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2025 Vol.43, Issue 2 Preview Page

Research Article

Evaluation of Antibiotics and Antimicrobial Agents for the Suppression of Contamination in in vitro Cannabis (Cannabis sativa L.) Seedlings
Young-Cheon Kim12†
,
Young Jae Yun13†
,
Zhao Yuxuan13
,
Jeong Hwan Lee123*
1Department of Life Sciences, Jeonbuk National University, Jeonju 54896, Korea
2HOPS Biosciences, Jeonju 54896, Korea
3Department of Bioactive Material Sciences, Jeonbuk National University, Jeonju 54896, Korea
*Corresponding Author
†These authors contributed equally to this work.
30 April 2025. pp. 212-220
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Abstract

Interest in Cannabis (Cannabis sativa L.) plants for secondary metabolite production has increased in recent years. Although Cannabis plant tissue culture methods are crucial for molecular breeding and micropropagation, the lack of an established protocol offering acceptable quality tissue culture methods for aseptic in vitroCannabis seed germination is a major obstacle. Here, we established a combination of hydrogen peroxide (H2O2) and sodium hypochlorite (NaClO) solutions and examined the effects of several antibiotics, specifically carbenicillin, Augmentin, and Timentin, on the successful suppression of contamination in Cannabis plant tissue cultures. Cannabis seeds were treated with a 1% H2O2 solution for one, two, three, and four days and with 0.04%, 0.2%, 0.4%, and 0.8% NaClO for 15 min. A culture medium containing 300 mg/L carbenicillin, 300 mg/L Augmentin, and 300 mg/L Timentin was also used to suppress contamination efficiently. The greatest amount of suppression was obtained upon a treatment with the 1% H2O2 solution for two days and the 0.2% NaClO solution for 15 min; subsequent incubation in a culture medium containing 300 mg/L Timentin was an effective antibiotic for minimizing contaminants without negative effects on Cannabis seedling growth.

Keywords
hydrogen peroxide
sodium hypochlorite
sterilization
timentin
tissue culture
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 43
  • No :2
  • Pages :212-220
  • Received Date : 2024-07-24
  • Revised Date : 2024-10-13
  • Accepted Date : 2024-10-14
  • DOI :https://doi.org/10.7235/HORT.20250021
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