Investigation of the Post-fertilization Barrier and Appropriate Culture Time During the Interspecific Hybridization of Hydrangea arborescens ‘Pink Annabelle’ and H. paniculata ‘Polar Bear’

SeongHwa Bak; Tae-Ho Han

doi:10.7235/HORT.20230020

All Issue

2023 Vol.41, Issue 2 Preview Page Next Page

Research Article

Investigation of the Post-fertilization Barrier and Appropriate Culture Time During the Interspecific Hybridization of Hydrangea arborescens ‘Pink Annabelle’ and H. paniculata ‘Polar Bear’ Hydrangea arborescens ‘Pink Annabelle’과 H. paniculata ‘Polar Bear’의 종간 교배에서 수정 후 장벽 확인과 적정 배양 시기 구명

30 April 2023. pp. 214-223

PDF XML

Abstract

Interspecific hybridization, referring to the transfer of various traits into another species, is an important method to expand diversity in flowering plants. It is very difficult to form seeds and obtain seedlings due to various factors. Post-fertilization barriers can be overcome by embryo rescue strategies in general. The purpose of this study is to establish a protocol to improv the success of the interspecific hybridization of hydrangeas by identifying seed abortion and optimal ovule stages in the presence of post-fertilization barriers. Hydrangea arborescens and H. paniculata were crossed, and ovaries and seeds were observed every week from three weeks after pollination (WAP). Ovules were cultured in vitro from 5 WAP. As a result, it appeared that the ovaries had matured normally. Embryos in the ovary started to form between 4 and 5 WAP and developed between 5 and 7 WAP. Forty five out of 2,905 cultured ovules germinated, indicating a very low germination rate of 1.6%. Early embryos from 5 to 7 WAP, the early stages of embryo formation and development, had a low germination rate and a long germination period (average of 23.3 days). The germination rate was relatively high and the period for germination (average 11.6 days) was relatively short in cultured ovules from 8 WAP. A sharp increase in the germination period (33 days) and ovule collapse were observed at 14 WAP. In conclusion, post-fertilization barriers appeared to occur between 13 and 14 WAP, and the optimal period for the culturing of ovules was between 8 and 12 WAP for interspecific hybridization (H. arborescens x H. paniculata).

Keywords

cross-breeding

embryo degeneration

embryo development

ovule culture

postzygotic barrier

종간 교배는 화훼식물에서 다양한 형질들을 여러 종에 도입하여 다양성을 확대하는데 중요한 수단이다. 여러가지 요인에 의해 종자 형성 및 실생 획득은 매우 어렵지만 그 중 수정 후 장벽은 배주 배양을 통해 극복 가능하다. 본 연구는 수국 종간 교잡 시(H. arborescens x H. paniculata)에 수정 후 장벽을 확인하고 퇴화 시점과 적정 배주 성숙 단계를 구명하여 수국의 교잡종 획득률 향상을 통한 유전적 다양성 확대의 기반을 마련하고자 한다. H. arborescens x H. paniculata를 교배하였고, 수분 후 3주부터 1주일 단위로 자방과 종자를 관찰하였다. 수분 후 5주부터 배주 배양을 시행하였다. 그 결과 자방은 자연스러운 자방의 성숙 단계로 관찰되었다. 배주는 수분 후 4–5주 사이에 배가 형성되고 수분 후 5–7주에 발달하고 발달이 완료되는 것으로 확인되었다. 배주 배양 전체 2,905개중 45개 발아하였고 평균 발아율은 1.6%로 매우 낮았다. 수분 후 5–7주는 배의 형성과 발달기로 상대적으로 낮은 발아율과 발아 소요 일이 길었다. 수분 후 8주부터는 상대적으로 높은 발아율과 발아 소요 일(평균 11.6일)이 짧았다. 하지만 수분 후 14주에 급격한 발아 소요 일(33일) 증가가 확인되었고 수분 후 14주 배주에서 종자의 함몰이 확인됨에 따라 수분 후 13–14주 사이에 퇴화가 시작되는 것으로 확인되었다. 결론적으로, H. arborescens x H. paniculata의 종간 교배시 수정 후 장벽으로 인한 종자 퇴화는 13–14주에 발생되며, 적정 배주 배양 시기는 8–12주이다.

키워드

교배 육종

배 퇴화

배 발달

배주 배양

접합 후 장벽

References

Bak SH, Han TH (2022) Development of an interspecific hybrid between Hydrangea arborescens 'Magical Pinkerbell' and H. quercifolia JNHQ01. Hortic Sci Technol 40:338-347

Bak SH, Jung HJ, Lim JM, Lee JS, Han, TH (2021b) The New Domestic Hydrangea macrophylla Cultivar 'Moonlight'. Hortic Sci Technol 39:832-837. doi:10.7235/HORT.20210073 10.7235/HORT.20210073

Bak SW, Lim JM, Han TH (2021a) Inheritance of Hydrangea Inflorescence and Leaf Shape between Various Cross Combinations. Flower Res J 29:247-255. doi:10.11623/frj.2021.29.4.05 10.11623/frj.2021.29.4.05

Buitendijk JH, Pinsonneaux N, Van Donk AC, Ramanna MS, Van Lammeren AAM (1995) Embryo rescue by half-ovule culture for the production of interspecific hybrids in Alstroemeria. Sci Hortic 64:65-75. doi:10.1016/0304-4238(95)00827-2 10.1016/0304-4238(95)00827-2

Cai M, Wang K, Luo L, Pan HT, Zhang QX, Yang YY (2015) Production of interspecific hybrids between Hydrangea macrophylla and Hydrangea arborescens via ovary culture. HortScience 50:1765-1769. doi:10.21273/HORTSCI.50.12.1765 10.21273/HORTSCI.50.12.1765

Cerbah M, Mortreau E, Brown S, Siljak-Yakovlev S, Bertrand H, Lambert C (2001) Genome size variation and species relationships in the genus Hydrangea. Theor Appl Genet 103:45-51. doi:10.1007/s001220000529 10.1007/s001220000529

Dirr MA (1998) Manual of woody landscape plants. Their identification, ornamental characteristics, culture, propagation and uses. Stipes Publ CO, Champaign, Illinois USA, p 554

Funamoto T, Ogawa M (2002) A cytogeographical study in Hydrangea paniculata Sieb.(Saxifragaceae sl) in Japan. Chromosome Sci 6:73-82

Funamoto T, Tanaka R (1988) Karyomorphological studies in some taxa of Hydrangea from Japan. La Kromosomo, 49:1583-1594

Jones KD, Reed SM (2006) Production and Verification of Hydrangea arborescens 'Dardom'× H. involucrata Hybrids. HortScience 41:564-566. doi:10.21273/HORTSCI.41.3.564 10.21273/HORTSCI.41.3.564

Jones KD, Reed SM, Rinehart TA (2006) Wide crosses in the Hydrangeaceae: Dichroa febrifuga× Hydrangea macrophylla. In Proc Southern Nursery Assn Res Conf 51:577-579

Kamvorn W, Techawongstien S, Techawongstien S, Theerakulpisut P (2014) Compatibility of inter-specific crosses between Capsicum chinense Jacq. and Capsicum baccatum L. at different fertilization stages. Sci Hortic 179:9-15. doi:10.1016/j.scienta.2014.09.003 10.1016/j.scienta.2014.09.003

Korea Agro-Fisheries & Food Trade Corporation (aT) (2020) Total transaction information by item. Accessed August 2021, https://flower.at.or.kr/hab04/hab04.do

Kudo N, Matsui T, Okada T (2008) A novel interspecific hybrid plant between Hydrangea scandens ssp. chinensis and H. macrophylla via ovule culture. Plant Biotech 25:529-533 doi:10.5511/plantbiotechnology.25.529 10.5511/plantbiotechnology.25.529

Kudo N, Niimi Y (1999) Production of Interspecific Hybrids between Hydrangea macrophylla f. hortensia (Lam.) Rehd. and H. arborescens L. J Jpn Soc Hortic Sci 68:428-439. doi:10.2503/jjshs.68.428 10.2503/jjshs.68.428

Lancaster N, Wesley W (2008) Hydrangea paniculata. RHS Plant Trials Bulletin, London, UK, 2:2-15

Langton FA (1987) Breeding for improved ornamental plants. Improving vegetatively propagated crops. Springer, Dordrecht, Netherlands, pp 159-180

Laojunta T, Narumi-Kawasaki T, Takamura T, Fukai S, (2019) A new interspecific hybrid of Torenia obtained through ovule culture. Hortic Environ Biotechnol 60:443-452. doi:10.1007/s13580-019-00136-6 10.1007/s13580-019-00136-6

Leeson T, Bale S, Jones RT, Dunwell W, McNiel R (2004) Extended vase life for cut stems of Hydrangea paniculata. In Proc Southern Nursery Res Conf 49:624-626

Mendoza CG, Wanke S, Goetghebeur P, Samain MS (2013) Facilitating wide hybridization in Hydrangea sl cultivars: a phylogenetic and marker-assisted breeding approach. Mol Breed 32:233-239. doi:10.1007/s11032-012-9822-8 10.1007/s11032-012-9822-8

Milutinovic M, Lindsey III BE, Wijeratne A, Hernandez JM, Grotewold N, Fernández V, Brkljacic J (2019) Arabidopsis EMSY-like (EML) histone readers are necessary for post-fertilization seed development, but prevent fertilization-independent seed formation. Plant Science 285:99-109 doi:10.1016/j.plantsci.2019.04.007 10.1016/j.plantsci.2019.04.00731203898

Owen JS, Fulcher A, LeBude A, Chappell M (2016) Hydrangea production: Cultivar selection and general practices to consider when propagating and growing hydrangea. UT Ext doi:10.13140/RG.2.2.18534.01608 10.13140/RG.2.2.18534.01608

Reed SM (2000) Development of an in ovolo embryo culture procedure for Hydrangea. J Environ Hortic 18:34-39. doi:10.24266/0738-2898-18.1.34 10.24266/0738-2898-18.1.34

Reed SM, Jones KD, Rinehart TA (2008) Production and characterization of intergeneric hybrids between Dichroa febrifuga and Hydrangea macrophylla. J Am Soc Hortic Sci 133:84-91. doi:10.21273/JASHS.133.1.84 10.21273/JASHS.133.1.84

Reed SM, Riedel GL, Pooler MR (2001) Verification and establishment of Hydrangea macrophylla 'Kardinal' x H. paniculata 'Brussels Lace' interspecific hybrids. J Environ Hortic 19:85-88 doi:10.24266/0738-2898-19.2.85 10.24266/0738-2898-19.2.85

Reed SM, Rinehart TA (2009) Simple-sequence repeat marker analysis of genetic relationships within Hydrangea paniculata. HortScience 44:27-31. doi:10.21273/HORTSCI.44.1.27 10.21273/HORTSCI.44.1.27

Sax K (1931) Chromosome numbers in the ligneous Saxifragaceae. J Arnold Arbor 12:198-206. doi:10.5962/p.324566 10.5962/p.324566

Van Laere K, Van Huylenbroeck J, Van Bockstaele E (2008) Karyotype analysis and physical mapping of 45S rRNA genes in Hydrangea species by fluorescence in situ hybridization. Plant Breed 127:301-307. doi:10.1111/j.1439-0523.2007.01456.x 10.1111/j.1439-0523.2007.01456.x

Yoon JB, Yang DC, Do JW, Park HG (2006) Overcoming two post-fertilization genetic barriers in interspecific hybridization between Capsicum annuum and C. baccatum for introgression of anthracnose resistance. Breed Sci 56:31-38. doi:10.1270/jsbbs.56.31 10.1270/jsbbs.56.31

Zonneveld BJM (2004) Genome size in Hydrangea. Encyclopedia of hydrangeas. Timber Press. Oregon, Portland, pp 245-251

Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 41
No :2
Pages :214-223
Received Date : 2022-05-09
Revised Date : 2022-10-04
Accepted Date : 2022-10-15
DOI :https://doi.org/10.7235/HORT.20230020

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

All Issue