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2023 Vol.41, Issue 3 Preview Page

Research Article

Quantitative Analysis of Cannabidiol and Δ9-Tetrahydrocannabinol Contents in Different Tissues of Four Cannabis Cultivars using Gas Chromatography-Mass Spectrometry
Young Mo Koo1†
,
S. M. Ahsan1†
,
Da Bin Kwon1†
,
Hyuk Jung2
,
Jeong Doo Choi2
,
Myung Soo Lee2
,
Kiu-Hyung Cho2
,
Byung-Chun In34
,
Hyong Woo Choi14*
1Department of Plant Medicals, Andong National University, Andong 36729, South Korea
2Gyeongbuk Institute for Bio industry, Andong 36618, Korea
3Department of Horticulture and Breeding, Andong National University, Andong 36729, Korea
4Institute of Cannabis Biotechnology, Andong National University, Andong 36729, Korea
*Corresponding Author
†These authors contributed equally to this work.
30 June 2023. pp. 339-348
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Abstract

In this study, we analyzed cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) contents in different tissues of four cannabis cultivars, Cheongsam, Cherry Blossom, Hot Blonde, and Queen Dream, using gas chromatography-mass spectrometry (GC-MS). Standard calibration curves for CBD and THC were generated by analyzing standard CBD and THC solutions via GC-MS, respectively. GC-MS analyses of different tissues of the Cheongsam cultivar revealed different levels of CBD and THC depending on the tissues tested. Female flowers accumulated the highest CBD and THC levels at 0.052±0.02 and 0.02±0.005%, respectively, in the Cheongsam cultivar. Similarly, female flowers of the Cherry Blossom, Hot Blonde, and Queen Dream cultivars accumulated the highest amounts of CBD (2.45% to 3.54%) and THC (0.15% to 0.18%) compared to other tissues, such as the leaves and stems. These results suggest that female flowers of cannabis plants show higher potency levels for CBD and THC production compared to other tissues.

Keywords
Cannabis sativa L.
cannabinoids
ethanol extraction
hemp
phytocannabinoids
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 41
  • No :3
  • Pages :339-348
  • Received Date : 2023-01-31
  • Revised Date : 2023-04-17
  • Accepted Date : 2023-05-05
  • DOI :https://doi.org/10.7235/HORT.20230031
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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