All Issue

2017 Vol.35, Issue 2 Preview Page
Development of an Effective Method for Testing Resistance to Black Spot of Radish Caused by Alternaria brassicicola

Alternaria brassicicola에 의한 무 검은무늬병에 대한 효율적인 저항성 검정법 개발

Ji Hyun Lee1
,
Kyoung Soo Jang1
,
Yong Ho Choi1
,
Hun Kim1
,
Gyung Ja Choi1*
이 지현1
,
장 경수1
,
최 용호1
,
김 헌1
,
최 경자1*
1Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology
1한국화학연구원 친환경신물질연구센터
*교신저자. *Corresponding Author. 
30 April 2017. pp. 210-219
PDF XML Citation
Abstract

This study was conducted to establish an efficient screening method for radish (Raphanus sativus ) cultivars that are resistant to black spot, which is caused by Alternaria brassicicola . Seven A. brassicicola isolates were selected and investigated for their ability to produce spores and pathogenicity. Of these isolates, A. brassicicola KACC 40036 and 43923 produced abundant spores in V-8 juice agar medium and showed pathogenicity and strong virulence on radish seedlings. We examined the resistance of 61 commercial cultivars of radish to A. brassicicola KACC40036, and found that there are no highly resistant radish cultivars; however, some cultivars, such as ‘Geumbong’ and ‘Searom’, showed weak resistance to A. brassicicola . For further study, we selected four radish cultivars that showed different disease responses to A. brassicicola KACC40036. According to the growth stage of the radish seedlings, inoculum concentration, and incubation temperature of radish, development of black spot on four cultivars has been investigated. The results showed that younger seedlings were more sensitive to A. brassicicola than older seedlings, and the disease severity depended on the concentration of the spore suspension. The disease severity of plants incubated in humidity chamber at 25°C was greater than that of plants grown at 20°C or 30°C. Taken together, we suggest the following method for screening for radish plants that are resistant to A. brassicicola : 1) inoculate 16-dayold radish seedlings with an A. brassicicola spore suspension (2.0 × 105 spores·mL-1) using the spray method, 2) incubate the inoculated plants in a humidity chamber at 25°C for 24 h and then transfer the plants to a growth chamber at 25°C with 80% relative humidity under a 12 h light/ dark cycle, and 3) assess the disease severity of the plants two days after inoculation.

Keywords
breeding
disease resistance
inoculation
Raphanus sativus

본 연구는 Alternaria brassicicola에 의해 발생하는 무 검은무늬병에 대한 효율적인 저항성 검정법을 확립하기 위하여 수행 되었다. A. brassicicola 7균주의 포자 형성량과 병원성을 검정하였다. 실험한 균주 중, A. brassicicola KACC 40036과 43923 균주는 V-8 juice agar 배지에서 가장 많은 포자를 생산하였다. 그리고 실험한 균주 모두는 무 유묘에 대하여 강한 병원력을 나 타냈다. 시판 중인 무 61개 품종의 A. brassicicola KACC 40036 균주에 대한 저항성 스크리닝을 수행하였다. 실험한 품종 중 높은 저항성을 보이는 품종은 없었으나 ‘금봉’과 ‘새롬’같이 약한 저항성을 나타내는 품종이 발견되었다. 이들 결과로부터 추후 실험을 위해 저항성 반응에 차이를 보이는 4개 품종을 선발하였다. 이들 4개 품종의 접종하는 무의 생육 시기, 접종원 농도 그 리고 접종 후의 재배 온도에 따른 검은무늬병 발생을 조사하였다. 실험한 모든 품종은 어린 유묘일수록 검은무늬병에 대한 감 수성이 증가하였으며, 더 많은 A. brassicicola 포자를 접종할수록 검은무늬병이 더 많이 발생하였다. 그리고 접종한 유묘를 25°C의 습실상에서 배양하였을 때에는 20°C 나 30°C에서 배양하였을 때보다 검은무늬병 발생이 증가하였다. 이들 실험의 결 과로부터 무 품종의 검은무늬병에 대한 저항성을 검정하기 위해서는 무 종자를 파종하고 온실(25 ± 5°C)에서 16일 동안 재배 하고, 이 유묘에 A. brassicicola 균주의 포자현탁액(2.0 × 105 spores·mL-1)을 분무하여 접종하고, 접종한 식물은 25°C 습실상 에 24시간 동안 배양한 후에 25°C, 상대습도 80%의 생육상에서 재배하고, 접종 2일 후에 병반면적율을 조사하는 것을 제안한다.

키워드
육종
병 저항성
접종R
aphanus sativus
References
1
Agrios GN (2005) Plant diseases caused by fungi. In Plant Pathology, Ed 5. Academic Press Ltd., San Diego, CA, USA, pp 385-614. doi:10.1016/b978-0-08-047378-9.50017-8
2
Babadoost M, Gabrielson RL (1979) Pathogens causing Alternaria diseases of brassica seed crops in western Washington. Plant Dis Reptr 63:815-820
3
Bae K-M, Sim S-C, Hong J-H, Choi K-J, Kim D-H, Kwon Y-S (2015) Development of genomic SSR markers and genetic diversity analysis in cultivated radish (Raphanus sativus L.). Hortic Environ Biotechnol 56:216-224. doi:10.1007/s13580-015-0089-y
4
Bassey EO, Gabrielson RL (1983) The effects of humidity, seed infection level, temperature and nutrient stress on cabbage seedling disease caused by Alternaria brassicicola. Seed Sci Technol 11:403-410
5
Conn KL, Tewari JP, Dahiya JS (1988) Resistance to Alternaria brassicae and phytoalexin-elicitation in rapeseed and other crucifers.Plant Sci 56:21-25. doi:10.1016/0168-9452(88)90180-X
6
Doullah MAU, Meah MB, Okazaki K (2006) Development of an effective screening method for partial resistance to Alternaria brassicicola (dark leaf spot) in Brassica rapa . Eur J Plant Pathol 116:33-43. doi:10.1007/s10658-006-9035-2
7
Hong CX, Fitt BDL (1995) Effect of inoculum concentration, leaf age and wetness period on the development of dark leaf and pod spot (Alternaria brassicae ) on oilseed rape (Brassica napus ). Ann Appl Biol 127:283-295. doi:10.1111/j.1744- 7348.1995.tb06673.x
8
Hong CX, Fitt BDL, Welham SJ (1996) Effect of wetness period and temperature on development of dark pod spot (Alternaria brassicae ) on oilseed rape (Brassica napus ). Plant Pathol 45:1077-1089. doi:10.1046/j.1365-3059.1996.d01-193.x
9
Iacomi-Vasilescu B, Avenot H, Bataillé -Simoneau N, Laurent E, Gué nard M, Simoneau P (2004) In vitro fungicide sensitivity of Alternaria species pathogenic to crucifers and identification of Alternaria brassicicola field isolates highly resistant to both dicarboximides and phenylpyrroles. Crop Protect 23:481-488. doi:10.1016/j.cropro.2003.10.003
10
Jo EJ, Lee JH, Choi YH, Kim J-C, Choi G J (2015) Development of an efficient method of screening for watermelon plants resistant to Fusarium oxysporum f. sp. niveum . Korean J Hortic Sci Technol 33:409-419. doi:10.7235/hort.2015.14156
11
Jo S-J, Shim S-A, Jang KS, Choi YH, Kim J-C, Choi G J (2014) Resistance of chili pepper cultivars to isolates of Phytophthora capsici . Korean J Hortic Sci Technol 32:66-76. doi:10.7235/hort.2014.13079
12
Kim J, Kim D-S, Park S, Lee H-E, Ahn Y-K, Kim JH, Yang H-B, Kang B-C (2016) Development of a high-throughput SNP marker set by transcriptome sequencing to accelerate genetic background selection in Brassica rapa . Hortic Environ Biotechnol 57:280-290. doi:10.1007/s13580-016-1036-2
13
King SR (1994) Screening, selection, and genetics of resistance to Alternaria diseases in Brassica oleracea . Ph.D. thesis, Cornell University, Ithaca, New York
14
Kö hl J, van Tongeren CAM, Groenenboom-de Haas BH, van Hoof RA, Driessen R, van der Heijden L. (2010) Epidemiology of dark leaf spot caused by Alternaria brassicicola and A. brassicae in organic seed production of cauliflower. Plant Pathol 59:358-367. doi:10.1111/j.1365-3059.2009.02216.x
15
Korean Society of Plant Pathology (KSPP) (2009) Vegetables. In W-G Kim, HM Koo, eds, List of Plant Disease in Korea, Ed 5. KSPP, Suwon, Korea, pp 99-103
16
Kumar D, Maurya N, Bharati YK, Kumar A, Kumar K, Srivastava K, Chand G, Kushwaha C, Singh SK, Mishra RK, Kuma A (2014) Alternaria blight of oilseed Brassicas: A comprehensive review. Afr J Microbiol Res 8:2816-2829. doi:10.5897/AJMR2013.6434
17
Lee HB, Yu SH (1995) Distribution of mycotoxin-producing isolates in the genus Alternaria . Korean J Plant Pathol 11:151-157 (In Korean)
18
Mora A, Earle ED (2001) Combination of Trichoderma harzianum endochitinase and a membrane-affecting fungicide on control of Alternaria leaf spot in transgenic broccoli plants. Appl Microbiol Biotechnol 55:306-310. doi:10.1007/s002530000496
19
Mridha MAU, Wheeler BEJ (1993) In vitro effects of temperature and wet periods on infection of oilseed rape by Alternaria brassicae . Plant Pathol 42:671-675. doi:10.1111/j.1365-3059.1993.tb01551.x
20
Muto M, Takahashi H, Ishihara K, Yuasa H, Huang JW (2005) Control of black leaf spot (Alternaria brassicicola ) of crucifers by extracts of black nightshade (Solanum nigrum ). Plant Pathol Bull 14:25-34
21
Nowicki M, Nowakowska M, Niezgoda A, Kozik EU (2012) Alternaria black spot of crucifers: symptoms, importance of disease and perspectives of resistance breeding. Vegetable Crops Res Bull 76:5-19. doi:10.2478/v10032-012-0001-6
22
Otani H, Kohnobe A, Kodama M, Kohmoto K (1998) Production of a host-specific toxin by germinating spores of Alternaria brassicicola . Physiol Mol Plant Pathol 52:285-295. doi:10.1006/pmpp.1998.0147
23
Pedras MSC, Chumala PB, Jin W, Islam MS, Hauck DW (2009) The phytopathogenic fungus Alternaria brassicicola : Phytotoxin production and phytoalexin elicitation. Phytochemistry 70:394-402. doi:10.1016/j.phytochem.2009.01.005
24
Tewari JP, Mithen RF (1999) Disease. In C Gó mez-Campo, ed, Biology of Brassica Coenospecies. Elsevier, Amsterdam, Netherlands, pp 375-411. doi:10.1016/S0168-7972(99)80013-9
25
Yu HY, Yun HK, Park CH, Lee HB (1991) Three species of Alternaria associated with Alternaria leaf spot of radish in Korea. Korean J Plant Pathol 7:188-191 (In Korean)
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 35
  • No :2
  • Pages :210-219
  • Received Date : 2016-06-10
  • Revised Date : 2016-10-06
  • Accepted Date : 2016-09-26
  • DOI :https://doi.org/10.12972/kjhst.20170024
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
Online Submission
  • scopus
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close