi M, Watanabe N and Kuboyama T (2011) Response of wild Cucumis species to inoculation with Fusarium oxysporum f. sp. melonis race 1,2y. J Jpn Soc Hortic Sci 80:414-419. doi:10.2503/jjshs1.80.414
20
Messiaen CM, Risser G, Pecaut P (1962) Etude des plantes resistantes au Fusarium oxysporum f. sp. melonis dans la variete de melon Cantaloup Charentais. Ann Amelior Plantes 12:157-164
21
Namiki F, Shimizu K, Satoh K, Hirabayashi T, Nishi K, Kayamura T, Tsuge T (2000) Occurrence of Fusarium oxysporum f. sp. melonis race 1 in Japan. J Gen Plant Pathol 66:12-17. doi:10.1007/PL00012915
22
Namiki F, Shiomi T, Nishi K, Kayamura T, Tsuge T (1998) Pathogenic and genetic variation in the Japanese strains of Fusarium oxysporum f. sp. melonis. Phytopathology 88:804-810. doi:10.1094/PHYTO.1998.88.8.804
23
Oumouloud A, El-Otmanii M, Chikh-Rouhou H, Garces Claver A, Gonzalez Torres R, Perl-Treves R, Alvarez JM (2013) Breeding melon for resistance to Fusarium wilt: recent develoments. Euphytica 192:155-169. doi:10.1007/s10681-013-0904-4
24
Park SD, Kwon TY, Lim YS, Jung KC, Choi BS (1996) Disease survey in melon, watermelon, and cucumber with different successive cropping periods under vinyl house conditions. Korean J Plant Pathol 12:428-431
25
Park SW, Kim KT, Kang SC, Yang HB (2016) Rapid and practical molecular marker development for rind traits in watermelon. Hortic Environ Biotechnol 57:385-391
26
Perchepied L, Pitrat M (2004) Polygenic inheritance of partial resistance to Fusarium oxysporum f. sp. melonis race 1,2 in melon. Phytopathology 94:1331-1336. doi:10.1094/PHYTO.2004.94.12.1331
27
Risser G, Banihashemi Z, Davis DW (1976) A proposed nomenclature of Fusarium oxysporum f. sp. melonis races and resistance genes in Cucumis melo. Phytopathology 66:1105-1106. doi:10.1094/Phyto-66-1105
28
Shlevin E, Mahrer Y, Krizman G, Katan J (2004) Survival of plant pathogens under structural solarization. Phytoparasitica 32:470-478. doi:10.1007/BF02980441
29
Tamietti G, Valentino D (2006) Soil solarization as an ecological method for the control of Fusarium wilt of melon in Italy. Crop Prot 25:389-397. doi:10.1016/j.cropro.2005.07.002
30
Veloso MM, Melo EMPF, Jorge-Silva ML, Bravo MA (2000) Genetic diversity in Fusarium oxysporum f. sp. melonis. EPPO Bull 30:195-197. doi:10.1111/j.1365-2338.2000.tb00878.x
31
Zink FW (1992) Genetics of resistance to Fusarium oxysporum f. sp. melonis races 0 and 2 in muskmelon cultivars Honey Dew, Iroquois, and Delicious 51. Plant Dis 76:162-166. doi:10.1094/PD-76-0162
32
Zink FW, Thomas CE (1990) Genetics of resistance to Fusarium oxysporum f. sp. melonis races 0, 1, and 2 in muskmelon line MR-1. Plant Dis 80:1230-1232
33
Zitter TA (1999) Fusarium wilt of melon, a worldwide problem in temperate and tropical regions. Acta Hortic 492:157-160. doi:10.17660/ActaHortic.1999.492.18
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 36
  • No :4
  • Pages :585-597
  • Received Date : 2017-08-03
  • Revised Date : 2017-11-11
  • Accepted Date : 2017-11-10
  • DOI :https://doi.org/10.12972/kjhst.20180059
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