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2018 Vol.36, Issue 1 Preview Page
Variation inGlucosinolateand PolyphenolLeveland Level of Peroxisome Proliferator Activated Receptorγ(PPARγ)Expression in anEthanolExtract of Highland Kimchi Cabbage

고랭지배추의 Glucosinolate 및 Polyphenol 함량변이와 에탄올 추출물의 퍼옥시솜증식체활성화 수용체γ(PPARγ) 발현 활성

Ki-Deog Kim1*
,
Jun-Gu Lee2
,
Jong-Taek Suh1
,
Jong-Nam Lee1
,
Dong-Lim Yoo1
,
Jung-Hwan Nam1
,
Hwang-Bae Sohn1
,
Su-Young Hong1
,
Yul-Ho Kim1
,
Su-Jeong Kim1
,
Soon-Choon Hong1
,
Jung-Su Jo2
,
Doman Kim3
김 기덕1*
,
이 준구2
,
서 종택1
,
이 종남1
,
유 동림1
,
남 정환1
,
손 황배1
,
홍 수영1
,
김 율호1
,
김 수정1
,
홍 순춘1
,
조 정수2
,
김 도만3
1Highland Agriculture Research Institute, National Institute Crop Science
2Department of Horticulture, Chonbuk National University
3Graduate School of International Studies, Seoul National University
1국립식량과학원 고령지농업연구소
2전북대학교 원예학과
3서울대학교 국제대학원
*교신저자. *Corresponding Author. 
28 February 2018. pp. 128-139
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Abstract

Variations of polyphenol and glucosinolate levels, and growth characteristics of 40 cultivars of Kimchi cabbage cultivated in highlands were analyzed, in combination with the level of per-oxisome proliferator activated receptorγ (PPARγ) expression in ethanol extract from leaves. In addition, the effect of sulfur and nitrogen supply on the sulfur and glucosinolate content was investigated. Differences in the levels of total polyphenol and glucosinolate could be detected in the different cultivars of Kimchi cabbage. Glucosinolate epiprogoitrin was detected in small amounts or not detected, and the level of glucosinolate progoitrin showed clear-differences in different cultivars. The anti-carcinogenic glucosinolate gluconasturttin level in Kimchi cabbage (‘Asiaib-ssam’) leaves was higher than 6.85 μmol·g-1. Sulfur and glucosinolate levels in Kimchi cabbage leaves were dependent on the sulfur supply. The levels of the aliphatic glucosinolates progoitrin, gluconapin and glucobrassicanapin generally decreased with the increase of nitrogen supply, but the indolyl glucosinolate glucobrassicin increased. The total polyphenol levels in Kimchi cabbage leaves ranged from 1.5 to 2.3 mg GAE/g. Leaf ethanol extracts of 40 cultivars of Kimchi cabbage cultivated in highlands, activated the expression of PPARγ 2-4 fold when compared with the negative control.

Keywords
growth
sulfur
nitrogen
progoitrin
glucobrassicanapin

고랭지에서 재배된 배추40종의 생육반응 및 glucosinolate 와 polyphenol 함량의 변이를 분석하였으며, 배추 잎 에탄올 추출물의 퍼옥시솜증식체활성화수용체γ(PPARγ)의활성에 미치는 영향을측정하였다. 그리고 황 및 질소시용에 따른glucosi-nolate 함량 반응을 조사하였다. 배추의 품종에따라polyphenol 및 glucosinolate 의 함량 차이가 나타났다. Glucosinolate epi-progoitrin 은 미량이 포함되어 있거나 검출되지않았으며, 유채에 많이 함유되어 있는progoitrin 은 품종마다 함량 차이가뚜렷하게 나타났다. 항암 효과가 있는 gluconasturttin 은 다른 품종에 비해 아시아잎쌈배추에서 6.85μmol·g-1로 현저하게 높은 것을알 수 있었다. 황 시용량의증가로 식물체 내 황함량과glucosinolate 함량이 증가하였다. 대체로 질소 시용량이 많아지면aliphatic glucosinolate 인 progoitrin, gluconapin 및 glucobrassicanapin 의 함량은 감소하는 경향을 보인 반면 indolyl glucosi-nolate 인 glucobrassicin 은 증가하는 경향을 보였다. 총 polyphenol 함량은 1.5-2.3mg GAE/g 의 범위로 나타났다. 모든 배추 품종의 잎 에탄올 추출물이 PPAR γ 활성을2-4 배 증가시켰다.

키워드
생육
황
질소
progoitrin
glucobrassicanapin
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 36
  • No :1
  • Pages :128-139
  • Received Date : 2017-10-17
  • Revised Date : 2017-05-21
  • Accepted Date : 2017-04-24
  • DOI :https://doi.org/10.12972/kjhst.20180014
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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