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2021 Vol.39, Issue 4 Preview Page

Research Article

Functional Properties of Newly Bred ‘Summer Prince’ Apples

신육성품종 썸머프린스 사과의 기능적 특성

Eun-Ho Lee1
,
Hye-Jin Park1
,
In-Kyu Kang2
,
Young-Je Cho1*
이 은호1
,
박 혜진1
,
강 인규2
,
조 영제1*
1School of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea
2Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea
1경북대학교 식품공학부
2경북대학교 원예과학과
*Corresponding Author
31 August 2021. pp. 530-545
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Abstract

Apples are one of the most commonly eaten fruits in the world. Therefore, we studied the functionality of apple, a natural raw material that does not have toxicity or sensitivity problems. In this study, ‘Summer Prince’ apple cultivar, newly bred in Korea, was selected as a material, and the experiment was conducted by controlling the total phenolic content (TPC) at various concentrations. The experiment was confirmed by measuring TPC, anti-oxidant activity, and physiological-related enzyme inhibition activity. The peel and whole flesh of ‘Summer Prince’ apples were extracted using water and ethanol, and each extract demonstrated relatively high levels of TPC at 6.83 and 11.11 g·kg-1, and 3.41 and 4.65 g·kg-1, respectively. In the peel and whole extracts, 60 and 50% ethanol was measured. The anti-oxidant activity was measured in 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), anti-oxidant protection factor (PF), and thiobarbituric acid reactive substance (TBARs) assays. Both ‘Summer Prince’ peel and whole extract showed significant effects, but DPPH and ABTS, which showed water-soluble anti-oxidant effects, had better effects than positive control. Therefore, ‘Summer Prince’ apple extracts possess substantial anti-oxidant properties, and physiological- related enzyme inhibition activity was expected. Since inhibitors of enzymes involved in diseases are important factors in terms of drug availability, the ‘Summer Prince’ peel and whole extracts at 50－200 µg·mL-1 concentration were used to investigate the inhibitory effect. The expression levels of enzyme inhibitions activities were decreased in a concentration-dependent manner when ‘Summer Prince’ extracts were treated. These results support the high potential for using these extracts in functional food and cosmetics applications, as the ‘Summer Prince’ apples were determined to have various anti-oxidation, anti-inflammation, anti-wrinkle, and anti-diabetic functions.

Keywords
anti-diabetic
anti-inflammation
anti-oxidation
anti-wrinkle
phenolic compound
‘Summer Prince’ apple

본 연구에서는 국내에서 새롭게 육성한 썸머프린스 사과 품종을 소재로 선정하고, 유용성분인 페놀 함량을 측정하여 항산화 효과와 기능성을 확인하였다. 썸머프린스 사과의 껍질과 whole 사과는 물과 에탄올을 이용하여 추출하였으며, 추출물은 각각 6.83, 11.11g·kg-1, 3.41, 4.65g·kg-1에서 상대적으로 높은 수준의 페놀 함량을 나타내었다. 껍질과 whole의 에탄올 농도별 추출물에서는 각각 60, 50% 농도에서 가장 높은 함량을 나타내었으며, 항산화 활성은 DPPH, ABTS, PF와 TBARs로 측정하였다. 썸머프린스 껍질과 whole 추출물 모두 우수한 효과를 나타내었으며, 수용성 항산화 효과를 나타내는 실험인 DPPH와 ABTS는 대조군인 BHT보다 우수한 효과를 보였다. 따라서 ‘썸머프린스’ 사과 추출물은 노화 방지 효과를 가진다는 것을 확인하였다. Astringent 효과, 그리고 TYR, elastase, collagenase의 저해 효과를 통해 썸머프린스 사과가 피부 노화 방지에 대한 효능이 있다고 판단되었다. α-Glucosidase와 α-amylase 억제제는 당뇨병 환자의 식후 고혈당증을 조절하기 때문에 썸머프린스 사과의 효능을 확인하기 위해 측정하였으며, HAase와 XOase 억제제는 피부를 보호하고 노화를 방지하는 중요한 요소이기 때문에 페놀 함량을 50－200µg·mL-1로 조절하여 측정한 결과, 농도의존적으로 생리활성에 관여하는 효소들을 억제하였다. 위의 결과에 따라 썸머프린스 사과가 항산화 효과, 항염증, 주름개선, 항당뇨 등 다양한 기능성을 가지는 것을 확인하여 기능성 식품 및 화장품 소재로 사용가능성을 확인하였다.

키워드
항당뇨
항염증
항산화
주름개선
페놀성 화합물
썸머프린스 사과
References
1
Andarwulan N, Shetty K (1999) Phenolic content in differentiated tissue cultures of untransformed and <italic>agrobacterium</italic>-transformed roots of anise (<italic>Pimpinella anisum</italic> L.). J Agric Food Chem 47:1776-1780. doi:10.1021/jf981214r 10.1021/jf981214r
2
Ban SH, Yun WH, Kim GH, Kwon SI, Choi C (2014) Genetic identification of apple cultivars bred in Korea using simple sequence repeat markers. Hortic Environ Biotechnol 55:531-539. doi:10.1007/s13580-014-0017-6 10.1007/s13580-014-0017-6
3
Blois MS (1958) Antioxidant determination by the use of stable free radical. Nature 181:1199-1200. doi:10.1038/1811199a0 10.1038/1811199a0
4
Boyer J, Liu RH (2004) Apple phytochemicals and their health benefits. Nutr J 3:5. doi:10.1186/1475-2891-3-5 10.1186/1475-2891-3-5
5
Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Method Enzymol 52:302-310. doi:10.1016/S0076-6879(78)52032-6 10.1016/S0076-6879(78)52032-6
6
Cargnin ST, Gnoatto SB (2017) Ursolic acid from apple pomace and traditional plants: A valuable triterpenoid with functional properties. Food Chem 220:477-489. doi:10.1016/j.foodchem.2016.10.029 10.1016/j.foodchem.2016.10.029
7
Choi IT, Shim KM, Kim YS, Jung MP, Yun KD, Kim SH (2015) Predicting Harvest Maturity of the 'Fuji' Apple at the Gunwi Province of the South Korea using DTS Phenology Model. Journal of Environmental Science International 24:1547-1550. doi:10.5322/JESI.2015.24.11.1547 10.5322/JESI.2015.24.11.1547
8
Choi MH, Shin HJ (2016) Anti-melanogenesis effect of quercetin. Cosmetics 3:18. doi:10.3390/cosmetics3020018 10.3390/cosmetics3020018
9
Christian O, Christoph VS (2013) The role of photolabile dermal nitric oxide derivates in ultraviolet radiation (UVR)-induced cell death. Int J Mol Sci 14:191-204. doi:10.3390/ijms14010191 10.3390/ijms14010191
10
Chung JH, Seo JY, Choi HR, Lee MK, Youn CS, Rhie G, Cho KH, Kim KH, Park KC, et al. (2001) Modulation of skin collagen metabolism in aged and photoaged human skin <italic>in vivo</italic>. J Invest Dermatol 117:1218-1224. doi:10.1046/j.0022-202x.2001.01544.x 10.1046/j.0022-202x.2001.01544.x
11
Davey MW, Keulemans J (2009) Nutritional enhancement in apple: Identification of QTLs for fruit vitamin C content and their stability over different production years. Acta Hortic 814:591-598. doi:10.17660/ActaHortic.2009.814.100 10.17660/ActaHortic.2009.814.100
12
Davidson PM, Parish ME (1989) Methods for testing the efficacy of food antimicrobials. Food Technol 43:148-155
13
Dorfman A, Ott ML (1948) A turbidimetric method for the assay of hyaluronidase. J Biol Chem 172:367-375
14
Folin O, Denis W (1912) On phoshotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12:239-243. doi:10.1016/S0021-9258(18)88697-5 10.1016/S0021-9258(18)88697-5
15
Ghosh P (1994) The role of hyaluronic acid (hyaluronan) in health and disease: interactions with cells, cartilage and components of synovial fluid. Clin Exp Rheumatol 12:75-82
16
Guang D, Till F, Petra Z, Ariane M, Susanne G, Berit R, Jens G, So T, Helmut H, et al. (2007) Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases. Am J Pathol 171:1451-1461. doi:10.2353/ajpath.2007.070136 10.2353/ajpath.2007.070136
17
Gulcin I (2012) Antioxidant activity of food constituents: an overview. Arch Toxicol 86:345-391. doi:10.1007/s00204-011-0774-2 10.1007/s00204-011-0774-2
18
Harada H, Takahashi M (2007) CD44-dependent intracellular and extracellular catabolism of hyaluronic acid by hyaluronidase-1 and -2. J Biol Chem 282:5597-5607. doi:10.1074/jbc.M608358200 10.1074/jbc.M608358200
19
Hong ST (2020) A study on the quality safety of apples by region using MA packaging materials. J Kor Saf Manag Sci 22:1-6. doi:10.12812/ksms.2020.22.2.001
20
Ignat I, Volf I, Popa VI (2011) A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem 126:1821-1835. doi:10.1016/j.foodchem.2010.12.026 10.1016/j.foodchem.2010.12.026
21
Imokawa G, Mishima Y (1980) Isolation and characterization of tyrosinase inhibitors and their differential action on melanogenic subcellular compartment in amelanotic and melanomas. Br Japan Dermatol 103:625-633. doi:10.1111/j.1365-2133.1980.tb01684.x 10.1111/j.1365-2133.1980.tb01684.x
22
Imokawa G, Mishima Y (1981) Biochemical characterization of tyrosinase inhibitors using tyrosinase binding affinity chromatography. Br Japan Dermatol 104:531-539. doi:10.1111/j.1365-2133.1981.tb08167.x 10.1111/j.1365-2133.1981.tb08167.x
23
Jeong Ch, Choi SG, Heo HJ (2008) Analysis of nutritional components and evaluation of functional activities of Sasa borealis leaf tea. Kor J Food Sci Technol 40:586-592
24
Kalinowska M, Bielawska A, Lewandowska-Siwkiewicz H, Priebe W, Lewandowski W (2014) Apples: Content of phenolic compounds vs. variety, part of apple and culti- vation model, extraction of phenolic compounds, biological properties. Plant Physiol Biochem 84:169-188. doi:10.1016/j.plaphy.2014.09.006 10.1016/j.plaphy.2014.09.006
25
Kim KH, Roh SG, Li CR, Jin CF, Kim A, Choi WC (2008) Antidiabetic effects of banaba leaf extracts (<italic>Lagerstroemia speciosa</italic> Pers.) through solvents. J Life Sci 18:1305-1311. doi:10.5352/JLS.2008.18.9.1305 10.5352/JLS.2008.18.9.1305
26
Kim YS, Choi HD, Choi IW (2011) Antioxidative activities of Korean apple polyphenols. J Food Sci Nutr 16:370-375. doi:10.3746/jfn.2011.16.4.370 10.3746/jfn.2011.16.4.370
27
Kraunsoe JA, Claridge TD, Lowe G (1996) Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochem 35:9090-9096. doi:10.1021/bi953013b 10.1021/bi953013b
28
Kwon OJ (2016) Antioxidant and tyrosinase inhibitory activities of immature fruits of <italic>Malus pumila</italic> cv. Fuji. Kor J Food Preserv 23:585-590. doi:10.11002/kjfp.2016.23.4.585 10.11002/kjfp.2016.23.4.585
29
Kwon SI, Kim JI, Kim MJ, Paek PN, Shin YU, Hwang JH, Kang SJ, Kim DI, Choi C, et al. (2011) 'Hongan', a new mid-season apple cultivar. Korean J Hortic Sci Technol 29:655-658
30
Kwon SI, Kwon YS, Park JT, Kim MJ, Kim JH (2016) New columnar apple variety 'Tinkerbell' for pollenizer. Protected Hortic Plant Fac 25:173-176. doi:10.12791/KSBEC.2016.25.3.173 10.12791/KSBEC.2016.25.3.173
31
Kwon SI, Park JT, Lee JW, Kim MJ, Kim JH (2015) 'Picnic', a new mid-season apple cultivar with medium size and good taste. Korean J Hortic Sci Technol 33:784-788. doi:10.7235/hort.2015.14182 10.7235/hort.2015.14182
32
Lee EH, Kim BO, Cho YJ (2017) Inhibitory activities on biological enzymes of extracts from <italic>Oplismenus undulatifolius</italic>. J Appl Biol Chem 60:101-108. doi:10.3839/jabc.2017.017 10.3839/jabc.2017.017
33
Lee EH, Kim YJ, Kwon SI, Kim JH, Kang IK, Jung HY, Park KI, Cho YJ (2018a) Anti-oxidative, health functional, and beauty food activities of extract from newly bred Ruby S apple (<italic>Malus pumila</italic> Mill.) peel. J Kor Soc Food Sci Nutr 47:1093-1102. doi:10.3746/jkfn.2018.47.11.1093 10.3746/jkfn.2018.47.11.1093
34
Lee EH, Kim YJ, Kwon SI, Kim JH, Kang IK, Kim BO, Cho YJ (2018b) Functional properties of newly bred Green ball apple (<italic>Malus pumila</italic> Mill). Kor J Food Preserv 25:837-845. doi:10.11002/kjfp.2018.25.7.837 10.11002/kjfp.2018.25.7.837
35
Lee EH, Lee SY, Jung HY, Kang IK, Ahn DH, Cho YJ (2020)Photo-aging regulation effects of newly bred Green ball apple. J Appl Biol Chem 63:75-82. doi:10.3839/jabc.2020.010 10.3839/jabc.2020.010
36
Lee JT, Jeong YS, An BJ (2002) Physiological activity of <italic>Salicornia herbacea</italic> and its application for cosmetic materials. Kor J Herbology 17:51-60
37
Lee SJ, Song EJ, Kim KBWR, Lee CJ, Jung JY, Kwak JH, Choi MK, Kim MJ, Kim TW, et al. (2010) Inhibitory effects of <italic>Sargassum thunbergii</italic> ethanol extract against α-amylase. Kor J Fish Aquat Sci 43:648-653. doi:10.5657/kfas.2010.43.6.648 10.5657/kfas.2010.43.6.648
38
Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y (2015) The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci 16:26087-26124. doi:10.3390/ijms161125942 10.3390/ijms161125942
39
Lin LY, Huang CY, Chen KC, Peng RY (2021) Pomelo fruit wastes are potentially valuable antioxidants, anti-inflammatories, antihypertensives, and antihyperglycemics. Hortic Environ Biotechnol 62:377-395. doi:10.1007/s13580-020-00325-8 10.1007/s13580-020-00325-8
40
Meyer K (1947) The biological significance of hyaluronic acid and hyaluronidase. Physiol Rev 27:335-359. doi:10.1152/physrev.1947.27.3.335 10.1152/physrev.1947.27.3.335
41
Okuda K (1986) Astringent effects of plants component. Fragrance J 6:270-274
42
Pellegrini N, Re R, Yang M, Rice-Evans C (1998) Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Methods Enzymol 299:379-389. doi:10.1016/S0076-6879(99)99037-7 10.1016/S0076-6879(99)99037-7
43
Pillai S, Oresajo C, Hayward J (2005) Ultraviolet radication and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation-a review. Int J Cosmet Sci 27:17-34. doi:10.1111/j.1467-2494.2004.00241.x 10.1111/j.1467-2494.2004.00241.x
44
Seyoum A, Asres K, El-Fiky FK (2006) Structure-radical scavenging activity relationships of flavonoids. Phytochemistry 67:2058-2070. doi:10.1016/j.phytochem.2006.07.002 10.1016/j.phytochem.2006.07.002
45
Shin KS, Park PJ, Boo HO, Ko JY (2003) Chemical components and comparison of biological activities on the fruit of natural bogbunja. Kor J Plant Res 16:109-117
46
Stirpe F, della Corte E (1969) The regulation of rat liver xanthine oxidase. J Biol Chem 244:3855-3863. doi:10.1016/S0021-9258(17)36428-1 10.1016/S0021-9258(17)36428-1
47
Thanan R, Oikawa S, Hiraku Y, Ohnishi S, Ma N, Pinlaor S, Yongvanit P, Kawanishi S, Murata M (2014) Oxidative stress and its significant roles in neurodegenerative diseases and cancer. Int J Mol Sci 16:193-217. doi:10.3390/ijms16010193 10.3390/ijms16010193
48
Tharmalingam S, Alhasawi A, Appanna VP, Lemire J, Appanna VD (2017) Reactive nitrogen species (RNS)-resistant microbes: adaptation and medical implications. Biol Chem 398:1193-1208. doi:10.1515/hsz-2017-0152 10.1515/hsz-2017-0152
49
Tibbot BK, Skadsen RW (1996) Molecular cloning and characterization of a gibberellin-inducible, putative α-glucosidase gene from barley. Plant Mol Biol 30:229-241. doi:10.1007/BF00020110 10.1007/BF000201108616248
50
Vincent J, Hearing JR (1987) Mammalian monophenol monooxygenase (tyrosinase): Purification, properties, and reactions catalyzed. Method Enzymol 142:154-165. doi:10.1016/S0076-6879(87)42024-7 10.1016/S0076-6879(87)42024-7
51
Wang X, Liu C, Wang Q, Liu C, Sun H, Zhang M, Lyu D, Du G (2021) A comparative study on antioxidant, anti‐inflammatory, antimicrobial activities and chemical composition of Pyrus ussuriensis cultivars from northeastern China. Hortic Environ Biotechnol 62:477-491. doi:10.1007/s13580-020-00322-x 10.1007/s13580-020-00322-x
52
Won DH, Han SB, Hwang JP, Kim SJ, Park JN, Park SN (2012) Antioxidative effect and tyrosinase inhibitory activity of <italic>Lindera obtusiloba</italic> Blume extracts. Kor J Cosmet Sci 38:297-304. doi:10.15230/SCSK.2012.38.4.297 10.15230/SCSK.2012.38.4.297
53
Wu J, Gao H, Zhao L, Liao X, Chen F, Wang Z, Hu X (2007) Chemical compositional characterization of some apple cultivars. Food Chem 103:88-93. doi:10.1016/j.foodchem.2006.07.030 10.1016/j.foodchem.2006.07.030
54
Wunsch E, Heidrich HG (1963) Zur quantitativen bestimmung der kollagenase. 333:149-151. doi:10.1515/bchm2.1963.333.1.149 10.1515/bchm2.1963.333.1.149
55
Yaar M, Gilchrest BA (2007) Photoageing: mechanism, prevention and therapy. Br J Dermatol 157:874-887. doi:10.1111/j.1365-2133.2007.08108.x 10.1111/j.1365-2133.2007.08108.x
56
Yim SH, Cho KS, Choi JH, Lee JH, Kim MS, Lee BHN (2016) Effect of various pear cultivars at different fruit development stages on antioxidant and whitening activities. Kor J Food Sci Technol 48:59-65. doi:10.9721/KJFST.2016.48.1.59 10.9721/KJFST.2016.48.1.59
57
Yoo J, Lee J, Kwon SI, Chung KH, Lee DH, Choi IM, Mattheis JP, Kang IK (2016) Differences in ethylene and fruit quality attributes during storage in new apple cultivars. Korean J Hortic Sci Technol 34:257-268. doi:10.12972/kjhst.20160027 10.12972/kjhst.20160027
58
Yoo JG, Kang IK (2020) Effect of cold storage and 1-methylcyclopropene treatment on fruit storage potential of 'Summer Prince' and 'Summer King' apples. Kor J Food Preserv 27:137-144. doi:10.11002/kjfp.2020.27.2.137 10.11002/kjfp.2020.27.2.137
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :4
  • Pages :530-545
  • Received Date : 2020-12-29
  • Revised Date : 2021-03-30
  • Accepted Date : 2021-05-10
  • DOI :https://doi.org/10.7235/HORT.20210048
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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