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2025 Vol.43, Issue 6

Research Article

Inhibition of p-65, COX-2, iNOS and Cytokine Inflammation Signaling Pathway by Purified isoorientin from Oplismenus undulatifolius on Pro-inflammatory Responses

주름조개풀(Oplismenus undulatifolius)에서 분리된 Isoorientin이 Inflammation Signaling Pathway에서 p-65, COX-2, iNOS 및 염증성 Cytokine의 억제에 미치는 영향

Eun-Ho Lee1
,
Chae-Won Han1
,
Dong-Hee Kim2
,
Byung-Oh Kim1
,
In-Kyu Kang3
,
Young-Je Cho1*
이 은호1
,
한 채원1
,
김 동희2
,
김 병오1
,
강 인규3
,
조 영제1*
1School of Food Science & Biotechnology/Research Institute of Tailored Food Technology, Kyungpook National University, Daegu 41566, Korea
2Industrial Growth Support Center, NIKOM, Gyeongsan 38540, Korea
3Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea
1경북대학교 식품공학부/특수식품연구소
2한국한의약진흥원
3경북대학교 원예과학과
*Corresponding Author
31 December 2025. pp. 769-780
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Abstract

Isoorientin is a component ofbeen in medicinal plants. In this research, isoorientin was purified from Oplismenus undulatifolius and its ability to inhibit inflammation was investigated. Cell toxicity was not expressed at concentrations below 100 μg/ mL of isoorientin. iNOS-derived NO and COX-2-derived PTGES2 m-RNA expression inhibition activities by the extracts which were found to be determined by the isoorientin concentration. Also, the cytokine and chemokine inhibitory activities of TNF-α, IL-6, IL-1β and MCP-1 via a pre-inflammatory mechanism were decreased upon an addition of isoorientin. The results here show that isoorientin decreases inflammatory reactions in Raw 264.7 cells with LPS via the regulation of iNOS, COX-2, NO, PTGES2, and cytokines and chemokines, demonstrating that isoorientin from Oplismenus undulatifolius is an excellent functional resource with strong anti-inflammatory activity.

Keywords
anti-inflammation
cell viability
plant source
protein expression
purified compound

야생초인 주름조개풀에 존재하는 isoorientin이 가지는 항염증 효과와 작용 mechanism에 대한 물질 본연의 연구가 부족하여 isoorientin이 염증반응에 관여하는 기전을 밝히고자 시도하였다. Isoorientin의 세포 독성을 측정한 결과, 100µg/mL 농도까지 독성이 없는 것으로 관찰되어 처리 농도 구간을 20–100µg/mL로 선정하였다. LPS로 자극한 Raw 264.7 cell에서 p65, iNOS, COX-2와 같은 염증매개체와 염증성 cytokine 및 chemokine의 발현은 isoorientin에 의하여 농도 의존적으로 억제되는 것을 확인하였다. 따라서 isoorientin에 의한 염증 억제효과는 p65, iNOS와 COX-2 등의 발현을 억제함으로써 염증반응에 관련된 물질인 NO, PTGES2, NF-α, IL-6, IL-1β 및 MCP-1 등의 생성을 억제하는 기작을 나타내는 것으로 확인되었다. 그러므로 주름조개풀에 존재하는 isoorientin은 염증성 질환에서 다양한 염증성 물질의 과발현에 의해 유도되는 염증성 질병의 치료제로 활용할 수 있는 기능성소재로 사용될 수 있을 것으로 판단되며, 주름조개풀의 약리성 기능성 소재로 활용하기 위한 유용한 자료로 활용할 수 있을 것이다.

키워드
주름조개풀
이소오리엔틴
iNOS
COX-2
사이토카인
항염증 효과
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :6
  • Pages :769-780
  • Received Date : 2023-10-25
  • Revised Date : 2024-02-22
  • Accepted Date : 2024-03-14
  • DOI :https://doi.org/10.7235/HORT.20250002
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