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Interspecific Transferability of Watermelon EST-SSRs Assessed by Genetic Relationship Analysis of Cucurbitaceous Crops

박과작물의 유연관계 분석을 통한 수박 EST-SSR 마커의 종간 적용성 검정

Hyeogjun Kim1
,
Sang-Seok Yeo1
,
Dong-Yeop Han1
,
Young-Hoon Park12*
김 혁준1
,
여 상석1
,
한 동엽1
,
박 영훈12*
1Department of Horticultural Bioscience, Pusan National University
2Life and Industry Convergence Research Institute, Pusan National University
1부산대학교 원예생명과학과
2부산대학교 생명산업융합연구원
*교신저자. *Corresponding Author. 
28 February 2015. pp. 93-105
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Abstract

This study was performed to analyze genetic relationships of the four major cucurbitaceous crops including watermelon, melon, cucumber, and squash/pumpkin. Among 120 EST-SSR primer sets selected from the International Cucurbit Genomics Initiative (ICuGI) database, PCR was successful for 51 (49.17%) primer sets and 49 (40.8%) primer sets showed polymorphisms among eight Cucurbitaceae accessions. A total of 382 allele-specific PCR bands were produced by 49 EST-SSR primers from 24 Cucurbitaceae accessions and used for analysis of pairwise similarity and dendrogram construction. Assessment of the genetic relationships resulted in similarity indexes ranging from 0.01 to 0.85. In the dendrogram, 24 Cucurbitaceae accessions were classified into two major groups (Clade I and II) and 8 subgroups. Clade I comprised two subgroups, Clade I-1 for watermelon accessions [I-1a and I-1b-2: three wild-type watermelons (Citrullus lanatus var. citroides Mats. & Nakai), I-1b-1: six watermelon cultivars (Citrullus lanatus var. vulgaris Schrad.)] and Clade I-2 for melon and cucumber accessions [I-2a-1: 4 melon cultivars (Cucumis melo var. cantalupensis Naudin.), I-2a-2: oriental melon cultivars (Cucumis melo var. conomon Makino.), and I-2b: five cucumber cultivars (Cucumis sativus L.)]. Squash and pumpkin accessions composed Clade II {II-1: two squash/ pumpkin cultivars [Cucurbita moschata (Duch. ex Lam.)/Duch. & Poir. and Cucurbita maxima Duch.] and II-2: two squash/pumpkin cultivars, Cucurbita pepo L./Cucurbita ficifolia Bouche.}. These results were in accordance with previously reported classification of Cucurbitaceae species, indicating that watermelon EST-SSRs show a high level of marker transferability and should be useful for genetic study in other cucurbit crops.

Keywords
comparative genetics
DNA fingerprinting
genetic distance
genetic diversity

본 연구는 수박의 EST-SSR 마커를 이용하여, 네 개의 주요 박과(Cucurbitaceae) 작물인 수박, 호박, 오이, 멜론의 유연관계를 분석하고 마커의 타 박과 작물에 활용 가능성을 알아보기 위해 수행되었다. Cucurbit Genomics Initiative(ICuGI) database로부터 선발된 120 EST-SSR 프라이머 중 51(49.17%)가 PCR이 성공하였고, 49(40.8%)가 8개 박과 유전자원에서 다형성을 보였다. 총 24개 박과 유전자원을 24개 EST-SSR 프라이머로 분석한 결과 총 382개 대립유전자 특이적 PCR 밴드를 얻었으며, 이를 토대로 짝유사행렬과 계통도를 작성하였다. 짝유사행렬의 범위는 0.01-0.85였으며, 작성된 계통도에서 24개 유전자원이 두 개의 주요그룹(Clade I, II)으로 분류되었다. Clade I은 다시 수박으로 구성된 하위집단 I-1 [I-1a, I-1b-2: 각 1개와 2수박 야생종(Citrullus lanatus var. citroides Mats. & Nakai)으로 구성, I-1b-1: 6개수박 재배종(Citrullus lanatus var. vulgaris Schrad.)로 구성]과 멜론과 오이로 구성된 하위집단I-2[I-2a-1: 4개 멜론 재배종(Cucumis melo var. cantalupensis Naudin.), I-2a-2: 2개 참외 재배종(Cucumis melo var. conomon Makino.), I-2b: 5개 오이 재배종(Cucumis sativus L.)]로 분류되었다. 호박으로 구성된 Clade II는 다시 Cucurbita moschata(Duch. ex Lam.) Duch. & Poir와 Cucurbita maxima Duch.로 구성된 하위집단 II-1과 Cucurbita pepo L.과 Cucurbita ficifolia Bouche로 구성된 하위집단 II-2로 나누어졌다. 이러한 결과는 기존의 종명법에 따른 분류와 일치하며, 따라서 수박 EST-SSR 마커를 이용한 타 박과 작물의 비교 유전체 등 연구분야에 적용 가능성을 확인하였다.

키워드
비교 유전학
DNA 지문법
유전적 거리
유전적 다양성
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 33
  • No :1
  • Pages :93-105
  • Received Date : 2014-07-19
  • Revised Date : 2014-09-23
  • Accepted Date : 2014-10-10
  • DOI :https://doi.org/10.7235/hort.2015.14120
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